Restriction map for
pFN21ASDA0180
(Circular) (Six-base) MAPSORT of: pFN21ASDA0180.seq Check: 1602 from: 1 to: 5893
pFN21ASDA0180 5893 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2940 3039 3236 1937
Size: 1003 99 197 4594
Fragments arranged by size:
4594 1003 197 99
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2515 2798 2903 3038 3956 5340 5608 1632
Size: 883 283 105 135 918 1384 268 1917
Fragments arranged by size:
1917 1384 918 883 283 268 135 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2753 1184
Size: 1569 4324
AlwNI CAG_nnn'CTG
Cuts at: 3636 5208 3636
Size: 1572 4321
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3202 3428 3580 5689 1493
Size: 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 3218 1870
Size: 73 143 1132 4545
Fragments arranged by size:
4545 1132 143 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2359 3214 4669 618
Size: 325 217 627 240 332 855 1455 1842
Fragments arranged by size:
1842 1455 855 627 332 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2105 2183 2307 2608 2979 3212 1391
Size: 714 78 124 301 371 233 4072
Fragments arranged by size:
4072 714 371 301 233 124 78
BbeI G_GCGC'C
Cuts at: 2031 2031
Size: 5893
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 2687 3132 928
Size: 971 542 246 445 3689
Fragments arranged by size:
3689 971 542 445 246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 4504 136
Size: 107 122 71 631 605 367 2465 1525
Fragments arranged by size:
2465 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 2051 3310 2051
Size: 1259 4634
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2444 3954 5110 1790
Size: 254 400 1510 1156 2573
Fragments arranged by size:
2573 1510 1156 400 254
BmgBI CAC'GTC
Cuts at: 1318 2439 1318
Size: 1121 4772
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2608 2926 3624
Size: 416 53 83 399 24 1191 318 698
Cuts at: 3624 4582 442
Size: 958 1753
Fragments arranged by size:
1753 1191 958 698 416 399 318 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2586 2586
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2321 3110 4553 1239
Size: 1055 27 789 1443 2579
Fragments arranged by size:
2579 1443 1055 789 27
Bpu10I CC'TnA_GC
Cuts at: 2973 3168 2973
Size: 195 5698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4018 4883 5181 4018
Size: 865 298 4730
Fragments arranged by size:
4730 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3190 3684 493
Size: 711 1986 494 2702
Fragments arranged by size:
2702 1986 711 494
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3700 4082 4717
Size: 53 83 186 936 495 1672 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1672 1451 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2584 4326 4724 4998 5145 5556 2584
Size: 1742 398 274 147 411 2921
Fragments arranged by size:
2921 1742 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3071 3101 1178
Size: 30 165 30 1668 30 3970
Fragments arranged by size:
3970 1668 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3079 1186
Size: 1893 4000
BseYI C'CCAG_C
Cuts at: 2415 2608 5096 5592 2415
Size: 193 2488 496 2716
Fragments arranged by size:
2716 2488 496 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 2118 3343 4104 5132 1540
Size: 275 186 47 70 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186 70 47
BsiHKAI G_wGCw'C
Cuts at: 2979 3954 4039 5110 2979
Size: 975 85 1071 3762
Fragments arranged by size:
3762 1071 975 85
BsmI GAATG_Cn'
Cuts at: 2730 3404 3497 2730
Size: 674 93 5126
Fragments arranged by size:
5126 674 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2105 2183 2307 2444 2608 2979
Size: 399 254 61 78 124 137 164 371
Cuts at: 2979 3212 3954 4039 5110 1391
Size: 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 254 233 164
137 124 85 78 61
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 3248 844
Size: 1289 1115 3489
Fragments arranged by size:
3489 1289 1115
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3118 3780 1394
Size: 429 260 1035 662 3507
Fragments arranged by size:
3507 1035 662 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4388 4562 65
Size: 1183 3140 174 1396
Fragments arranged by size:
3140 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 4537 4724 1700
Size: 110 307 2420 187 2869
Fragments arranged by size:
2869 2420 307 187 110
BsrGI T'GTAC_A
Cuts at: 96 3045 96
Size: 2949 2944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 2007 4697 2007
Size: 2690 3203
BstEII G'GTnAC_C
Cuts at: 1184 2120 1184
Size: 936 4957
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2889 3223 3590 3975 3992 5444 5530 5542
Size: 917 334 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 917 385 367 346 334 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3024 3024
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4384
Fragments arranged by size:
4384 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 3096 3409 4203 4223 2825
Size: 271 313 794 20 4495
Fragments arranged by size:
4495 794 313 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2115 3055
Size: 54 1425 50 275 18 215 70 940
Cuts at: 3055 3340 4229 5858 8
Size: 285 889 1629 43
Fragments arranged by size:
1629 1425 940 889 285 275 215 70
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 2115 3340 1537
Size: 275 233 70 1225 4090
Fragments arranged by size:
4090 1225 275 233 70
EarI CTCTTCn'nnn_
Cuts at: 1353 2993 3823 1353
Size: 1640 830 3423
Fragments arranged by size:
3423 1640 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2977 2977
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2321 2515 2798 2903 3038 3110
Size: 393 662 27 194 283 105 135 72
Cuts at: 3110 3956 4553 5340 5608 1239
Size: 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 662 597 393 283 268
194 135 105 72 27
EcoO109I rG'GnC_Cy
Cuts at: 2110 3121 3162 2110
Size: 1011 41 4841
Fragments arranged by size:
4841 1011 41
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FspI TGC'GCA
Cuts at: 2741 4399 2741
Size: 1658 4235
HaeII r_GCGC'y
Cuts at: 1678 2031 2152 5040 1678
Size: 353 121 2888 2531
Fragments arranged by size:
2888 2531 353 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2918 2950 4534 4566 4608
Size: 32 135 32 1346 32 1584 32 42
Cuts at: 4608 4640 1373
Size: 32 2626
Fragments arranged by size:
2626 1584 1346 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3040 3237 3483 669
Size: 988 281 1102 197 246 3079
Fragments arranged by size:
3079 1102 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KasI G'GCGC_C
Cuts at: 2027 2027
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 3004 5007 5191 1305
Size: 1182 517 2003 184 2007
Fragments arranged by size:
2007 2003 1182 517 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3851 4210 4369 518
Size: 648 2685 359 159 2042
Fragments arranged by size:
2685 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2846 3309 3986 5134 5379 1606
Size: 444 796 463 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 463 444 245
NarI GG'CG_CC
Cuts at: 2028 2028
Size: 5893
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4384
Fragments arranged by size:
4384 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3251 4796 1132
Size: 97 2022 1545 2229
Fragments arranged by size:
2229 2022 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2370 1108
Size: 1262 4631
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 3121 2110
Size: 1011 4882
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2794 3245 830
Size: 1964 451 3478
Fragments arranged by size:
3478 1964 451
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacI G_AGCT'C
Cuts at: 2979 2979
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3038 3235 1936
Size: 1102 197 4594
Fragments arranged by size:
4594 1102 197
SanDI GG'GwC_CC
Cuts at: 2110 2110
Size: 5893
SapI GCTCTTCn'nnn_
Cuts at: 2993 2993
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 2291 4141 5853 2291
Size: 1850 1712 2331
Fragments arranged by size:
2331 1850 1712
SfcI C'TryA_G
Cuts at: 826 1045 2542 2645 2790 2830 3241 4376 5057
Size: 219 1497 103 145 40 411 1135 681
Cuts at: 5057 5248 826
Size: 191 1471
Fragments arranged by size:
1497 1471 1135 681 411 219 191 145
103 40
SfoI GGC'GCC
Cuts at: 2029 2029
Size: 5893
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 2088 3220 1872
Size: 216 1132 4545
Fragments arranged by size:
4545 1132 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3997 4898 5160 820
Size: 197 926 2054 901 262 1553
Fragments arranged by size:
2054 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3190 3684 493
Size: 2697 494 2702
Fragments arranged by size:
2702 2697 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2022 2214 2228 3332 513
Size: 552 957 192 14 1104 3074
Fragments arranged by size:
3074 1104 957 552 192 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2377 2381 2902 2926 3920 3937 4090
Size: 1255 797 4 521 24 994 17 153
Cuts at: 4090 4275 4720 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 994 797 521 445 185 153
24 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 2652 3045
Size: 274 80 33 51 1443 312 363 393
Cuts at: 3045 4139 5851 96
Size: 1094 1712 138
Fragments arranged by size:
1712 1443 1094 393 363 312 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 2086 3218 1870
Size: 216 1132 4545
Fragments arranged by size:
4545 1132 216
XmnI GAAnn'nnTTC
Cuts at: 2291 4022 2291
Size: 1731 4162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI
SfiI SrfI SwaI XcmI