Restriction map for
pFN21ASDA0212
(Circular) (Six-base) MAPSORT of: pFN21ASDA0212.seq Check: 4760 from: 1 to: 6700
pFN21ASDA0212 6700 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3349 3349
Size: 6700
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2974 4043 1937
Size: 1037 1069 4594
Fragments arranged by size:
4594 1069 1037
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3843 4763 6147 6415 1632
Size: 2211 920 1384 268 1917
Fragments arranged by size:
2211 1917 1384 920 268
AfeI AGC'GCT
Cuts at: 2037 2129 2372 2037
Size: 92 243 6365
Fragments arranged by size:
6365 243 92
AflII C'TTAA_G
Cuts at: 820 1017 3229 820
Size: 197 2212 4291
Fragments arranged by size:
4291 2212 197
AflIII A'CryG_T
Cuts at: 1203 3294 3747 5599 6576 1203
Size: 2091 453 1852 977 1327
Fragments arranged by size:
2091 1852 1327 977 453
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 5429 5429
Size: 6700
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 3977 1184
Size: 1234 1559 3907
Fragments arranged by size:
3907 1559 1234
AlwNI CAG_nnn'CTG
Cuts at: 2158 3364 3583 4443 6015 2158
Size: 1206 219 860 1572 2843
Fragments arranged by size:
2843 1572 1206 860 219
ApaI G_GGCC'C
Cuts at: 2109 2199 2517 2109
Size: 90 318 6292
Fragments arranged by size:
6292 318 90
ApaLI G'TGCA_C
Cuts at: 3857 4757 5913 3857
Size: 900 1156 4644
Fragments arranged by size:
4644 1156 900
ApoI r'AATT_y
Cuts at: 1493 2614 2957 3812 4009 4235 4387 6496 1493
Size: 1121 343 855 197 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 855 343 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 2191 2877 4025 1870
Size: 73 248 686 1148 4545
Fragments arranged by size:
4545 1148 686 248 73
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2381 3119 3431 4021 5476
Size: 325 217 627 594 738 312 590 1455
Cuts at: 5476 618
Size: 1842
Fragments arranged by size:
1842 1455 738 627 594 590 325 312
217
BanII G_rGCy'C
Cuts at: 1391 2109 2168 2199 2270 2487 2517 4019 1391
Size: 718 59 31 71 217 30 1502 4072
Fragments arranged by size:
4072 1502 718 217 71 59 31 30
BbeI G_GCGC'C
Cuts at: 2385 2385
Size: 6700
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 3189 3657 3720 928
Size: 971 187 1103 468 63 3908
Fragments arranged by size:
3908 1103 971 468 187 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4891 4925 1903
Size: 34 2954 34 3678
Fragments arranged by size:
3678 2954 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2230 2587 4592 5802 2230
Size: 357 2005 1210 3128
Fragments arranged by size:
3128 2005 1210 357
BclI T'GATC_A
Cuts at: 1548 2649 1548
Size: 1101 5599
BfrBI ATG'CAT
Cuts at: 2588 2588
Size: 6700
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 2233 5311
Size: 107 122 71 631 605 500 61 3078
Cuts at: 5311 136
Size: 1525
Fragments arranged by size:
3078 1525 631 605 500 122 107 71
61
BglII A'GATC_T
Cuts at: 6695 6695
Size: 6700
BlpI GC'TnA_GC
Cuts at: 2138 4117 2138
Size: 1979 4721
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2199 2407 2517 3436 3861 4761 5917
Size: 319 90 208 110 919 425 900 1156
Cuts at: 5917 1790
Size: 2573
Fragments arranged by size:
2573 1156 919 900 425 319 208 110
90
BmgBI CAC'GTC
Cuts at: 1318 3631 1318
Size: 2313 4387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3103 4431
Size: 416 53 83 399 24 912 774 1328
Cuts at: 4431 5389 442
Size: 958 1753
Fragments arranged by size:
1753 1328 958 912 774 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3880 5360 1239
Size: 2641 1480 2579
Fragments arranged by size:
2641 2579 1480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4825 5690 5988 4825
Size: 865 298 5537
Fragments arranged by size:
5537 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3261 3487 3674 5363 882
Size: 617 1762 226 187 1689 2219
Fragments arranged by size:
2219 1762 1689 617 226 187
BsaAI yAC'GTr
Cuts at: 493 1204 2329 3997 4491 493
Size: 711 1125 1668 494 2702
Fragments arranged by size:
2702 1668 1125 711 494
BsaBI GATnn'nnATC
Cuts at: 3786 6694 3786
Size: 2908 3792
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2287 2382 4507 4889
Size: 53 83 186 936 754 95 2125 382
Cuts at: 4889 5524 275
Size: 635 1451
Fragments arranged by size:
2125 1451 936 754 635 382 186 95
83 53
BsaWI w'CCGG_w
Cuts at: 3039 3654 5133 5531 5805 5952 6363 3039
Size: 615 1479 398 274 147 411 3376
Fragments arranged by size:
3376 1479 615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3884 3914 1178
Size: 30 165 30 2481 30 3964
Fragments arranged by size:
3964 2481 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2176 2825 3512 3943 1186
Size: 862 128 649 687 431 3943
Fragments arranged by size:
3943 862 687 649 431 128
BseYI C'CCAG_C
Cuts at: 2101 2108 2311 2332 5903 6399 2101
Size: 7 203 21 3571 496 2402
Fragments arranged by size:
3571 2402 496 203 21 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2341 2509 2521 4150 4911 5939
Size: 275 186 340 168 12 1629 761 1028
Cuts at: 5939 1540
Size: 2301
Fragments arranged by size:
2301 1629 1028 761 340 275 186 168
12
BsiHKAI G_wGCw'C
Cuts at: 2058 2487 3861 4761 4846 5917 2058
Size: 429 1374 900 85 1071 2841
Fragments arranged by size:
2841 1374 1071 900 429 85
BsmI GAATG_Cn'
Cuts at: 4211 4304 4211
Size: 93 6607
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2109 2168 2199 2270 2407 2487
Size: 399 268 51 59 31 71 137 80
Cuts at: 2487 2517 3436 3861 4019 4761 4846 5917 1391
Size: 30 919 425 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 425 399 268 158
137 85 80 71 59 51 31 30
BspEI T'CCGG_A
Cuts at: 3039 6363 3039
Size: 3324 3376
BspHI T'CATG_A
Cuts at: 3874 4589 6319 3874
Size: 715 1730 4255
Fragments arranged by size:
4255 1730 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3349 4055 844
Size: 2505 706 3489
Fragments arranged by size:
3489 2505 706
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 4587 1394
Size: 429 314 2450 3507
Fragments arranged by size:
3507 2450 429 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 2033 3052 3073 3259 5195 5369 65
Size: 1183 785 1019 21 186 1936 174 1396
Fragments arranged by size:
1936 1396 1183 1019 785 186 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 2939 5344 5531 1700
Size: 110 395 734 2405 187 2869
Fragments arranged by size:
2869 2405 734 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 4760 5772 4760
Size: 1012 5688
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6700
BstBI TT'CG_AA
Cuts at: 2007 5504 2007
Size: 3497 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstXI CCAn_nnnn'nTGG
Cuts at: 2319 3098 3983 2319
Size: 779 885 5036
Fragments arranged by size:
5036 885 779
BstYI r'GATC_y
Cuts at: 1972 4030 4397 4782 4799 6251 6337 6349 6695
Size: 2058 367 385 17 1452 86 12 346
Cuts at: 6695 1972
Size: 1977
Fragments arranged by size:
2058 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2074 2199 2282 2347 2503 2509 2521
Size: 552 1009 125 83 65 156 6 12
Cuts at: 2521 3803 513
Size: 1282 3410
Fragments arranged by size:
3410 1282 1009 552 156 125 83 65
12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5961
Fragments arranged by size:
5961 683 56
BtsI GCAGTG_nn'
Cuts at: 2497 3024 4216 5010 5030 2497
Size: 527 1192 794 20 4167
Fragments arranged by size:
4167 1192 794 527 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5707 809
Size: 507 4391 1802
Fragments arranged by size:
4391 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 2506 3006
Size: 54 1425 50 275 18 508 168 500
Cuts at: 3006 4147 5036 6665 8
Size: 1141 889 1629 43
Fragments arranged by size:
1629 1425 1141 889 508 500 275 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2338 2506 4147 1537
Size: 275 526 168 1641 4090
Fragments arranged by size:
4090 1641 526 275 168
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 3485 3819 4630 1353
Size: 1876 256 334 811 3423
Fragments arranged by size:
3423 1876 811 334 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5287 5661 5807 387
Size: 1064 138 3698 374 146 1280
Fragments arranged by size:
3698 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2485 2485
Size: 6700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3843 3880 4763 5360 6147 6415 1239
Size: 393 2211 37 883 597 787 268 1524
Fragments arranged by size:
2211 1524 883 787 597 393 268 37
EcoNI CCTnn'n_nnAGG
Cuts at: 3527 3527
Size: 6700
EcoO109I rG'GnC_Cy
Cuts at: 2105 2195 2527 2710 3466 2105
Size: 90 332 183 756 5339
Fragments arranged by size:
5339 756 332 183 90
EcoRI G'AATT_C
Cuts at: 3812 4009 4387 3812
Size: 197 378 6125
Fragments arranged by size:
6125 378 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 3562 3832 2779
Size: 783 270 5647
Fragments arranged by size:
5647 783 270
FseI GG_CCGG'CC
Cuts at: 2209 2209
Size: 6700
FspI TGC'GCA
Cuts at: 5206 5206
Size: 6700
HaeII r_GCGC'y
Cuts at: 1678 2039 2131 2374 2385 5847 1678
Size: 361 92 243 11 3462 2531
Fragments arranged by size:
3462 2531 361 243 92 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2857 2889 2956
Size: 32 135 32 453 32 800 32 67
Cuts at: 2956 2988 3316 3348 5341 5373 5415 5447 1373
Size: 32 328 32 1993 32 42 32 2626
Fragments arranged by size:
2626 1993 800 453 328 135 67 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2975 3286 4044 4290 669
Size: 988 281 1037 311 758 246 3079
Fragments arranged by size:
3079 1037 988 758 311 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6700
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KasI G'GCGC_C
Cuts at: 2381 2381
Size: 6700
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 6576 6576
Size: 6700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3079 3927 5814 5998 1305
Size: 1774 848 1887 184 2007
Fragments arranged by size:
2007 1887 1774 848 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 10
Size: 54 1425 343 1176 3702
Fragments arranged by size:
3702 1425 1176 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2998 3959 4658 5017 5176 518
Size: 648 1832 961 699 359 159 2042
Fragments arranged by size:
2042 1832 961 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2284 2349 2379 2398 2505 2523 2933 3310
Size: 678 65 30 19 107 18 410 377
Cuts at: 3310 3460 4116 4793 5941 6186 1606
Size: 150 656 677 1148 245 2120
Fragments arranged by size:
2120 1148 678 677 656 410 377 245
150 107 65 30 19 18
NaeI GCC'GGC
Cuts at: 2207 2207
Size: 6700
NarI GG'CG_CC
Cuts at: 2382 2382
Size: 6700
NcoI C'CATG_G
Cuts at: 513 1065 2074 3803 513
Size: 552 1009 1729 3410
Fragments arranged by size:
3410 1729 1009 552
NdeI CA'TA_TG
Cuts at: 387 3416 387
Size: 3029 3671
NgoMIV G'CCGG_C
Cuts at: 2205 2205
Size: 6700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NsiI A_TGCA'T
Cuts at: 2590 2590
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 2434 2928 3298 3751 3964 4058 5603
Size: 97 1205 494 370 453 213 94 1545
Cuts at: 5603 1132
Size: 2229
Fragments arranged by size:
2229 1545 1205 494 453 370 213 97
94
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6700
PciI A'CATG_T
Cuts at: 3294 3747 5599 3294
Size: 453 1852 4395
Fragments arranged by size:
4395 1852 453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 2821 1108
Size: 920 793 4987
Fragments arranged by size:
4987 920 793
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 6282 6314 4769
Size: 32 1481 32 5155
Fragments arranged by size:
5155 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2710 2527
Size: 183 6517
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6700
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PspOMI G'GGCC_C
Cuts at: 2105 2195 2513 2105
Size: 90 318 6292
Fragments arranged by size:
6292 318 90
PstI C_TGCA'G
Cuts at: 830 2241 3339 3360 3465 3579 4052 830
Size: 1411 1098 21 105 114 473 3478
Fragments arranged by size:
3478 1411 1098 473 114 105 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 2398 3310 3460 2398
Size: 912 150 5638
Fragments arranged by size:
5638 912 150
SacI G_AGCT'C
Cuts at: 2487 2487
Size: 6700
SacII CC_GC'GG
Cuts at: 2285 2350 2506 2524 2285
Size: 65 156 18 6461
Fragments arranged by size:
6461 156 65 18
SalI G'TCGA_C
Cuts at: 1936 2973 4042 1936
Size: 1037 1069 4594
Fragments arranged by size:
4594 1069 1037
SanDI GG'GwC_CC
Cuts at: 2527 2527
Size: 6700
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 4948 6660 4948
Size: 1712 4988
SfcI C'TryA_G
Cuts at: 826 1045 2237 2374 3335 3356 3461 3575 4048
Size: 219 1192 137 961 21 105 114 473
Cuts at: 4048 5183 5864 6055 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 961 681 473 219 191
137 114 105 21
SfoI GGC'GCC
Cuts at: 2383 2383
Size: 6700
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3229 4804 5705 5967 820
Size: 197 926 1286 1575 901 262 1553
Fragments arranged by size:
1575 1553 1286 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3997 4491 493
Size: 3504 494 2702
Fragments arranged by size:
3504 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 3692 4624 5
Size: 47 3640 932 2081
Fragments arranged by size:
3640 2081 932 47
StuI AGG'CCT
Cuts at: 1517 3341 1517
Size: 1824 4876
StyI C'CwwG_G
Cuts at: 513 1065 2074 3803 3841 4139 513
Size: 552 1009 1729 38 298 3074
Fragments arranged by size:
3074 1729 1009 552 298 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2992 3107 4727 4744 4897 5082 5527
Size: 1255 1412 115 1620 17 153 185 445
Cuts at: 5527 325
Size: 1498
Fragments arranged by size:
1620 1498 1412 1255 445 185 153 115
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2554 3591 3724
Size: 274 80 33 51 1443 577 1037 133
Cuts at: 3724 4946 6658 96
Size: 1222 1712 138
Fragments arranged by size:
1712 1443 1222 1037 577 274 138 133
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3345 3377 3840 3872 6660 6692 3345
Size: 32 463 32 2788 32 3353
Fragments arranged by size:
3353 2788 463 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6700
XbaI T'CTAG_A
Cuts at: 4036 4036
Size: 6700
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 3296 3545 3812 4829 3296
Size: 249 267 1017 5167
Fragments arranged by size:
5167 1017 267 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI NruI PacI
PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI
SrfI SwaI XcmI