Restriction map for
pFN21ASDA0217
(Circular) (Six-base) MAPSORT of: pFN21ASDA0217.seq Check: 1410 from: 1 to: 6943
pFN21ASDA0217 6943 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3922 3922
Size: 6943
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3041 4753 278
Size: 53 83 186 936 1505 1712 2468
Fragments arranged by size:
2468 1712 1505 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4286 1937
Size: 2349 4594
Acc65I G'GTAC_C
Cuts at: 2164 4264 2164
Size: 2100 4843
AclI AA'CG_TT
Cuts at: 1563 5070 5443 1563
Size: 3507 373 3063
Fragments arranged by size:
3507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3408 5006 6390 6658 1632
Size: 1776 1598 1384 268 1917
Fragments arranged by size:
1917 1776 1598 1384 268
AfeI AGC'GCT
Cuts at: 2095 3029 2095
Size: 934 6009
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6746
AflIII A'CryG_T
Cuts at: 1203 2550 5842 6819 1203
Size: 1347 3292 977 1327
Fragments arranged by size:
3292 1347 1327 977
AgeI A'CCGG_T
Cuts at: 5774 5774
Size: 6943
AhdI GACnn_n'nnGTC
Cuts at: 5672 5672
Size: 6943
AleI CACnn'nnGTG
Cuts at: 1166 2600 2639 1166
Size: 1434 39 5470
Fragments arranged by size:
5470 1434 39
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6943
AlwNI CAG_nnn'CTG
Cuts at: 2870 4686 6258 2870
Size: 1816 1572 3555
Fragments arranged by size:
3555 1816 1572
ApaLI G'TGCA_C
Cuts at: 5000 6156 5000
Size: 1156 5787
ApoI r'AATT_y
Cuts at: 1493 2516 2810 2825 3511 4252 4478 4630 6739
Size: 1023 294 15 686 741 226 152 2109
Cuts at: 6739 1493
Size: 1697
Fragments arranged by size:
2109 1697 1023 741 686 294 226 152
15
AseI AT'TA_AT
Cuts at: 160 1032 3356 3360 5497 160
Size: 872 2324 4 2137 1606
Fragments arranged by size:
2324 2137 1606 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
AvaI C'yCGr_G
Cuts at: 1870 1943 2490 4268 1870
Size: 73 547 1778 4545
Fragments arranged by size:
4545 1778 547 73
BamHI G'GATC_C
Cuts at: 4273 4640 4273
Size: 367 6576
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2164 3778 4264 5719 618
Size: 325 217 627 377 1614 486 1455 1842
Fragments arranged by size:
1842 1614 1455 627 486 377 325 217
BanII G_rGCy'C
Cuts at: 1391 2218 3774 4089 4110 4138 4262 1391
Size: 827 1556 315 21 28 124 4072
Fragments arranged by size:
4072 1556 827 315 124 28 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 3662 928
Size: 971 591 1172 4209
Fragments arranged by size:
4209 1172 971 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5134 5168 1903
Size: 34 3197 34 3678
Fragments arranged by size:
3678 3197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3249 4835 6045 3249
Size: 1586 1210 4147
Fragments arranged by size:
4147 1586 1210
BclI T'GATC_A
Cuts at: 1548 2032 3096 1548
Size: 484 1064 5395
Fragments arranged by size:
5395 1064 484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5554 136
Size: 107 122 71 631 605 3882 1525
Fragments arranged by size:
3882 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2666 6938 2666
Size: 4272 2671
BlpI GC'TnA_GC
Cuts at: 2630 4360 2630
Size: 1730 5213
Bme1580I G_kGCm'C
Cuts at: 1790 5004 6160 1790
Size: 3214 1156 2573
Fragments arranged by size:
3214 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3007 3111 1318
Size: 1689 104 5150
Fragments arranged by size:
5150 1689 104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4674 5632 442
Size: 416 53 83 399 24 3257 958 1753
Fragments arranged by size:
3257 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3177 3657 3748 4152 5603 1239
Size: 1938 480 91 404 1451 2579
Fragments arranged by size:
2579 1938 1451 480 404 91
Bpu10I CC'TnA_GC
Cuts at: 2043 2043
Size: 6943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4103 5068 5933 6231 4103
