Restriction map for
pFN21ASDA0141
(Circular) (Six-base) MAPSORT of: pFN21ASDA0141.seq Check: 2964 from: 1 to: 6274
pFN21ASDA0141 6274 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3186 3595 3186
Size: 409 5865
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 3386 4337 5721 5989 1632
Size: 1436 318 951 1384 268 1917
Fragments arranged by size:
1917 1436 1384 951 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 5173 6150 1203
Size: 3970 977 1327
Fragments arranged by size:
3970 1327 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2883 1184
Size: 1699 4575
AlwNI CAG_nnn'CTG
Cuts at: 4017 5589 4017
Size: 1572 4702
ApaI G_GGCC'C
Cuts at: 3342 3342
Size: 6274
ApaLI G'TGCA_C
Cuts at: 4331 5487 4331
Size: 1156 5118
ApoI r'AATT_y
Cuts at: 1493 3198 3583 3809 3961 6070 1493
Size: 1705 385 226 152 2109 1697
Fragments arranged by size:
2109 1705 1697 385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3092 3334 3439 3599 1870
Size: 73 92 1057 242 105 160 4545
Fragments arranged by size:
4545 1057 242 160 105 92 73
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2421 2897 2985 3186
Size: 325 217 627 241 393 476 88 201
Cuts at: 3186 3595 5050 618
Size: 409 1455 1842
Fragments arranged by size:
1842 1455 627 476 409 393 325 241
217 201 88
BanII G_rGCy'C
Cuts at: 1391 2056 2569 3117 3310 3342 3593 1391
Size: 665 513 548 193 32 251 4072
Fragments arranged by size:
4072 665 548 513 251 193 32
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 6274
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 928
Size: 971 336 4967
Fragments arranged by size:
4967 971 336
BbvCI CC'TCA_GC
Cuts at: 2358 2524 2661 2358
Size: 166 137 5971
Fragments arranged by size:
5971 166 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 2466 4885
Size: 107 122 71 631 605 631 163 2419
Cuts at: 4885 136
Size: 1525
Fragments arranged by size:
2419 1525 631 631 605 163 122 107
71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 2529 3691 2529
Size: 1162 5112
Bme1580I G_kGCm'C
Cuts at: 1790 2291 3342 4335 5491 1790
Size: 501 1051 993 1156 2573
Fragments arranged by size:
2573 1156 1051 993 501
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 4005 4963
Size: 416 53 83 399 24 661 1927 958
Cuts at: 4963 442
Size: 1753
Fragments arranged by size:
1927 1753 958 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3413 1056
Size: 2357 3917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2109 2583 2109
Size: 474 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2104 3361 3487 4934 1239
Size: 865 1257 126 1447 2579
Fragments arranged by size:
2579 1447 1257 865 126
Bpu10I CC'TnA_GC
Cuts at: 2358 2524 2661 3021 2358
Size: 166 137 360 5611
Fragments arranged by size:
5611 360 166 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3091 3268 4399 5264 5562 2729
Size: 362 177 1131 865 298 3441
Fragments arranged by size:
3441 1131 865 362 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2991 4937 882
Size: 617 1492 1946 2219
Fragments arranged by size:
2219 1946 1492 617
BsaAI yAC'GTr
Cuts at: 493 1204 3571 4065 493
Size: 711 2367 494 2702
Fragments arranged by size:
2702 2367 711 494
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 4081 4463 5098
Size: 53 83 186 936 496 2052 382 635
Cuts at: 5098 275
Size: 1451
Fragments arranged by size:
2052 1451 936 635 496 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2436 2444 2466 1178
Size: 30 165 30 1011 22 8 22 4986
Fragments arranged by size:
4986 1011 165 30 30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2122 2422 2577 2726 3452 1186
Size: 936 300 155 149 726 4008
Fragments arranged by size:
4008 936 726 300 155 149
BseYI C'CCAG_C
Cuts at: 2456 2470 2655 3001 3341 5477 5973 2456
Size: 14 185 346 340 2136 496 2757
Fragments arranged by size:
2757 2136 496 346 340 185 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3245 1163
Size: 573 1509 4192
Fragments arranged by size:
4192 1509 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2056 2569 3446 4335 4420 5491 2056
Size: 513 877 889 85 1071 2839
Fragments arranged by size:
2839 1071 889 877 513 85
BsmI GAATG_Cn'
Cuts at: 3785 3878 3785
Size: 93 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2291 2569 3117 3310 3342 3446
Size: 399 266 235 278 548 193 32 104
Cuts at: 3446 3593 4335 4420 5491 1391
Size: 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 548 399 278 266 235
193 147 104 85 32
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 2881 4163 5893 2881
Size: 1282 1730 3262
Fragments arranged by size:
3262 1730 1282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3174 3629 844
Size: 2330 455 3489
Fragments arranged by size:
3489 2330 455
BsrBI CCG'CTC
Cuts at: 1394 1823 2196 3025 3272 4161 1394
Size: 429 373 829 247 889 3507
Fragments arranged by size:
3507 889 829 429 373 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 4769 4943 65
Size: 1183 3521 174 1396
Fragments arranged by size:
3521 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4918 5105 1700
Size: 110 3108 187 2869
Fragments arranged by size:
3108 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 2191 4334 5346 2191
Size: 2143 1012 3119
Fragments arranged by size:
3119 2143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 2007 5078 2007
Size: 3071 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2143
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2325 3604 3971 4356 4373 5825 5911 5923
Size: 353 1279 367 385 17 1452 86 12
Cuts at: 5923 6269 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1279 385 367 353 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2492 2574 2492
Size: 82 6192
BtgI C'CryG_G
Cuts at: 513 1065 2179 2212 2842 3318 513
Size: 552 1114 33 630 476 3469
Fragments arranged by size:
3469 1114 630 552 476 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2743 487
Size: 683 56 1517 4018
Fragments arranged by size:
4018 1517 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 2435 3042 3790 4584 4604 2371
Size: 64 607 748 794 20 4041
Fragments arranged by size:
4041 794 748 607 64 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2098 5281 809
Size: 507 782 3183 1802
Fragments arranged by size:
3183 1802 782 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2509 3721
Size: 54 1425 50 275 18 465 214 1212
Cuts at: 3721 4610 6239 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1212 889 465 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2744 2786 3013 4204 1353
Size: 708 683 42 227 1191 3423
Fragments arranged by size:
3423 1191 708 683 227 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4861 5235 5381 387
Size: 1064 138 3272 374 146 1280
Fragments arranged by size:
3272 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2054 2567 2054
Size: 513 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 3068 3361 3386 3487 4337 4934
Size: 393 472 964 293 25 101 850 597
Cuts at: 4934 5721 5989 1239
Size: 787 268 1524
Fragments arranged by size:
1524 964 850 787 597 472 393 293
268 101 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 3505 2711
Size: 794 5480
EcoO109I rG'GnC_Cy
Cuts at: 2072 2174 2208 2354 2578 2594 3338 2072
Size: 102 34 146 224 16 744 5008
Fragments arranged by size:
5008 744 224 146 102 34 16
EcoRI G'AATT_C
Cuts at: 3583 3961 3583
Size: 378 5896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 6274
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 2032 5421 1678
Size: 354 3389 2531
Fragments arranged by size:
3389 2531 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2185 2217 2264
Size: 32 135 32 492 32 89 32 47
Cuts at: 2264 2296 2433 2465 2990 3022 4915 4947 4989
Size: 32 137 32 525 32 1893 32 42
Cuts at: 4989 5021 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 525 492 137 135 89 47
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3618 3864 669
Size: 988 281 1680 246 3079
Fragments arranged by size:
3079 1680 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3084 748
Size: 2336 3938
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 6274
KpnI G_GTAC'C
Cuts at: 3190 3599 3190
Size: 409 5865
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5388 5572 1305
Size: 4083 184 2007
Fragments arranged by size:
4083 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2511 10
Size: 54 1425 343 679 3773
Fragments arranged by size:
3773 1425 679 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2886 4232 4591 4750 518
Size: 648 1720 1346 359 159 2042
Fragments arranged by size:
2042 1720 1346 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2314 2362 2844 3690 4367 5515 5760 1606
Size: 708 48 482 846 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 708 677 482 245 48
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 6274
NcoI C'CATG_G
Cuts at: 513 1065 2179 2212 3318 513
Size: 552 1114 33 1106 3469
Fragments arranged by size:
3469 1114 1106 552 33
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6274
NheI G'CTAG_C
Cuts at: 1052 3409 1052
Size: 2357 3917
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2621 3437 3632 5177 1132
Size: 97 1392 816 195 1545 2229
Fragments arranged by size:
2229 1545 1392 816 195 97
PasI CC'CwG_GG
Cuts at: 1348 2904 1348
Size: 1556 4718
PciI A'CATG_T
Cuts at: 5173 5173
Size: 6274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 2962 1108
Size: 1175 679 4420
Fragments arranged by size:
4420 1175 679
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2174 2208 2354 2578 2594 2072
Size: 102 34 146 224 16 5752
Fragments arranged by size:
5752 224 146 102 34 16
PshAI GACnn'nnGTC
Cuts at: 1613 3367 1613
Size: 1754 4520
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PspOMI G'GGCC_C
Cuts at: 3338 3338
Size: 6274
PspXI vC'TCGA_Gb
Cuts at: 3439 3439
Size: 6274
PstI C_TGCA'G
Cuts at: 830 2313 2839 3061 3331 3395 3470 3626 830
Size: 1483 526 222 270 64 75 156 3478
Fragments arranged by size:
3478 1483 526 270 222 156 75 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 2314 2314
Size: 6274
SacI G_AGCT'C
Cuts at: 2056 2569 2056
Size: 513 5761
SacII CC_GC'GG
Cuts at: 2845 2845
Size: 6274
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SapI GCTCTTCn'nnn_
Cuts at: 2061 2744 2061
Size: 683 5591
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 4522 6234 4522
Size: 1712 4562
SfcI C'TryA_G
Cuts at: 826 1045 2309 2835 2848 3057 3327 3391 3466
Size: 219 1264 526 13 209 270 64 75
Cuts at: 3466 3622 4757 5438 5629 826
Size: 156 1135 681 191 1471
Fragments arranged by size:
1471 1264 1135 681 526 270 219 209
191 156 75 64 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 6274
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 6274
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 2037 3336 3601 1872
Size: 165 1299 265 4545
Fragments arranged by size:
4545 1299 265 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 3070 3247 3439 4378 5279
Size: 197 926 765 362 177 192 939 901
Cuts at: 5279 5541 820
Size: 262 1553
Fragments arranged by size:
1553 939 926 901 765 362 262 197
192 177
SnaBI TAC'GTA
Cuts at: 493 3571 4065 493
Size: 3078 494 2702
Fragments arranged by size:
3078 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 2621 3632 1132
Size: 1489 1011 3774
Fragments arranged by size:
3774 1489 1011
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 3216 3245 1517
Size: 1699 29 4546
Fragments arranged by size:
4546 1699 29
StyI C'CwwG_G
Cuts at: 513 1065 2179 2204 2212 3108 3193 3217 3318
Size: 552 1114 25 8 896 85 24 101
Cuts at: 3318 3454 3713 513
Size: 136 259 3074
Fragments arranged by size:
3074 1114 896 552 259 136 101 85
25 24 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2439 4301 4318 4471 4656 5101 325
Size: 1255 859 1862 17 153 185 445 1498
Fragments arranged by size:
1862 1498 1255 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2862 4520 6232
Size: 274 80 33 51 1443 885 1658 1712
Cuts at: 6232 96
Size: 138
Fragments arranged by size:
1712 1658 1443 885 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 6234 6266 2414
Size: 32 3788 32 2422
Fragments arranged by size:
3788 2422 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6274
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3009 3009
Size: 6274
XhoI C'TCGA_G
Cuts at: 1943 3439 1943
Size: 1496 4778
XmaI C'CCGG_G
Cuts at: 1870 2035 3334 3599 1870
Size: 165 1299 265 4545
Fragments arranged by size:
4545 1299 265 165
XmnI GAAnn'nnTTC
Cuts at: 3371 4403 3371
Size: 1032 5242
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI
SexAI SrfI SwaI