Restriction map for
pFN21ASDA0152
(Circular) (Six-base) MAPSORT of: pFN21ASDA0152.seq Check: 9385 from: 1 to: 5605
pFN21ASDA0152 5605 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 2948 1937
Size: 475 536 4594
Fragments arranged by size:
4594 536 475
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 3668 5052 5320 1632
Size: 1037 999 1384 268 1917
Fragments arranged by size:
1917 1384 1037 999 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2294 1184
Size: 1110 4495
AlwNI CAG_nnn'CTG
Cuts at: 2314 3348 4920 2314
Size: 1034 1572 2999
Fragments arranged by size:
2999 1572 1034
ApaLI G'TGCA_C
Cuts at: 2207 2640 3662 4818 2207
Size: 433 1022 1156 2994
Fragments arranged by size:
2994 1156 1022 433
ApoI r'AATT_y
Cuts at: 1493 2399 2914 3140 3292 5401 1493
Size: 906 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 906 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2156 2930 1870
Size: 73 165 48 774 4545
Fragments arranged by size:
4545 774 165 73 48
BamHI G'GATC_C
Cuts at: 2214 2935 3302 2214
Size: 721 367 4517
Fragments arranged by size:
4517 721 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2115 2924 1391
Size: 724 809 4072
Fragments arranged by size:
4072 809 724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2857 928
Size: 971 958 3676
Fragments arranged by size:
3676 971 958
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 2138 2250
Size: 107 122 71 631 605 457 9 112
Cuts at: 2250 4216 136
Size: 1966 1525
Fragments arranged by size:
1966 1525 631 605 457 122 112 107
71 9
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2364 2644 3666 4822 1790
Size: 421 153 280 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 421 280 153
BmgBI CAC'GTC
Cuts at: 1318 2206 2464 1318
Size: 888 258 4459
Fragments arranged by size:
4459 888 258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4265 1239
Size: 3026 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 4268 882
Size: 617 837 1932 2219
Fragments arranged by size:
2219 1932 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 2386 2902 3396 493
Size: 711 1182 516 494 2702
Fragments arranged by size:
2702 1182 711 516 494
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2387 1186
Size: 1168 33 4404
Fragments arranged by size:
4404 1168 33
BseYI C'CCAG_C
Cuts at: 2026 2427 2657 4808 5304 2026
Size: 401 230 2151 496 2327
Fragments arranged by size:
2327 2151 496 401 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 1163
Size: 573 1068 3964
Fragments arranged by size:
3964 1068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2644 3666 3751 4822 2211
Size: 433 1022 85 1071 2994
Fragments arranged by size:
2994 1071 1022 433 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2211 2364 2644 2924 3666 3751
Size: 399 325 96 153 280 280 742 85
Cuts at: 3751 4822 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 280 280 153
96 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 3492 1394
Size: 429 506 1163 3507
Fragments arranged by size:
3507 1163 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2123 2132 2892 4249 4436 1700
Size: 110 282 31 9 760 1357 187 2869
Fragments arranged by size:
2869 1357 760 282 187 110 31 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 2007 4409 2007
Size: 2402 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2851 2851
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2214 2310 2935 3302 3687 3704 5156 5242
Size: 242 96 625 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 625 385 367 346 242 96
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5605
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2167 2202 529
Size: 35 1603 35 3932
Fragments arranged by size:
3932 1603 35 35
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2467 2467
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2130 2458
Size: 54 1425 50 275 18 291 9 328
Cuts at: 2458 3052 3941 5570 8
Size: 594 889 1629 43
Fragments arranged by size:
1629 1425 889 594 328 291 275 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 2885 3535 1353
Size: 1356 176 650 3423
Fragments arranged by size:
3423 1356 650 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 3668 4265 5052 5320 1239
Size: 393 1037 999 597 787 268 1524
Fragments arranged by size:
1524 1037 999 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2238 2238
Size: 5605
EcoO109I rG'GnC_Cy
Cuts at: 2105 2230 2792 2105
Size: 125 562 4918
Fragments arranged by size:
4918 562 125
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
EcoRV GAT'ATC
Cuts at: 2485 2485
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2063 4752 1678
Size: 385 2689 2531
Fragments arranged by size:
2689 2531 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2302 2334 2732
Size: 32 135 32 525 32 173 32 398
Cuts at: 2732 2764 4246 4278 4320 4352 1373
Size: 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1482 525 398 173 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2179 2949 3195 669
Size: 988 281 241 770 246 3079
Fragments arranged by size:
3079 988 770 281 246 241
HindIII A'AGCT_T
Cuts at: 748 2681 748
Size: 1933 3672
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 2897 4719 4903 1305
Size: 1014 578 1822 184 2007
Fragments arranged by size:
2007 1822 1014 578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2460 10
Size: 54 1425 343 628 3155
Fragments arranged by size:
3155 1425 628 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2849 3563 3922 4081 518
Size: 648 1683 714 359 159 2042
Fragments arranged by size:
2042 1683 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 3021 3698 4846 5091 1606
Size: 448 967 677 1148 245 2120
Fragments arranged by size:
2120 1148 967 677 448 245
NaeI GCC'GGC
Cuts at: 2094 2125 2134 2094
Size: 31 9 5565
Fragments arranged by size:
5565 31 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NgoMIV G'CCGG_C
Cuts at: 2092 2123 2132 2092
Size: 31 9 5565
Fragments arranged by size:
5565 31 9
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2201 2963 4508 1132
Size: 97 972 762 1545 2229
Fragments arranged by size:
2229 1545 972 762 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 1108
Size: 1359 4246
PfoI T'CCnGG_A
Cuts at: 2695 2695
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2105 2230 2105
Size: 125 5480
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2957 830
Size: 2127 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SanDI GG'GwC_CC
Cuts at: 2105 2105
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2953 4088 4769 4960 826
Size: 219 1908 1135 681 191 1471
Fragments arranged by size:
1908 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2129 2250 2129
Size: 121 5484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 2092 1700
Size: 392 5213
SmaI CCC'GGG
Cuts at: 1872 2110 2932 1872
Size: 238 822 4545
Fragments arranged by size:
4545 822 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2902 3396 493
Size: 2409 494 2702
Fragments arranged by size:
2702 2409 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2631 3044 513
Size: 552 1566 413 3074
Fragments arranged by size:
3074 1566 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 3632 3649 3802 3987 4432 325
Size: 1255 1238 814 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1238 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 2542 2574 5565 5597 2040
Size: 32 470 32 2991 32 2048
Fragments arranged by size:
2991 2048 470 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2108 2930 1870
Size: 238 822 4545
Fragments arranged by size:
4545 822 238
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfoI SrfI SwaI XcmI