Size: 965 865 298 4815
Fragments arranged by size:
4815 965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3766 4137 5606 882
Size: 617 2267 371 1469 2219
Fragments arranged by size:
2267 2219 1469 617 371
BsaAI yAC'GTr
Cuts at: 493 1204 4240 4734 493
Size: 711 3036 494 2702
Fragments arranged by size:
3036 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6937 6937
Size: 6943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3038 3212 4750 5132
Size: 53 83 186 936 1505 174 1538 382
Cuts at: 5132 5767 275
Size: 635 1451
Fragments arranged by size:
1538 1505 1451 936 635 382 186 174
83 53
BsaWI w'CCGG_w
Cuts at: 3369 3525 5376 5774 6048 6195 6606 3369
Size: 156 1851 398 274 147 411 3706
Fragments arranged by size:
3706 1851 411 398 274 156 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2691 2721 3530 3553 3560
Size: 30 165 30 1288 30 809 23 7
Cuts at: 3560 3583 1178
Size: 23 4538
Fragments arranged by size:
4538 1288 809 165 30 30 30 23
23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3207 3234 3554 4221 1186
Size: 1102 919 27 320 667 3908
Fragments arranged by size:
3908 1102 919 667 320 27
BseYI C'CCAG_C
Cuts at: 2383 3021 3069 3446 3607 3826 3965 4111 6146
Size: 638 48 377 161 219 139 146 2035
Cuts at: 6146 6642 2383
Size: 496 2684
Fragments arranged by size:
2684 2035 638 496 377 219 161 146
139 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3315 3747 3950 1163
Size: 573 1579 432 203 4156
Fragments arranged by size:
4156 1579 573 432 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3915 4157 4393 5154 6182 1540
Size: 275 186 1914 242 236 761 1028 2301
Fragments arranged by size:
2301 1914 1028 761 275 242 236 186
BsiHKAI G_wGCw'C
Cuts at: 4217 5004 5089 6160 4217
Size: 787 85 1071 5000
Fragments arranged by size:
5000 1071 787 85
BsiWI C'GTAC_G
Cuts at: 3807 3807
Size: 6943
BsmI GAATG_Cn'
Cuts at: 2356 4454 4547 2356
Size: 2098 93 4752
Fragments arranged by size:
4752 2098 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3195 3219 4202 3195
Size: 24 983 5936
Fragments arranged by size:
5936 983 24
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 3774 4089 4110 4138 4217 4262
Size: 399 428 1556 315 21 28 79 45
Cuts at: 4262 5004 5089 6160 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1556 1071 742 428 399 315 85
79 45 28 21
BspEI T'CCGG_A
Cuts at: 3369 6606 3369
Size: 3237 3706
BspHI T'CATG_A
Cuts at: 4832 6562 4832
Size: 1730 5213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3383 3922 4298 844
Size: 2539 539 376 3489
Fragments arranged by size:
3489 2539 539 376
BsrBI CCG'CTC
Cuts at: 1394 1823 4830 1394
Size: 429 3007 3507
Fragments arranged by size:
3507 3007 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2357 4016 5438 5612 65
Size: 1183 1109 1659 1422 174 1396
Fragments arranged by size:
1659 1422 1396 1183 1109 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3644 3781 4156 4167 5587 5774 1700
Size: 110 1834 137 375 11 1420 187 2869
Fragments arranged by size:
2869 1834 1420 375 187 137 110 11
BsrGI T'GTAC_A
Cuts at: 96 3060 3087 96
Size: 2964 27 3952
Fragments arranged by size:
3952 2964 27
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6943
BssSI C'ACGA_G
Cuts at: 5003 6015 5003
Size: 1012 5931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 794
Size: 1449 5494
BstBI TT'CG_AA
Cuts at: 2007 5747 2007
Size: 3740 3203
BstEII G'GTnAC_C
Cuts at: 1184 2333 1184
Size: 1149 5794
BstYI r'GATC_y
Cuts at: 1972 2666 4273 4640 5025 5042 6494 6580 6592
Size: 694 1607 367 385 17 1452 86 12
Cuts at: 6592 6938 1972
Size: 346 1977
Fragments arranged by size:
1977 1607 1452 694 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2299 2766 3948 4185 513
