Restriction map for
pFN21ASDA0200
(Circular) (Six-base) MAPSORT of: pFN21ASDA0200.seq Check: 9795 from: 1 to: 7777
pFN21ASDA0200 7777 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2045 2138 3146 2045
Size: 93 1008 6676
Fragments arranged by size:
6676 1008 93
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5587 278
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5120 1937
Size: 3183 4594
Acc65I G'GTAC_C
Cuts at: 5098 5098
Size: 7777
AclI AA'CG_TT
Cuts at: 1563 5904 6277 1563
Size: 4341 373 3063
Fragments arranged by size:
4341 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3947 4185 4828 5010 5033 5840 7224
Size: 1058 1257 238 643 182 23 807 1384
Cuts at: 7224 7492 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1257 1058 807 643 268 238
182 23
AfeI AGC'GCT
Cuts at: 2207 2207
Size: 7777
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7580
AflIII A'CryG_T
Cuts at: 1203 6676 7653 1203
Size: 5473 977 1327
Fragments arranged by size:
5473 1327 977
AgeI A'CCGG_T
Cuts at: 4492 6608 4492
Size: 2116 5661
AhdI GACnn_n'nnGTC
Cuts at: 2866 6506 2866
Size: 3640 4137
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2759 3246 1184
Size: 1575 487 5715
Fragments arranged by size:
5715 1575 487
AlwNI CAG_nnn'CTG
Cuts at: 2130 3661 4712 4829 4930 5520 7092 2130
Size: 1531 1051 117 101 590 1572 2815
Fragments arranged by size:
2815 1572 1531 1051 590 117 101
ApaI G_GGCC'C
Cuts at: 3182 3327 4190 3182
Size: 145 863 6769
Fragments arranged by size:
6769 863 145
ApaLI G'TGCA_C
Cuts at: 4743 5834 6990 4743
Size: 1091 1156 5530
Fragments arranged by size:
5530 1156 1091
ApoI r'AATT_y
Cuts at: 1493 2924 4528 5086 5312 5464 7573 1493
Size: 1431 1604 558 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 1431 558 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2910 6331 160
Size: 872 1878 3421 1606
Fragments arranged by size:
3421 1878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2315 3084 3292 3610 4149 5102
Size: 73 216 156 769 208 318 539 953
Cuts at: 5102 1870
Size: 4545
Fragments arranged by size:
4545 953 769 539 318 216 208 156
73
AvrII C'CTAG_G
Cuts at: 3043 3043
Size: 7777
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4022 4055 4022
Size: 33 7744
BamHI G'GATC_C
Cuts at: 5107 5474 5107
Size: 367 7410
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 2272 2806 3283 5098
Size: 325 217 627 328 157 534 477 1815
Cuts at: 5098 6553 618
Size: 1455 1842
Fragments arranged by size:
1842 1815 1455 627 534 477 328 325
217 157
BanII G_rGCy'C
Cuts at: 1391 2784 3053 3151 3182 3327 4190 4706 4820
Size: 1393 269 98 31 145 863 516 114
Cuts at: 4820 4856 4971 5096 1391
Size: 36 115 125 4072
Fragments arranged by size:
4072 1393 863 516 269 145 125 115
114 98 36 31
BbeI G_GCGC'C
Cuts at: 2119 2276 2119
Size: 157 7620
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2444 2694 2823 2862 928
Size: 971 545 250 129 39 5843
Fragments arranged by size:
5843 971 545 250 129 39
BbvCI CC'TCA_GC
Cuts at: 4794 4794
Size: 7777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5968 6002 1903
Size: 34 4031 34 3678
Fragments arranged by size:
4031 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5669 6879 5669
Size: 1210 6567
BclI T'GATC_A
Cuts at: 1548 2703 1548
Size: 1155 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2285 3048
Size: 107 122 71 631 605 592 21 763
Cuts at: 3048 3281 3641 4293 4684 6388 136
Size: 233 360 652 391 1704 1525
Fragments arranged by size:
1704 1525 763 652 631 605 592 391
360 233 122 107 71 21
BglII A'GATC_T
Cuts at: 7772 7772
Size: 7777
BlpI GC'TnA_GC
Cuts at: 4705 5194 4705
Size: 489 7288
Bme1580I G_kGCm'C
Cuts at: 1790 2811 3182 3327 4110 4190 4680 4747 5838
Size: 1021 371 145 783 80 490 67 1091
Cuts at: 5838 6994 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1091 1021 783 490 371 145
80 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 2718 3093
Size: 416 53 83 399 24 1211 90 375
Cuts at: 3093 3165 4030 4730 5508 6466 442
Size: 72 865 700 778 958 1753
Fragments arranged by size:
1753 1211 958 865 778 700 416 399
375 90 83 72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3462 3703 3220
Size: 242 241 7294
Fragments arranged by size:
7294 242 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2195 2475 2547 2587 2916 3203 3242
Size: 950 6 280 72 40 329 287 39
Cuts at: 3242 3452 4123 4597 6437 1239
Size: 210 671 474 1840 2579
Fragments arranged by size:
2579 1840 950 671 474 329 287 280
210 72 40 39 6
Bpu10I CC'TnA_GC
Cuts at: 4794 4814 4830 4794
Size: 20 16 7741
Fragments arranged by size:
7741 20 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5902 6767 7065 5902
Size: 865 298 6614
Fragments arranged by size:
6614 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5070 6440 882
Size: 617 3571 1370 2219
Fragments arranged by size:
3571 2219 1370 617
BsaAI yAC'GTr
Cuts at: 493 1204 5074 5568 493
Size: 711 3870 494 2702
Fragments arranged by size:
3870 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7771 7771
Size: 7777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2116 2273 2301 2535
Size: 53 83 186 936 583 157 28 234
Cuts at: 2535 5584 5966 6601 275
Size: 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 583 382 234 186
157 83 53 28
BsaWI w'CCGG_w
Cuts at: 4492 6210 6608 6882 7029 7440 4492
Size: 1718 398 274 147 411 4829
Fragments arranged by size:
4829 1718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3830 3860 1178
Size: 30 165 30 2427 30 5095
Fragments arranged by size:
5095 2427 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 2864 5045 1186
Size: 1618 60 2181 3918
Fragments arranged by size:
3918 2181 1618 60
BseYI C'CCAG_C
Cuts at: 3038 3473 3654 3807 4166 4326 4446 4771 4819
Size: 435 181 153 359 160 120 325 48
Cuts at: 4819 4834 6980 7476 3038
Size: 15 2146 496 3339
Fragments arranged by size:
3339 2146 496 435 359 325 181 160
153 120 48 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 4871 4876 1163
Size: 573 447 2688 5 4064
Fragments arranged by size:
4064 2688 573 447 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2326 4876 5227 5988 7016 1540
Size: 275 186 325 2550 351 761 1028 2301
Fragments arranged by size:
2550 2301 1028 761 351 325 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 2784 4706 4747 5838 5923 6994 2322
Size: 462 1922 41 1091 85 1071 3105
Fragments arranged by size:
3105 1922 1091 1071 462 85 41
BsmI GAATG_Cn'
Cuts at: 4557 5288 5381 4557
Size: 731 93 6953
Fragments arranged by size:
6953 731 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4405 4405
Size: 7777
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2784 2811 3053 3151 3182 3327
Size: 399 532 462 27 242 98 31 145
Cuts at: 3327 4110 4190 4680 4706 4747 4820 4856 4971
Size: 783 80 490 26 41 73 36 115
Cuts at: 4971 5096 5838 5923 6994 1391
Size: 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 532 490 462 399
242 145 125 115 98 85 80 73
41 36 31 27 26
BspEI T'CCGG_A
Cuts at: 7440 7440
Size: 7777
BspHI T'CATG_A
Cuts at: 2344 3417 5666 7396 2344
Size: 1073 2249 1730 2725
Fragments arranged by size:
2725 2249 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 2138 2700 3146 3495 4063 5132 844
Size: 1201 93 562 446 349 568 1069 3489
Fragments arranged by size:
3489 1201 1069 568 562 446 349 93
BsrBI CCG'CTC
Cuts at: 1394 1823 5664 1394
Size: 429 3841 3507
Fragments arranged by size:
3841 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6272 6446 65
Size: 1183 5024 174 1396
Fragments arranged by size:
5024 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2112 3115 4492 6421 6608 1700
Size: 110 284 18 1003 1377 1929 187 2869
Fragments arranged by size:
2869 1929 1377 1003 284 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7777
BssHII G'CGCG_C
Cuts at: 1925 2230 1925
Size: 305 7472
BssSI C'ACGA_G
Cuts at: 3677 5837 6849 3677
Size: 2160 1012 4605
Fragments arranged by size:
4605 2160 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3898 4804 794
Size: 3104 906 3767
Fragments arranged by size:
3767 3104 906
BstBI TT'CG_AA
Cuts at: 2007 6581 2007
Size: 4574 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7777
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 7777
BstYI r'GATC_y
Cuts at: 1972 5107 5474 5859 5876 7328 7414 7426 7772
Size: 3135 367 385 17 1452 86 12 346
Cuts at: 7772 1972
Size: 1977
Fragments arranged by size:
3135 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 4161 4161
Size: 7777
BtgI C'CryG_G
Cuts at: 513 1065 2022 2043 2063 2262 3357 3825 4367
Size: 552 957 21 20 199 1095 468 542
Cuts at: 4367 4515 4689 4716 4917 513
Size: 148 174 27 201 3373
Fragments arranged by size:
3373 1095 957 552 542 468 201 199
174 148 27 21 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7038
Fragments arranged by size:
7038 683 56
BtsI GCAGTG_nn'
Cuts at: 4602 4680 4888 4969 5293 6087 6107 4602
Size: 78 208 81 324 794 20 6272
Fragments arranged by size:
6272 794 324 208 81 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7777
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7742
DraI TTT'AAA
Cuts at: 5081 5081
Size: 7777
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6784 809
Size: 507 5468 1802
Fragments arranged by size:
5468 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2323 2331 3619
Size: 54 1425 50 275 18 493 8 1288
Cuts at: 3619 3748 3828 4567 5224 6113 7742 8
Size: 129 80 739 657 889 1629 43
Fragments arranged by size:
1629 1425 1288 889 739 657 493 275
129 80 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2323 5224 1537
Size: 275 511 2901 4090
Fragments arranged by size:
4090 2901 511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2479 5707 1353
Size: 1126 3228 3423
Fragments arranged by size:
3423 3228 1126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 3104 6364 6738 6884 387
Size: 1064 138 474 1041 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 1041 474 374 146 138
EcoICRI GAG'CTC
Cuts at: 2782 4704 2782
Size: 1922 5855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2195 2475 2547 2587 2690 2916
Size: 393 557 6 280 72 40 103 226
Cuts at: 2916 3203 3242 3452 3947 4123 4185 4597 4828
Size: 287 39 210 495 176 62 412 231
Cuts at: 4828 5010 5033 5840 6437 7224 7492 1239
Size: 182 23 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 557 495 412 393
287 280 268 231 226 210 182 176
103 72 62 40 39 23 6
EcoO109I rG'GnC_Cy
Cuts at: 2416 2452 2827 2860 3005 3178 3189 3323 3324
Size: 36 375 33 145 173 11 134 1
Cuts at: 3324 3458 3804 4092 4187 4464 4722 4826 4839
Size: 134 346 288 95 277 258 104 13
Cuts at: 4839 2416
Size: 5354
Fragments arranged by size:
5354 375 346 288 277 258 173 145
134 134 104 95 36 33 13 11
1
EcoRI G'AATT_C
Cuts at: 2924 4528 5086 5464 2924
Size: 1604 558 378 5237
Fragments arranged by size:
5237 1604 558 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 7777
FspI TGC'GCA
Cuts at: 6283 6283
Size: 7777
HaeII r_GCGC'y
Cuts at: 1678 2089 2119 2167 2182 2209 2276 2376 4015
Size: 411 30 48 15 27 67 100 1639
Cuts at: 4015 4764 6924 1678
Size: 749 2160 2531
Fragments arranged by size:
2531 2160 1639 749 411 100 67 48
30 27 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2454 2486 2852
Size: 32 135 32 566 32 284 32 366
Cuts at: 2852 2884 3631 3663 4858 4890 6418 6450 6492
Size: 32 747 32 1195 32 1528 32 42
Cuts at: 6492 6524 1373
Size: 32 2626
Fragments arranged by size:
2626 1528 1195 747 566 366 284 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5121 5367 669
Size: 988 281 3183 246 3079
Fragments arranged by size:
3183 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7777
HpaI GTT'AAC
Cuts at: 5367 5367
Size: 7777
KasI G'GCGC_C
Cuts at: 2115 2272 2115
Size: 157 7620
KpnI G_GTAC'C
Cuts at: 5102 5102
Size: 7777
MfeI C'AATT_G
Cuts at: 5376 5376
Size: 7777
MluI A'CGCG_T
Cuts at: 7653 7653
Size: 7777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4057 4158 6891 7075 1305
Size: 2752 101 2733 184 2007
Fragments arranged by size:
2752 2733 2007 184 101
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3621 3750 3830 4569 10
Size: 54 1425 343 1789 129 80 739 3218
Fragments arranged by size:
3218 1789 1425 739 343 129 80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2616 5735 6094 6253 518
Size: 648 1177 273 3119 359 159 2042
Fragments arranged by size:
3119 2042 1177 648 359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2306 3661 3878 4143 4406 4712 4919
Size: 459 241 1355 217 265 263 306 207
Cuts at: 4919 5193 5870 7018 7263 1606
Size: 274 677 1148 245 2120
Fragments arranged by size:
2120 1355 1148 677 459 306 274 265
263 245 241 217 207
NaeI GCC'GGC
Cuts at: 2096 2114 3117 2096
Size: 18 1003 6756
Fragments arranged by size:
6756 1003 18
NarI GG'CG_CC
Cuts at: 2116 2273 2116
Size: 157 7620
NcoI C'CATG_G
Cuts at: 513 1065 2022 2043 3357 4515 4689 513
Size: 552 957 21 1314 1158 174 3601
Fragments arranged by size:
3601 1314 1158 957 552 174 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7777
NgoMIV G'CCGG_C
Cuts at: 2094 2112 3115 2094
Size: 18 1003 6756
Fragments arranged by size:
6756 1003 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7777
NotI GC'GGCC_GC
Cuts at: 5224 5224
Size: 7777
NspI r_CATG'y
Cuts at: 1132 1229 4199 4276 4358 4627 5135 6680 1132
Size: 97 2970 77 82 269 508 1545 2229
Fragments arranged by size:
2970 2229 1545 508 269 97 82 77
PasI CC'CwG_GG
Cuts at: 1348 4577 1348
Size: 3229 4548
PciI A'CATG_T
Cuts at: 6676 6676
Size: 7777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7777
PmeI GTTT'AAAC
Cuts at: 5081 5081
Size: 7777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5846 5878 7359 7391 5846
Size: 32 1481 32 6232
Fragments arranged by size:
6232 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 2827 2860 3804 4092 4464 4722 2416
Size: 411 33 944 288 372 258 5471
Fragments arranged by size:
5471 944 411 372 288 258 33
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7777
PsiI TTA'TAA
Cuts at: 5347 5347
Size: 7777
PspOMI G'GGCC_C
Cuts at: 3178 3323 4186 3178
Size: 145 863 6769
Fragments arranged by size:
6769 863 145
PspXI vC'TCGA_Gb
Cuts at: 4149 4149
Size: 7777
PstI C_TGCA'G
Cuts at: 830 2544 2989 4609 5129 830
Size: 1714 445 1620 520 3478
Fragments arranged by size:
3478 1714 1620 520 445
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7777
PvuII CAG'CTG
Cuts at: 3661 4143 4406 4712 3661
Size: 482 263 306 6726
Fragments arranged by size:
6726 482 306 263
RsrII CG'GwC_CG
Cuts at: 4914 4914
Size: 7777
SacI G_AGCT'C
Cuts at: 2784 4706 2784
Size: 1922 5855
SacII CC_GC'GG
Cuts at: 2066 4920 2066
Size: 2854 4923
SalI G'TCGA_C
Cuts at: 1936 5119 1936
Size: 3183 4594
SanDI GG'GwC_CC
Cuts at: 3804 4464 3804
Size: 660 7117
SbfI CC_TGCA'GG
Cuts at: 5129 5129
Size: 7777
ScaI AGT'ACT
Cuts at: 6025 7737 6025
Size: 1712 6065
SfcI C'TryA_G
Cuts at: 826 1045 2540 2985 4605 4942 5125 6260 6941
Size: 219 1495 445 1620 337 183 1135 681
Cuts at: 6941 7132 826
Size: 191 1471
Fragments arranged by size:
1620 1495 1471 1135 681 445 337 219
191 183
SfoI GGC'GCC
Cuts at: 2117 2274 2117
Size: 157 7620
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
SgrAI Cr'CCGG_yG
Cuts at: 1700 2094 1700
Size: 394 7383
SmaI CCC'GGG
Cuts at: 1872 3086 3294 3612 5104 1872
Size: 1214 208 318 1492 4545
Fragments arranged by size:
4545 1492 1214 318 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4149 5881 6782 7044 820
Size: 197 926 2206 1732 901 262 1553
Fragments arranged by size:
2206 1732 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5074 5568 493
Size: 4581 494 2702
Fragments arranged by size:
4581 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7777
SphI G_CATG'C
Cuts at: 1132 5135 1132
Size: 4003 3774
SspI AAT'ATT
Cuts at: 5 52 2914 5701 5
Size: 47 2862 2787 2081
Fragments arranged by size:
2862 2787 2081 47
StuI AGG'CCT
Cuts at: 1517 4901 1517
Size: 3384 4393
StyI C'CwwG_G
Cuts at: 513 1065 2022 2043 2220 2895 3043 3357 4122
Size: 552 957 21 177 675 148 314 765
Cuts at: 4122 4499 4515 4689 5216 513
Size: 377 16 174 527 3074
Fragments arranged by size:
3074 957 765 675 552 527 377 314
177 174 148 21 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 4910 5804 5821 5974 6159 6604
Size: 1255 915 2415 894 17 153 185 445
Cuts at: 6604 325
Size: 1498
Fragments arranged by size:
2415 1498 1255 915 894 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3026 6023 7735
Size: 274 80 33 51 1443 1049 2997 1712
Cuts at: 7735 96
Size: 138
Fragments arranged by size:
2997 1712 1443 1049 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 4111 4143 7737 7769 3830
Size: 32 249 32 3594 32 3838
Fragments arranged by size:
3838 3594 249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7777
XbaI T'CTAG_A
Cuts at: 5113 5113
Size: 7777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 4687 2041
Size: 2646 5131
XhoI C'TCGA_G
Cuts at: 1943 4149 1943
Size: 2206 5571
XmaI C'CCGG_G
Cuts at: 1870 3084 3292 3610 5102 1870
Size: 1214 208 318 1492 4545
Fragments arranged by size:
4545 1492 1214 318 208
XmnI GAAnn'nnTTC
Cuts at: 5906 5906
Size: 7777
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5585 276
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PsrI
SapI SexAI SfiI SrfI SwaI