Restriction map for
pFN21ASDA0211
(Circular) (Six-base) MAPSORT of: pFN21ASDA0211.seq Check: 948 from: 1 to: 8530
pFN21ASDA0211 8530 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4675 5162 4675
Size: 487 8043
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5261 6340 278
Size: 53 83 186 936 3725 1079 2468
Fragments arranged by size:
3725 2468 1079 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5873 1937
Size: 3936 4594
Acc65I G'GTAC_C
Cuts at: 2810 5058 5851 2810
Size: 2248 793 5489
Fragments arranged by size:
5489 2248 793
AclI AA'CG_TT
Cuts at: 1563 6657 7030 1563
Size: 5094 373 3063
Fragments arranged by size:
5094 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3576 4794 4941 6593 7977 8245 1632
Size: 1944 1218 147 1652 1384 268 1917
Fragments arranged by size:
1944 1917 1652 1384 1218 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8333
AflIII A'CryG_T
Cuts at: 1203 4750 5016 7429 8406 1203
Size: 3547 266 2413 977 1327
Fragments arranged by size:
3547 2413 1327 977 266
AgeI A'CCGG_T
Cuts at: 7361 7361
Size: 8530
AhdI GACnn_n'nnGTC
Cuts at: 4792 7259 4792
Size: 2467 6063
AleI CACnn'nnGTG
Cuts at: 1166 2340 3930 4434 4506 4871 1166
Size: 1174 1590 504 72 365 4825
Fragments arranged by size:
4825 1590 1174 504 365 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3500 4910 1184
Size: 2316 1410 4804
Fragments arranged by size:
4804 2316 1410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 5070 5102 3989
Size: 32 1049 32 7417
Fragments arranged by size:
7417 1049 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2468 3215 3976 4130 4264 4287 6273 7845 2468
Size: 747 761 154 134 23 1986 1572 3153
Fragments arranged by size:
3153 1986 1572 761 747 154 134 23
ApaI G_GGCC'C
Cuts at: 2964 3423 5735 2964
Size: 459 2312 5759
Fragments arranged by size:
5759 2312 459
ApaLI G'TGCA_C
Cuts at: 3530 3871 6587 7743 3530
Size: 341 2716 1156 4317
Fragments arranged by size:
4317 2716 1156 341
ApoI r'AATT_y
Cuts at: 1493 2348 3334 5839 6065 6217 8326 1493
Size: 855 986 2505 226 152 2109 1697
Fragments arranged by size:
2505 2109 1697 986 855 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7084 160
Size: 872 6052 1606
Fragments arranged by size:
6052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3423 4842 5425 5432 5855 1870
Size: 73 856 624 1419 583 7 423 4545
Fragments arranged by size:
4545 1419 856 624 583 423 73 7
AvrII C'CTAG_G
Cuts at: 2831 2831
Size: 8530
BamHI G'GATC_C
Cuts at: 2473 2713 3201 3759 5860 6227 2473
Size: 240 488 558 2101 367 4776
Fragments arranged by size:
4776 2101 558 488 367 240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2810 3298 3634 4033 5058
Size: 325 217 627 1023 488 336 399 1025
Cuts at: 5058 5342 5647 5851 7306 618
Size: 284 305 204 1455 1842
Fragments arranged by size:
1842 1455 1025 1023 627 488 399 336
325 305 284 217 204
BanII G_rGCy'C
Cuts at: 1391 2315 2964 3026 3347 3423 3954 5433 5636
Size: 924 649 62 321 76 531 1479 203
Cuts at: 5636 5735 5849 1391
Size: 99 114 4072
Fragments arranged by size:
4072 1479 924 649 531 321 203 114
99 76 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3582 3676 4621 4687 4800 928
Size: 971 1683 94 945 66 113 4658
Fragments arranged by size:
4658 1683 971 945 113 94 66
BbvCI CC'TCA_GC
Cuts at: 4955 5454 5525 4955
Size: 499 71 7960
Fragments arranged by size:
7960 499 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 5707 5741 6721 6755 1903
Size: 34 1997 34 1739 34 980 34 3678
Fragments arranged by size:
3678 1997 1739 980 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6422 7632 6422
Size: 1210 7320
BclI T'GATC_A
Cuts at: 1548 2614 5764 1548
Size: 1066 3150 4314
Fragments arranged by size:
4314 3150 1066
BfrBI ATG'CAT
Cuts at: 5227 5227
Size: 8530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 2888 2897
Size: 107 122 71 631 605 433 783 9
Cuts at: 2897 4836 7141 136
Size: 1939 2305 1525
Fragments arranged by size:
2305 1939 1525 783 631 605 433 122
107 71 9
BglII A'GATC_T
Cuts at: 2317 8525 2317
Size: 6208 2322
BlpI GC'TnA_GC
Cuts at: 5947 5947
Size: 8530
Bme1580I G_kGCm'C
Cuts at: 1790 2964 3423 3534 3639 3720 3875 4294 4706
Size: 1174 459 111 105 81 155 419 412
Cuts at: 4706 4715 5652 5735 6591 7747 1790
Size: 9 937 83 856 1156 2573
Fragments arranged by size:
2573 1174 1156 937 856 459 419 412
155 111 105 83 81 9
BmgBI CAC'GTC
Cuts at: 1318 4753 1318
Size: 3435 5095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2115 2280 2558
Size: 416 53 83 399 24 698 165 278
Cuts at: 2558 6261 7219 442
Size: 3703 958 1753
Fragments arranged by size:
3703 1753 958 698 416 399 278 165
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5650 5650
Size: 8530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2310 2423 2440 2591 3050 3812 4089
Size: 897 174 113 17 151 459 762 277
Cuts at: 4089 4787 5225 5322 7190 1239
Size: 698 438 97 1868 2579
Fragments arranged by size:
2579 1868 897 762 698 459 438 277
174 151 113 97 17
Bpu10I CC'TnA_GC
Cuts at: 3827 4955 5454 5525 3827
Size: 1128 499 71 6832
Fragments arranged by size:
6832 1128 499 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 6655 7520 7818 4810
Size: 1845 865 298 5522
Fragments arranged by size:
5522 1845 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5553 7193 882
Size: 617 4054 1640 2219
Fragments arranged by size:
4054 2219 1640 617
BsaAI yAC'GTr
Cuts at: 493 1204 3766 5017 5827 6321 493
Size: 711 2562 1251 810 494 2702
Fragments arranged by size:
2702 2562 1251 810 711 494
BsaBI GATnn'nnATC
Cuts at: 8524 8524
Size: 8530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4692 5258 6337 6719
Size: 53 83 186 936 3159 566 1079 382
Cuts at: 6719 7354 275
Size: 635 1451
Fragments arranged by size:
3159 1451 1079 936 635 566 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2590 2716 4143 6963 7361 7635 7782 8193 2590
Size: 126 1427 2820 398 274 147 411 2927
Fragments arranged by size:
2927 2820 1427 411 398 274 147 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 4577 4607 1178
Size: 30 165 30 1147 30 1997 30 5101
Fragments arranged by size:
5101 1997 1147 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 4092 4241 5480 5582 5585 5588 5597
Size: 955 1951 149 1239 102 3 3 9
Cuts at: 5597 5642 5657 5734 1186
Size: 45 15 77 3982
Fragments arranged by size:
3982 1951 1239 955 149 102 77 45
15 9 3 3
BseYI C'CCAG_C
Cuts at: 3275 3404 3969 4045 4566 4578 4596 4908 5180
Size: 129 565 76 521 12 18 312 272
Cuts at: 5180 5728 7733 8229 3275
Size: 548 2005 496 3576
Fragments arranged by size:
3576 2005 565 548 521 496 312 272
129 76 18 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3931 4188 4300 4379 4748 5149 1163
Size: 573 2195 257 112 79 369 401 4544
Fragments arranged by size:
4544 2195 573 401 369 257 112 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 5044 5980 6741 7769 1540
Size: 275 186 216 2827 936 761 1028 2301
Fragments arranged by size:
2827 2301 1028 936 761 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 3315 3479 3534 3875 3884 4616 4763 6591 6676
Size: 164 55 341 9 732 147 1828 85
Cuts at: 6676 7747 3315
Size: 1071 4098
Fragments arranged by size:
4098 1828 1071 732 341 164 147 85
55 9
BsmI GAATG_Cn'
Cuts at: 4511 6041 6134 4511
Size: 1530 93 6907
Fragments arranged by size:
6907 1530 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 8530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2315 2964 3026 3315 3347 3423 3479
Size: 399 525 649 62 289 32 76 56
Cuts at: 3479 3534 3639 3720 3875 3884 3954 4294 4616
Size: 55 105 81 155 9 70 340 322
Cuts at: 4616 4706 4715 4763 5433 5636 5652 5735 5849
Size: 90 9 48 670 203 16 83 114
Cuts at: 5849 6591 6676 7747 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 649 525 399 340
322 289 203 155 114 105 90 85
83 81 76 70 62 56 55 48
32 16 9 9
BspEI T'CCGG_A
Cuts at: 2716 8193 2716
Size: 5477 3053
BspHI T'CATG_A
Cuts at: 6419 8149 6419
Size: 1730 6800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 4253 4675 4982 5162 5885 844
Size: 1978 1431 422 307 180 723 3489
Fragments arranged by size:
3489 1978 1431 723 422 307 180
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 6417 1394
Size: 429 2533 2061 3507
Fragments arranged by size:
3507 2533 2061 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3261 5356 7025 7199 65
Size: 1183 2013 2095 1669 174 1396
Fragments arranged by size:
2095 2013 1669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 3769 3843 5067 5687 7174 7361
Size: 110 402 1557 74 1224 620 1487 187
Cuts at: 7361 1700
Size: 2869
Fragments arranged by size:
2869 1557 1487 1224 620 402 187 110
74
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8530
BssSI C'ACGA_G
Cuts at: 2675 6590 7602 2675
Size: 3915 1012 3603
Fragments arranged by size:
3915 3603 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3879 794
Size: 3085 5445
BstBI TT'CG_AA
Cuts at: 2007 7334 2007
Size: 5327 3203
BstEII G'GTnAC_C
Cuts at: 1184 3243 1184
Size: 2059 6471
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 2908 3839 4873 2342
Size: 566 931 1034 5999
Fragments arranged by size:
5999 1034 931 566
BstYI r'GATC_y
Cuts at: 1972 2317 2329 2473 2713 2950 3201 3211 3759
Size: 345 12 144 240 237 251 10 548
Cuts at: 3759 5860 6227 6612 6629 8081 8167 8179 8525
Size: 2101 367 385 17 1452 86 12 346
Cuts at: 8525 1972
Size: 1977
Fragments arranged by size:
2101 1977 1452 548 385 367 346 345
251 240 237 144 86 17 12 12
10
Bsu36I CC'TnA_GG
Cuts at: 2469 3321 5292 5742 5817 2469
Size: 852 1971 450 75 5182
Fragments arranged by size:
5182 1971 852 450 75
BtgI C'CryG_G
Cuts at: 513 1065 2022 4029 4521 4765 4949 5038 5652
Size: 552 957 2007 492 244 184 89 614
Cuts at: 5652 513
Size: 3391
Fragments arranged by size:
3391 2007 957 614 552 492 244 184
89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7791
Fragments arranged by size:
7791 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3482 3968 4093 4486 4545 5078 5613 6046
Size: 394 486 125 393 59 533 535 433
Cuts at: 6046 6840 6860 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 535 533 486 433 394 393
125 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2074 2109 529
Size: 35 1510 35 6950
Fragments arranged by size:
6950 1510 35 35
DraI TTT'AAA
Cuts at: 5834 5834
Size: 8530
DraIII CAC_nnn'GTG
Cuts at: 2123 3652 3879 5464 5554 2123
Size: 1529 227 1585 90 5099
Fragments arranged by size:
5099 1585 1529 227 90
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3586 4193 7537 809
Size: 507 2270 607 3344 1802
Fragments arranged by size:
3344 2270 1802 607 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2938 3537
Size: 54 1425 50 275 18 384 724 599
Cuts at: 3537 3771 3841 4903 5755 5977 6866 8495 8
Size: 234 70 1062 852 222 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 852 724 599 384
275 234 222 70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2214 5977 1537
Size: 275 402 3763 4090
Fragments arranged by size:
4090 3763 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3303 3815 3902 4634 5371 5543 5574 6460
Size: 1950 512 87 732 737 172 31 886
Cuts at: 6460 1353
Size: 3423
Fragments arranged by size:
3423 1950 886 737 732 512 172 87
31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3862 7117 7491 7637 387
Size: 1064 138 2273 3255 374 146 1280
Fragments arranged by size:
3255 2273 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2310 2423 2440 2591 3050 3576
Size: 393 504 174 113 17 151 459 526
Cuts at: 3576 3812 4089 4787 4794 4941 5225 5322 6593
Size: 236 277 698 7 147 284 97 1271
Cuts at: 6593 7190 7977 8245 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1271 787 698 597 526 504 459
393 284 277 268 236 174 151 147
113 97 17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3324 3324
Size: 8530
EcoO109I rG'GnC_Cy
Cuts at: 2960 3271 3420 3427 3694 4345 2960
Size: 311 149 7 267 651 7145
Fragments arranged by size:
7145 651 311 267 149 7
EcoRI G'AATT_C
Cuts at: 5839 6217 5839
Size: 378 8152
FspI TGC'GCA
Cuts at: 5108 7036 5108
Size: 1928 6602
HaeII r_GCGC'y
Cuts at: 1678 3261 7677 1678
Size: 1583 4416 2531
Fragments arranged by size:
4416 2531 1583
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 3383 3415 4778
Size: 32 135 32 978 32 801 32 1363
Cuts at: 4778 4808 4810 4840 5026 5058 5684 5716 7171
Size: 30 2 30 186 32 626 32 1455
Cuts at: 7171 7203 7245 7277 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1455 1363 978 801 626 186 135
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5774 5874 6120 669
Size: 988 281 3836 100 246 3079
Fragments arranged by size:
3836 3079 988 281 246 100
HindIII A'AGCT_T
Cuts at: 748 2742 748
Size: 1994 6536
HpaI GTT'AAC
Cuts at: 6120 6120
Size: 8530
KpnI G_GTAC'C
Cuts at: 2814 5062 5855 2814
Size: 2248 793 5489
Fragments arranged by size:
5489 2248 793
MfeI C'AATT_G
Cuts at: 6129 6129
Size: 8530
MluI A'CGCG_T
Cuts at: 8406 8406
Size: 8530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3732 5024 7644 7828 1305
Size: 2427 1292 2620 184 2007
Fragments arranged by size:
2620 2427 2007 1292 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2940 3539 5757 10
Size: 54 1425 343 1108 599 2218 2783
Fragments arranged by size:
2783 2218 1425 1108 599 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2340 3930 4434 4506 4871 6488
Size: 648 996 178 1590 504 72 365 1617
Cuts at: 6488 6847 7006 518
Size: 359 159 2042
Fragments arranged by size:
2042 1617 1590 996 648 504 365 359
178 159 72
MspA1I CmG'CkG
Cuts at: 1606 3408 3973 4300 4814 4908 4951 5597 5705
Size: 1802 565 327 514 94 43 646 108
Cuts at: 5705 5946 6623 7771 8016 1606
Size: 241 677 1148 245 2120
Fragments arranged by size:
2120 1802 1148 677 646 565 514 327
245 241 108 94 43
NaeI GCC'GGC
Cuts at: 2214 3771 3845 5069 2214
Size: 1557 74 1224 5675
Fragments arranged by size:
5675 1557 1224 74
NcoI C'CATG_G
Cuts at: 513 1065 2022 4521 5652 513
Size: 552 957 2499 1131 3391
Fragments arranged by size:
3391 2499 1131 957 552
NdeI CA'TA_TG
Cuts at: 387 2159 2607 387
Size: 1772 448 6310
Fragments arranged by size:
6310 1772 448
NgoMIV G'CCGG_C
Cuts at: 2212 3769 3843 5067 2212
Size: 1557 74 1224 5675
Fragments arranged by size:
5675 1557 1224 74
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8530
NotI GC'GGCC_GC
Cuts at: 5977 5977
Size: 8530
NsiI A_TGCA'T
Cuts at: 5229 5229
Size: 8530
NspI r_CATG'y
Cuts at: 1132 1229 5888 7433 1132
Size: 97 4659 1545 2229
Fragments arranged by size:
4659 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3349 1348
Size: 2001 6529
PciI A'CATG_T
Cuts at: 7429 7429
Size: 8530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3976 5321 1108
Size: 2868 1345 4317
Fragments arranged by size:
4317 2868 1345
PfoI T'CCnGG_A
Cuts at: 2568 4476 2568
Size: 1908 6622
PmeI GTTT'AAAC
Cuts at: 5834 5834
Size: 8530
PmlI CAC'GTG
Cuts at: 3766 3766
Size: 8530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6599 6631 8112 8144 6599
Size: 32 1481 32 6985
Fragments arranged by size:
6985 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3427 3694 4345 3427
Size: 267 651 7612
Fragments arranged by size:
7612 651 267
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8530
PsiI TTA'TAA
Cuts at: 4608 6100 4608
Size: 1492 7038
PspOMI G'GGCC_C
Cuts at: 2960 3419 5731 2960
Size: 459 2312 5759
Fragments arranged by size:
5759 2312 459
PspXI vC'TCGA_Gb
Cuts at: 5432 5432
Size: 8530
PstI C_TGCA'G
Cuts at: 830 3083 3413 3489 4552 5882 830
Size: 2253 330 76 1063 1330 3478
Fragments arranged by size:
3478 2253 1330 1063 330 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8530
PvuII CAG'CTG
Cuts at: 3408 3973 5705 3408
Size: 565 1732 6233
Fragments arranged by size:
6233 1732 565
SacII CC_GC'GG
Cuts at: 4952 4952
Size: 8530
SalI G'TCGA_C
Cuts at: 1936 5872 1936
Size: 3936 4594
SanDI GG'GwC_CC
Cuts at: 3427 3427
Size: 8530
SapI GCTCTTCn'nnn_
Cuts at: 3303 3815 3902 3303
Size: 512 87 7931
Fragments arranged by size:
7931 512 87
SbfI CC_TGCA'GG
Cuts at: 5882 5882
Size: 8530
ScaI AGT'ACT
Cuts at: 6778 8490 6778
Size: 1712 6818
SexAI A'CCwGG_T
Cuts at: 3648 5460 3648
Size: 1812 6718
SfcI C'TryA_G
Cuts at: 826 1045 3079 3409 3485 4417 4548 5878 7013
Size: 219 2034 330 76 932 131 1330 1135
Cuts at: 7013 7694 7885 826
Size: 681 191 1471
Fragments arranged by size:
2034 1471 1330 1135 932 681 330 219
191 131 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2897 2897
Size: 8530
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8530
SmaI CCC'GGG
Cuts at: 1872 2801 3425 5857 1872
Size: 929 624 2432 4545
Fragments arranged by size:
4545 2432 929 624
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4825 4842 5432 6634 7535 7797
Size: 197 926 2882 17 590 1202 901 262
Cuts at: 7797 820
Size: 1553
Fragments arranged by size:
2882 1553 1202 926 901 590 262 197
17
SnaBI TAC'GTA
Cuts at: 493 5827 6321 493
Size: 5334 494 2702
Fragments arranged by size:
5334 2702 494
SpeI A'CTAG_T
Cuts at: 152 4868 152
Size: 4716 3814
SphI G_CATG'C
Cuts at: 1132 5888 1132
Size: 4756 3774
SspI AAT'ATT
Cuts at: 5 52 6454 5
Size: 47 6402 2081
Fragments arranged by size:
6402 2081 47
StuI AGG'CCT
Cuts at: 1517 4918 5783 1517
Size: 3401 865 4264
Fragments arranged by size:
4264 3401 865
StyI C'CwwG_G
Cuts at: 513 1065 2022 2100 2655 2787 2831 2883 4452
Size: 552 957 78 555 132 44 52 1569
Cuts at: 4452 4521 5652 5969 513
Size: 69 1131 317 3074
Fragments arranged by size:
3074 1569 1131 957 555 552 317 132
78 69 52 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2134 2532 4580 4592 4778 6557 6574
Size: 1255 554 398 2048 12 186 1779 17
Cuts at: 6574 6727 6912 7357 325
Size: 153 185 445 1498
Fragments arranged by size:
2048 1779 1498 1255 554 445 398 186
185 153 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6776 8488 96
Size: 274 80 33 51 1443 4799 1712 138
Fragments arranged by size:
4799 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4577 4609 5795 5827 8490 8522 4577
Size: 32 1186 32 2663 32 4585
Fragments arranged by size:
4585 2663 1186 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8530
XbaI T'CTAG_A
Cuts at: 5866 5866
Size: 8530
XhoI C'TCGA_G
Cuts at: 1943 4842 5432 1943
Size: 2899 590 5041
Fragments arranged by size:
5041 2899 590
XmaI C'CCGG_G
Cuts at: 1870 2799 3423 5855 1870
Size: 929 624 2432 4545
Fragments arranged by size:
4545 2432 929 624
XmnI GAAnn'nnTTC
Cuts at: 6659 6659
Size: 8530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5259 6338 276
Size: 53 83 186 936 3725 1079 2468
Fragments arranged by size:
3725 2468 1079 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI PacI PsrI
RsrII SacI SfoI SrfI SwaI XcmI