Restriction map for
pFN21ASDA0174
(Circular) (Six-base) MAPSORT of: pFN21ASDA0174.seq Check: 7843 from: 1 to: 5821
pFN21ASDA0174 5821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2324 3164 1937
Size: 387 840 4594
Fragments arranged by size:
4594 840 387
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3078 3884 5268 5536 1632
Size: 1446 806 1384 268 1917
Fragments arranged by size:
1917 1446 1384 806 268
AfeI AGC'GCT
Cuts at: 2051 2051
Size: 5821
AflII C'TTAA_G
Cuts at: 820 1017 2053 820
Size: 197 1036 4588
Fragments arranged by size:
4588 1036 197
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3056 3564 5136 3056
Size: 508 1572 3741
Fragments arranged by size:
3741 1572 508
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2062 3130 3356 3508 5617 1493
Size: 569 1068 226 152 2109 1697
Fragments arranged by size:
2109 1697 1068 569 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2880 4375 160
Size: 872 1848 1495 1606
Fragments arranged by size:
1848 1606 1495 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 2181 3151 3518 2181
Size: 970 367 4484
Fragments arranged by size:
4484 970 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 3038 3142 4597 618
Size: 325 217 627 917 334 104 1455 1842
Fragments arranged by size:
1842 1455 917 627 334 325 217 104
BanII G_rGCy'C
Cuts at: 1391 2033 2179 3140 1391
Size: 642 146 961 4072
Fragments arranged by size:
4072 961 642 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 3066 928
Size: 971 315 852 3683
Fragments arranged by size:
3683 971 852 315
BbvCI CC'TCA_GC
Cuts at: 3049 3049
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 4983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2602 5816 2602
Size: 3214 2607
BlpI GC'TnA_GC
Cuts at: 2046 3238 2046
Size: 1192 4629
Bme1580I G_kGCm'C
Cuts at: 1790 3882 5038 1790
Size: 2092 1156 2573
Fragments arranged by size:
2573 2092 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2336 4481 1239
Size: 1019 78 2145 2579
Fragments arranged by size:
2579 2145 1019 78
Bpu10I CC'TnA_GC
Cuts at: 3049 3049
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3118 3612 493
Size: 711 1914 494 2702
Fragments arranged by size:
2702 1914 711 494
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2334 2574 2670 1186
Size: 1148 240 96 4337
Fragments arranged by size:
4337 1148 240 96
BseYI C'CCAG_C
Cuts at: 2026 2159 5024 5520 2026
Size: 133 2865 496 2327
Fragments arranged by size:
2865 2327 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2889 1163
Size: 573 1153 4095
Fragments arranged by size:
4095 1153 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2179 2196 3882 3967 5038 2179
Size: 17 1686 85 1071 2962
Fragments arranged by size:
2962 1686 1071 85 17
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2179 2196 3140 3882 3967 5038
Size: 399 243 146 17 944 742 85 1071
Cuts at: 5038 1391
Size: 2174
Fragments arranged by size:
2174 1071 944 742 399 243 146 85
17
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2262 2971 3176 844
Size: 1418 709 205 3489
Fragments arranged by size:
3489 1418 709 205
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 2216 3881 4893 2216
Size: 1665 1012 3144
Fragments arranged by size:
3144 1665 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 2007 4625 2007
Size: 2618 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2503 2503
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2181 2232 2602 3151 3518 3903 3920 5372
Size: 209 51 370 549 367 385 17 1452
Cuts at: 5372 5458 5470 5816 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 549 385 370 367 346 209
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2226 513
Size: 552 1161 4108
Fragments arranged by size:
4108 1161 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2759 3337 4131 4151 2759
Size: 578 794 20 4429
Fragments arranged by size:
4429 794 578 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 2041 3125 2041
Size: 1084 4737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2567 2984 4828 809
Size: 507 1251 417 1844 1802
Fragments arranged by size:
1844 1802 1251 507 417
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786
Size: 54 1425 50 275 18 1438 889 1629
Cuts at: 5786 8
Size: 43
Fragments arranged by size:
1629 1438 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2647 2905 3090 3751 1353
Size: 1294 258 185 661 3423
Fragments arranged by size:
3423 1294 661 258 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2177 2177
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2336 3078 3884 4481 5268 5536
Size: 393 626 78 742 806 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 806 787 742 626 597 393 268
78
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 2652 2219
Size: 433 5388
EcoO109I rG'GnC_Cy
Cuts at: 2645 2645
Size: 5821
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2053 4968 1678
Size: 375 2915 2531
Fragments arranged by size:
2915 2531 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2902 2934 2987
Size: 32 135 32 594 32 704 32 53
Cuts at: 2987 3019 4462 4494 4536 4568 1373
Size: 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 704 594 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2948 748
Size: 2200 3621
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2596 4935 5119 1305
Size: 1291 2339 184 2007
Fragments arranged by size:
2339 2007 1291 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 2726 3779 4138 4297 518
Size: 648 1554 6 1053 359 159 2042
Fragments arranged by size:
2042 1554 1053 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 3237 3914 5062 5307 1606
Size: 1631 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2226 513
Size: 552 1161 4108
Fragments arranged by size:
4108 1161 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2687 1108
Size: 1579 4242
PfoI T'CCnGG_A
Cuts at: 2202 2235 2920 3083 2202
Size: 33 685 163 4940
Fragments arranged by size:
4940 685 163 33
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2353 1613
Size: 740 5081
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2968 3173 830
Size: 2138 205 3478
Fragments arranged by size:
3478 2138 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SacI G_AGCT'C
Cuts at: 2179 2179
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 3090 3090
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2868 2964 3169 4304 4985 5176 826
Size: 219 1823 96 205 1135 681 191 1471
Fragments arranged by size:
1823 1471 1135 681 219 205 191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3925 4826 5088 820
Size: 197 926 110 1872 901 262 1553
Fragments arranged by size:
1872 1553 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 3118 3612 493
Size: 2625 494 2702
Fragments arranged by size:
2702 2625 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 2824 3745 5
Size: 47 2772 921 2081
Fragments arranged by size:
2772 2081 921 47
StuI AGG'CCT
Cuts at: 1517 2813 1517
Size: 1296 4525
StyI C'CwwG_G
Cuts at: 513 1065 2226 3260 513
Size: 552 1161 1034 3074
Fragments arranged by size:
3074 1161 1034 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 3848 3865 4018 4203 4648 325
Size: 1255 929 1339 17 153 185 445 1498
Fragments arranged by size:
1498 1339 1255 929 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 4067 5779
Size: 274 80 33 51 1443 267 1823 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1823 1712 1443 274 267 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 5781 5813 2484
Size: 32 3265 32 2492
Fragments arranged by size:
3265 2492 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2233 2503 2233
Size: 270 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI