Restriction map for
pFN21ASDA0161
(Circular) (Six-base) MAPSORT of: pFN21ASDA0161.seq Check: 1073 from: 1 to: 5605
pFN21ASDA0161 5605 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2375 2465 2375
Size: 90 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 2948 1937
Size: 655 356 4594
Fragments arranged by size:
4594 655 356
Acc65I G'GTAC_C
Cuts at: 2037 2926 2037
Size: 889 4716
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3668 5052 5320 1632
Size: 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 268
AfeI AGC'GCT
Cuts at: 2573 2573
Size: 5605
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2230 1184
Size: 1046 4559
AlwNI CAG_nnn'CTG
Cuts at: 2225 3348 4920 2225
Size: 1123 1572 2910
Fragments arranged by size:
2910 1572 1123
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2444 2914 3140 3292 5401 1493
Size: 951 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 2739 2930 1870
Size: 73 576 220 191 4545
Fragments arranged by size:
4545 576 220 191 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2352 2472 2926 4381
Size: 325 217 627 250 315 120 454 1455
Cuts at: 4381 618
Size: 1842
Fragments arranged by size:
1842 1455 627 454 325 315 250 217
120
BanII G_rGCy'C
Cuts at: 1391 2181 2924 1391
Size: 790 743 4072
Fragments arranged by size:
4072 790 743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 2184 1548
Size: 636 4969
BfrBI ATG'CAT
Cuts at: 2142 2142
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2357 3666 4822 1790
Size: 567 1309 1156 2573
Fragments arranged by size:
2573 1309 1156 567
BmgBI CAC'GTC
Cuts at: 1318 2395 1318
Size: 1077 4528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2125 3336
Size: 416 53 83 399 24 682 26 1211
Cuts at: 3336 4294 442
Size: 958 1753
Fragments arranged by size:
1753 1211 958 682 416 399 83 53
26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2687 4265 1239
Size: 1133 315 1578 2579
Fragments arranged by size:
2579 1578 1133 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 2519 4268 882
Size: 617 990 30 1749 2219
Fragments arranged by size:
2219 1749 990 617 30
BsaAI yAC'GTr
Cuts at: 493 1204 2902 3396 493
Size: 711 1698 494 2702
Fragments arranged by size:
2702 1698 711 494
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2360 3412 3794 4429
Size: 53 83 186 936 827 1052 382 635
Cuts at: 4429 275
Size: 1451
Fragments arranged by size:
1451 1052 936 827 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5605
BseYI C'CCAG_C
Cuts at: 2412 2728 4808 5304 2412
Size: 316 2080 496 2713
Fragments arranged by size:
2713 2080 496 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 2887 1163
Size: 573 705 446 3881
Fragments arranged by size:
3881 705 573 446
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 3666 3751 4822 2181
Size: 1485 85 1071 2964
Fragments arranged by size:
2964 1485 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
BsmBI CGTCTCn'nnnn_
Cuts at: 2598 2598
Size: 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2357 2924 3666 3751 4822 1391
Size: 399 391 176 567 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 391 176 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2375 2465 2960 844
Size: 1531 90 495 3489
Fragments arranged by size:
3489 1531 495 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 4249 4436 1700
Size: 110 230 2209 187 2869
Fragments arranged by size:
2869 2209 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 2616 1925
Size: 691 4914
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2433 2637 794
Size: 1639 204 3762
Fragments arranged by size:
3762 1639 204
BstBI TT'CG_AA
Cuts at: 2007 4409 2007
Size: 2402 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600
Size: 963 367 385 17 1452 86 12 346
Cuts at: 5600 1972
Size: 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 3121 3915 3935 2429
Size: 692 794 20 4099
Fragments arranged by size:
4099 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2824 2859 529
Size: 35 2260 35 3275
Fragments arranged by size:
3275 2260 35 35
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 2832 3052
Size: 54 1425 50 275 18 649 353 220
Cuts at: 3052 3941 5570 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 649 353 275 220 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2687 3668 4265 5052 5320 1239
Size: 393 740 315 981 597 787 268 1524
Fragments arranged by size:
1524 981 787 740 597 393 315 268
EcoO109I rG'GnC_Cy
Cuts at: 2053 2711 2053
Size: 658 4947
EcoRI G'AATT_C
Cuts at: 2444 2914 3292 2444
Size: 470 378 4757
Fragments arranged by size:
4757 470 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2575 4752 1678
Size: 897 2177 2531
Fragments arranged by size:
2531 2177 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2743 2775 4246 4278 4320
Size: 32 135 32 1171 32 1471 32 42
Cuts at: 4320 4352 1373
Size: 32 2626
Fragments arranged by size:
2626 1471 1171 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2041 2930 2041
Size: 889 4716
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2324 4719 4903 1305
Size: 1019 2395 184 2007
Fragments arranged by size:
2395 2007 1019 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 2834 10
Size: 54 1425 343 649 353 2781
Fragments arranged by size:
2781 1425 649 353 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2282 2469 3021 3698 4846 5091 1606
Size: 465 211 187 552 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 465 245 211 187
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NsiI A_TGCA'T
Cuts at: 2144 2144
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 4318
PfoI T'CCnGG_A
Cuts at: 2388 2388
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2711 2053
Size: 658 4947
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2406 2957 830
Size: 1576 551 3478
Fragments arranged by size:
3478 1576 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2282 2469 2282
Size: 187 5418
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SanDI GG'GwC_CC
Cuts at: 2711 2711
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2249 2402 2953 4088 4769 4960 826
Size: 219 1204 153 551 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 551 219 191 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2521 2741 2932 1872
Size: 649 220 191 4545
Fragments arranged by size:
4545 649 220 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2902 3396 493
Size: 2409 494 2702
Fragments arranged by size:
2702 2409 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 3044 513
Size: 552 1979 3074
Fragments arranged by size:
3074 1979 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2150 2845 3851
Size: 274 80 33 51 1443 173 695 1006
Cuts at: 3851 5563 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1006 695 274 173 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2519 2739 2930 1870
Size: 649 220 191 4545
Fragments arranged by size:
4545 649 220 191
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI