Restriction map for
pFN21ASDA0235
(Circular) (Six-base) MAPSORT of: pFN21ASDA0235.seq Check: 2408 from: 1 to: 7921
pFN21ASDA0235 7921 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5731 278
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 4816 5264 1937
Size: 1056 1823 448 4594
Fragments arranged by size:
4594 1823 1056 448
Acc65I G'GTAC_C
Cuts at: 2752 5242 2752
Size: 2490 5431
AclI AA'CG_TT
Cuts at: 1563 6048 6421 1563
Size: 4485 373 3063
Fragments arranged by size:
4485 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 2751 4172 4842 5984 7368 7636 1632
Size: 963 156 1421 670 1142 1384 268 1917
Fragments arranged by size:
1917 1421 1384 1142 963 670 268 156
AfeI AGC'GCT
Cuts at: 2588 2588
Size: 7921
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7724
AflIII A'CryG_T
Cuts at: 1203 6820 7797 1203
Size: 5617 977 1327
Fragments arranged by size:
5617 1327 977
AgeI A'CCGG_T
Cuts at: 6752 6752
Size: 7921
AhdI GACnn_n'nnGTC
Cuts at: 6650 6650
Size: 7921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2519 2889 3123 3590 3914 4725 1184
Size: 1335 370 234 467 324 811 4380
Fragments arranged by size:
4380 1335 811 467 370 324 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3387 3419 3387
Size: 32 7889
AlwNI CAG_nnn'CTG
Cuts at: 2692 3473 4022 5664 7236 2692
Size: 781 549 1642 1572 3377
Fragments arranged by size:
3377 1642 1572 781 549
ApaI G_GGCC'C
Cuts at: 2597 2597
Size: 7921
ApaLI G'TGCA_C
Cuts at: 5978 7134 5978
Size: 1156 6765
ApoI r'AATT_y
Cuts at: 1493 2365 2666 4308 4883 5230 5456 5608 7717
Size: 872 301 1642 575 347 226 152 2109
Cuts at: 7717 1493
Size: 1697
Fragments arranged by size:
2109 1697 1642 872 575 347 301 226
152
AseI AT'TA_AT
Cuts at: 160 1032 4331 6475 160
Size: 872 3299 2144 1606
Fragments arranged by size:
3299 2144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 5246 1870
Size: 73 115 3188 4545
Fragments arranged by size:
4545 3188 115 73
AvrII C'CTAG_G
Cuts at: 5179 5179
Size: 7921
BamHI G'GATC_C
Cuts at: 3956 5251 5618 3956
Size: 1295 367 6259
Fragments arranged by size:
6259 1295 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2752 3356 5242 6697 618
Size: 325 217 627 965 604 1886 1455 1842
Fragments arranged by size:
1886 1842 1455 965 627 604 325 217
BanII G_rGCy'C
Cuts at: 1391 2597 3400 5240 1391
Size: 1206 803 1840 4072
Fragments arranged by size:
4072 1840 1206 803
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4830 928
Size: 971 2931 4019
Fragments arranged by size:
4019 2931 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5069 5103 6112 6146 1903
Size: 34 3132 34 1009 34 3678
Fragments arranged by size:
3678 3132 1009 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3090 5813 7023 3090
Size: 2723 1210 3988
Fragments arranged by size:
3988 2723 1210
BclI T'GATC_A
Cuts at: 1548 3346 1548
Size: 1798 6123
BfrBI ATG'CAT
Cuts at: 2876 2876
Size: 7921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3498 6532 136
Size: 107 122 71 631 605 1826 3034 1525
Fragments arranged by size:
3034 1826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2219 3812 7916 2219
Size: 1593 4104 2224
Fragments arranged by size:
4104 2224 1593
BlpI GC'TnA_GC
Cuts at: 2889 3156 3715 4284 5338 2889
Size: 267 559 569 1054 5472
Fragments arranged by size:
5472 1054 569 559 267
Bme1580I G_kGCm'C
Cuts at: 1790 2597 5982 7138 1790
Size: 807 3385 1156 2573
Fragments arranged by size:
3385 2573 1156 807
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2630 5652 6610
Size: 416 53 83 399 24 1213 3022 958
Cuts at: 6610 442
Size: 1753
Fragments arranged by size:
3022 1753 1213 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7921
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3798 4293 3798
Size: 495 7426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2916 2961 3207 3384 3396 3495 3897 4044
Size: 1677 45 246 177 12 99 402 147
Cuts at: 4044 4060 4263 6581 1239
Size: 16 203 2318 2579
Fragments arranged by size:
2579 2318 1677 402 246 203 177 147
99 45 16 12
Bpu10I CC'TnA_GC
Cuts at: 4438 4438
Size: 7921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4212 6046 6911 7209 4212
Size: 1834 865 298 4924
Fragments arranged by size:
4924 1834 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2448 6584 882
Size: 617 949 4136 2219
Fragments arranged by size:
4136 2219 949 617
BsaAI yAC'GTr
Cuts at: 493 1204 5026 5218 5712 493
Size: 711 3822 192 494 2702
Fragments arranged by size:
3822 2702 711 494 192
BsaBI GATnn'nnATC
Cuts at: 2538 3961 7915 2538
Size: 1423 3954 2544
Fragments arranged by size:
3954 2544 1423
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5728 6110 6745 275
Size: 53 83 186 936 4195 382 635 1451
Fragments arranged by size:
4195 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6354 6752 7026 7173 7584 6354
Size: 398 274 147 411 6691
Fragments arranged by size:
6691 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 3162 3192 3387
Size: 30 165 30 1360 30 369 30 195
Cuts at: 3387 3417 1178
Size: 30 5682
Fragments arranged by size:
5682 1360 369 195 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 2964 3862 1186
Size: 1267 511 898 5245
Fragments arranged by size:
5245 1267 898 511
BseYI C'CCAG_C
Cuts at: 2196 2802 7124 7620 2196
Size: 606 4322 496 2497
Fragments arranged by size:
4322 2497 606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4816 5371 6132 7160 1540
Size: 275 186 2815 555 761 1028 2301
Fragments arranged by size:
2815 2301 1028 761 555 275 186
BsiHKAI G_wGCw'C
Cuts at: 3400 4542 5982 6067 7138 3400
Size: 1142 1440 85 1071 4183
Fragments arranged by size:
4183 1440 1142 1071 85
BsmI GAATG_Cn'
Cuts at: 5214 5432 5525 5214
Size: 218 93 7610
Fragments arranged by size:
7610 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2597 3400 4542 5240 5982 6067 7138
Size: 399 807 803 1142 698 742 85 1071
Cuts at: 7138 1391
Size: 2174
Fragments arranged by size:
2174 1142 1071 807 803 742 698 399
85
BspEI T'CCGG_A
Cuts at: 7584 7584
Size: 7921
BspHI T'CATG_A
Cuts at: 2029 5810 7540 2029
Size: 3781 1730 2410
Fragments arranged by size:
3781 2410 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 3182 5065 5276 844
Size: 2176 162 1883 211 3489
Fragments arranged by size:
3489 2176 1883 211 162
BsrBI CCG'CTC
Cuts at: 1394 1823 5808 1394
Size: 429 3985 3507
Fragments arranged by size:
3985 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4702 4900 6416 6590 65
Size: 1183 3454 198 1516 174 1396
Fragments arranged by size:
3454 1516 1396 1183 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6565 6752 1700
Size: 110 4755 187 2869
Fragments arranged by size:
4755 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3804 4576 96
Size: 3708 772 3441
Fragments arranged by size:
3708 3441 772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7921
BssSI C'ACGA_G
Cuts at: 5981 6993 5981
Size: 1012 6909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2927 3455 794
Size: 2133 528 5260
Fragments arranged by size:
5260 2133 528
BstBI TT'CG_AA
Cuts at: 2007 6725 2007
Size: 4718 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7921
BstXI CCAn_nnnn'nTGG
Cuts at: 4973 5026 4973
Size: 53 7868
BstYI r'GATC_y
Cuts at: 1972 2219 3812 3956 5251 5618 6003 6020 7472
Size: 247 1593 144 1295 367 385 17 1452
Cuts at: 7472 7558 7570 7916 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1593 1452 1295 385 367 346 247
144 86 17 12
BstZ17I GTA'TAC
Cuts at: 2994 2994
Size: 7921
BtgI C'CryG_G
Cuts at: 513 1065 2700 3068 513
Size: 552 1635 368 5366
Fragments arranged by size:
5366 1635 552 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7182
Fragments arranged by size:
7182 683 56
BtsI GCAGTG_nn'
Cuts at: 3045 4707 5437 6231 6251 3045
Size: 1662 730 794 20 4715
Fragments arranged by size:
4715 1662 794 730 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7886
DraI TTT'AAA
Cuts at: 5225 5225
Size: 7921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6928 809
Size: 507 5612 1802
Fragments arranged by size:
5612 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3521 4138 5139
Size: 54 1425 50 275 18 1691 617 1001
Cuts at: 5139 5368 6257 7886 8
Size: 229 889 1629 43
Fragments arranged by size:
1691 1629 1425 1001 889 617 275 229
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5368 1537
Size: 275 3556 4090
Fragments arranged by size:
4090 3556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 2770 5851 1353
Size: 1281 136 3081 3423
Fragments arranged by size:
3423 3081 1281 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6508 6882 7028 387
Size: 1064 138 4919 374 146 1280
Fragments arranged by size:
4919 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3398 3398
Size: 7921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2595 2751 2916 2961 3207 3384 3396
Size: 393 963 156 165 45 246 177 12
Cuts at: 3396 3495 3897 4044 4060 4172 4263 4842 5984
Size: 99 402 147 16 112 91 579 1142
Cuts at: 5984 6581 7368 7636 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1142 963 787 597 579 402 393
268 246 177 165 156 147 112 99
91 45 16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2403 5190 2403
Size: 2787 5134
EcoO109I rG'GnC_Cy
Cuts at: 2594 2624 5183 2594
Size: 30 2559 5332
Fragments arranged by size:
5332 2559 30
EcoRI G'AATT_C
Cuts at: 5230 5608 5230
Size: 378 7543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3621 3621
Size: 7921
FspI TGC'GCA
Cuts at: 5111 6427 5111
Size: 1316 6605
HaeII r_GCGC'y
Cuts at: 1678 2590 7068 1678
Size: 912 4478 2531
Fragments arranged by size:
4478 2531 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3770 3802 4375 4407 5077
Size: 32 135 32 2198 32 573 32 670
Cuts at: 5077 5109 6562 6594 6636 6668 1373
Size: 32 1453 32 42 32 2626
Fragments arranged by size:
2626 2198 1453 670 573 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3656 4817 4877 5265 5511
Size: 988 281 912 806 1161 60 388 246
Cuts at: 5511 669
Size: 3079
Fragments arranged by size:
3079 1161 988 912 806 388 281 246
60
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7921
HpaI GTT'AAC
Cuts at: 2850 5511 2850
Size: 2661 5260
KpnI G_GTAC'C
Cuts at: 2756 5246 2756
Size: 2490 5431
MfeI C'AATT_G
Cuts at: 3525 5520 3525
Size: 1995 5926
MluI A'CGCG_T
Cuts at: 7797 7797
Size: 7921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2594 3296 3356 3443 5127 7035 7219 1305
Size: 1289 702 60 87 1684 1908 184 2007
Fragments arranged by size:
2007 1908 1684 1289 702 184 87 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5141 10
Size: 54 1425 343 3309 2790
Fragments arranged by size:
3309 2790 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4668 5000 5879 6238 6397 518
Size: 648 3502 332 879 359 159 2042
Fragments arranged by size:
3502 2042 879 648 359 332 159
MspA1I CmG'CkG
Cuts at: 1606 2936 3113 3257 3473 4283 5337 6014 7162
Size: 1330 177 144 216 810 1054 677 1148
Cuts at: 7162 7407 1606
Size: 245 2120
Fragments arranged by size:
2120 1330 1148 1054 810 677 245 216
177 144
NcoI C'CATG_G
Cuts at: 513 1065 2700 3068 513
Size: 552 1635 368 5366
Fragments arranged by size:
5366 1635 552 368
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7921
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7921
NotI GC'GGCC_GC
Cuts at: 5368 5368
Size: 7921
NsiI A_TGCA'T
Cuts at: 2878 2878
Size: 7921
NspI r_CATG'y
Cuts at: 1132 1229 3310 3895 4802 5279 6824 1132
Size: 97 2081 585 907 477 1545 2229
Fragments arranged by size:
2229 2081 1545 907 585 477 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7921
PciI A'CATG_T
Cuts at: 6820 6820
Size: 7921
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5204 1108
Size: 4096 3825
PfoI T'CCnGG_A
Cuts at: 2548 3913 2548
Size: 1365 6556
PmeI GTTT'AAAC
Cuts at: 5225 5225
Size: 7921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3387 3419 5990 6022 7503 7535 3387
Size: 32 2571 32 1481 32 3773
Fragments arranged by size:
3773 2571 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5183 5183
Size: 7921
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7921
PsiI TTA'TAA
Cuts at: 5491 5491
Size: 7921
PspOMI G'GGCC_C
Cuts at: 2593 2593
Size: 7921
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4707 4739 4707
Size: 32 7889
PstI C_TGCA'G
Cuts at: 830 2857 3001 3016 3112 3262 3460 4018 4679
Size: 2027 144 15 96 150 198 558 661
Cuts at: 4679 4714 5273 830
Size: 35 559 3478
Fragments arranged by size:
3478 2027 661 559 558 198 150 144
96 35 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7921
PvuII CAG'CTG
Cuts at: 2936 3113 3257 3473 4283 2936
Size: 177 144 216 810 6574
Fragments arranged by size:
6574 810 216 177 144
SacI G_AGCT'C
Cuts at: 3400 3400
Size: 7921
SalI G'TCGA_C
Cuts at: 1936 4815 5263 1936
Size: 2879 448 4594
Fragments arranged by size:
4594 2879 448
SbfI CC_TGCA'GG
Cuts at: 5273 5273
Size: 7921
ScaI AGT'ACT
Cuts at: 3324 6169 7881 3324
Size: 2845 1712 3364
Fragments arranged by size:
3364 2845 1712
SexAI A'CCwGG_T
Cuts at: 3649 3649
Size: 7921
SfcI C'TryA_G
Cuts at: 826 1045 2640 2778 2853 2997 3012 3108 3258
Size: 219 1595 138 75 144 15 96 150
Cuts at: 3258 3456 3708 4014 4613 4675 4710 5269 6404
Size: 198 252 306 599 62 35 559 1135
Cuts at: 6404 7085 7276 826
Size: 681 191 1471
Fragments arranged by size:
1595 1471 1135 681 599 559 306 252
219 198 191 150 144 138 96 75
62 35 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7921
SmaI CCC'GGG
Cuts at: 1872 5248 1872
Size: 3376 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4227 6025 6926 7188 820
Size: 197 926 2284 1798 901 262 1553
Fragments arranged by size:
2284 1798 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5218 5712 493
Size: 4725 494 2702
Fragments arranged by size:
4725 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7921
SphI G_CATG'C
Cuts at: 1132 3310 3895 5279 1132
Size: 2178 585 1384 3774
Fragments arranged by size:
3774 2178 1384 585
SspI AAT'ATT
Cuts at: 5 52 5845 5
Size: 47 5793 2081
Fragments arranged by size:
5793 2081 47
StuI AGG'CCT
Cuts at: 1517 2289 2784 2925 1517
Size: 772 495 141 6513
Fragments arranged by size:
6513 772 495 141
StyI C'CwwG_G
Cuts at: 513 1065 2290 2700 3068 3391 5179 5360 513
Size: 552 1225 410 368 323 1788 181 3074
Fragments arranged by size:
3074 1788 1225 552 410 368 323 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 3689 3847 5948 5965 6118 6303
Size: 1255 1419 690 158 2101 17 153 185
Cuts at: 6303 6748 325
Size: 445 1498
Fragments arranged by size:
2101 1498 1419 1255 690 445 185 158
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3322 3665 3804
Size: 274 80 33 51 1443 1345 343 139
Cuts at: 3804 4576 4801 6167 7879 96
Size: 772 225 1366 1712 138
Fragments arranged by size:
1712 1443 1366 1345 772 343 274 225
139 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7881 7913 7881
Size: 32 7889
Tth111I GACn'n_nGTC
Cuts at: 1316 3651 1316
Size: 2335 5586
XbaI T'CTAG_A
Cuts at: 4244 5257 4244
Size: 1013 6908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7921
XmaI C'CCGG_G
Cuts at: 1870 5246 1870
Size: 3376 4545
XmnI GAAnn'nnTTC
Cuts at: 3601 4048 4596 6050 3601
Size: 447 548 1454 5472
Fragments arranged by size:
5472 1454 548 447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5729 276
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BsiWI BsmBI Bsu36I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI