Restriction map for
pFN21ASDA0199
(Circular) (Six-base) MAPSORT of: pFN21ASDA0199.seq Check: 6173 from: 1 to: 8566
pFN21ASDA0199 8566 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4652 4652
Size: 8566
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3800 4140 6376 278
Size: 53 83 186 936 2264 340 2236 2468
Fragments arranged by size:
2468 2264 2236 936 340 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 3543 3618 5730 5909 1937
Size: 1204 402 75 2112 179 4594
Fragments arranged by size:
4594 2112 1204 402 179 75
Acc65I G'GTAC_C
Cuts at: 5887 5887
Size: 8566
AclI AA'CG_TT
Cuts at: 1563 6693 7066 1563
Size: 5130 373 3063
Fragments arranged by size:
5130 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 3299 3916 4741 5351 6629 8013 8281
Size: 990 677 617 825 610 1278 1384 268
Cuts at: 8281 1632
Size: 1917
Fragments arranged by size:
1917 1384 1278 990 825 677 617 610
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8369
AflIII A'CryG_T
Cuts at: 1203 7465 8442 1203
Size: 6262 977 1327
Fragments arranged by size:
6262 1327 977
AgeI A'CCGG_T
Cuts at: 7397 7397
Size: 8566
AhdI GACnn_n'nnGTC
Cuts at: 7295 7295
Size: 8566
AleI CACnn'nnGTG
Cuts at: 1166 2201 3251 5393 1166
Size: 1035 1050 2142 4339
Fragments arranged by size:
4339 2142 1050 1035
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2769 1184
Size: 1585 6981
AlwNI CAG_nnn'CTG
Cuts at: 2183 3322 4056 5574 5620 5773 6309 7881 2183
Size: 1139 734 1518 46 153 536 1572 2868
Fragments arranged by size:
2868 1572 1518 1139 734 536 153 46
ApaI G_GGCC'C
Cuts at: 4074 4112 4257 4604 4912 4074
Size: 38 145 347 308 7728
Fragments arranged by size:
7728 347 308 145 38
ApaLI G'TGCA_C
Cuts at: 2843 5250 6623 7779 2843
Size: 2407 1373 1156 3630
Fragments arranged by size:
3630 2407 1373 1156
ApoI r'AATT_y
Cuts at: 1493 2189 5875 6101 6253 8362 1493
Size: 696 3686 226 152 2109 1697
Fragments arranged by size:
3686 2109 1697 696 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7120 160
Size: 872 6088 1606
Fragments arranged by size:
6088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2382 4009 4256 4310 4596 4700
Size: 73 115 324 1627 247 54 286 104
Cuts at: 4700 4734 4880 5891 1870
Size: 34 146 1011 4545
Fragments arranged by size:
4545 1627 1011 324 286 247 146 115
104 73 54 34
BamHI G'GATC_C
Cuts at: 5896 6263 5896
Size: 367 8199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 3590 4032 4710 5887
Size: 325 217 627 495 1308 442 678 1177
Cuts at: 5887 7342 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1308 1177 678 627 495 442
325 217
BanII G_rGCy'C
Cuts at: 1391 2082 2877 3677 3694 4074 4112 4257 4604
Size: 691 795 800 17 380 38 145 347
Cuts at: 4604 4707 4912 5820 5885 1391
Size: 103 205 908 65 4072
Fragments arranged by size:
4072 908 800 795 691 380 347 205
145 103 65 38 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2731 3492 928
Size: 971 832 761 6002
Fragments arranged by size:
6002 971 832 761
BbvCI CC'TCA_GC
Cuts at: 3412 5141 3412
Size: 1729 6837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3013 3047 3631 3665 4942 4976 6757
Size: 34 1076 34 584 34 1277 34 1781
Cuts at: 6757 6791 1903
Size: 34 3678
Fragments arranged by size:
3678 1781 1277 1076 584 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6458 7668 6458
Size: 1210 7356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2291 4261 4407
Size: 107 122 71 631 605 619 1970 146
Cuts at: 4407 4599 7177 136
Size: 192 2578 1525
Fragments arranged by size:
2578 1970 1525 631 619 605 192 146
122 107 71
BglII A'GATC_T
Cuts at: 8561 8561
Size: 8566
BlpI GC'TnA_GC
Cuts at: 2871 2993 5819 5983 2871
Size: 122 2826 164 5454
Fragments arranged by size:
5454 2826 164 122
Bme1580I G_kGCm'C
Cuts at: 1790 2287 2782 2847 3258 4074 4089 4112 4257
Size: 497 495 65 411 816 15 23 145
Cuts at: 4257 4604 4912 5247 5254 5838 6627 7783 1790
Size: 347 308 335 7 584 789 1156 2573
Fragments arranged by size:
2573 1156 816 789 584 497 495 411
347 335 308 145 65 23 15 7
BmgBI CAC'GTC
Cuts at: 1318 4414 1318
Size: 3096 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 3848 3983
Size: 416 53 83 399 24 1158 1273 135
Cuts at: 3983 4099 4908 5313 6297 7255 442
Size: 116 809 405 984 958 1753
Fragments arranged by size:
1753 1273 1158 984 958 809 416 405
399 135 116 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3371 1056
Size: 2315 6251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4035 4891 4906 5056 5059 4035
Size: 856 15 150 3 7542
Fragments arranged by size:
7542 856 150 15 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3167 4043 4954 5009 5178 5360 5867 7226
Size: 1928 876 911 55 169 182 507 1359
Cuts at: 7226 1239
Size: 2579
Fragments arranged by size:
2579 1928 1359 911 876 507 182 169
55
Bpu10I CC'TnA_GC
Cuts at: 2501 2517 3176 3412 3777 3816 4512 5141 2501
Size: 16 659 236 365 39 696 629 5926
Fragments arranged by size:
5926 696 659 629 365 236 39 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4526 6691 7556 7854 4526
Size: 2165 865 298 5238
Fragments arranged by size:
5238 2165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2722 3133 5044 5120 7229 882
Size: 617 1223 411 1911 76 2109 2219
Fragments arranged by size:
2219 2109 1911 1223 617 411 76
BsaAI yAC'GTr
Cuts at: 493 1204 2419 2842 5863 6357 493
Size: 711 1215 423 3021 494 2702
Fragments arranged by size:
3021 2702 1215 711 494 423
BsaBI GATnn'nnATC
Cuts at: 3608 8560 3608
Size: 4952 3614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3041 3797 4137 5001
Size: 53 83 186 936 1508 756 340 864
Cuts at: 5001 6373 6755 7390 275
Size: 1372 382 635 1451
Fragments arranged by size:
1508 1451 1372 936 864 756 635 382
340 186 83 53
BsaWI w'CCGG_w
Cuts at: 2409 6999 7397 7671 7818 8229 2409
Size: 4590 398 274 147 411 2746
Fragments arranged by size:
4590 2746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 5333 5363 1178
Size: 30 165 30 1041 30 2859 30 4381
Fragments arranged by size:
4381 2859 1041 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2417 2468 2870 4574 4802 5032 5048
Size: 886 345 51 402 1704 228 230 16
Cuts at: 5048 1186
Size: 4704
Fragments arranged by size:
4704 1704 886 402 345 230 228 51
16
BseYI C'CCAG_C
Cuts at: 2801 3220 3628 3731 4082 4162 4243 4521 5288
Size: 419 408 103 351 80 81 278 767
Cuts at: 5288 5754 7769 8265 2801
Size: 466 2015 496 3102
Fragments arranged by size:
3102 2015 767 496 466 419 408 351
278 103 81 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 3007 4142 4790 4792 5073 1163
Size: 573 1044 227 1135 648 2 281 4656
Fragments arranged by size:
4656 1135 1044 648 573 281 227 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4803 4984 5394 6016 6777
Size: 275 186 2047 755 181 410 622 761
Cuts at: 6777 7805 1540
Size: 1028 2301
Fragments arranged by size:
2301 2047 1028 761 755 622 410 275
186 181
BsiHKAI G_wGCw'C
Cuts at: 2847 2877 2994 3114 5254 5820 6627 6712 7783
Size: 30 117 120 2140 566 807 85 1071
Cuts at: 7783 2847
Size: 3630
Fragments arranged by size:
3630 2140 1071 807 566 120 117 85
30
BsmI GAATG_Cn'
Cuts at: 2387 4455 6077 6170 2387
Size: 2068 1622 93 4783
Fragments arranged by size:
4783 2068 1622 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4128 4307 4128
Size: 179 8387
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2287 2782 2847 2877 2994 3114
Size: 399 292 205 495 65 30 117 120
Cuts at: 3114 3258 3677 3694 4074 4089 4112 4257 4604
Size: 144 419 17 380 15 23 145 347
Cuts at: 4604 4707 4912 5247 5254 5820 5838 5885 6627
Size: 103 205 335 7 566 18 47 742
Cuts at: 6627 6712 7783 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 495 419 399 380
347 335 292 205 205 145 144 120
117 103 85 65 47 30 23 18
17 15 7
BspEI T'CCGG_A
Cuts at: 2409 8229 2409
Size: 5820 2746
BspHI T'CATG_A
Cuts at: 3198 6455 8185 3198
Size: 3257 1730 3579
Fragments arranged by size:
3579 3257 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 3522 4087 4652 5667 5921 844
Size: 1643 1035 565 565 1015 254 3489
Fragments arranged by size:
3489 1643 1035 1015 565 565 254
BsrBI CCG'CTC
Cuts at: 1394 1823 3448 4731 5057 6453 1394
Size: 429 1625 1283 326 1396 3507
Fragments arranged by size:
3507 1625 1396 1283 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 7061 7235 65
Size: 1183 5813 174 1396
Fragments arranged by size:
5813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4029 4175 4840 4983 7210 7397 1700
Size: 110 2219 146 665 143 2227 187 2869
Fragments arranged by size:
2869 2227 2219 665 187 146 143 110
BsrGI T'GTAC_A
Cuts at: 96 4146 5199 5397 96
Size: 4050 1053 198 3265
Fragments arranged by size:
4050 3265 1053 198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8566
BssSI C'ACGA_G
Cuts at: 2615 5347 6626 7638 2615
Size: 2732 1279 1012 3543
Fragments arranged by size:
3543 2732 1279 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8566
BstBI TT'CG_AA
Cuts at: 2007 7370 2007
Size: 5363 3203
BstEII G'GTnAC_C
Cuts at: 1184 3865 1184
Size: 2681 5885
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2764 3124 3220 3424 5419 2734
Size: 30 360 96 204 1995 5881
Fragments arranged by size:
5881 1995 360 204 96 30
BstYI r'GATC_y
Cuts at: 1972 2406 5769 5896 6263 6648 6665 8117 8203
Size: 434 3363 127 367 385 17 1452 86
Cuts at: 8203 8215 8561 1972
Size: 12 346 1977
Fragments arranged by size:
3363 1977 1452 434 385 367 346 127
86 17 12
BstZ17I GTA'TAC
Cuts at: 3619 3619
Size: 8566
Bsu36I CC'TnA_GG
Cuts at: 2492 3396 3879 4038 4860 5044 2492
Size: 904 483 159 822 184 6014
Fragments arranged by size:
6014 904 822 483 184 159
BtgI C'CryG_G
Cuts at: 513 1065 2074 2979 3213 4129 5412 5500 513
Size: 552 1009 905 234 916 1283 88 3579
Fragments arranged by size:
3579 1283 1009 916 905 552 234 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7827
Fragments arranged by size:
7827 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 4057 5133 5554 5818 6082 6876 6896 2150
Size: 1907 1076 421 264 264 794 20 3820
Fragments arranged by size:
3820 1907 1076 794 421 264 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8531
DraI TTT'AAA
Cuts at: 5870 5870
Size: 8566
DraIII CAC_nnn'GTG
Cuts at: 5242 5242
Size: 8566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3854 7573 809
Size: 507 2538 3719 1802
Fragments arranged by size:
3719 2538 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3210 3920 3954
Size: 54 1425 50 275 18 1380 710 34
Cuts at: 3954 4045 4170 4342 4368 4726 4800 4981 5421
Size: 91 125 172 26 358 74 181 440
Cuts at: 5421 5674 6013 6902 8531 8
Size: 253 339 889 1629 43
Fragments arranged by size:
1629 1425 1380 889 710 440 358 339
275 253 181 172 125 91 74 54
50 43 34 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 4800 4981 6013 1537
Size: 275 2233 755 181 1032 4090
Fragments arranged by size:
4090 2233 1032 755 275 181
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2702 3035 3935 4625 5363 6496 1353
Size: 767 582 333 900 690 738 1133 3423
Fragments arranged by size:
3423 1133 900 767 738 690 582 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2840 3902 3989 4279 4596 4987
Size: 1064 138 1251 1062 87 290 317 391
Cuts at: 4987 7153 7527 7673 387
Size: 2166 374 146 1280
Fragments arranged by size:
2166 1280 1251 1064 1062 391 374 317
290 146 138 87
EcoICRI GAG'CTC
Cuts at: 2875 5818 2875
Size: 2943 5623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 3167 3299 3916 4043 4741 4954
Size: 393 990 545 132 617 127 698 213
Cuts at: 4954 5009 5178 5351 5360 5867 6629 7226 8013
Size: 55 169 173 9 507 762 597 787
Cuts at: 8013 8281 1239
Size: 268 1524
Fragments arranged by size:
1524 990 787 762 698 617 597 545
507 393 268 213 173 169 132 127
55 9
EcoO109I rG'GnC_Cy
Cuts at: 2180 3875 4070 4100 4108 4600 4601 4855 4909
Size: 1695 195 30 8 492 1 254 54
Cuts at: 4909 5163 5617 5698 5713 2180
Size: 254 454 81 15 5033
Fragments arranged by size:
5033 1695 492 454 254 254 195 81
54 30 15 8 1
EcoRI G'AATT_C
Cuts at: 2189 5875 6253 2189
Size: 3686 378 4502
Fragments arranged by size:
4502 3686 378
EcoRV GAT'ATC
Cuts at: 2055 2055
Size: 8566
FspI TGC'GCA
Cuts at: 3641 7072 3641
Size: 3431 5135
HaeII r_GCGC'y
Cuts at: 1678 3577 4163 4480 5296 7713 1678
Size: 1899 586 317 816 2417 2531
Fragments arranged by size:
2531 2417 1899 816 586 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 3176 3208 5102
Size: 32 135 32 467 32 1105 32 1894
Cuts at: 5102 5134 5498 5530 7207 7239 7281 7313 1373
Size: 32 364 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1894 1677 1105 467 364 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5910 6156 669
Size: 988 281 3972 246 3079
Fragments arranged by size:
3972 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5635 748
Size: 4887 3679
HpaI GTT'AAC
Cuts at: 6156 6156
Size: 8566
KpnI G_GTAC'C
Cuts at: 5891 5891
Size: 8566
MfeI C'AATT_G
Cuts at: 6165 6165
Size: 8566
MluI A'CGCG_T
Cuts at: 8442 8442
Size: 8566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 7680 7864 1305
Size: 1111 5264 184 2007
Fragments arranged by size:
5264 2007 1111 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3212 3956 4172 4370 5423
Size: 54 1425 343 1380 744 216 198 1053
Cuts at: 5423 10
Size: 3153
Fragments arranged by size:
3153 1425 1380 1053 744 343 216 198
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2751 3011 3251 3707 4964 5393
Size: 648 1035 550 260 240 456 1257 429
Cuts at: 5393 6524 6883 7042 518
Size: 1131 359 159 2042
Fragments arranged by size:
2042 1257 1131 1035 648 550 456 429
359 260 240 159
MspA1I CmG'CkG
Cuts at: 1606 2685 3454 4243 4500 4761 4774 4980 5285
Size: 1079 769 789 257 261 13 206 305
Cuts at: 5285 5982 6659 7807 8052 1606
Size: 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 1079 789 769 697 677 305
261 257 245 206 13
NaeI GCC'GGC
Cuts at: 4031 4985 4031
Size: 954 7612
NcoI C'CATG_G
Cuts at: 513 1065 2074 4129 5412 513
Size: 552 1009 2055 1283 3667
Fragments arranged by size:
3667 2055 1283 1009 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8566
NgoMIV G'CCGG_C
Cuts at: 4029 4983 4029
Size: 954 7612
NheI G'CTAG_C
Cuts at: 1052 3367 1052
Size: 2315 6251
NotI GC'GGCC_GC
Cuts at: 6013 6013
Size: 8566
NspI r_CATG'y
Cuts at: 1132 1229 3724 4384 5924 7469 1132
Size: 97 2495 660 1540 1545 2229
Fragments arranged by size:
2495 2229 1545 1540 660 97
PasI CC'CwG_GG
Cuts at: 1348 2543 5168 1348
Size: 1195 2625 4746
Fragments arranged by size:
4746 2625 1195
PciI A'CATG_T
Cuts at: 7465 7465
Size: 8566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 4351 5413 5773 1108
Size: 1290 1953 1062 360 3901
Fragments arranged by size:
3901 1953 1290 1062 360
PfoI T'CCnGG_A
Cuts at: 3264 3264
Size: 8566
PmeI GTTT'AAAC
Cuts at: 5870 5870
Size: 8566
PmlI CAC'GTG
Cuts at: 2419 2842 2419
Size: 423 8143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6635 6667 8148 8180 6635
Size: 32 1481 32 7021
Fragments arranged by size:
7021 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2180 4100 5163 5617 5698 5713 2180
Size: 1920 1063 454 81 15 5033
Fragments arranged by size:
5033 1920 1063 454 81 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8566
PsiI TTA'TAA
Cuts at: 6136 6136
Size: 8566
PspOMI G'GGCC_C
Cuts at: 4070 4108 4253 4600 4908 4070
Size: 38 145 347 308 7728
Fragments arranged by size:
7728 347 308 145 38
PstI C_TGCA'G
Cuts at: 830 2634 2816 4950 5027 5154 5918 830
Size: 1804 182 2134 77 127 764 3478
Fragments arranged by size:
3478 2134 1804 764 182 127 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8566
PvuII CAG'CTG
Cuts at: 3454 4243 4761 3454
Size: 789 518 7259
Fragments arranged by size:
7259 789 518
SacI G_AGCT'C
Cuts at: 2877 5820 2877
Size: 2943 5623
SalI G'TCGA_C
Cuts at: 1936 5908 1936
Size: 3972 4594
SanDI GG'GwC_CC
Cuts at: 5163 5163
Size: 8566
SapI GCTCTTCn'nnn_
Cuts at: 3935 3935
Size: 8566
SbfI CC_TGCA'GG
Cuts at: 5918 5918
Size: 8566
ScaI AGT'ACT
Cuts at: 6814 8526 6814
Size: 1712 6854
SfcI C'TryA_G
Cuts at: 826 1045 2630 2812 4946 5023 5150 5914 7049
Size: 219 1585 182 2134 77 127 764 1135
Cuts at: 7049 7730 7921 826
Size: 681 191 1471
Fragments arranged by size:
2134 1585 1471 1135 764 681 219 191
182 127 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8566
SmaI CCC'GGG
Cuts at: 1872 2384 4258 4598 5893 1872
Size: 512 1874 340 1295 4545
Fragments arranged by size:
4545 1874 1295 512 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4505 6670 7571 7833 820
Size: 197 926 2562 2165 901 262 1553
Fragments arranged by size:
2562 2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5863 6357 493
Size: 5370 494 2702
Fragments arranged by size:
5370 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8566
SphI G_CATG'C
Cuts at: 1132 3724 4384 5924 1132
Size: 2592 660 1540 3774
Fragments arranged by size:
3774 2592 1540 660
SrfI GCCC'GGGC
Cuts at: 4258 4258
Size: 8566
SspI AAT'ATT
Cuts at: 5 52 6490 5
Size: 47 6438 2081
Fragments arranged by size:
6438 2081 47
StuI AGG'CCT
Cuts at: 1517 2490 3174 3401 1517
Size: 973 684 227 6682
Fragments arranged by size:
6682 973 684 227
StyI C'CwwG_G
Cuts at: 513 1065 2074 3169 4104 4129 5121 5412 6005
Size: 552 1009 1095 935 25 992 291 593
Cuts at: 6005 513
Size: 3074
Fragments arranged by size:
3074 1095 1009 992 935 593 552 291
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2489 3671 4235 4668 5630 6593
Size: 1255 690 219 1182 564 433 962 963
Cuts at: 6593 6610 6763 6948 7393 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1182 963 962 690 564 445
433 219 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 4146 5199
Size: 274 80 33 51 1443 702 1467 1053
Cuts at: 5199 5397 5461 6812 8524 96
Size: 198 64 1351 1712 138
Fragments arranged by size:
1712 1467 1443 1351 1053 702 274 198
138 80 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2444 2476 4346 4378 5333 5365 5731 5763 8526
Size: 32 1870 32 955 32 366 32 2763
Cuts at: 8526 8558 2444
Size: 32 2452
Fragments arranged by size:
2763 2452 1870 955 366 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 1316
Size: 1629 6937
XbaI T'CTAG_A
Cuts at: 5902 5902
Size: 8566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2710 2962 3867 4936 2710
Size: 252 905 1069 6340
Fragments arranged by size:
6340 1069 905 252
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8566
XmaI C'CCGG_G
Cuts at: 1870 2382 4256 4596 5891 1870
Size: 512 1874 340 1295 4545
Fragments arranged by size:
4545 1874 1295 512 340
XmnI GAAnn'nnTTC
Cuts at: 6695 6695
Size: 8566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3798 4138 6374 276
Size: 53 83 186 936 2264 340 2236 2468
Fragments arranged by size:
2468 2264 2236 936 340 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SwaI