Restriction map for
pFN21ASDA0251
(Circular) (Six-base) MAPSORT of: pFN21ASDA0251.seq Check: 3355 from: 1 to: 7093
pFN21ASDA0251 7093 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4903 278
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4056 4436 1937
Size: 2119 380 4594
Fragments arranged by size:
4594 2119 380
Acc65I G'GTAC_C
Cuts at: 4414 4414
Size: 7093
AclI AA'CG_TT
Cuts at: 1563 5220 5593 1563
Size: 3657 373 3063
Fragments arranged by size:
3657 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3252 3350 3689 5156 6540 6808 1632
Size: 1620 98 339 1467 1384 268 1917
Fragments arranged by size:
1917 1620 1467 1384 339 268 98
AflII C'TTAA_G
Cuts at: 820 1017 4381 820
Size: 197 3364 3532
Fragments arranged by size:
3532 3364 197
AflIII A'CryG_T
Cuts at: 1203 2028 3386 5992 6969 1203
Size: 825 1358 2606 977 1327
Fragments arranged by size:
2606 1358 1327 977 825
AgeI A'CCGG_T
Cuts at: 5924 5924
Size: 7093
AhdI GACnn_n'nnGTC
Cuts at: 5822 5822
Size: 7093
AleI CACnn'nnGTG
Cuts at: 1166 3294 1166
Size: 2128 4965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3407 1184
Size: 2223 4870
AlwNI CAG_nnn'CTG
Cuts at: 4836 6408 4836
Size: 1572 5521
ApaLI G'TGCA_C
Cuts at: 5150 6306 5150
Size: 1156 5937
ApoI r'AATT_y
Cuts at: 1493 2403 4402 4628 4780 6889 1493
Size: 910 1999 226 152 2109 1697
Fragments arranged by size:
2109 1999 1697 910 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5647 160
Size: 872 4615 1606
Fragments arranged by size:
4615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
AvaI C'yCGr_G
Cuts at: 1870 1943 3432 3810 3829 4099 4253 4418 1870
Size: 73 1489 378 19 270 154 165 4545
Fragments arranged by size:
4545 1489 378 270 165 154 73 19
BamHI G'GATC_C
Cuts at: 2654 4423 4790 2654
Size: 1769 367 4957
Fragments arranged by size:
4957 1769 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3340 4414 5869 618
Size: 325 217 627 1553 1074 1455 1842
Fragments arranged by size:
1842 1553 1455 1074 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2253 2261 3177 3786 3959 4288 4412 1391
Size: 862 8 916 609 173 329 124 4072
Fragments arranged by size:
4072 916 862 609 329 173 124 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 2725 4209 928
Size: 971 606 220 1484 3812
Fragments arranged by size:
3812 1484 971 606 220
BbvCI CC'TCA_GC
Cuts at: 3378 4296 3378
Size: 918 6175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2960 2994 5284 5318 1903
Size: 34 1023 34 2290 34 3678
Fragments arranged by size:
3678 2290 1023 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2364 4985 6195 2364
Size: 2621 1210 3262
Fragments arranged by size:
3262 2621 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7093
BfrBI ATG'CAT
Cuts at: 2230 2230
Size: 7093
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5704 136
Size: 107 122 71 631 605 4032 1525
Fragments arranged by size:
4032 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7088 7088
Size: 7093
BlpI GC'TnA_GC
Cuts at: 3176 3780 4510 3176
Size: 604 730 5759
Fragments arranged by size:
5759 730 604
Bme1580I G_kGCm'C
Cuts at: 1790 3037 3255 3386 5154 6310 1790
Size: 1247 218 131 1768 1156 2573
Fragments arranged by size:
2573 1768 1247 1156 218 131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7093
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3179 3242 4824
Size: 416 53 83 399 24 1762 63 1582
Cuts at: 4824 5782 442
Size: 958 1753
Fragments arranged by size:
1762 1753 1582 958 416 399 83 63
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7093
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3307 3844 4201 3196
Size: 111 537 357 6088
Fragments arranged by size:
6088 537 357 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2123 2159 2192 2705 3485 4052 5753
Size: 818 66 36 33 513 780 567 1701
Cuts at: 5753 1239
Size: 2579
Fragments arranged by size:
2579 1701 818 780 567 513 66 36
33
Bpu10I CC'TnA_GC
Cuts at: 3378 4296 3378
Size: 918 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3867 5218 6083 6381 3867
Size: 1351 865 298 4579
Fragments arranged by size:
4579 1351 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3551 3785 4172 5756 882
Size: 617 2052 234 387 1584 2219
Fragments arranged by size:
2219 2052 1584 617 387 234
BsaAI yAC'GTr
Cuts at: 493 1204 3387 4390 4884 493
Size: 711 2183 1003 494 2702
Fragments arranged by size:
2702 2183 1003 711 494
BsaBI GATnn'nnATC
Cuts at: 3447 7087 3447
Size: 3640 3453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4900 5282 5917 275
Size: 53 83 186 936 3367 382 635 1451
Fragments arranged by size:
3367 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2153 3229 3979 4372 5526 5924 6198 6345 6756
Size: 1076 750 393 1154 398 274 147 411
Cuts at: 6756 2153
Size: 2490
Fragments arranged by size:
2490 1154 1076 750 411 398 393 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2899 2929 3429 3459 4072
Size: 30 165 30 1496 30 500 30 613
Cuts at: 4072 4102 1178
Size: 30 4169
Fragments arranged by size:
4169 1496 613 500 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2860 3836 1186
Size: 1075 599 976 4443
Fragments arranged by size:
4443 1075 976 599
BseYI C'CCAG_C
Cuts at: 2658 2929 3316 4289 6296 6792 2658
Size: 271 387 973 2007 496 2959
Fragments arranged by size:
2959 2007 973 496 387 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4092 1163
Size: 573 2356 4164
Fragments arranged by size:
4164 2356 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4543 5304 6332 1540
Size: 275 186 1279 1263 761 1028 2301
Fragments arranged by size:
2301 1279 1263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3177 3786 5154 5239 6310 3177
Size: 609 1368 85 1071 3960
Fragments arranged by size:
3960 1368 1071 609 85
BsmI GAATG_Cn'
Cuts at: 3870 4604 4697 3870
Size: 734 93 6266
Fragments arranged by size:
6266 734 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2261 3037 3177 3255 3386 3786
Size: 399 463 8 776 140 78 131 400
Cuts at: 3786 3959 4288 4412 5154 5239 6310 1391
Size: 173 329 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 776 742 463 400 399 329
173 140 131 124 85 78 8
BspEI T'CCGG_A
Cuts at: 2153 6756 2153
Size: 4603 2490
BspHI T'CATG_A
Cuts at: 4982 6712 4982
Size: 1730 5363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 4448 844
Size: 2689 915 3489
Fragments arranged by size:
3489 2689 915
BsrBI CCG'CTC
Cuts at: 1394 1823 4980 1394
Size: 429 3157 3507
Fragments arranged by size:
3507 3157 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3795 5588 5762 65
Size: 1183 2547 1793 174 1396
Fragments arranged by size:
2547 1793 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5737 5924 1700
Size: 110 3927 187 2869
Fragments arranged by size:
3927 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7093
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7093
BssSI C'ACGA_G
Cuts at: 3292 3785 5153 6165 3292
Size: 493 1368 1012 4220
Fragments arranged by size:
4220 1368 1012 493
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3340 4070 794
Size: 2546 730 3817
Fragments arranged by size:
3817 2546 730
BstBI TT'CG_AA
Cuts at: 2007 5897 2007
Size: 3890 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7093
BstXI CCAn_nnnn'nTGG
Cuts at: 2183 2276 2183
Size: 93 7000
BstYI r'GATC_y
Cuts at: 1972 2654 3226 3367 3634 3942 4423 4790 5175
Size: 682 572 141 267 308 481 367 385
Cuts at: 5175 5192 6644 6730 6742 7088 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 682 572 481 385 367 346
308 267 141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2924 513
Size: 552 957 902 4682
Fragments arranged by size:
4682 957 902 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6354
Fragments arranged by size:
6354 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2188 4609 5403 5423 2097
Size: 91 2421 794 20 3767
Fragments arranged by size:
3767 2421 794 91 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7058
DraI TTT'AAA
Cuts at: 3238 3706 4144 4397 3238
Size: 468 438 253 5934
Fragments arranged by size:
5934 468 438 253
DraIII CAC_nnn'GTG
Cuts at: 4070 4070
Size: 7093
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6100 809
Size: 507 4784 1802
Fragments arranged by size:
4784 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 3801 4540
Size: 54 1425 50 275 18 1447 524 739
Cuts at: 4540 5429 7058 8
Size: 889 1629 43
Fragments arranged by size:
1629 1447 1425 889 739 524 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 4540 1537
Size: 275 1465 1263 4090
Fragments arranged by size:
4090 1465 1263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2436 3507 3728 3918 5023 1353
Size: 768 315 1071 221 190 1105 3423
Fragments arranged by size:
3423 1105 1071 768 315 221 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5680 6054 6200 387
Size: 1064 138 4091 374 146 1280
Fragments arranged by size:
4091 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3175 3784 3175
Size: 609 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2123 2159 2192 2705 3252 3350
Size: 393 425 66 36 33 513 547 98
Cuts at: 3350 3485 3689 4052 5156 5753 6540 6808 1239
Size: 135 204 363 1104 597 787 268 1524
Fragments arranged by size:
1524 1104 787 597 547 513 425 393
363 268 204 135 98 66 36 33
EcoO109I rG'GnC_Cy
Cuts at: 3713 4149 4333 3713
Size: 436 184 6473
Fragments arranged by size:
6473 436 184
EcoRI G'AATT_C
Cuts at: 4402 4780 4402
Size: 378 6715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3932 3932
Size: 7093
FspI TGC'GCA
Cuts at: 5599 5599
Size: 7093
HaeII r_GCGC'y
Cuts at: 1678 4225 6240 1678
Size: 2547 2015 2531
Fragments arranged by size:
2547 2531 2015
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2899 2931 4211 4243 4340
Size: 32 135 32 1327 32 1280 32 97
Cuts at: 4340 4372 5734 5766 5808 5840 1373
Size: 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 1327 1280 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3107 3116 4437 4683 669
Size: 988 281 1169 9 1321 246 3079
Fragments arranged by size:
3079 1321 1169 988 281 246 9
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7093
HpaI GTT'AAC
Cuts at: 4683 4683
Size: 7093
KpnI G_GTAC'C
Cuts at: 4418 4418
Size: 7093
MfeI C'AATT_G
Cuts at: 4692 4692
Size: 7093
MluI A'CGCG_T
Cuts at: 2028 6969 2028
Size: 4941 2152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4115 6207 6391 1305
Size: 2810 2092 184 2007
Fragments arranged by size:
2810 2092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5271
Fragments arranged by size:
5271 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2909 3294 3362 3606 5051 5410
Size: 648 1069 674 385 68 244 1445 359
Cuts at: 5410 5569 518
Size: 159 2042
Fragments arranged by size:
2042 1445 1069 674 648 385 359 244
159 68
MspA1I CmG'CkG
Cuts at: 1606 2942 3328 3337 4509 5186 6334 6579 1606
Size: 1336 386 9 1172 677 1148 245 2120
Fragments arranged by size:
2120 1336 1172 1148 677 386 245 9
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5584
Fragments arranged by size:
5584 957 552
NdeI CA'TA_TG
Cuts at: 387 2292 2816 387
Size: 1905 524 4664
Fragments arranged by size:
4664 1905 524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7093
NotI GC'GGCC_GC
Cuts at: 4540 4540
Size: 7093
NsiI A_TGCA'T
Cuts at: 2232 2232
Size: 7093
NspI r_CATG'y
Cuts at: 1132 1229 3101 3605 4451 5996 1132
Size: 97 1872 504 846 1545 2229
Fragments arranged by size:
2229 1872 1545 846 504 97
PasI CC'CwG_GG
Cuts at: 1348 4280 1348
Size: 2932 4161
PciI A'CATG_T
Cuts at: 5992 5992
Size: 7093
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 1108
Size: 1283 5810
PfoI T'CCnGG_A
Cuts at: 2682 3207 2682
Size: 525 6568
PmeI GTTT'AAAC
Cuts at: 4397 4397
Size: 7093
PmlI CAC'GTG
Cuts at: 3387 3387
Size: 7093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5162 5194 6675 6707 5162
Size: 32 1481 32 5548
Fragments arranged by size:
5548 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4149 4333 4149
Size: 184 6909
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7093
PsiI TTA'TAA
Cuts at: 4663 4663
Size: 7093
PspXI vC'TCGA_Gb
Cuts at: 3829 4253 3829
Size: 424 6669
PstI C_TGCA'G
Cuts at: 830 2890 3897 4445 830
Size: 2060 1007 548 3478
Fragments arranged by size:
3478 2060 1007 548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7093
PvuII CAG'CTG
Cuts at: 2942 3328 3337 2942
Size: 386 9 6698
Fragments arranged by size:
6698 386 9
SacI G_AGCT'C
Cuts at: 3177 3786 3177
Size: 609 6484
SalI G'TCGA_C
Cuts at: 1936 4435 1936
Size: 2499 4594
SanDI GG'GwC_CC
Cuts at: 4333 4333
Size: 7093
SapI GCTCTTCn'nnn_
Cuts at: 3728 3728
Size: 7093
SbfI CC_TGCA'GG
Cuts at: 4445 4445
Size: 7093
ScaI AGT'ACT
Cuts at: 5341 7053 5341
Size: 1712 5381
SfcI C'TryA_G
Cuts at: 826 1045 2886 3893 3948 4441 5576 6257 6448
Size: 219 1841 1007 55 493 1135 681 191
Cuts at: 6448 826
Size: 1471
Fragments arranged by size:
1841 1471 1135 1007 681 493 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7093
SmaI CCC'GGG
Cuts at: 1872 3812 4420 1872
Size: 1940 608 4545
Fragments arranged by size:
4545 1940 608
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 3882 4253 4381 5197 6098
Size: 197 926 1886 53 371 128 816 901
Cuts at: 6098 6360 820
Size: 262 1553
Fragments arranged by size:
1886 1553 926 901 816 371 262 197
128 53
SnaBI TAC'GTA
Cuts at: 493 4390 4884 493
Size: 3897 494 2702
Fragments arranged by size:
3897 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7093
SphI G_CATG'C
Cuts at: 1132 3101 4451 1132
Size: 1969 1350 3774
Fragments arranged by size:
3774 1969 1350
SspI AAT'ATT
Cuts at: 5 52 2275 5017 5
Size: 47 2223 2742 2081
Fragments arranged by size:
2742 2223 2081 47
StuI AGG'CCT
Cuts at: 1517 4319 1517
Size: 2802 4291
StyI C'CwwG_G
Cuts at: 513 1065 2022 4532 513
Size: 552 957 2510 3074
Fragments arranged by size:
3074 2510 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2412 4293 4328 4356 5120 5137 5290
Size: 1255 832 1881 35 28 764 17 153
Cuts at: 5290 5475 5920 325
Size: 185 445 1498
Fragments arranged by size:
1881 1498 1255 832 764 445 185 153
35 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5339 7051 96
Size: 274 80 33 51 1443 3362 1712 138
Fragments arranged by size:
3362 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3429 3461 4072 4104 7053 7085 3429
Size: 32 611 32 2949 32 3437
Fragments arranged by size:
3437 2949 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2310 3767 1316
Size: 994 1457 4642
Fragments arranged by size:
4642 1457 994
XbaI T'CTAG_A
Cuts at: 4429 4429
Size: 7093
XhoI C'TCGA_G
Cuts at: 1943 3829 4253 1943
Size: 1886 424 4783
Fragments arranged by size:
4783 1886 424
XmaI C'CCGG_G
Cuts at: 1870 3810 4418 1870
Size: 1940 608 4545
Fragments arranged by size:
4545 1940 608
XmnI GAAnn'nnTTC
Cuts at: 3858 3915 5222 3858
Size: 57 1307 5729
Fragments arranged by size:
5729 1307 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4901 276
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI