Restriction map for
pFN21ASDA0214
(Circular) (Six-base) MAPSORT of: pFN21ASDA0214.seq Check: 3187 from: 1 to: 7000
pFN21ASDA0214 7000 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3298 3298
Size: 7000
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2256 4137 4810 278
Size: 53 83 186 936 720 1881 673 2468
Fragments arranged by size:
2468 1881 936 720 673 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 4343 1937
Size: 1377 1029 4594
Fragments arranged by size:
4594 1377 1029
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3006 4187 5063 6447 6715 1632
Size: 983 391 1181 876 1384 268 1917
Fragments arranged by size:
1917 1384 1181 983 876 391 268
AflII C'TTAA_G
Cuts at: 820 1017 2108 820
Size: 197 1091 5712
Fragments arranged by size:
5712 1091 197
AflIII A'CryG_T
Cuts at: 1203 2199 3453 4260 5899 6876 1203
Size: 996 1254 807 1639 977 1327
Fragments arranged by size:
1639 1327 1254 996 977 807
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 3580 1166
Size: 2414 4586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2559 4179 1184
Size: 1375 1620 4005
Fragments arranged by size:
4005 1620 1375
AlwNI CAG_nnn'CTG
Cuts at: 2248 4096 4279 4743 6315 2248
Size: 1848 183 464 1572 2933
Fragments arranged by size:
2933 1848 1572 464 183
ApaI G_GGCC'C
Cuts at: 3698 3698
Size: 7000
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 3040 3227 4309 4535 4687 6796 1493
Size: 1547 187 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 1082 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 3171 4140 4325 1870
Size: 73 879 349 969 185 4545
Fragments arranged by size:
4545 969 879 349 185 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4259 4292 4259
Size: 33 6967
BamHI G'GATC_C
Cuts at: 4330 4697 4330
Size: 367 6633
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3339 4321 5776 618
Size: 325 217 627 1552 982 1455 1842
Fragments arranged by size:
1842 1552 1455 982 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2474 2823 3252 3698 3800 3942 4257 4319
Size: 1083 349 429 446 102 142 315 62
Cuts at: 4319 1391
Size: 4072
Fragments arranged by size:
4072 1083 446 429 349 315 142 102
62
BbeI G_GCGC'C
Cuts at: 3343 3343
Size: 7000
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 3092 3645 928
Size: 971 303 890 553 4283
Fragments arranged by size:
4283 971 890 553 303
BbvCI CC'TCA_GC
Cuts at: 2088 2088
Size: 7000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 5191 5225 1903
Size: 34 98 34 3122 34 3678
Fragments arranged by size:
3678 3122 98 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4892 6102 4892
Size: 1210 5790
BclI T'GATC_A
Cuts at: 1548 2358 1548
Size: 810 6190
BfrBI ATG'CAT
Cuts at: 2099 2807 2099
Size: 708 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5611 136
Size: 107 122 71 631 605 3939 1525
Fragments arranged by size:
3939 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6995 6995
Size: 7000
BlpI GC'TnA_GC
Cuts at: 4417 4417
Size: 7000
Bme1580I G_kGCm'C
Cuts at: 1790 2438 2588 3698 5061 6217 1790
Size: 648 150 1110 1363 1156 2573
Fragments arranged by size:
2573 1363 1156 1110 648 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3067 3452 4731
Size: 416 53 83 399 24 1650 385 1279
Cuts at: 4731 5689 442
Size: 958 1753
Fragments arranged by size:
1753 1650 1279 958 416 399 385 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 3085 2782
Size: 303 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3266 3372 4172 5660 1239
Size: 2027 106 800 1488 2579
Fragments arranged by size:
2579 2027 1488 800 106
Bpu10I CC'TnA_GC
Cuts at: 2088 2940 4223 2088
Size: 852 1283 4865
Fragments arranged by size:
4865 1283 852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3243 3383 5125 5990 6288 3243
Size: 140 1742 865 298 3955
Fragments arranged by size:
3955 1742 865 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5663 882
Size: 617 4164 2219
Fragments arranged by size:
4164 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2202 4297 4791 493
Size: 711 998 2095 494 2702
Fragments arranged by size:
2702 2095 998 711 494
BsaBI GATnn'nnATC
Cuts at: 6994 6994
Size: 7000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 3340 3761 4134
Size: 53 83 186 936 720 1087 421 373
Cuts at: 4134 4807 5189 5824 275
Size: 673 382 635 1451
Fragments arranged by size:
1451 1087 936 720 673 635 421 382
373 186 83 53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3452 3482 3879 3909 4049
Size: 30 165 30 2049 30 397 30 140
Cuts at: 4049 4079 1178
Size: 30 4099
Fragments arranged by size:
4099 2049 397 165 140 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2500 2849 3064 3074 3344 3827 3903 3941
Size: 1314 349 215 10 270 483 76 38
Cuts at: 3941 1186
Size: 4245
Fragments arranged by size:
4245 1314 483 349 270 215 76 38
10
BseYI C'CCAG_C
Cuts at: 2158 2799 2887 3773 4286 6203 6699 2158
Size: 641 88 886 513 1917 496 2459
Fragments arranged by size:
2459 1917 886 641 513 496 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3143 3404 1163
Size: 573 1407 261 4759
Fragments arranged by size:
4759 1407 573 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4450 5211 6239 1540
Size: 275 186 2449 761 1028 2301
Fragments arranged by size:
2449 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2354 2952 3252 4257 5061 5146 6217 2354
Size: 598 300 1005 804 85 1071 3137
Fragments arranged by size:
3137 1071 1005 804 598 300 85
BsmI GAATG_Cn'
Cuts at: 2101 2215 3873 4511 4604 2101
Size: 114 1658 638 93 4497
Fragments arranged by size:
4497 1658 638 114 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2768 2768
Size: 7000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2438 2474 2588 2823 2952 3252
Size: 399 564 84 36 114 235 129 300
Cuts at: 3252 3698 3800 3942 4257 4319 5061 5146 6217
Size: 446 102 142 315 62 742 85 1071
Cuts at: 6217 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 564 446 399 315 300
235 142 129 114 102 85 84 62
36
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3172 3298 4287 4355 844
Size: 2328 126 989 68 3489
Fragments arranged by size:
3489 2328 989 126 68
BsrBI CCG'CTC
Cuts at: 1394 1823 3211 4887 1394
Size: 429 1388 1676 3507
Fragments arranged by size:
3507 1676 1388 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2347 3327 3921 5495 5669 65
Size: 1183 1099 980 594 1574 174 1396
Fragments arranged by size:
1574 1396 1183 1099 980 594 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2546 3836 4235 5644 5831 1700
Size: 110 736 1290 399 1409 187 2869
Fragments arranged by size:
2869 1409 1290 736 399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 5060 6072 5060
Size: 1012 5988
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3499 794
Size: 2705 4295
BstBI TT'CG_AA
Cuts at: 2007 5804 2007
Size: 3797 3203
BstEII G'GTnAC_C
Cuts at: 1184 2261 3832 1184
Size: 1077 1571 4352
Fragments arranged by size:
4352 1571 1077
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 2875 2647
Size: 228 6772
BstYI r'GATC_y
Cuts at: 1972 4330 4697 5082 5099 6551 6637 6649 6995
Size: 2358 367 385 17 1452 86 12 346
Cuts at: 6995 1972
Size: 1977
Fragments arranged by size:
2358 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3471 3828 3867 513
Size: 552 2406 357 39 3646
Fragments arranged by size:
3646 2406 552 357 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 3087 3785 3912 4516 5310 5330 2121
Size: 966 698 127 604 794 20 3791
Fragments arranged by size:
3791 966 794 698 604 127 20
ClaI AT'CG_AT
Cuts at: 1554 2902 1554
Size: 1348 5652
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2173 2208 529
Size: 35 1609 35 5321
Fragments arranged by size:
5321 1609 35 35
DraI TTT'AAA
Cuts at: 4304 4304
Size: 7000
DraIII CAC_nnn'GTG
Cuts at: 2752 2752
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3569 6007 809
Size: 507 2253 2438 1802
Fragments arranged by size:
2438 2253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 2401 2443
Size: 54 1425 50 275 18 499 72 42
Cuts at: 2443 2700 2771 3324 3474 4447 5336 6965 8
Size: 257 71 553 150 973 889 1629 43
Fragments arranged by size:
1629 1425 973 889 553 499 275 257
150 72 71 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2342 3201 4930 1353
Size: 686 303 859 1729 3423
Fragments arranged by size:
3423 1729 859 686 303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5587 5961 6107 387
Size: 1064 138 3998 374 146 1280
Fragments arranged by size:
3998 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3250 4255 3250
Size: 1005 5995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 3006 3266 3372 4172 4187 5063
Size: 393 983 391 260 106 800 15 876
Cuts at: 5063 5660 6447 6715 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 983 876 800 787 597 393 391
268 260 106 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2525 2988 4154 2465
Size: 60 463 1166 5311
Fragments arranged by size:
5311 1166 463 60
EcoO109I rG'GnC_Cy
Cuts at: 2163 3695 3861 3999 4104 2163
Size: 1532 166 138 105 5059
Fragments arranged by size:
5059 1532 166 138 105
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 7000
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 2184 3000 3343 4012 6147 1678
Size: 506 816 343 669 2135 2531
Fragments arranged by size:
2531 2135 816 669 506 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2746 2778 3452 3484 4096
Size: 32 135 32 1174 32 674 32 612
Cuts at: 4096 4128 5641 5673 5715 5747 1373
Size: 32 1513 32 42 32 2626
Fragments arranged by size:
2626 1513 1174 674 612 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 4132 4344 4590 669
Size: 988 281 1377 817 212 246 3079
Fragments arranged by size:
3079 1377 988 817 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KasI G'GCGC_C
Cuts at: 3339 3339
Size: 7000
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 2413 4599 2413
Size: 2186 4814
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3865 4092 4224 6114 6298 1305
Size: 2560 227 132 1890 184 2007
Fragments arranged by size:
2560 2007 1890 227 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2403 2445 2702 10
Size: 54 1425 343 571 42 257 4308
Fragments arranged by size:
4308 1425 571 343 257 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2720 3580 3677 4958 5317 5476
Size: 648 1149 405 860 97 1281 359 159
Cuts at: 5476 518
Size: 2042
Fragments arranged by size:
2042 1281 1149 860 648 405 359 159
97
MspA1I CmG'CkG
Cuts at: 1606 2158 2509 2655 2799 3244 3465 4416 5093
Size: 552 351 146 144 445 221 951 677
Cuts at: 5093 6241 6486 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 552 445 351 245
221 146 144
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 7000
NarI GG'CG_CC
Cuts at: 3340 3340
Size: 7000
NcoI C'CATG_G
Cuts at: 513 1065 3828 3867 513
Size: 552 2763 39 3646
Fragments arranged by size:
3646 2763 552 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NsiI A_TGCA'T
Cuts at: 2101 2809 2101
Size: 708 6292
NspI r_CATG'y
Cuts at: 1132 1229 2983 3457 3568 3781 4028 4264 4358
Size: 97 1754 474 111 213 247 236 94
Cuts at: 4358 5903 1132
Size: 1545 2229
Fragments arranged by size:
2229 1754 1545 474 247 236 213 111
97 94
PasI CC'CwG_GG
Cuts at: 1348 2974 1348
Size: 1626 5374
PciI A'CATG_T
Cuts at: 3453 4260 5899 3453
Size: 807 1639 4554
Fragments arranged by size:
4554 1639 807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 4018 1108
Size: 1265 1645 4090
Fragments arranged by size:
4090 1645 1265
PfoI T'CCnGG_A
Cuts at: 2172 3633 3857 2172
Size: 1461 224 5315
Fragments arranged by size:
5315 1461 224
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3861 4104 3861
Size: 243 6757
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 3408 4570 3408
Size: 1162 5838
PspOMI G'GGCC_C
Cuts at: 3694 3694
Size: 7000
PstI C_TGCA'G
Cuts at: 830 3082 3495 4044 4076 4284 4352 830
Size: 2252 413 549 32 208 68 3478
Fragments arranged by size:
3478 2252 549 413 208 68 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 2158 2509 2655 3244 2158
Size: 351 146 589 5914
Fragments arranged by size:
5914 589 351 146
RsrII CG'GwC_CG
Cuts at: 3109 3109
Size: 7000
SacI G_AGCT'C
Cuts at: 3252 4257 3252
Size: 1005 5995
SalI G'TCGA_C
Cuts at: 1936 3313 4342 1936
Size: 1377 1029 4594
Fragments arranged by size:
4594 1377 1029
SapI GCTCTTCn'nnn_
Cuts at: 2039 2342 3201 2039
Size: 303 859 5838
Fragments arranged by size:
5838 859 303
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 5248 6960 5248
Size: 1712 5288
SexAI A'CCwGG_T
Cuts at: 3735 3735
Size: 7000
SfcI C'TryA_G
Cuts at: 826 1045 3078 3491 4040 4072 4280 4348 5483
Size: 219 2033 413 549 32 208 68 1135
Cuts at: 5483 6164 6355 826
Size: 681 191 1471
Fragments arranged by size:
2033 1471 1135 681 549 413 219 208
191 68 32
SfoI GGC'GCC
Cuts at: 3341 3341
Size: 7000
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 4142 4327 1872
Size: 2270 185 4545
Fragments arranged by size:
4545 2270 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 3258 3398 5104 6005 6267
Size: 197 926 165 1150 140 1706 901 262
Cuts at: 6267 820
Size: 1553
Fragments arranged by size:
1706 1553 1150 926 901 262 197 165
140
SnaBI TAC'GTA
Cuts at: 493 4297 4791 493
Size: 3804 494 2702
Fragments arranged by size:
3804 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 2983 3781 4028 4358 1132
Size: 1851 798 247 330 3774
Fragments arranged by size:
3774 1851 798 330 247
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 2453 1517
Size: 936 6064
StyI C'CwwG_G
Cuts at: 513 1065 3828 3867 3984 3990 4439 513
Size: 552 2763 39 117 6 449 3074
Fragments arranged by size:
3074 2763 552 449 117 39 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3234 3821 5027 5044 5197 5382 5827
Size: 1255 1654 587 1206 17 153 185 445
Cuts at: 5827 325
Size: 1498
Fragments arranged by size:
1654 1498 1255 1206 587 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2826 3349 5246
Size: 274 80 33 51 1443 849 523 1897
Cuts at: 5246 6958 96
Size: 1712 138
Fragments arranged by size:
1897 1712 1443 849 523 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3879 3911 6960 6992 3879
Size: 32 3049 32 3887
Fragments arranged by size:
3887 3049 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3444 3737 1316
Size: 2128 293 4579
Fragments arranged by size:
4579 2128 293
XbaI T'CTAG_A
Cuts at: 4336 4336
Size: 7000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 3865 2875
Size: 990 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 4140 4325 1870
Size: 2270 185 4545
Fragments arranged by size:
4545 2270 185
XmnI GAAnn'nnTTC
Cuts at: 2244 3012 3686 5129 2244
Size: 768 674 1443 4115
Fragments arranged by size:
4115 1443 768 674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2254 4135 4808 276
Size: 53 83 186 936 720 1881 673 2468
Fragments arranged by size:
2468 1881 936 720 673 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BsiWI BstZ17I Bsu36I EcoRV
FseI FspAI NruI PacI PmlI PspXI PsrI SacII
SanDI SfiI SrfI SwaI