Restriction map for
pFN21ASDA0157
(Circular) (Six-base) MAPSORT of: pFN21ASDA0157.seq Check: 11 from: 1 to: 5974
pFN21ASDA0157 5974 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 2132 4101 4474 1563
Size: 569 1969 373 3063
Fragments arranged by size:
3063 1969 569 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2035 2395 2449 2535 2654 2913 3089 4037
Size: 403 360 54 86 119 259 176 948
Cuts at: 4037 5421 5689 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 948 403 360 268 259 176
119 86 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2168 3157 3283 3509 3661 5770 1493
Size: 675 989 126 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 675 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3056 3089 3056
Size: 33 5941
BamHI G'GATC_C
Cuts at: 2929 3304 3671 2929
Size: 375 367 5232
Fragments arranged by size:
5232 375 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3038 3293 1391
Size: 1647 255 4072
Fragments arranged by size:
4072 1647 255
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 2764 928
Size: 971 642 223 4138
Fragments arranged by size:
4138 971 642 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2907 2941 4165 4199 1903
Size: 34 970 34 1224 34 3678
Fragments arranged by size:
3678 1224 970 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3866 5076 3084
Size: 782 1210 3982
Fragments arranged by size:
3982 1210 782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2391 4035 5191 1790
Size: 601 1644 1156 2573
Fragments arranged by size:
2573 1644 1156 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 3705 4663
Size: 416 53 83 399 24 700 1588 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1588 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3006 3006
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3138 4634 1239
Size: 1899 1496 2579
Fragments arranged by size:
2579 1899 1496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3009 4099 4964 5262 3009
Size: 1090 865 298 3721
Fragments arranged by size:
3721 1090 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3271 3765 493
Size: 711 2067 494 2702
Fragments arranged by size:
2702 2067 711 494
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 3781 4163 4798
Size: 53 83 186 936 497 1751 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1751 1451 936 635 497 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2925 3090 1186
Size: 1081 658 165 4070
Fragments arranged by size:
4070 1081 658 165
BseYI C'CCAG_C
Cuts at: 2851 5177 5673 2851
Size: 2326 496 3152
Fragments arranged by size:
3152 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2715 1163
Size: 573 979 4422
Fragments arranged by size:
4422 979 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2463 4035 4120 5191 2463
Size: 1572 85 1071 3246
Fragments arranged by size:
3246 1572 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2463 3038 3293 4035 4120 5191
Size: 399 601 72 575 255 742 85 1071
Cuts at: 5191 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 601 575 399 255 85
72
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 3329 844
Size: 1497 988 3489
Fragments arranged by size:
3489 1497 988
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2693 794
Size: 1899 4075
BstBI TT'CG_AA
Cuts at: 2007 4778 2007
Size: 2771 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2929 3304 3671 4056 4073 5525 5611 5623
Size: 957 375 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 957 385 375 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3219 513
Size: 552 957 1197 3268
Fragments arranged by size:
3268 1197 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 2694 2820 3490 4284 4304 2424
Size: 270 126 670 794 20 4094
Fragments arranged by size:
4094 794 670 270 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 2468 3043 3904 1353
Size: 1061 54 575 861 3423
Fragments arranged by size:
3423 1061 861 575 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2395 2449 2535 2654 2913 3089
Size: 393 403 360 54 86 119 259 176
Cuts at: 3089 3138 4037 4634 5421 5689 1239
Size: 49 899 597 787 268 1524
Fragments arranged by size:
1524 899 787 597 403 393 360 268
259 176 119 86 54 49
EcoO109I rG'GnC_Cy
Cuts at: 3238 3238
Size: 5974
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
EcoRV GAT'ATC
Cuts at: 2496 2496
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2076 5121 1678
Size: 398 3045 2531
Fragments arranged by size:
3045 2531 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4615 4647 4689 4721 1373
Size: 32 135 32 3043 32 42 32 2626
Fragments arranged by size:
3043 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2842 748
Size: 2094 3880
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4291 4450 518
Size: 648 2766 359 159 2042
Fragments arranged by size:
2766 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2891 3116 3390 4067 5215 5460 1606
Size: 1285 225 274 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 274 245 225
NcoI C'CATG_G
Cuts at: 513 1065 2022 3219 513
Size: 552 957 1197 3268
Fragments arranged by size:
3268 1197 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PfoI T'CCnGG_A
Cuts at: 3076 3076
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2337 2369 4043 4075 5556 5588 2337
Size: 32 1674 32 1481 32 2723
Fragments arranged by size:
2723 1674 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3238 3238
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2661 3544 2661
Size: 883 5091
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2891 3116 2891
Size: 225 5749
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 2468 3043 2468
Size: 575 5399
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 2977 4222 5934 2977
Size: 1245 1712 3017
Fragments arranged by size:
3017 1712 1245
SfcI C'TryA_G
Cuts at: 826 1045 2341 3171 3322 4457 5138 5329 826
Size: 219 1296 830 151 1135 681 191 1471
Fragments arranged by size:
1471 1296 1135 830 681 219 191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3024 4078 4979 5241 820
Size: 197 926 1081 1054 901 262 1553
Fragments arranged by size:
1553 1081 1054 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3271 3765 493
Size: 2778 494 2702
Fragments arranged by size:
2778 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2924 1517
Size: 1407 4567
StyI C'CwwG_G
Cuts at: 513 1065 2022 3219 3413 513
Size: 552 957 1197 194 3074
Fragments arranged by size:
3074 1197 957 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 2973 4001 4018 4171 4356 4801
Size: 1255 709 684 1028 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1255 1028 709 684 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 2903 2975
Size: 274 80 33 51 1443 561 365 72
Cuts at: 2975 4220 5932 96
Size: 1245 1712 138
Fragments arranged by size:
1712 1443 1245 561 365 274 138 80
72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 2396 1316
Size: 1080 4894
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3113 3113
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI