Restriction map for
pFN21ASDA0168
(Circular) (Six-base) MAPSORT of: pFN21ASDA0168.seq Check: 9360 from: 1 to: 5707
pFN21ASDA0168 5707 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2339 2432 2657 2747 3770 5154 5422
Size: 491 216 93 225 90 1023 1384 268
Cuts at: 5422 1632
Size: 1917
Fragments arranged by size:
1917 1384 1023 491 268 225 216 93
90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2635 2725 2968 3450 5022 2635
Size: 90 243 482 1572 3320
Fragments arranged by size:
3320 1572 482 243 90
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 5707
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2134 2654 2668 3016 3242 3394 5503 1493
Size: 641 520 14 348 226 152 2109 1697
Fragments arranged by size:
2109 1697 641 520 348 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2148 3028 4483 618
Size: 325 217 627 361 880 1455 1842
Fragments arranged by size:
1842 1455 880 627 361 325 217
BanII G_rGCy'C
Cuts at: 1391 2187 2305 2771 3026 1391
Size: 796 118 466 255 4072
Fragments arranged by size:
4072 796 466 255 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2111 2169 928
Size: 971 212 58 4466
Fragments arranged by size:
4466 971 212 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2346 2781 5702 2346
Size: 435 2921 2351
Fragments arranged by size:
2921 2351 435
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2337 2771 3768 4924 1790
Size: 547 434 997 1156 2573
Fragments arranged by size:
2573 1156 997 547 434
BmgBI CAC'GTC
Cuts at: 1318 2836 1318
Size: 1518 4189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2836 3438 4396
Size: 416 53 83 399 24 1419 602 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1419 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2386 2747 2876 2990 4367 1239
Size: 1147 361 129 114 1377 2579
Fragments arranged by size:
2579 1377 1147 361 129 114
Bpu10I CC'TnA_GC
Cuts at: 2304 2304
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2978 4370 882
Size: 617 1479 1392 2219
Fragments arranged by size:
2219 1479 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 2695 3004 3498 493
Size: 711 1491 309 494 2702
Fragments arranged by size:
2702 1491 711 494 309
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2492 2518 3514 3896
Size: 53 83 186 936 959 26 996 382
Cuts at: 3896 4531 275
Size: 635 1451
Fragments arranged by size:
1451 996 959 936 635 382 186 83
53 26
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 1178
Size: 30 165 30 1110 30 4342
Fragments arranged by size:
4342 1110 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2263 2693 2987 1186
Size: 1074 3 430 294 3906
Fragments arranged by size:
3906 1074 430 294 3
BseYI C'CCAG_C
Cuts at: 2989 4910 5406 2989
Size: 1921 496 3290
Fragments arranged by size:
3290 1921 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2946 3768 3853 4924 2946
Size: 822 85 1071 3729
Fragments arranged by size:
3729 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2250 3218 3311 2250
Size: 968 93 4646
Fragments arranged by size:
4646 968 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2305 2337 2771 2946 3026 3768
Size: 399 397 118 32 434 175 80 742
Cuts at: 3768 3853 4924 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 397 175 118
85 80 32
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2488 4202 4376 65
Size: 1183 1240 1714 174 1396
Fragments arranged by size:
1714 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 2863 4351 4538 1700
Size: 110 401 652 1488 187 2869
Fragments arranged by size:
2869 1488 652 401 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 2007 4511 2007
Size: 2504 3203
BstEII G'GTnAC_C
Cuts at: 1184 2375 1184
Size: 1191 4516
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 2708
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2346 2781 3037 3404 3789 3806 5258 5344
Size: 374 435 256 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 435 385 374 367 346 256
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2476 513
Size: 552 957 454 3744
Fragments arranged by size:
3744 957 552 454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 3223 4017 4037 2461
Size: 762 794 20 4131
Fragments arranged by size:
4131 794 762 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2456 2456
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 3154 4043
Size: 54 1425 50 275 18 709 615 889
Cuts at: 4043 5672 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 709 615 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2339 2386 2432 2657 2747 2876
Size: 393 491 216 47 46 225 90 129
Cuts at: 2876 2990 3770 4367 5154 5422 1239
Size: 114 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 597 491 393 268 225
216 129 114 90 47 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 2423
Size: 5707
EcoO109I rG'GnC_Cy
Cuts at: 2203 2407 2632 2767 2203
Size: 204 225 135 5143
Fragments arranged by size:
5143 225 204 135
EcoRI G'AATT_C
Cuts at: 2668 3016 3394 2668
Size: 348 378 4981
Fragments arranged by size:
4981 378 348
FspI TGC'GCA
Cuts at: 2447 2930 4213 2447
Size: 483 1283 3941
Fragments arranged by size:
3941 1283 483
FspAI rTGC'GCAy
Cuts at: 2447 2447
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2875 748
Size: 2127 3580
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 3665 4024 4183 518
Size: 648 1540 959 359 159 2042
Fragments arranged by size:
2042 1540 959 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 2506 2746 3123 3800 4948 5193 1606
Size: 834 66 240 377 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 377 245 240 66
NaeI GCC'GGC
Cuts at: 2213 2213
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4198
Fragments arranged by size:
4198 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2211 2211
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2582 1108
Size: 1474 4233
PfoI T'CCnGG_A
Cuts at: 2892 2892
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 5707
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 5675
PstI C_TGCA'G
Cuts at: 830 2229 2424 3059 830
Size: 1399 195 635 3478
Fragments arranged by size:
3478 1399 635 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2440 2440
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 2424 3059 2424
Size: 635 5072
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 5707
SfcI C'TryA_G
Cuts at: 826 1045 2029 2225 2420 3055 4190 4871 5062
Size: 219 984 196 195 635 1135 681 191
Cuts at: 5062 826
Size: 1471
Fragments arranged by size:
1471 1135 984 681 635 219 196 195
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3004 3498 493
Size: 2511 494 2702
Fragments arranged by size:
2702 2511 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2022 3146 513
Size: 552 957 1124 3074
Fragments arranged by size:
3074 1124 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2934 3953
Size: 274 80 33 51 1443 141 816 1019
Cuts at: 3953 5665 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 816 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 2482 2513 2514 2545 5667 5699 2027
Size: 32 423 31 1 31 3122 32 2035
Fragments arranged by size:
3122 2035 423 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2250 3836 2250
Size: 1586 4121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI KasI NarI NruI NsiI PacI PmlI PspXI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI