Restriction map for
pFN21ASDA0194
(Circular) (Six-base) MAPSORT of: pFN21ASDA0194.seq Check: 8806 from: 1 to: 8605
pFN21ASDA0194 8605 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4825 5021 5070 4825
Size: 196 49 8360
Fragments arranged by size:
8360 196 49
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6415 278
Size: 53 83 186 936 4879 2468
Fragments arranged by size:
4879 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3918 5948 1937
Size: 1981 2030 4594
Fragments arranged by size:
4594 2030 1981
Acc65I G'GTAC_C
Cuts at: 5926 5926
Size: 8605
AclI AA'CG_TT
Cuts at: 1563 6732 7105 1563
Size: 5169 373 3063
Fragments arranged by size:
5169 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 3725 4565 4760 6668 8052 8320 1632
Size: 2078 15 840 195 1908 1384 268 1917
Fragments arranged by size:
2078 1917 1908 1384 840 268 195 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8408
AflIII A'CryG_T
Cuts at: 1203 7504 8481 1203
Size: 6301 977 1327
Fragments arranged by size:
6301 1327 977
AgeI A'CCGG_T
Cuts at: 7436 7436
Size: 8605
AhdI GACnn_n'nnGTC
Cuts at: 3684 7334 3684
Size: 3650 4955
AleI CACnn'nnGTG
Cuts at: 1166 3016 5336 1166
Size: 1850 2320 4435
Fragments arranged by size:
4435 2320 1850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2581 3641 4127 1184
Size: 1397 1060 486 5662
Fragments arranged by size:
5662 1397 1060 486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 2903 2935 2474
Size: 32 397 32 8144
Fragments arranged by size:
8144 397 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2569 2621 3223 4099 4367 4609 4970 5062 5489
Size: 52 602 876 268 242 361 92 427
Cuts at: 5489 6348 7920 2569
Size: 859 1572 3254
Fragments arranged by size:
3254 1572 876 859 602 427 361 268
242 92 52
ApaI G_GGCC'C
Cuts at: 4323 4323
Size: 8605
ApaLI G'TGCA_C
Cuts at: 6662 7818 6662
Size: 1156 7449
ApoI r'AATT_y
Cuts at: 1493 3160 3199 3942 4766 4868 5914 6140 6292
Size: 1667 39 743 824 102 1046 226 152
Cuts at: 6292 8401 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1667 1046 824 743 226 152
102 39
AseI AT'TA_AT
Cuts at: 160 1032 7159 160
Size: 872 6127 1606
Fragments arranged by size:
6127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 5930 1870
Size: 73 1371 2616 4545
Fragments arranged by size:
4545 2616 1371 73
AvrII C'CTAG_G
Cuts at: 3040 3040
Size: 8605
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2034 2067 3318 3351 2034
Size: 33 1251 33 7288
Fragments arranged by size:
7288 1251 33 33
BamHI G'GATC_C
Cuts at: 2329 3054 5935 6302 2329
Size: 725 2881 367 4632
Fragments arranged by size:
4632 2881 725 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5009 5583 5614 5926 7381
Size: 325 217 627 3222 574 31 312 1455
Cuts at: 7381 618
Size: 1842
Fragments arranged by size:
3222 1842 1455 627 574 325 312 217
31
BanII G_rGCy'C
Cuts at: 1391 2214 2605 3514 4016 4050 4323 4778 5454
Size: 823 391 909 502 34 273 455 676
Cuts at: 5454 5924 1391
Size: 470 4072
Fragments arranged by size:
4072 909 823 676 502 470 455 391
273 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4141 4577 5103 928
Size: 971 2242 436 526 4430
Fragments arranged by size:
4430 2242 971 526 436
BbvCI CC'TCA_GC
Cuts at: 2457 2589 2651 4143 2457
Size: 132 62 1492 6919
Fragments arranged by size:
6919 1492 132 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3920 3954 6796 6830 1903
Size: 34 1983 34 2842 34 3678
Fragments arranged by size:
3678 2842 1983 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6497 7707 6497
Size: 1210 7395
BclI T'GATC_A
Cuts at: 1548 3276 1548
Size: 1728 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2550 3641 5891
Size: 107 122 71 631 605 878 1091 2250
Cuts at: 5891 7216 136
Size: 1325 1525
Fragments arranged by size:
2250 1525 1325 1091 878 631 605 122
107 71
BglII A'GATC_T
Cuts at: 8600 8600
Size: 8605
BlpI GC'TnA_GC
Cuts at: 4592 6022 4592
Size: 1430 7175
Bme1580I G_kGCm'C
Cuts at: 1790 2541 2787 3023 4323 5012 5216 5266 5586
Size: 751 246 236 1300 689 204 50 320
Cuts at: 5586 5606 6666 7822 1790
Size: 20 1060 1156 2573
Fragments arranged by size:
2573 1300 1156 1060 751 689 320 246
236 204 50 20
BmgBI CAC'GTC
Cuts at: 1318 5180 5334 1318
Size: 3862 154 4589
Fragments arranged by size:
4589 3862 154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2605 3587 5504
Size: 416 53 83 399 24 1188 982 1917
Cuts at: 5504 5568 6336 7294 442
Size: 64 768 958 1753
Fragments arranged by size:
1917 1753 1188 982 958 768 416 399
83 64 53 24
BmtI G_CTAG'C
Cuts at: 1056 4736 1056
Size: 3680 4925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2866 4030 4246 5050 2472
Size: 394 1164 216 804 6027
Fragments arranged by size:
6027 1164 804 394 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3215 3429 3433 3498 4985 7265 1239
Size: 1976 214 4 65 1487 2280 2579
Fragments arranged by size:
2579 2280 1976 1487 214 65 4
Bpu10I CC'TnA_GC
Cuts at: 2457 2589 2651 4052 4143 5066 2457
Size: 132 62 1401 91 923 5996
Fragments arranged by size:
5996 1401 923 132 91 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6730 7595 7893 6730
Size: 865 298 7442
Fragments arranged by size:
7442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 4442 7268 882
Size: 617 1230 1713 2826 2219
Fragments arranged by size:
2826 2219 1713 1230 617
BsaAI yAC'GTr
Cuts at: 493 1204 5902 6396 493
Size: 711 4698 494 2702
Fragments arranged by size:
4698 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8599 8599
Size: 8605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3102 3344 6412 6794
Size: 53 83 186 936 1569 242 3068 382
Cuts at: 6794 7429 275
Size: 635 1451
Fragments arranged by size:
3068 1569 1451 936 635 382 242 186
83 53
BsaWI w'CCGG_w
Cuts at: 2368 2445 3978 7038 7436 7710 7857 8268 2368
Size: 77 1533 3060 398 274 147 411 2705
Fragments arranged by size:
3060 2705 1533 411 398 274 147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2745 2775 2903 2933 3248
Size: 30 165 30 1342 30 128 30 315
Cuts at: 3248 3278 3402 3432 4598 4628 4908 4938 1178
Size: 30 124 30 1166 30 280 30 4845
Fragments arranged by size:
4845 1342 1166 315 280 165 128 124
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2299 2496 2606 2842 3225 3269 4220 4601
Size: 1113 197 110 236 383 44 951 381
Cuts at: 4601 4729 4736 4929 5873 5876 5891 1186
Size: 128 7 193 944 3 15 3900
Fragments arranged by size:
3900 1113 951 944 383 381 236 197
193 128 110 44 15 7 3
BseYI C'CCAG_C
Cuts at: 2540 3712 3950 4316 5579 7808 8304 2540
Size: 1172 238 366 1263 2229 496 2841
Fragments arranged by size:
2841 2229 1263 1172 496 366 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2334 2841 3386 3826 4394 4574
Size: 573 486 112 507 545 440 568 180
Cuts at: 4574 4979 1163
Size: 405 4789
Fragments arranged by size:
4789 573 568 545 507 486 440 405
180 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6055 6816 7844 1540
Size: 275 186 4054 761 1028 2301
Fragments arranged by size:
4054 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2667 2984 3514 3535 4016 4058 5454 5496
Size: 453 317 530 21 481 42 1396 42
Cuts at: 5496 6666 6751 7822 2214
Size: 1170 85 1071 2997
Fragments arranged by size:
2997 1396 1170 1071 530 481 453 317
85 42 42 21
BsmI GAATG_Cn'
Cuts at: 4042 6116 6209 4042
Size: 2074 93 6438
Fragments arranged by size:
6438 2074 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3109 3109
Size: 8605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2541 2605 2667 2787 2984 3023
Size: 399 424 327 64 62 120 197 39
Cuts at: 3023 3514 3535 4016 4050 4058 4323 4778 5012
Size: 491 21 481 34 8 265 455 234
Cuts at: 5012 5216 5266 5454 5496 5586 5606 5924 6666
Size: 204 50 188 42 90 20 318 742
Cuts at: 6666 6751 7822 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 481 455 424 399
327 318 265 234 204 197 188 120
90 85 64 62 50 42 39 34
21 20 8
BspEI T'CCGG_A
Cuts at: 2368 8268 2368
Size: 5900 2705
BspHI T'CATG_A
Cuts at: 2826 4162 4908 6494 8224 2826
Size: 1336 746 1586 1730 3207
Fragments arranged by size:
3207 1730 1586 1336 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3498 4825 5021 5070 5289 5960 844
Size: 2654 1327 196 49 219 671 3489
Fragments arranged by size:
3489 2654 1327 671 219 196 49
BsrBI CCG'CTC
Cuts at: 1394 1823 6492 1394
Size: 429 4669 3507
Fragments arranged by size:
4669 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4624 5679 7100 7274 65
Size: 1183 3376 1055 1421 174 1396
Fragments arranged by size:
3376 1421 1396 1183 1055 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7249 7436 1700
Size: 110 5439 187 2869
Fragments arranged by size:
5439 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2623 5146 5776 96
Size: 2527 2523 630 2925
Fragments arranged by size:
2925 2527 2523 630
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8605
BssSI C'ACGA_G
Cuts at: 6665 7677 6665
Size: 1012 7593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2357 4099 4609 4847 5062 794
Size: 1563 1742 510 238 215 4337
Fragments arranged by size:
4337 1742 1563 510 238 215
BstBI TT'CG_AA
Cuts at: 2007 7409 2007
Size: 5402 3203
BstEII G'GTnAC_C
Cuts at: 1184 5567 1184
Size: 4383 4222
BstXI CCAn_nnnn'nTGG
Cuts at: 5338 5338
Size: 8605
BstYI r'GATC_y
Cuts at: 1972 2329 2391 2646 3054 4071 5763 5935 6302
Size: 357 62 255 408 1017 1692 172 367
Cuts at: 6302 6687 6704 8156 8242 8254 8600 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1692 1452 1017 408 385 367 357
346 255 172 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 3372 3372
Size: 8605
BtgI C'CryG_G
Cuts at: 513 1065 2022 2956 513
Size: 552 957 934 6162
Fragments arranged by size:
6162 957 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7866
Fragments arranged by size:
7866 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2570 4088 4271 4981 4987 5078 5895 6121
Size: 50 1518 183 710 6 91 817 226
Cuts at: 6121 6915 6935 2520
Size: 794 20 4190
Fragments arranged by size:
4190 1518 817 794 710 226 183 91
50 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8570
DraI TTT'AAA
Cuts at: 5909 5909
Size: 8605
DraIII CAC_nnn'GTG
Cuts at: 4266 4266
Size: 8605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7612 809
Size: 507 6296 1802
Fragments arranged by size:
6296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 5303 5328
Size: 54 1425 50 275 18 447 3026 25
Cuts at: 5328 5883 6052 6941 8570 8
Size: 555 169 889 1629 43
Fragments arranged by size:
3026 1629 1425 889 555 447 275 169
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 6052 1537
Size: 275 4240 4090
Fragments arranged by size:
4240 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 3112 4736 5040 5296 5868 6535 1353
Size: 1374 385 1624 304 256 572 667 3423
Fragments arranged by size:
3423 1624 1374 667 572 385 304 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5657 7192 7566 7712 387
Size: 1064 138 4068 1535 374 146 1280
Fragments arranged by size:
4068 1535 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 3512 4014 5452 2212
Size: 1300 502 1438 5365
Fragments arranged by size:
5365 1438 1300 502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3215 3429 3433 3498 3710 3725 4565
Size: 393 1583 214 4 65 212 15 840
Cuts at: 4565 4760 4985 6668 7265 8052 8320 1239
Size: 195 225 1683 597 787 268 1524
Fragments arranged by size:
1683 1583 1524 840 787 597 393 268
225 214 212 195 65 15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2588 2654 3673 4990 2438
Size: 150 66 1019 1317 6053
Fragments arranged by size:
6053 1317 1019 150 66
EcoO109I rG'GnC_Cy
Cuts at: 2501 2581 4320 4516 2501
Size: 80 1739 196 6590
Fragments arranged by size:
6590 1739 196 80
EcoRI G'AATT_C
Cuts at: 3199 4766 5914 6292 3199
Size: 1567 1148 378 5512
Fragments arranged by size:
5512 1567 1148 378
FspI TGC'GCA
Cuts at: 4103 7111 4103
Size: 3008 5597
HaeII r_GCGC'y
Cuts at: 1678 4081 7752 1678
Size: 2403 3671 2531
Fragments arranged by size:
3671 2531 2403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2795 2827 3670
Size: 32 135 32 749 32 442 32 843
Cuts at: 3670 3702 4115 4147 4241 4273 4515 4547 4908
Size: 32 413 32 94 32 242 32 361
Cuts at: 4908 4940 7246 7278 7320 7352 1373
Size: 32 2306 32 42 32 2626
Fragments arranged by size:
2626 2306 843 749 442 413 361 242
135 94 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3487 3598 3964 4930 5949 6195
Size: 988 281 1549 111 366 966 1019 246
Cuts at: 6195 669
Size: 3079
Fragments arranged by size:
3079 1549 1019 988 966 366 281 246
111
HindIII A'AGCT_T
Cuts at: 748 5018 748
Size: 4270 4335
HpaI GTT'AAC
Cuts at: 3598 3964 6195 3598
Size: 366 2231 6008
Fragments arranged by size:
6008 2231 366
KpnI G_GTAC'C
Cuts at: 5930 5930
Size: 8605
MfeI C'AATT_G
Cuts at: 6204 6204
Size: 8605
MluI A'CGCG_T
Cuts at: 8481 8481
Size: 8605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2240 2541 3798 4218 7719 7903 1305
Size: 935 301 1257 420 3501 184 2007
Fragments arranged by size:
3501 2007 1257 935 420 301 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5330 10
Size: 54 1425 343 3498 3285
Fragments arranged by size:
3498 3285 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3016 5336 6563 6922 7081 518
Size: 648 1850 2320 1227 359 159 2042
Fragments arranged by size:
2320 2042 1850 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3712 3840 3954 4096 4600 5674 6021 6698
Size: 2106 128 114 142 504 1074 347 677
Cuts at: 6698 7846 8091 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2106 1148 1074 677 504 347 245
142 128 114
NcoI C'CATG_G
Cuts at: 513 1065 2022 2956 513
Size: 552 957 934 6162
Fragments arranged by size:
6162 957 934 552
NdeI CA'TA_TG
Cuts at: 387 2035 2992 387
Size: 1648 957 6000
Fragments arranged by size:
6000 1648 957
NheI G'CTAG_C
Cuts at: 1052 4732 1052
Size: 3680 4925
NotI GC'GGCC_GC
Cuts at: 6052 6052
Size: 8605
NspI r_CATG'y
Cuts at: 1132 1229 2967 5963 7508 1132
Size: 97 1738 2996 1545 2229
Fragments arranged by size:
2996 2229 1738 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2975 3500 3630 1348
Size: 1627 525 130 6323
Fragments arranged by size:
6323 1627 525 130
PciI A'CATG_T
Cuts at: 7504 7504
Size: 8605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3298 4402 4970 5224 5593 1108
Size: 2190 1104 568 254 369 4120
Fragments arranged by size:
4120 2190 1104 568 369 254
PfoI T'CCnGG_A
Cuts at: 3406 5001 3406
Size: 1595 7010
PmeI GTTT'AAAC
Cuts at: 5909 5909
Size: 8605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 6674 6706 8187 8219 2903
Size: 32 3739 32 1481 32 3289
Fragments arranged by size:
3739 3289 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2501 4516 2501
Size: 2015 6590
PshAI GACnn'nnGTC
Cuts at: 1613 3347 1613
Size: 1734 6871
PsiI TTA'TAA
Cuts at: 6175 6175
Size: 8605
PspOMI G'GGCC_C
Cuts at: 4319 4319
Size: 8605
PstI C_TGCA'G
Cuts at: 830 2439 3562 4095 4116 4359 4605 5058 5957
Size: 1609 1123 533 21 243 246 453 899
Cuts at: 5957 830
Size: 3478
Fragments arranged by size:
3478 1609 1123 899 533 453 246 243
21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8605
PvuII CAG'CTG
Cuts at: 3712 3840 3954 4096 4600 3712
Size: 128 114 142 504 7717
Fragments arranged by size:
7717 504 142 128 114
SacI G_AGCT'C
Cuts at: 2214 3514 4016 5454 2214
Size: 1300 502 1438 5365
Fragments arranged by size:
5365 1438 1300 502
SalI G'TCGA_C
Cuts at: 1936 5947 1936
Size: 4011 4594
SapI GCTCTTCn'nnn_
Cuts at: 4736 4736
Size: 8605
SbfI CC_TGCA'GG
Cuts at: 2439 5957 2439
Size: 3518 5087
ScaI AGT'ACT
Cuts at: 6853 8565 6853
Size: 1712 6893
SfcI C'TryA_G
Cuts at: 826 1045 2435 3477 3558 4091 4112 4276 4355
Size: 219 1390 1042 81 533 21 164 79
Cuts at: 4355 4601 5038 5054 5552 5953 7088 7769 7960
Size: 246 437 16 498 401 1135 681 191
Cuts at: 7960 826
Size: 1471
Fragments arranged by size:
1471 1390 1135 1042 681 533 498 437
401 246 219 191 164 81 79 21
16
SfiI GGCCn_nnn'nGGCC
Cuts at: 3641 3641
Size: 8605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8605
SmaI CCC'GGG
Cuts at: 1872 3316 5932 1872
Size: 1444 2616 4545
Fragments arranged by size:
4545 2616 1444
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6709 7610 7872 820
Size: 197 926 4766 901 262 1553
Fragments arranged by size:
4766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5902 6396 493
Size: 5409 494 2702
Fragments arranged by size:
5409 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8605
SphI G_CATG'C
Cuts at: 1132 5963 1132
Size: 4831 3774
SspI AAT'ATT
Cuts at: 5 52 6529 5
Size: 47 6477 2081
Fragments arranged by size:
6477 2081 47
StuI AGG'CCT
Cuts at: 1517 3644 5550 1517
Size: 2127 1906 4572
Fragments arranged by size:
4572 2127 1906
StyI C'CwwG_G
Cuts at: 513 1065 2022 2739 2956 3040 3996 4986 5103
Size: 552 957 717 217 84 956 990 117
Cuts at: 5103 5592 5827 6044 513
Size: 489 235 217 3074
Fragments arranged by size:
3074 990 957 956 717 552 489 235
217 217 117 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 5523 6632 6649 6802 6987 7432
Size: 1255 542 3401 1109 17 153 185 445
Cuts at: 7432 325
Size: 1498
Fragments arranged by size:
3401 1498 1255 1109 542 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2623 3834
Size: 274 80 33 51 1443 198 448 1211
Cuts at: 3834 4030 5146 5776 6851 8563 96
Size: 196 1116 630 1075 1712 138
Fragments arranged by size:
1712 1443 1211 1116 1075 630 448 274
198 196 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 3248 3280 4598 4630 8565 8597 2745
Size: 32 471 32 1318 32 3935 32 2753
Fragments arranged by size:
3935 2753 1318 471 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8605
XbaI T'CTAG_A
Cuts at: 5941 5941
Size: 8605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2795 3082 3118 3190 4702 2773
Size: 22 287 36 72 1512 6676
Fragments arranged by size:
6676 1512 287 72 36 22
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8605
XmaI C'CCGG_G
Cuts at: 1870 3314 5930 1870
Size: 1444 2616 4545
Fragments arranged by size:
4545 2616 1444
XmnI GAAnn'nnTTC
Cuts at: 6734 6734
Size: 8605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6413 276
Size: 53 83 186 936 4879 2468
Fragments arranged by size:
4879 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BbeI BfrBI BsiWI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfoI SrfI SwaI