Size: 552 1234 467 1182 237 3271
Fragments arranged by size:
3271 1234 1182 552 467 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6204
Fragments arranged by size:
6204 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 3342 3906 4459 5253 5273 2248
Size: 1094 564 553 794 20 3918
Fragments arranged by size:
3918 1094 794 564 553 20
ClaI AT'CG_AT
Cuts at: 1554 3352 1554
Size: 1798 5145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6908
DraI TTT'AAA
Cuts at: 2791 4247 2791
Size: 1456 5487
DraIII CAC_nnn'GTG
Cuts at: 3883 3883
Size: 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5950 809
Size: 507 4634 1802
Fragments arranged by size:
4634 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2284 4154 4182
Size: 54 1425 50 275 18 454 1870 28
Cuts at: 4182 4390 5279 6908 8
Size: 208 889 1629 43
Fragments arranged by size:
1870 1629 1425 889 454 275 208 54
50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 4154 4390 1537
Size: 275 2342 236 4090
Fragments arranged by size:
4090 2342 275 236
EarI CTCTTCn'nnn_
Cuts at: 1353 4873 1353
Size: 3520 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3872 3889 5530 5904 6050 387
Size: 1064 138 2283 17 1641 374 146 1280
Fragments arranged by size:
2283 1641 1280 1064 374 146 138 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3177 3408 3657 3748 4152 5006 5603
Size: 393 1545 231 249 91 404 854 597
Cuts at: 5603 6390 6658 1239
Size: 787 268 1524
Fragments arranged by size:
1545 1524 854 787 597 404 393 268
249 231 91
EcoNI CCTnn'n_nnAGG
Cuts at: 3492 3492
Size: 6943
EcoO109I rG'GnC_Cy
Cuts at: 2411 3305 3717 2411
Size: 894 412 5637
Fragments arranged by size:
5637 894 412
EcoRI G'AATT_C
Cuts at: 2516 3511 4252 4630 2516
Size: 995 741 378 4829
Fragments arranged by size:
4829 995 741 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 6943
FseI GG_CCGG'CC
Cuts at: 4171 4171
Size: 6943
FspI TGC'GCA
Cuts at: 5449 5449
Size: 6943
HaeII r_GCGC'y
Cuts at: 1678 2097 3031 6090 1678
Size: 419 934 3059 2531
Fragments arranged by size:
3059 2531 934 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2095 2127 2892
Size: 32 135 32 445 32 46 32 765
Cuts at: 2892 2924 3553 3585 5584 5616 5658 5690 1373
Size: 32 629 32 1999 32 42 32 2626
Fragments arranged by size:
2626 1999 765 629 445 135 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4287 4533 669
Size: 988 281 2349 246 3079
Fragments arranged by size:
3079 2349 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3598 748
Size: 2850 4093
HpaI GTT'AAC
Cuts at: 4533 4533
Size: 6943
KpnI G_GTAC'C
Cuts at: 2168 4268 2168
Size: 2100 4843
MfeI C'AATT_G
Cuts at: 4542 4542
Size: 6943
MluI A'CGCG_T
Cuts at: 6819 6819
Size: 6943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 3596 6057 6241 1305
Size: 1053 1238 2461 184 2007
Fragments arranged by size:
2461 2007 1238 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2286 10
Size: 54 1425 343 454 4667
Fragments arranged by size:
4667 1425 454 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 2600 2639 4901 5260 5419 518
Size: 648 1183 251 39 2262 359 159 2042
Fragments arranged by size:
2262 2042 1183 648 359 251 159 39
MspA1I CmG'CkG
Cuts at: 1606 4359 5036 6184 6429 1606
Size: 2753 677 1148 245 2120
Fragments arranged by size:
2753 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3646 3783 4158 4169 3646
Size: 137 375 11 6420
Fragments arranged by size:
6420 375 137 11
NcoI C'CATG_G
Cuts at: 513 1065 4185 513
Size: 552 3120 3271
Fragments arranged by size:
3271 3120 552
NdeI CA'TA_TG
Cuts at: 387 3430 387
Size: 3043 3900
NgoMIV G'CCGG_C
Cuts at: 3644 3781 4156 4167 3644
Size: 137 375 11 6420
Fragments arranged by size:
6420 375 137 11
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6943
NotI GC'GGCC_GC
Cuts at: 4390 4390
Size: 6943
NspI r_CATG'y
Cuts at: 1132 1229 2554 3284 4301 5846 1132
Size: 97 1325 730 1017 1545 2229
Fragments arranged by size:
2229 1545 1325 1017 730 97
PacI TTA_AT'TAA
Cuts at: 3360 3360
Size: 6943
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6943
PciI A'CATG_T
Cuts at: 2550 5842 2550
Size: 3292 3651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2987 3167 3635 3883 1108
Size: 1879 180 468 248 4168
Fragments arranged by size:
4168 1879 468 248 180
PfoI T'CCnGG_A
Cuts at: 2076 2076
Size: 6943
PmeI GTTT'AAAC
Cuts at: 4247 4247
Size: 6943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5012 5044 6525 6557 5012
Size: 32 1481 32 5398
Fragments arranged by size:
5398 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2411 3305 3717 2411
Size: 894 412 5637
Fragments arranged by size:
5637 894 412
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6943
PsiI TTA'TAA
Cuts at: 2816 3176 4513 2816
Size: 360 1337 5246
Fragments arranged by size:
5246 1337 360
PstI C_TGCA'G
Cuts at: 830 3349 4295 830
Size: 2519 946 3478
Fragments arranged by size:
3478 2519 946
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6943
SalI G'TCGA_C
Cuts at: 1936 4285 1936
Size: 2349 4594
SanDI GG'GwC_CC
Cuts at: 2411 2411
Size: 6943
SbfI CC_TGCA'GG
Cuts at: 4295 4295
Size: 6943
ScaI AGT'ACT
Cuts at: 2499 5191 6903 2499
Size: 2692 1712 2539
Fragments arranged by size:
2692 2539 1712
SfcI C'TryA_G
Cuts at: 826 1045 3345 3753 4291 5426 6107 6298 826
Size: 219 2300 408 538 1135 681 191 1471
Fragments arranged by size:
2300 1471 1135 681 538 408 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6943
SmaI CCC'GGG
Cuts at: 1872 4270 1872
Size: 2398 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4118 5047 5948 6210 820
Size: 197 926 2175 929 901 262 1553
Fragments arranged by size:
2175 1553 929 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4240 4734 493
Size: 3747 494 2702
Fragments arranged by size:
3747 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6943
SphI G_CATG'C
Cuts at: 1132 3284 4301 1132
Size: 2152 1017 3774
Fragments arranged by size:
3774 2152 1017
SspI AAT'ATT
Cuts at: 5 52 2739 3333 4867 5
Size: 47 2687 594 1534 2081
Fragments arranged by size:
2687 2081 1534 594 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6943
StyI C'CwwG_G
Cuts at: 513 1065 3144 3849 4185 4382 513
Size: 552 2079 705 336 197 3074
Fragments arranged by size:
3074 2079 705 552 336 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4970 4987 5140 5325 5770 325
Size: 1255 3390 17 153 185 445 1498
Fragments arranged by size:
3390 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2497 3060 3087
Size: 274 80 33 51 1443 520 563 27
Cuts at: 3087 5189 6901 96
Size: 2102 1712 138
Fragments arranged by size:
2102 1712 1443 563 520 274 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3709 3741 6903 6935 3709
Size: 32 3162 32 3717
Fragments arranged by size:
3717 3162 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3112 3565 1316
Size: 1796 453 4694
Fragments arranged by size:
4694 1796 453
XbaI T'CTAG_A
Cuts at: 4279 4279
Size: 6943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2297 2297
Size: 6943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6943
XmaI C'CCGG_G
Cuts at: 1870 4268 1870
Size: 2398 4545
XmnI GAAnn'nnTTC
Cuts at: 5072 5072
Size: 6943
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3039 4751 276
Size: 53 83 186 936 1505 1712 2468
Fragments arranged by size:
2468 1712 1505 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BstXI BstZ17I Bsu36I EcoICRI EcoRV FspAI KasI
NarI NruI NsiI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI