Restriction map for
pFN21ASDA0193
(Circular) (Six-base) MAPSORT of: pFN21ASDA0193.seq Check: 27 from: 1 to: 5971
pFN21ASDA0193 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4034 5418 5686 1632
Size: 2402 1384 268 1917
Fragments arranged by size:
2402 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2485 2789 1184
Size: 1301 304 4366
Fragments arranged by size:
4366 1301 304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2902 2934 3014 3046 3239 3271 2902
Size: 32 80 32 193 32 5602
Fragments arranged by size:
5602 193 80 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaLI G'TGCA_C
Cuts at: 2182 4028 5184 2182
Size: 1846 1156 2969
Fragments arranged by size:
2969 1846 1156
ApoI r'AATT_y
Cuts at: 1493 2101 3280 3506 3658 5767 1493
Size: 608 1179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1179 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 3296 1870
Size: 73 582 771 4545
Fragments arranged by size:
4545 771 582 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3290 1391
Size: 1899 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5000
BbvCI CC'TCA_GC
Cuts at: 3125 3125
Size: 5971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 2155 1548
Size: 607 5364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2186 3085 4032 5188 1790
Size: 396 899 947 1156 2573
Fragments arranged by size:
2573 1156 947 899 396
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 3702 4660
Size: 416 53 83 399 24 1137 1148 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1148 1137 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2661 2661
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 3161 3167 4631 1239
Size: 1449 473 6 1464 2579
Fragments arranged by size:
2579 1464 1449 473 6
Bpu10I CC'TnA_GC
Cuts at: 2073 3125 2073
Size: 1052 4919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 4634 882
Size: 617 1023 2112 2219
Fragments arranged by size:
2219 2112 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 2069 3086 3268 3762 493
Size: 711 865 1017 182 494 2702
Fragments arranged by size:
2702 1017 865 711 494 182
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2164 2626 4404 4802 5076 5223 5634 2164
Size: 462 1778 398 274 147 411 2501
Fragments arranged by size:
2501 1778 462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2847 2877 2971 3001 3014
Size: 30 165 30 1444 30 94 30 13
Cuts at: 3014 3044 3239 3269 1178
Size: 30 195 30 3880
Fragments arranged by size:
3880 1444 195 165 94 30 30 30
30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 3142 1186
Size: 839 1117 4015
Fragments arranged by size:
4015 1117 839
BseYI C'CCAG_C
Cuts at: 2344 5174 5670 2344
Size: 2830 496 2645
Fragments arranged by size:
2830 2645 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 1163
Size: 573 1059 4339
Fragments arranged by size:
4339 1059 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2526 3134 4032 4117 5188 2186
Size: 340 608 898 85 1071 2969
Fragments arranged by size:
2969 1071 898 608 340 85
BsmI GAATG_Cn'
Cuts at: 2892 3482 3575 2892
Size: 590 93 5288
Fragments arranged by size:
5288 590 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2526 3085 3134 3290 4032 4117
Size: 399 396 340 559 49 156 742 85
Cuts at: 4117 5188 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 396 340 156
85 49
BspEI T'CCGG_A
Cuts at: 2626 5634 2626
Size: 3008 2963
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 4615 4802 1700
Size: 110 1241 1564 187 2869
Fragments arranged by size:
2869 1564 1241 187 110
BsrGI T'GTAC_A
Cuts at: 96 3063 96
Size: 2967 3004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 3074 4031 5043 3074
Size: 957 1012 4002
Fragments arranged by size:
4002 1012 957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 2007 4775 2007
Size: 2768 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2611
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2353 3301 3668 4053 4070 5522 5608 5620
Size: 381 948 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 948 385 381 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3168 513
Size: 552 957 1146 3316
Fragments arranged by size:
3316 1146 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2642 2677 529
Size: 35 2078 35 3823
Fragments arranged by size:
3823 2078 35 35
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3901 1353
Size: 2548 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 3161 3167 4034 4631 5418 5686
Size: 393 1056 473 6 867 597 787 268
Cuts at: 5686 1239
Size: 1524
Fragments arranged by size:
1524 1056 867 787 597 473 393 268
6
EcoO109I rG'GnC_Cy
Cuts at: 2214 3220 2214
Size: 1006 4965
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 2488 2520 2847
Size: 32 135 32 878 32 6 32 327
Cuts at: 2847 2879 4612 4644 4686 4718 1373
Size: 32 1733 32 42 32 2626
Fragments arranged by size:
2626 1733 878 327 135 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3241 3315 3561 669
Size: 988 281 1303 74 246 3079
Fragments arranged by size:
3079 1303 988 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 5085 5269 1305
Size: 1098 2682 184 2007
Fragments arranged by size:
2682 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2771 3929 4288 4447 518
Size: 648 1443 162 1158 359 159 2042
Fragments arranged by size:
2042 1443 1158 648 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 2324 2811 3387 4064 5212 5457 1606
Size: 718 487 576 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 576 487 245
NaeI GCC'GGC
Cuts at: 3053 3053
Size: 5971
NcoI C'CATG_G
Cuts at: 513 1065 2022 3168 513
Size: 552 957 1146 3316
Fragments arranged by size:
3316 1146 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NgoMIV G'CCGG_C
Cuts at: 3051 3051
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PfoI T'CCnGG_A
Cuts at: 3033 3033
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PmlI CAC'GTG
Cuts at: 2069 3086 2069
Size: 1017 4954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2695 3014 3046 3239 3271 4040 4072 5553
Size: 32 319 32 193 32 769 32 1481
Cuts at: 5553 5585 2663
Size: 32 3049
Fragments arranged by size:
3049 1481 769 319 193 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2214 3220 2214
Size: 1006 4965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2032 3323 830
Size: 1202 1291 3478
Fragments arranged by size:
3478 1291 1202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2324 2324
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 2543 4219 5931 2543
Size: 1676 1712 2583
Fragments arranged by size:
2583 1712 1676
SfcI C'TryA_G
Cuts at: 826 1045 2028 3319 4454 5135 5326 826
Size: 219 983 1291 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 983 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 4075 4976 5238 820
Size: 197 926 582 1550 901 262 1553
Fragments arranged by size:
1553 1550 926 901 582 262 197
SnaBI TAC'GTA
Cuts at: 493 3268 3762 493
Size: 2775 494 2702
Fragments arranged by size:
2775 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 3159 1517
Size: 1642 4329
StyI C'CwwG_G
Cuts at: 513 1065 2022 2210 2653 3168 3410 513
Size: 552 957 188 443 515 242 3074
Fragments arranged by size:
3074 957 552 515 443 242 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 3998 4015 4168 4353 4798 325
Size: 1255 929 1489 17 153 185 445 1498
Fragments arranged by size:
1498 1489 1255 929 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 3063 4217
Size: 274 80 33 51 1443 564 522 1154
Cuts at: 4217 5929 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1154 564 522 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 2719 3307 2719
Size: 588 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3142 3142
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 2525 1943
Size: 582 5389
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI