Restriction map for
pFN21ASDA0170
(Circular) (Six-base) MAPSORT of: pFN21ASDA0170.seq Check: 5674 from: 1 to: 10996
pFN21ASDA0170 10996 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5068 5068
Size: 10996
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8806 278
Size: 53 83 186 936 7270 2468
Fragments arranged by size:
7270 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4468 8339 1937
Size: 2531 3871 4594
Fragments arranged by size:
4594 3871 2531
Acc65I G'GTAC_C
Cuts at: 4244 8317 4244
Size: 4073 6923
AclI AA'CG_TT
Cuts at: 1563 9123 9496 1563
Size: 7560 373 3063
Fragments arranged by size:
7560 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2787 2793 4095 4116 5172 6740 8252 9059
Size: 1155 6 1302 21 1056 1568 1512 807
Cuts at: 9059 10443 10711 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1568 1512 1384 1302 1155 1056 807
268 21 6
AfeI AGC'GCT
Cuts at: 3261 3261
Size: 10996
AflII C'TTAA_G
Cuts at: 820 1017 3866 820
Size: 197 2849 7950
Fragments arranged by size:
7950 2849 197
AflIII A'CryG_T
Cuts at: 1203 3334 4051 9895 10872 1203
Size: 2131 717 5844 977 1327
Fragments arranged by size:
5844 2131 1327 977 717
AgeI A'CCGG_T
Cuts at: 9827 9827
Size: 10996
AhdI GACnn_n'nnGTC
Cuts at: 9725 9725
Size: 10996
AleI CACnn'nnGTG
Cuts at: 1166 2684 2927 3389 3575 3593 7718 1166
Size: 1518 243 462 186 18 4125 4444
Fragments arranged by size:
4444 4125 1518 462 243 186 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10996
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5736 5768 7199 7231 5490
Size: 32 214 32 1431 32 9255
Fragments arranged by size:
9255 1431 214 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4144 4928 5150 5519 5765 5888 6011 6380 6503
Size: 784 222 369 246 123 123 369 123
Cuts at: 6503 6626 6872 6995 8739 10311 4144
Size: 123 246 123 1744 1572 4829
Fragments arranged by size:
4829 1744 1572 784 369 369 246 246
222 123 123 123 123 123
ApaI G_GGCC'C
Cuts at: 2453 3898 4499 2453
Size: 1445 601 8950
Fragments arranged by size:
8950 1445 601
ApaLI G'TGCA_C
Cuts at: 9053 10209 9053
Size: 1156 9840
ApoI r'AATT_y
Cuts at: 1493 7118 8305 8531 8683 10792 1493
Size: 5625 1187 226 152 2109 1697
Fragments arranged by size:
5625 2109 1697 1187 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9550 160
Size: 872 8518 1606
Fragments arranged by size:
8518 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10996
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2445 6189 6263 6681 6804 7731
Size: 73 231 271 3744 74 418 123 927
Cuts at: 7731 7992 8321 1870
Size: 261 329 4545
Fragments arranged by size:
4545 3744 927 418 329 271 261 231
123 74 73
AvrII C'CTAG_G
Cuts at: 4367 4367
Size: 10996
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2826 2859 3144 3177 2826
Size: 33 285 33 10645
Fragments arranged by size:
10645 285 33 33
BamHI G'GATC_C
Cuts at: 3033 3543 5003 8326 8693 3033
Size: 510 1460 3323 367 5336
Fragments arranged by size:
5336 3323 1460 510 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2278 2741 3773 4244
Size: 325 217 627 351 140 463 1032 471
Cuts at: 4244 4831 8317 9772 618
Size: 587 3486 1455 1842
Fragments arranged by size:
3486 1842 1455 1032 627 587 471 463
351 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2348 2453 2986 3408 3460 3536 3883 3898
Size: 957 105 533 422 52 76 347 15
Cuts at: 3898 4021 4403 4499 5018 5403 5526 5568 5649
Size: 123 382 96 519 385 123 42 81
Cuts at: 5649 5772 5814 5895 6018 6141 6183 6306 6387
Size: 123 42 81 123 123 42 123 81
Cuts at: 6387 6552 6633 6675 6798 6879 7431 7641 7993
Size: 165 81 42 123 81 552 210 352
Cuts at: 7993 8315 1391
Size: 322 4072
Fragments arranged by size:
4072 957 552 533 519 422 385 382
352 347 322 210 165 123 123 123
123 123 123 123 105 96 81 81
81 81 81 76 52 42 42 42
42 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4083 6356 7071 7532 928
Size: 971 2184 2273 715 461 4392
Fragments arranged by size:
4392 2273 2184 971 715 461
BbvCI CC'TCA_GC
Cuts at: 2517 2725 2964 4963 2517
Size: 208 239 1999 8550
Fragments arranged by size:
8550 1999 239 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2999 3033 9187 9221 1903
Size: 34 1062 34 6154 34 3678
Fragments arranged by size:
6154 3678 1062 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8888 10098 8888
Size: 1210 9786
BclI T'GATC_A
Cuts at: 1548 8139 1548
Size: 6591 4405
BfrBI ATG'CAT
Cuts at: 3631 3631
Size: 10996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2116 3182 9607
Size: 107 122 71 631 605 444 1066 6425
Cuts at: 9607 136
Size: 1525
Fragments arranged by size:
6425 1525 1066 631 605 444 122 107
71
BglII A'GATC_T
Cuts at: 3075 3961 4099 7409 8022 10991 3075
Size: 886 138 3310 613 2969 3080
Fragments arranged by size:
3310 3080 2969 886 613 138
BlpI GC'TnA_GC
Cuts at: 2985 8413 2985
Size: 5428 5568
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2453 3776 3898 4499 4836 5634 5880
Size: 353 310 1323 122 601 337 798 246
Cuts at: 5880 6618 7843 9057 10213 1790
Size: 738 1225 1214 1156 2573
Fragments arranged by size:
2573 1323 1225 1214 1156 798 738 601
353 337 310 246 122
BmgBI CAC'GTC
Cuts at: 1318 3391 1318
Size: 2073 8923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3041 3404
Size: 416 53 83 399 24 639 985 363
Cuts at: 3404 4114 4397 5003 7170 7766 8727 9685 442
Size: 710 283 606 2167 596 961 958 1753
Fragments arranged by size:
2167 1753 985 961 958 710 639 606
596 416 399 363 283 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10996
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2740 2919 3793 3971 7360 8007 2737
Size: 3 179 874 178 3389 647 5726
Fragments arranged by size:
5726 3389 874 647 179 178 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2909 2928 3126 3215 3572 4151 4868 4894
Size: 1670 19 198 89 357 579 717 26
Cuts at: 4894 5106 5274 5336 5344 5510 5633 5756 6002
Size: 212 168 62 8 166 123 123 246
Cuts at: 6002 6371 6617 6754 7415 8058 8244 9656 1239
Size: 369 246 137 661 643 186 1412 2579
Fragments arranged by size:
2579 1670 1412 717 661 643 579 369
357 246 246 212 198 186 168 166
137 123 123 89 62 26 19 8
Bpu10I CC'TnA_GC
Cuts at: 2517 2725 2964 4963 5397 5520 5766 5889 6012
Size: 208 239 1999 434 123 246 123 123
Cuts at: 6012 6054 6135 6177 6258 6300 6381 6423 6504
Size: 42 81 42 81 42 81 42 81
Cuts at: 6504 6627 6669 6750 6792 6849 6873 7050 7158
Size: 123 42 81 42 57 24 177 108
Cuts at: 7158 7335 7941 2517
Size: 177 606 5572
Fragments arranged by size:
5572 1999 606 434 246 239 208 177
177 123 123 123 123 108 81 81
81 81 81 57 42 42 42 42
42 42 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2113 3419 3928 4358 5663 7022 7264 7379 9121
Size: 1306 509 430 1305 1359 242 115 1742
Cuts at: 9121 9986 10284 2113
Size: 865 298 2825
Fragments arranged by size:
2825 1742 1359 1306 1305 865 509 430
298 242 115
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2320 4307 4377 4777 7614 9659 882
Size: 617 821 1987 70 400 2837 2045 2219
Fragments arranged by size:
2837 2219 2045 1987 821 617 400 70
BsaAI yAC'GTr
Cuts at: 493 1204 8293 8787 493
Size: 711 7089 494 2702
Fragments arranged by size:
7089 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2258 10990 2258
Size: 8732 2264
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8803 9185 9820 275
Size: 53 83 186 936 7270 382 635 1451
Fragments arranged by size:
7270 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 9429 9827 10101 10248 10659 9429
Size: 398 274 147 411 9766
Fragments arranged by size:
9766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 4008 4038 7199
Size: 30 165 30 868 30 1707 30 3161
Cuts at: 7199 7229 1178
Size: 30 4945
Fragments arranged by size:
4945 3161 1707 868 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2081 2543 2713 2729 2908 3410 3782
Size: 892 3 462 170 16 179 502 372
Cuts at: 3782 7646 7649 8189 1186
Size: 3864 3 540 3993
Fragments arranged by size:
3993 3864 892 540 502 462 372 179
170 16 3 3
BseYI C'CCAG_C
Cuts at: 2876 3037 3839 3999 4317 5158 5424 7202 7350
Size: 161 802 160 318 841 266 1778 148
Cuts at: 7350 8102 10199 10695 2876
Size: 752 2097 496 3177
Fragments arranged by size:
3177 2097 1778 841 802 752 496 318
266 161 160 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2874 6838 7236 1163
Size: 573 1138 3964 398 4923
Fragments arranged by size:
4923 3964 1138 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5671 8446 9207 10235 1540
Size: 275 186 3670 2775 761 1028 2301
Fragments arranged by size:
3670 2775 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3112 3408 3472 3536 3883 5526 5649 5772 5895
Size: 296 64 64 347 1643 123 123 123
Cuts at: 5895 6018 6141 6387 6633 7431 7710 9057 9142
Size: 123 123 246 246 798 279 1347 85
Cuts at: 9142 10213 3112
Size: 1071 3895
Fragments arranged by size:
3895 1643 1347 1071 798 347 296 279
246 246 123 123 123 123 123 85
64 64
BsmI GAATG_Cn'
Cuts at: 2202 2623 8507 8600 2202
Size: 421 5884 93 4598
Fragments arranged by size:
5884 4598 421 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6438 6438
Size: 10996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2348 2453 2986 3112 3408 3460
Size: 399 353 205 105 533 126 296 52
Cuts at: 3460 3472 3536 3776 3883 3898 4021 4403 4499
Size: 12 64 240 107 15 123 382 96
Cuts at: 4499 4836 5018 5403 5526 5568 5634 5649 5772
Size: 337 182 385 123 42 66 15 123
Cuts at: 5772 5814 5880 5895 6018 6141 6183 6306 6387
Size: 42 66 15 123 123 42 123 81
Cuts at: 6387 6552 6618 6633 6675 6798 6879 7431 7641
Size: 165 66 15 42 123 81 552 210
Cuts at: 7641 7710 7843 7993 8315 9057 9142 10213 1391
Size: 69 133 150 322 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 533 399 385 382
353 337 322 296 240 210 205 182
165 150 133 126 123 123 123 123
123 123 123 107 105 96 85 81
81 69 66 66 66 64 52 42
42 42 42 15 15 15 15 12
BspEI T'CCGG_A
Cuts at: 10659 10659
Size: 10996
BspHI T'CATG_A
Cuts at: 3883 8885 10615 3883
Size: 5002 1730 4264
Fragments arranged by size:
5002 4264 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5068 7394 8351 844
Size: 4224 2326 957 3489
Fragments arranged by size:
4224 3489 2326 957
BsrBI CCG'CTC
Cuts at: 1394 1823 7741 8883 1394
Size: 429 5918 1142 3507
Fragments arranged by size:
5918 3507 1142 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4222 8164 9491 9665 65
Size: 1183 2974 3942 1327 174 1396
Fragments arranged by size:
3942 2974 1396 1327 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7099 9640 9827 1700
Size: 110 5289 2541 187 2869
Fragments arranged by size:
5289 2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10996
BssSI C'ACGA_G
Cuts at: 9056 10068 9056
Size: 1012 9984
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10996
BstBI TT'CG_AA
Cuts at: 2007 9800 2007
Size: 7793 3203
BstEII G'GTnAC_C
Cuts at: 1184 7930 1184
Size: 6746 4250
BstXI CCAn_nnnn'nTGG
Cuts at: 2906 3577 4211 4994 2906
Size: 671 634 783 8908
Fragments arranged by size:
8908 783 671 634
BstYI r'GATC_y
Cuts at: 1972 3033 3075 3543 3961 4099 5003 5600 5723
Size: 1061 42 468 418 138 904 597 123
Cuts at: 5723 5846 6215 6461 7383 7409 8022 8326 8693
Size: 123 369 246 922 26 613 304 367
Cuts at: 8693 9078 9095 10547 10633 10645 10991 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1061 922 904 613 597 468
418 385 369 367 346 304 246 138
123 123 86 42 26 17 12
BstZ17I GTA'TAC
Cuts at: 4469 4469
Size: 10996
Bsu36I CC'TnA_GG
Cuts at: 2334 7038 7437 8151 2334
Size: 4704 399 714 5179
Fragments arranged by size:
5179 4704 714 399
BtgI C'CryG_G
Cuts at: 513 1065 2022 2143 3082 3213 4259 7473 513
Size: 552 957 121 939 131 1046 3214 4036
Fragments arranged by size:
4036 3214 1046 957 939 552 131 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2399 487
Size: 683 56 1173 9084
Fragments arranged by size:
9084 1173 683 56
BtsI GCAGTG_nn'
Cuts at: 7308 8512 9306 9326 7308
Size: 1204 794 20 8978
Fragments arranged by size:
8978 1204 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5623 5658 529
Size: 35 5059 35 5867
Fragments arranged by size:
5867 5059 35 35
DraI TTT'AAA
Cuts at: 8300 8300
Size: 10996
DraIII CAC_nnn'GTG
Cuts at: 6816 6816
Size: 10996
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 5552 5675 5798 5921 5968 6044
Size: 507 4160 76 123 123 123 47 76
Cuts at: 6044 6091 6290 6337 6536 6659 6706 6829 6905
Size: 47 199 47 199 123 47 123 76
Cuts at: 6905 6952 7028 10003 809
Size: 47 76 2975 1802
Fragments arranged by size:
4160 2975 1802 507 199 199 123 123
123 123 123 76 76 76 76 47
47 47 47 47
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 8443 9332 10961
Size: 54 1425 50 275 18 6613 889 1629
Cuts at: 10961 8
Size: 43
Fragments arranged by size:
6613 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 8443 1537
Size: 275 6631 4090
Fragments arranged by size:
6631 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2460 2610 2731 3021 3062 3312 3396
Size: 702 405 150 121 290 41 250 84
Cuts at: 3396 4818 7539 7575 7715 8926 1353
Size: 1422 2721 36 140 1211 3423
Fragments arranged by size:
3423 2721 1422 1211 702 405 290 250
150 140 121 84 41 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5202 6508 7805 9583 9957 10103
Size: 1064 138 3613 1306 1297 1778 374 146
Cuts at: 10103 387
Size: 1280
Fragments arranged by size:
3613 1778 1306 1297 1280 1064 374 146
138
EcoICRI GAG'CTC
Cuts at: 3406 3534 3881 5524 5647 5770 5893 6016 6139
Size: 128 347 1643 123 123 123 123 123
Cuts at: 6139 6385 6631 7429 3406
Size: 246 246 798 6973
Fragments arranged by size:
6973 1643 798 347 246 246 128 123
123 123 123 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2787 2793 2909 2928 3126 3215 3572
Size: 393 1155 6 116 19 198 89 357
Cuts at: 3572 4095 4116 4151 4868 4894 5106 5172 5274
Size: 523 21 35 717 26 212 66 102
Cuts at: 5274 5336 5344 5510 5633 5756 6002 6371 6617
Size: 62 8 166 123 123 246 369 246
Cuts at: 6617 6740 6754 7415 8058 8244 8252 9059 9656
Size: 123 14 661 643 186 8 807 597
Cuts at: 9656 10443 10711 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1155 807 787 717 661 643 597
523 393 369 357 268 246 246 212
198 186 166 123 123 123 116 102
89 66 62 35 26 21 19 14
8 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4503 4503
Size: 10996
EcoO109I rG'GnC_Cy
Cuts at: 2449 2450 3101 3175 3894 3931 4024 4141 4267
Size: 1 651 74 719 37 93 117 126
Cuts at: 4267 4496 4945 4955 5478 6339 6708 6831 6954
Size: 229 449 10 523 861 369 123 123
Cuts at: 6954 2449
Size: 6491
Fragments arranged by size:
6491 861 719 651 523 449 369 229
126 123 123 117 93 74 37 10
1
EcoRI G'AATT_C
Cuts at: 7118 8305 8683 7118
Size: 1187 378 9431
Fragments arranged by size:
9431 1187 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3225 3225
Size: 10996
FspI TGC'GCA
Cuts at: 9502 9502
Size: 10996
HaeII r_GCGC'y
Cuts at: 1678 3263 10143 1678
Size: 1585 6880 2531
Fragments arranged by size:
6880 2531 1585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 3414 3446 3518
Size: 32 135 32 775 32 1035 32 72
Cuts at: 3518 3550 4008 4040 5102 5134 5555 5587 5592
Size: 32 458 32 1062 32 421 32 5
Cuts at: 5592 5624 5715 5747 5838 5870 6170 6202 6293
Size: 32 91 32 91 32 300 32 91
Cuts at: 6293 6325 6416 6448 6449 6453 6481 6485 6539
Size: 32 91 32 1 4 28 4 54
Cuts at: 6539 6571 6662 6694 6785 6817 7500 7532 8124
Size: 32 91 32 91 32 683 32 592
Cuts at: 8124 8156 8267 8299 9637 9669 9711 9743 1373
Size: 32 111 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1338 1062 1035 775 683 592 458
421 300 135 111 91 91 91 91
91 91 72 54 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 28 5 4 4 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4194 8340 8586 669
Size: 988 281 2256 4146 246 3079
Fragments arranged by size:
4146 3079 2256 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3207 748
Size: 2459 8537
HpaI GTT'AAC
Cuts at: 8586 8586
Size: 10996
KpnI G_GTAC'C
Cuts at: 4248 8321 4248
Size: 4073 6923
MfeI C'AATT_G
Cuts at: 6732 8595 6732
Size: 1863 9133
MluI A'CGCG_T
Cuts at: 10872 10872
Size: 10996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7690 10110 10294 1305
Size: 6385 2420 184 2007
Fragments arranged by size:
6385 2420 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 9174
Fragments arranged by size:
9174 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2927 3059 3389 3575 3593 4056
Size: 648 1518 243 132 330 186 18 463
Cuts at: 4056 7718 8954 9313 9472 518
Size: 3662 1236 359 159 2042
Fragments arranged by size:
3662 2042 1518 1236 648 463 359 330
243 186 159 132 18
MspA1I CmG'CkG
Cuts at: 1606 2729 2876 2963 3860 7202 7313 7781 8412
Size: 1123 147 87 897 3342 111 468 631
Cuts at: 8412 9089 10237 10482 1606
Size: 677 1148 245 2120
Fragments arranged by size:
3342 2120 1148 1123 897 677 631 468
245 147 111 87
NcoI C'CATG_G
Cuts at: 513 1065 2022 2143 3082 3213 4259 7473 513
Size: 552 957 121 939 131 1046 3214 4036
Fragments arranged by size:
4036 3214 1046 957 939 552 131 121
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10996
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10996
NotI GC'GGCC_GC
Cuts at: 8443 8443
Size: 10996
NsiI A_TGCA'T
Cuts at: 3633 3633
Size: 10996
NspI r_CATG'y
Cuts at: 1132 1229 2249 3328 4055 8110 8148 8354 9899
Size: 97 1020 1079 727 4055 38 206 1545
Cuts at: 9899 1132
Size: 2229
Fragments arranged by size:
4055 2229 1545 1079 1020 727 206 97
38
PasI CC'CwG_GG
Cuts at: 1348 2348 3898 7843 1348
Size: 1000 1550 3945 4501
Fragments arranged by size:
4501 3945 1550 1000
PciI A'CATG_T
Cuts at: 4051 9895 4051
Size: 5844 5152
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 3337 4162 4928 7393 1108
Size: 1311 918 825 766 2465 4711
Fragments arranged by size:
4711 2465 1311 918 825 766
PfoI T'CCnGG_A
Cuts at: 2905 2950 3103 4845 4951 5075 2905
Size: 45 153 1742 106 124 8826
Fragments arranged by size:
8826 1742 153 124 106 45
PmeI GTTT'AAAC
Cuts at: 8300 8300
Size: 10996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7199 7231 9065 9097 10578 10610 7199
Size: 32 1834 32 1481 32 7585
Fragments arranged by size:
7585 1834 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3101 3931 4024 4267 4945 4955 5478 6339 6708
Size: 830 93 243 678 10 523 861 369
Cuts at: 6708 6831 6954 3101
Size: 123 123 7143
Fragments arranged by size:
7143 861 830 678 523 369 243 123
123 93 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10996
PsiI TTA'TAA
Cuts at: 8566 8566
Size: 10996
PspOMI G'GGCC_C
Cuts at: 2449 3894 4495 2449
Size: 1445 601 8950
Fragments arranged by size:
8950 1445 601
PspXI vC'TCGA_Gb
Cuts at: 6263 6263
Size: 10996
PstI C_TGCA'G
Cuts at: 830 3132 3351 3859 5146 7564 8348 830
Size: 2302 219 508 1287 2418 784 3478
Fragments arranged by size:
3478 2418 2302 1287 784 508 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10996
PvuII CAG'CTG
Cuts at: 2729 2876 2963 3860 7313 2729
Size: 147 87 897 3453 6412
Fragments arranged by size:
6412 3453 897 147 87
SacI G_AGCT'C
Cuts at: 3408 3536 3883 5526 5649 5772 5895 6018 6141
Size: 128 347 1643 123 123 123 123 123
Cuts at: 6141 6387 6633 7431 3408
Size: 246 246 798 6973
Fragments arranged by size:
6973 1643 798 347 246 246 128 123
123 123 123 123
SalI G'TCGA_C
Cuts at: 1936 8338 1936
Size: 6402 4594
SapI GCTCTTCn'nnn_
Cuts at: 3396 7715 3396
Size: 4319 6677
SbfI CC_TGCA'GG
Cuts at: 8348 8348
Size: 10996
ScaI AGT'ACT
Cuts at: 9244 10956 9244
Size: 1712 9284
SexAI A'CCwGG_T
Cuts at: 7797 7797
Size: 10996
SfcI C'TryA_G
Cuts at: 826 1045 2748 3128 3347 3855 5142 6126 6509
Size: 219 1703 380 219 508 1287 984 383
Cuts at: 6509 6741 7560 8344 9479 10160 10351 826
Size: 232 819 784 1135 681 191 1471
Fragments arranged by size:
1703 1471 1287 1135 984 819 784 681
508 383 380 232 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10996
SmaI CCC'GGG
Cuts at: 1872 2447 8323 1872
Size: 575 5876 4545
Fragments arranged by size:
5876 4545 575
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2092 3434 3866 3943 4337 5642
Size: 197 926 149 1342 432 77 394 1305
Cuts at: 5642 6263 7001 7279 7358 9100 10001 10263 820
Size: 621 738 278 79 1742 901 262 1553
Fragments arranged by size:
1742 1553 1342 1305 926 901 738 621
432 394 278 262 197 149 79 77
SnaBI TAC'GTA
Cuts at: 493 8293 8787 493
Size: 7800 494 2702
Fragments arranged by size:
7800 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10996
SphI G_CATG'C
Cuts at: 1132 8110 8354 1132
Size: 6978 244 3774
Fragments arranged by size:
6978 3774 244
SspI AAT'ATT
Cuts at: 5 52 8920 5
Size: 47 8868 2081
Fragments arranged by size:
8868 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 2947 3117 4184 4343 4907 5037 7277
Size: 1203 227 170 1067 159 564 130 2240
Cuts at: 7277 7442 8255 1517
Size: 165 813 4258
Fragments arranged by size:
4258 2240 1203 1067 813 564 227 170
165 159 130
StyI C'CwwG_G
Cuts at: 513 1065 2022 2143 2644 3082 3213 4065 4259
Size: 552 957 121 501 438 131 852 194
Cuts at: 4259 4367 4581 7257 7473 8435 513
Size: 108 214 2676 216 962 3074
Fragments arranged by size:
3074 2676 962 957 852 552 501 438
216 214 194 131 121 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2488 4013 4046 4643 5343 5685
Size: 1255 468 440 1525 33 597 700 342
Cuts at: 5685 5931 6573 6669 7949 8103 9023 9040 9193
Size: 246 642 96 1280 154 920 17 153
Cuts at: 9193 9378 9823 325
Size: 185 445 1498
Fragments arranged by size:
1525 1498 1280 1255 920 700 642 597
468 445 440 342 246 185 154 153
96 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2479 4735 9242
Size: 274 80 33 51 1443 502 2256 4507
Cuts at: 9242 10954 96
Size: 1712 138
Fragments arranged by size:
4507 2256 1712 1443 502 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2271 2303 3171 3203 3528 3560 4995 5027 10956
Size: 32 868 32 325 32 1435 32 5929
Cuts at: 10956 10988 2271
Size: 32 2279
Fragments arranged by size:
5929 2279 1435 868 325 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10996
XbaI T'CTAG_A
Cuts at: 3721 8332 3721
Size: 4611 6385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3494 4843 7867 3494
Size: 1349 3024 6623
Fragments arranged by size:
6623 3024 1349
XhoI C'TCGA_G
Cuts at: 1943 6263 1943
Size: 4320 6676
XmaI C'CCGG_G
Cuts at: 1870 2445 8321 1870
Size: 575 5876 4545
Fragments arranged by size:
5876 4545 575
XmnI GAAnn'nnTTC
Cuts at: 2382 3077 9125 2382
Size: 695 6048 4253
Fragments arranged by size:
6048 4253 695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8804 276
Size: 53 83 186 936 7270 2468
Fragments arranged by size:
7270 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BsiWI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI