Restriction map for
pFN21ASDA0153
(Circular) (Six-base) MAPSORT of: pFN21ASDA0153.seq Check: 3696 from: 1 to: 6661
pFN21ASDA0153 6661 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2671 2950 3146 3586 2422
Size: 249 279 196 440 5497
Fragments arranged by size:
5497 440 279 249 196
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3663 3900 4471 278
Size: 53 83 186 936 2127 237 571 2468
Fragments arranged by size:
2468 2127 936 571 237 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2943 3771 3954 4004 1937
Size: 1006 828 183 50 4594
Fragments arranged by size:
4594 1006 828 183 50
Acc65I G'GTAC_C
Cuts at: 3873 3982 3873
Size: 109 6552
AclI AA'CG_TT
Cuts at: 1563 2161 4788 5161 1563
Size: 598 2627 373 3063
Fragments arranged by size:
3063 2627 598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 3803 3886 4724 6108 6376 1632
Size: 1979 192 83 838 1384 268 1917
Fragments arranged by size:
1979 1917 1384 838 268 192 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 5560 6537 1203
Size: 4357 977 1327
Fragments arranged by size:
4357 1327 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 5390 5390
Size: 6661
AleI CACnn'nnGTG
Cuts at: 1166 2951 1166
Size: 1785 4876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2807 3227 1184
Size: 1623 420 4618
Fragments arranged by size:
4618 1623 420
AlwNI CAG_nnn'CTG
Cuts at: 2425 2507 3832 4404 5976 2425
Size: 82 1325 572 1572 3110
Fragments arranged by size:
3110 1572 1325 572 82
ApaI G_GGCC'C
Cuts at: 2803 3173 2803
Size: 370 6291
ApaLI G'TGCA_C
Cuts at: 2915 3599 4718 5874 2915
Size: 684 1119 1156 3702
Fragments arranged by size:
3702 1156 1119 684
ApoI r'AATT_y
Cuts at: 1493 3644 3970 4196 4348 6457 1493
Size: 2151 326 226 152 2109 1697
Fragments arranged by size:
2151 2109 1697 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2071 2271 2299 2438 2739 2837
Size: 73 99 29 200 28 139 301 98
Cuts at: 2837 3164 3620 3753 3986 1870
Size: 327 456 133 233 4545
Fragments arranged by size:
4545 456 327 301 233 200 139 133
99 98 73 29 28
BamHI G'GATC_C
Cuts at: 2622 3991 4358 2622
Size: 1369 367 4925
Fragments arranged by size:
4925 1369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2434 2656 3342 3873 3982
Size: 325 217 627 647 222 686 531 109
Cuts at: 3982 5437 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 686 647 627 531 325 222
217 109
BanII G_rGCy'C
Cuts at: 1391 2349 2803 3173 3980 1391
Size: 958 454 370 807 4072
Fragments arranged by size:
4072 958 807 454 370
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3414 3892 928
Size: 971 1515 478 3697
Fragments arranged by size:
3697 1515 971 478
BbvCI CC'TCA_GC
Cuts at: 2574 2822 2574
Size: 248 6413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4852 4886 1903
Size: 34 2915 34 3678
Fragments arranged by size:
3678 2915 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3168 5272 136
Size: 107 122 71 631 605 1496 2104 1525
Fragments arranged by size:
2104 1525 1496 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3672 6656 3672
Size: 2984 3677
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 2439 2643 2803 2919 3173 3603 4722 5878
Size: 649 204 160 116 254 430 1119 1156
Cuts at: 5878 1790
Size: 2573
Fragments arranged by size:
2573 1156 1119 649 430 254 204 160
116
BmgBI CAC'GTC
Cuts at: 1318 2397 2638 2953 3313 3412 1318
Size: 1079 241 315 360 99 4567
Fragments arranged by size:
4567 1079 360 315 241 99
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 2490 3211
Size: 416 53 83 399 24 759 314 721
Cuts at: 3211 4392 5350 442
Size: 1181 958 1753
Fragments arranged by size:
1753 1181 958 759 721 416 399 314
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2333 2543 2891 3854 5321 1239
Size: 971 123 210 348 963 1467 2579
Fragments arranged by size:
2579 1467 971 963 348 210 123
Bpu10I CC'TnA_GC
Cuts at: 2574 2822 2574
Size: 248 6413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4786 5651 5949 4786
Size: 865 298 5498
Fragments arranged by size:
5498 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5324 882
Size: 617 3825 2219
Fragments arranged by size:
3825 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3958 4452 493
Size: 711 2754 494 2702
Fragments arranged by size:
2754 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 3015 3660 3897
Size: 53 83 186 936 548 934 645 237
Cuts at: 3897 4468 4850 5485 275
Size: 571 382 635 1451
Fragments arranged by size:
1451 936 934 645 635 571 548 382
237 186 83 53
BsaWI w'CCGG_w
Cuts at: 2775 5094 5492 5766 5913 6324 2775
Size: 2319 398 274 147 411 3112
Fragments arranged by size:
3112 2319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 5293
Fragments arranged by size:
5293 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2255 2267 2270 2540 2591 2744 1186
Size: 916 153 12 3 270 51 153 5103
Fragments arranged by size:
5103 916 270 153 153 51 12 3
BseYI C'CCAG_C
Cuts at: 2210 2642 5864 6360 2210
Size: 432 3222 496 2511
Fragments arranged by size:
3222 2511 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2165 2432 2975 3311 1163
Size: 573 372 57 267 543 336 4513
Fragments arranged by size:
4513 573 543 372 336 267 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2412 2856 2919 3356 3603 4722 4807 5878
Size: 216 444 63 437 247 1119 85 1071
Cuts at: 5878 2196
Size: 2979
Fragments arranged by size:
2979 1119 1071 444 437 247 216 85
63
BsmI GAATG_Cn'
Cuts at: 4172 4265 4172
Size: 93 6568
BsmBI CGTCTCn'nnnn_
Cuts at: 2753 2753
Size: 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2349 2412 2439 2643 2803 2856
Size: 399 406 153 63 27 204 160 53
Cuts at: 2856 2919 3173 3356 3603 3980 4722 4807 5878
Size: 63 254 183 247 377 742 85 1071
Cuts at: 5878 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 406 399 377 254 247
204 183 160 153 85 63 63 53
27
BspEI T'CCGG_A
Cuts at: 2775 6324 2775
Size: 3549 3112
BspHI T'CATG_A
Cuts at: 3564 4550 6280 3564
Size: 986 1730 3945
Fragments arranged by size:
3945 1730 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2671 2950 3120 3146 3207 3586 3861
Size: 1578 249 279 170 26 61 379 275
Cuts at: 3861 3953 4016 844
Size: 92 63 3489
Fragments arranged by size:
3489 1578 379 279 275 249 170 92
63 61 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2343 4548 1394
Size: 429 212 308 2205 3507
Fragments arranged by size:
3507 2205 429 308 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 5156 5330 65
Size: 1183 3908 174 1396
Fragments arranged by size:
3908 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 2587 5305 5492 1700
Size: 110 239 538 2718 187 2869
Fragments arranged by size:
2869 2718 538 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6661
BssHII G'CGCG_C
Cuts at: 1925 2094 2140 2414 3146 3293 1925
Size: 169 46 274 732 147 5293
Fragments arranged by size:
5293 732 274 169 147 46
BssSI C'ACGA_G
Cuts at: 2195 4721 5733 2195
Size: 2526 1012 3123
Fragments arranged by size:
3123 2526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 5021
BstBI TT'CG_AA
Cuts at: 2007 5465 2007
Size: 3458 3203
BstEII G'GTnAC_C
Cuts at: 1184 2734 1184
Size: 1550 5111
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 3929 2467
Size: 1462 5199
BstYI r'GATC_y
Cuts at: 1972 2622 3270 3672 3828 3991 4358 4743 4760
Size: 650 648 402 156 163 367 385 17
Cuts at: 4760 6212 6298 6310 6656 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 650 648 402 385 367 346
163 156 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6661
BtgI C'CryG_G
Cuts at: 513 1065 2022 2482 2563 2652 2910 2923 513
Size: 552 957 460 81 89 258 13 4251
Fragments arranged by size:
4251 957 552 460 258 89 81 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3127 487
Size: 683 56 1901 4021
Fragments arranged by size:
4021 1901 683 56
BtsI GCAGTG_nn'
Cuts at: 4177 4971 4991 4177
Size: 794 20 5847
Fragments arranged by size:
5847 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 3965 3965
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3311 5668 809
Size: 507 1995 2357 1802
Fragments arranged by size:
2357 1995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2457 2926
Size: 54 1425 50 275 18 276 351 469
Cuts at: 2926 2964 4108 4997 6626 8
Size: 38 1144 889 1629 43
Fragments arranged by size:
1629 1425 1144 889 469 351 276 275
54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 3032 3603 4591 1353
Size: 891 788 571 988 3423
Fragments arranged by size:
3423 988 891 788 571
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3026 5248 5622 5768 387
Size: 1064 138 1437 2222 374 146 1280
Fragments arranged by size:
2222 1437 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2333 2543 2891 3611 3803 3854
Size: 393 578 123 210 348 720 192 51
Cuts at: 3854 3886 4724 5321 6108 6376 1239
Size: 32 838 597 787 268 1524
Fragments arranged by size:
1524 838 787 720 597 578 393 348
268 210 192 123 51 32
EcoO109I rG'GnC_Cy
Cuts at: 2039 2152 2713 2800 3169 3465 2039
Size: 113 561 87 369 296 5235
Fragments arranged by size:
5235 561 369 296 113 87
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 3203 5167 3203
Size: 1964 4697
HaeII r_GCGC'y
Cuts at: 1678 2050 2123 2138 5808 1678
Size: 372 73 15 3670 2531
Fragments arranged by size:
3670 2531 372 73 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2810 2842 3731 3763 5302
Size: 32 135 32 1238 32 889 32 1539
Cuts at: 5302 5334 5376 5408 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1539 1238 889 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3772 4005 4251 669
Size: 988 281 1834 233 246 3079
Fragments arranged by size:
3079 1834 988 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3877 3986 3877
Size: 109 6552
MfeI C'AATT_G
Cuts at: 4260 4260
Size: 6661
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3534 5775 5959 1305
Size: 2229 2241 184 2007
Fragments arranged by size:
2241 2229 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2966 10
Size: 54 1425 343 627 507 3705
Fragments arranged by size:
3705 1425 627 507 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2810 2951 3563 4619 4978 5137
Size: 648 1068 576 141 612 1056 359 159
Cuts at: 5137 518
Size: 2042
Fragments arranged by size:
2042 1068 1056 648 612 576 359 159
141
MspA1I CmG'CkG
Cuts at: 1606 2425 2557 2573 3241 4077 4754 5902 6147
Size: 819 132 16 668 836 677 1148 245
Cuts at: 6147 1606
Size: 2120
Fragments arranged by size:
2120 1148 836 819 677 668 245 132
16
NaeI GCC'GGC
Cuts at: 2051 2051
Size: 6661
NcoI C'CATG_G
Cuts at: 513 1065 2022 2563 513
Size: 552 957 541 4611
Fragments arranged by size:
4611 957 552 541
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6661
NgoMIV G'CCGG_C
Cuts at: 2049 2049
Size: 6661
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2674 2788 2815 4019 5564 1132
Size: 97 1445 114 27 1204 1545 2229
Fragments arranged by size:
2229 1545 1445 1204 114 97 27
PasI CC'CwG_GG
Cuts at: 1348 2796 3727 1348
Size: 1448 931 4282
Fragments arranged by size:
4282 1448 931
PciI A'CATG_T
Cuts at: 5560 5560
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6661
PfoI T'CCnGG_A
Cuts at: 3831 3831
Size: 6661
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2152 2713 2039
Size: 113 561 5987
Fragments arranged by size:
5987 561 113
PshAI GACnn'nnGTC
Cuts at: 1613 3659 1613
Size: 2046 4615
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PspOMI G'GGCC_C
Cuts at: 2799 3169 2799
Size: 370 6291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 6629
PstI C_TGCA'G
Cuts at: 830 2430 3872 4013 830
Size: 1600 1442 141 3478
Fragments arranged by size:
3478 1600 1442 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
PvuII CAG'CTG
Cuts at: 2425 2557 2573 2425
Size: 132 16 6513
Fragments arranged by size:
6513 132 16
SalI G'TCGA_C
Cuts at: 1936 3770 4003 1936
Size: 1834 233 4594
Fragments arranged by size:
4594 1834 233
SanDI GG'GwC_CC
Cuts at: 2039 2152 2039
Size: 113 6548
SbfI CC_TGCA'GG
Cuts at: 3872 4013 3872
Size: 141 6520
ScaI AGT'ACT
Cuts at: 4909 6621 4909
Size: 1712 4949
SfcI C'TryA_G
Cuts at: 826 1045 2426 3052 3868 4009 5144 5825 6016
Size: 219 1381 626 816 141 1135 681 191
Cuts at: 6016 826
Size: 1471
Fragments arranged by size:
1471 1381 1135 816 681 626 219 191
141
SfiI GGCCn_nnn'nGGCC
Cuts at: 3168 3168
Size: 6661
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 2073 2273 2301 2440 3755 3988 1872
Size: 201 200 28 139 1315 233 4545
Fragments arranged by size:
4545 1315 233 201 200 139 28
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4765 5666 5928 820
Size: 197 926 2822 901 262 1553
Fragments arranged by size:
2822 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3958 4452 493
Size: 3465 494 2702
Fragments arranged by size:
3465 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 2788 4019 1132
Size: 1656 1231 3774
Fragments arranged by size:
3774 1656 1231
SrfI GCCC'GGGC
Cuts at: 2073 2440 2073
Size: 367 6294
SspI AAT'ATT
Cuts at: 5 52 4585 5
Size: 47 4533 2081
Fragments arranged by size:
4533 2081 47
StuI AGG'CCT
Cuts at: 1517 2102 1517
Size: 585 6076
StyI C'CwwG_G
Cuts at: 513 1065 2022 2103 2563 3360 4100 513
Size: 552 957 81 460 797 740 3074
Fragments arranged by size:
3074 957 797 740 552 460 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2993 3014 3323 4688 4705 4858 5043
Size: 1255 1413 21 309 1365 17 153 185
Cuts at: 5043 5488 325
Size: 445 1498
Fragments arranged by size:
1498 1413 1365 1255 445 309 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2498 3375 4907
Size: 274 80 33 51 1443 521 877 1532
Cuts at: 4907 6619 96
Size: 1712 138
Fragments arranged by size:
1712 1532 1443 877 521 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2386 2418 2516 2548 3798 3830 6621 6653 2386
Size: 32 98 32 1250 32 2791 32 2394
Fragments arranged by size:
2791 2394 1250 98 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2561 2812 3648 2561
Size: 251 836 5574
Fragments arranged by size:
5574 836 251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 2071 2271 2299 2438 3753 3986 1870
Size: 201 200 28 139 1315 233 4545
Fragments arranged by size:
4545 1315 233 201 200 139 28
XmnI GAAnn'nnTTC
Cuts at: 3095 3554 4790 3095
Size: 459 1236 4966
Fragments arranged by size:
4966 1236 459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3661 3898 4469 276
Size: 53 83 186 936 2127 237 571 2468
Fragments arranged by size:
2468 2127 936 571 237 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
RsrII SacI SacII SapI SexAI SfoI SwaI