Restriction map for
pFN21ASDA0215
(Circular) (Six-base) MAPSORT of: pFN21ASDA0215.seq Check: 3410 from: 1 to: 7198
pFN21ASDA0215 7198 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3751 3751
Size: 7198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5008 278
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4267 4541 1937
Size: 2330 274 4594
Fragments arranged by size:
4594 2330 274
Acc65I G'GTAC_C
Cuts at: 2266 4519 2266
Size: 2253 4945
AclI AA'CG_TT
Cuts at: 1563 5325 5698 1563
Size: 3762 373 3063
Fragments arranged by size:
3762 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2837 3350 3527 3932 5261 6645 6913
Size: 818 387 513 177 405 1329 1384 268
Cuts at: 6913 1632
Size: 1917
Fragments arranged by size:
1917 1384 1329 818 513 405 387 268
177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7001
AflIII A'CryG_T
Cuts at: 1203 6097 7074 1203
Size: 4894 977 1327
Fragments arranged by size:
4894 1327 977
AgeI A'CCGG_T
Cuts at: 6029 6029
Size: 7198
AhdI GACnn_n'nnGTC
Cuts at: 5927 5927
Size: 7198
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2443 1184
Size: 1259 5939
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 4085 4117 3224
Size: 32 829 32 6305
Fragments arranged by size:
6305 829 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2390 2423 3457 3928 4941 6513 2390
Size: 33 1034 471 1013 1572 3075
Fragments arranged by size:
3075 1572 1034 1013 471 33
ApaLI G'TGCA_C
Cuts at: 5255 6411 5255
Size: 1156 6042
ApoI r'AATT_y
Cuts at: 1493 2118 4507 4733 4885 6994 1493
Size: 625 2389 226 152 2109 1697
Fragments arranged by size:
2389 2109 1697 625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2202 5752 160
Size: 872 1170 3550 1606
Fragments arranged by size:
3550 1606 1170 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
AvaI C'yCGr_G
Cuts at: 1870 1943 4069 4523 1870
Size: 73 2126 454 4545
Fragments arranged by size:
4545 2126 454 73
BamHI G'GATC_C
Cuts at: 2868 4528 4895 2868
Size: 1660 367 5171
Fragments arranged by size:
5171 1660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 3763 4519 5974 618
Size: 325 217 627 479 1497 756 1455 1842
Fragments arranged by size:
1842 1497 1455 756 627 479 325 217
BanII G_rGCy'C
Cuts at: 1391 2815 2848 4247 4300 4517 1391
Size: 1424 33 1399 53 217 4072
Fragments arranged by size:
4072 1424 1399 217 53 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 2825 2981 3068 3681 3963 928
Size: 971 588 338 156 87 613 282 4163
Fragments arranged by size:
4163 971 613 588 338 282 156 87
BbvCI CC'TCA_GC
Cuts at: 2145 2145
Size: 7198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3459 3493 5389 5423 1903
Size: 34 1522 34 1896 34 3678
Fragments arranged by size:
3678 1896 1522 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3810 5090 6300 3810
Size: 1280 1210 4708
Fragments arranged by size:
4708 1280 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7198
BfrBI ATG'CAT
Cuts at: 3442 3442
Size: 7198
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4032 5809 136
Size: 107 122 71 631 605 2360 1777 1525
Fragments arranged by size:
2360 1777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2919 3537 7193 2919
Size: 618 3656 2924
Fragments arranged by size:
3656 2924 618
BlpI GC'TnA_GC
Cuts at: 4615 4615
Size: 7198
Bme1580I G_kGCm'C
Cuts at: 1790 2863 3214 5259 6415 1790
Size: 1073 351 2045 1156 2573
Fragments arranged by size:
2573 2045 1156 1073 351
BmgBI CAC'GTC
Cuts at: 1318 3028 1318
Size: 1710 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3938 4929
Size: 416 53 83 399 24 1514 1007 991
Cuts at: 4929 5887 442
Size: 958 1753
Fragments arranged by size:
1753 1514 1007 991 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3299 1056
Size: 2243 4955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 2631 3251 3479 4049 4196 5858 1239
Size: 1114 278 620 228 570 147 1662 2579
Fragments arranged by size:
2579 1662 1114 620 570 278 228 147
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 7198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 3095 5323 6188 6486 2711
Size: 384 2228 865 298 3423
Fragments arranged by size:
3423 2228 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2649 4169 5861 882
Size: 617 1150 1520 1692 2219
Fragments arranged by size:
2219 1692 1520 1150 617
BsaAI yAC'GTr
Cuts at: 493 1204 4495 4989 493
Size: 711 3291 494 2702
Fragments arranged by size:
3291 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2517 7192 2517
Size: 4675 2523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5005 5387 6022 275
Size: 53 83 186 936 3472 382 635 1451
Fragments arranged by size:
3472 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2154 5631 6029 6303 6450 6861 2154
Size: 3477 398 274 147 411 2491
Fragments arranged by size:
3477 2491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2250 2280 3072 3102 3224
Size: 30 165 30 847 30 792 30 122
Cuts at: 3224 3254 4085 4115 1178
Size: 30 831 30 4261
Fragments arranged by size:
4261 847 831 792 165 122 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3248 3251 1186
Size: 2062 3 5133
Fragments arranged by size:
5133 2062 3
BseYI C'CCAG_C
Cuts at: 3280 6401 6897 3280
Size: 3121 496 3581
Fragments arranged by size:
3581 3121 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3416 1163
Size: 573 1680 4945
Fragments arranged by size:
4945 1680 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4648 5409 6437 1540
Size: 275 186 2647 761 1028 2301
Fragments arranged by size:
2647 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2999 3048 4300 5259 5344 6415 2999
Size: 49 1252 959 85 1071 3782
Fragments arranged by size:
3782 1252 1071 959 85 49
BsmI GAATG_Cn'
Cuts at: 3414 4709 4802 3414
Size: 1295 93 5810
Fragments arranged by size:
5810 1295 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3075 3075
Size: 7198
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2815 2848 2863 2999 3048 3214 4247
Size: 399 1025 33 15 136 49 166 1033
Cuts at: 4247 4300 4517 5259 5344 6415 1391
Size: 53 217 742 85 1071 2174
Fragments arranged by size:
2174 1071 1033 1025 742 399 217 166
136 85 53 49 33 15
BspEI T'CCGG_A
Cuts at: 2154 6861 2154
Size: 4707 2491
BspHI T'CATG_A
Cuts at: 5087 6817 5087
Size: 1730 5468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 3751 4553 844
Size: 1305 1602 802 3489
Fragments arranged by size:
3489 1602 1305 802
BsrBI CCG'CTC
Cuts at: 1394 1823 5085 1394
Size: 429 3262 3507
Fragments arranged by size:
3507 3262 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 2789 2883 5693 5867 65
Size: 1183 1306 235 94 2810 174 1396
Fragments arranged by size:
2810 1396 1306 1183 235 174 94
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 5842 6029 1700
Size: 110 830 3202 187 2869
Fragments arranged by size:
3202 2869 830 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7198
BssSI C'ACGA_G
Cuts at: 5258 6270 5258
Size: 1012 6186
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2964 794
Size: 2170 5028
BstBI TT'CG_AA
Cuts at: 2007 6002 2007
Size: 3995 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7198
BstXI CCAn_nnnn'nTGG
Cuts at: 4027 4027
Size: 7198
BstYI r'GATC_y
Cuts at: 1972 2868 2919 3537 4528 4895 5280 5297 6749
Size: 896 51 618 991 367 385 17 1452
Cuts at: 6749 6835 6847 7193 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 991 896 618 385 367 346
86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 3768 3768
Size: 7198
BtgI C'CryG_G
Cuts at: 513 1065 3961 4444 513
Size: 552 2896 483 3267
Fragments arranged by size:
3267 2896 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6459
Fragments arranged by size:
6459 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2362 3015 4714 5508 5528 2056
Size: 306 653 1699 794 20 3726
Fragments arranged by size:
3726 1699 794 653 306 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7163
DraI TTT'AAA
Cuts at: 4171 4502 4171
Size: 331 6867
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6205 809
Size: 507 4889 1802
Fragments arranged by size:
4889 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3270 3840 4645
Size: 54 1425 50 275 18 1440 570 805
Cuts at: 4645 5534 7163 8
Size: 889 1629 43
Fragments arranged by size:
1629 1440 1425 889 805 570 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4645 1537
Size: 275 2833 4090
Fragments arranged by size:
4090 2833 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5128 1353
Size: 3775 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5785 6159 6305 387
Size: 1064 138 4196 374 146 1280
Fragments arranged by size:
4196 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4298 4298
Size: 7198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2450 2631 2837 3251 3350 3479
Size: 393 721 97 181 206 414 99 129
Cuts at: 3479 3527 3932 4049 4196 5261 5858 6645 6913
Size: 48 405 117 147 1065 597 787 268
Cuts at: 6913 1239
Size: 1524
Fragments arranged by size:
1524 1065 787 721 597 414 405 393
268 206 181 147 129 117 99 97
48
EcoNI CCTnn'n_nnAGG
Cuts at: 3074 3074
Size: 7198
EcoO109I rG'GnC_Cy
Cuts at: 2161 2731 3454 4164 4207 2161
Size: 570 723 710 43 5152
Fragments arranged by size:
5152 723 710 570 43
EcoRI G'AATT_C
Cuts at: 4507 4885 4507
Size: 378 6820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3575 3575
Size: 7198
FspI TGC'GCA
Cuts at: 5704 5704
Size: 7198
HaeII r_GCGC'y
Cuts at: 1678 6345 1678
Size: 4667 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3716 3748 4260 4292 5839
Size: 32 135 32 2144 32 512 32 1547
Cuts at: 5839 5871 5913 5945 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2144 1547 512 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4542 4788 669
Size: 988 281 2604 246 3079
Fragments arranged by size:
3079 2604 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3218 748
Size: 2470 4728
HpaI GTT'AAC
Cuts at: 4788 4788
Size: 7198
KpnI G_GTAC'C
Cuts at: 2270 4523 2270
Size: 2253 4945
MfeI C'AATT_G
Cuts at: 4797 4797
Size: 7198
MluI A'CGCG_T
Cuts at: 7074 7074
Size: 7198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2368 6312 6496 1305
Size: 1063 3944 184 2007
Fragments arranged by size:
3944 2007 1063 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3842 10
Size: 54 1425 343 2010 3366
Fragments arranged by size:
3366 2010 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 3425 3761 5156 5515 5674 518
Size: 648 1393 866 336 1395 359 159 2042
Fragments arranged by size:
2042 1395 1393 866 648 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 2745 2856 4038 4614 5291 6439 6684 1606
Size: 1139 111 1182 576 677 1148 245 2120
Fragments arranged by size:
2120 1182 1148 1139 677 576 245 111
NcoI C'CATG_G
Cuts at: 513 1065 3961 4444 513
Size: 552 2896 483 3267
Fragments arranged by size:
3267 2896 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7198
NheI G'CTAG_C
Cuts at: 1052 3295 1052
Size: 2243 4955
NotI GC'GGCC_GC
Cuts at: 4645 4645
Size: 7198
NsiI A_TGCA'T
Cuts at: 3444 3444
Size: 7198
NspI r_CATG'y
Cuts at: 1132 1229 3760 4556 6101 1132
Size: 97 2531 796 1545 2229
Fragments arranged by size:
2531 2229 1545 796 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7198
PciI A'CATG_T
Cuts at: 6097 6097
Size: 7198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2943 3457 4188 1108
Size: 1835 514 731 4118
Fragments arranged by size:
4118 1835 731 514
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 7198
PmeI GTTT'AAAC
Cuts at: 4502 4502
Size: 7198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4085 4117 5267 5299 6780 6812 3224
Size: 32 829 32 1150 32 1481 32 3610
Fragments arranged by size:
3610 1481 1150 829 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2731 3454 4164 4207 2161
Size: 570 723 710 43 5152
Fragments arranged by size:
5152 723 710 570 43
PshAI GACnn'nnGTC
Cuts at: 1613 2067 1613
Size: 454 6744
PsiI TTA'TAA
Cuts at: 4768 4768
Size: 7198
PspXI vC'TCGA_Gb
Cuts at: 4069 4069
Size: 7198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2316 2348 2316
Size: 32 7166
PstI C_TGCA'G
Cuts at: 830 4291 4550 830
Size: 3461 259 3478
Fragments arranged by size:
3478 3461 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7198
PvuII CAG'CTG
Cuts at: 2745 2856 4038 2745
Size: 111 1182 5905
Fragments arranged by size:
5905 1182 111
SacI G_AGCT'C
Cuts at: 4300 4300
Size: 7198
SalI G'TCGA_C
Cuts at: 1936 4540 1936
Size: 2604 4594
SbfI CC_TGCA'GG
Cuts at: 4550 4550
Size: 7198
ScaI AGT'ACT
Cuts at: 5446 7158 5446
Size: 1712 5486
SfcI C'TryA_G
Cuts at: 826 1045 4146 4287 4546 5681 6362 6553 826
Size: 219 3101 141 259 1135 681 191 1471
Fragments arranged by size:
3101 1471 1135 681 259 219 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7198
SmaI CCC'GGG
Cuts at: 1872 4525 1872
Size: 2653 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3110 4069 5302 6203 6465
Size: 197 926 783 384 959 1233 901 262
Cuts at: 6465 820
Size: 1553
Fragments arranged by size:
1553 1233 959 926 901 783 384 262
197
SnaBI TAC'GTA
Cuts at: 493 4495 4989 493
Size: 4002 494 2702
Fragments arranged by size:
4002 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7198
SphI G_CATG'C
Cuts at: 1132 4556 1132
Size: 3424 3774
SspI AAT'ATT
Cuts at: 5 52 5122 5
Size: 47 5070 2081
Fragments arranged by size:
5070 2081 47
StuI AGG'CCT
Cuts at: 1517 2038 2711 1517
Size: 521 673 6004
Fragments arranged by size:
6004 673 521
StyI C'CwwG_G
Cuts at: 513 1065 3249 3961 4248 4444 4637 513
Size: 552 2184 712 287 196 193 3074
Fragments arranged by size:
3074 2184 712 552 287 196 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2950 4070 4180 5225 5242 5395 5580
Size: 1255 1370 1120 110 1045 17 153 185
Cuts at: 5580 6025 325
Size: 445 1498
Fragments arranged by size:
1498 1370 1255 1120 1045 445 185 153
110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5444 7156 96
Size: 274 80 33 51 1443 3467 1712 138
Fragments arranged by size:
3467 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3072 3104 4199 4231 7158 7190 3072
Size: 32 1095 32 2927 32 3080
Fragments arranged by size:
3080 2927 1095 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7198
XbaI T'CTAG_A
Cuts at: 4430 4534 4430
Size: 104 7094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2742 2940 3298 2742
Size: 198 358 6642
Fragments arranged by size:
6642 358 198
XhoI C'TCGA_G
Cuts at: 1943 4069 1943
Size: 2126 5072
XmaI C'CCGG_G
Cuts at: 1870 4523 1870
Size: 2653 4545
XmnI GAAnn'nnTTC
Cuts at: 3414 5327 3414
Size: 1913 5285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5006 276
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI