Restriction map for
pFN21ASDA0137
(Circular) (Six-base) MAPSORT of: pFN21ASDA0137.seq Check: 5116 from: 1 to: 7027
pFN21ASDA0137 7027 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4837 278
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4370 1937
Size: 2433 4594
Acc65I G'GTAC_C
Cuts at: 4348 4348
Size: 7027
AclI AA'CG_TT
Cuts at: 1563 3289 5154 5527 1563
Size: 1726 1865 373 3063
Fragments arranged by size:
3063 1865 1726 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3692 4236 5090 6474 6742 1632
Size: 2060 544 854 1384 268 1917
Fragments arranged by size:
2060 1917 1384 854 544 268
AflII C'TTAA_G
Cuts at: 820 1017 3782 820
Size: 197 2765 4065
Fragments arranged by size:
4065 2765 197
AflIII A'CryG_T
Cuts at: 1203 5926 6903 1203
Size: 4723 977 1327
Fragments arranged by size:
4723 1327 977
AgeI A'CCGG_T
Cuts at: 4123 5858 4123
Size: 1735 5292
AhdI GACnn_n'nnGTC
Cuts at: 5756 5756
Size: 7027
AleI CACnn'nnGTG
Cuts at: 1166 3318 1166
Size: 2152 4875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7027
AlwNI CAG_nnn'CTG
Cuts at: 2716 4770 6342 2716
Size: 2054 1572 3401
Fragments arranged by size:
3401 2054 1572
ApaLI G'TGCA_C
Cuts at: 4196 5084 6240 4196
Size: 888 1156 4983
Fragments arranged by size:
4983 1156 888
ApoI r'AATT_y
Cuts at: 1493 2539 3178 3225 4246 4336 4562 4714 6823
Size: 1046 639 47 1021 90 226 152 2109
Cuts at: 6823 1493
Size: 1697
Fragments arranged by size:
2109 1697 1046 1021 639 226 152 90
47
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2921 4352 1870
Size: 73 152 826 1431 4545
Fragments arranged by size:
4545 1431 826 152 73
BamHI G'GATC_C
Cuts at: 2188 4357 4724 2188
Size: 2169 367 4491
Fragments arranged by size:
4491 2169 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4348 5803 618
Size: 325 217 627 1310 1251 1455 1842
Fragments arranged by size:
1842 1455 1310 1251 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 4346 1391
Size: 711 2244 4072
Fragments arranged by size:
4072 2244 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5218 5252 1903
Size: 34 3281 34 3678
Fragments arranged by size:
3678 3281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3604 4919 6129 3604
Size: 1315 1210 4502
Fragments arranged by size:
4502 1315 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7027
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5638 136
Size: 107 122 71 631 605 3966 1525
Fragments arranged by size:
3966 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7022 7022
Size: 7027
BlpI GC'TnA_GC
Cuts at: 4444 4444
Size: 7027
Bme1580I G_kGCm'C
Cuts at: 1790 2928 4200 5088 6244 1790
Size: 1138 1272 888 1156 2573
Fragments arranged by size:
2573 1272 1156 1138 888
BmgBI CAC'GTC
Cuts at: 1318 2085 2447 1318
Size: 767 362 5898
Fragments arranged by size:
5898 767 362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4758 5716 442
Size: 416 53 83 399 24 3341 958 1753
Fragments arranged by size:
3341 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 3520 5687 1239
Size: 1008 1273 2167 2579
Fragments arranged by size:
2579 2167 1273 1008
Bpu10I CC'TnA_GC
Cuts at: 2750 2750
Size: 7027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2479 5152 6017 6315 2479
Size: 2673 865 298 3191
Fragments arranged by size:
3191 2673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5690 882
Size: 617 4191 2219
Fragments arranged by size:
4191 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3320 4324 4818 493
Size: 711 2116 1004 494 2702
Fragments arranged by size:
2702 2116 1004 711 494
BsaBI GATnn'nnATC
Cuts at: 3666 7021 3666
Size: 3355 3672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4834 5216 5851 275
Size: 53 83 186 936 3301 382 635 1451
Fragments arranged by size:
3301 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2356 4123 5460 5858 6132 6279 6690 2356
Size: 1767 1337 398 274 147 411 2693
Fragments arranged by size:
2693 1767 1337 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6802
Fragments arranged by size:
6802 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2523 1186
Size: 1337 5690
BseYI C'CCAG_C
Cuts at: 2073 4271 6230 6726 2073
Size: 2198 1959 496 2374
Fragments arranged by size:
2374 2198 1959 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2579 4477 5238 6266 1540
Size: 275 186 578 1898 761 1028 2301
Fragments arranged by size:
2301 1898 1028 761 578 275 186
BsiHKAI G_wGCw'C
Cuts at: 2246 4200 5088 5173 6244 2246
Size: 1954 888 85 1071 3029
Fragments arranged by size:
3029 1954 1071 888 85
BsmI GAATG_Cn'
Cuts at: 4538 4631 4538
Size: 93 6934
BsmBI CGTCTCn'nnnn_
Cuts at: 2091 2091
Size: 7027
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2246 2928 4200 4346 5088 5173
Size: 399 312 144 682 1272 146 742 85
Cuts at: 5173 6244 1391
Size: 1071 2174
Fragments arranged by size:
2174 1272 1071 742 682 399 312 146
144 85
BspEI T'CCGG_A
Cuts at: 6690 6690
Size: 7027
BspHI T'CATG_A
Cuts at: 3211 4916 6646 3211
Size: 1705 1730 3592
Fragments arranged by size:
3592 1730 1705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4382 844
Size: 3538 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4914 1394
Size: 429 3091 3507
Fragments arranged by size:
3507 3091 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2172 3658 5522 5696 65
Size: 1183 924 1486 1864 174 1396
Fragments arranged by size:
1864 1486 1396 1183 924 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 5671 5858 1700
Size: 110 2313 1548 187 2869
Fragments arranged by size:
2869 2313 1548 187 110
BsrGI T'GTAC_A
Cuts at: 96 2626 3626 96
Size: 2530 1000 3497
Fragments arranged by size:
3497 2530 1000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 5087 6099 5087
Size: 1012 6015
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7027
BstBI TT'CG_AA
Cuts at: 2007 5831 2007
Size: 3824 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstXI CCAn_nnnn'nTGG
Cuts at: 3862 3862
Size: 7027
BstYI r'GATC_y
Cuts at: 1972 2188 2327 3895 4357 4724 5109 5126 6578
Size: 216 139 1568 462 367 385 17 1452
Cuts at: 6578 6664 6676 7022 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1568 1452 462 385 367 346 216
139 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6288
Fragments arranged by size:
6288 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 2272 4141 4543 5337 5357 2047
Size: 225 1869 402 794 20 3717
Fragments arranged by size:
3717 1869 794 402 225 20
ClaI AT'CG_AT
Cuts at: 1554 2911 4186 1554
Size: 1357 1275 4395
Fragments arranged by size:
4395 1357 1275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3174 3209 529
Size: 35 2610 35 4347
Fragments arranged by size:
4347 2610 35 35
DraI TTT'AAA
Cuts at: 2650 4331 2650
Size: 1681 5346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6034 809
Size: 507 4718 1802
Fragments arranged by size:
4718 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2449 4474
Size: 54 1425 50 275 18 282 337 2025
Cuts at: 4474 5363 6992 8
Size: 889 1629 43
Fragments arranged by size:
2025 1629 1425 889 337 282 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4474 1537
Size: 275 2662 4090
Fragments arranged by size:
4090 2662 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2891 3255 4957 1353
Size: 1538 364 1702 3423
Fragments arranged by size:
3423 1702 1538 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5614 5988 6134 387
Size: 1064 138 4025 374 146 1280
Fragments arranged by size:
4025 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 3520 3692 4236 5090 5687 6474
Size: 393 615 1273 172 544 854 597 787
Cuts at: 6474 6742 1239
Size: 268 1524
Fragments arranged by size:
1524 1273 854 787 615 597 544 393
268 172
EcoO109I rG'GnC_Cy
Cuts at: 2118 2713 2118
Size: 595 6432
EcoRI G'AATT_C
Cuts at: 4336 4714 4336
Size: 378 6649
EcoRV GAT'ATC
Cuts at: 3743 3743
Size: 7027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4088 4088
Size: 7027
FspI TGC'GCA
Cuts at: 5533 5533
Size: 7027
HaeII r_GCGC'y
Cuts at: 1678 2952 6174 1678
Size: 1274 3222 2531
Fragments arranged by size:
3222 2531 1274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 2904 2936 3587
Size: 32 135 32 758 32 542 32 651
Cuts at: 3587 3619 3980 4012 5668 5700 5742 5774 1373
Size: 32 361 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 758 651 542 361 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 4371 4617 669
Size: 988 281 1257 1176 246 3079
Fragments arranged by size:
3079 1257 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2320 748
Size: 1572 5455
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KpnI G_GTAC'C
Cuts at: 4352 4352
Size: 7027
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 6903 6903
Size: 7027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2114 2251 3934 3982 4002 6141 6325 1305
Size: 809 137 1683 48 20 2139 184 2007
Fragments arranged by size:
2139 2007 1683 809 184 137 48 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2451 10
Size: 54 1425 343 619 4586
Fragments arranged by size:
4586 1425 619 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3318 3531 4985 5344 5503 518
Size: 648 1671 481 213 1454 359 159 2042
Fragments arranged by size:
2042 1671 1454 648 481 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 4204 4443 5120 6268 6513 1606
Size: 2598 239 677 1148 245 2120
Fragments arranged by size:
2598 2120 1148 677 245 239
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6475
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7027
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7027
NotI GC'GGCC_GC
Cuts at: 4474 4474
Size: 7027
NspI r_CATG'y
Cuts at: 1132 1229 3536 3822 4385 5930 1132
Size: 97 2307 286 563 1545 2229
Fragments arranged by size:
2307 2229 1545 563 286 97
PasI CC'CwG_GG
Cuts at: 1348 2154 3907 1348
Size: 806 1753 4468
Fragments arranged by size:
4468 1753 806
PciI A'CATG_T
Cuts at: 5926 5926
Size: 7027
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 1108
Size: 1608 5419
PfoI T'CCnGG_A
Cuts at: 2144 2709 2144
Size: 565 6462
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 6609 6641 5096
Size: 32 1481 32 5482
Fragments arranged by size:
5482 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2713 2118
Size: 595 6432
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7027
PsiI TTA'TAA
Cuts at: 4154 4597 4154
Size: 443 6584
PstI C_TGCA'G
Cuts at: 830 3542 4379 830
Size: 2712 837 3478
Fragments arranged by size:
3478 2712 837
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 4204 4204
Size: 7027
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 5275 6987 5275
Size: 1712 5315
SfcI C'TryA_G
Cuts at: 826 1045 3538 4375 5510 6191 6382 826
Size: 219 2493 837 1135 681 191 1471
Fragments arranged by size:
2493 1471 1135 837 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 2097 4354 1872
Size: 225 2257 4545
Fragments arranged by size:
4545 2257 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2494 3782 5131 6032 6294 820
Size: 197 926 551 1288 1349 901 262 1553
Fragments arranged by size:
1553 1349 1288 926 901 551 262 197
SnaBI TAC'GTA
Cuts at: 493 4324 4818 493
Size: 3831 494 2702
Fragments arranged by size:
3831 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7027
SphI G_CATG'C
Cuts at: 1132 4385 1132
Size: 3253 3774
SspI AAT'ATT
Cuts at: 5 52 4089 4951 5
Size: 47 4037 862 2081
Fragments arranged by size:
4037 2081 862 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7027
StyI C'CwwG_G
Cuts at: 513 1065 3606 4143 4466 513
Size: 552 2541 537 323 3074
Fragments arranged by size:
3074 2541 552 537 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2593 3004 3384 3696 5054 5071 5224
Size: 1255 1013 411 380 312 1358 17 153
Cuts at: 5224 5409 5854 325
Size: 185 445 1498
Fragments arranged by size:
1498 1358 1255 1013 445 411 380 312
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2626 2867 3626
Size: 274 80 33 51 1443 649 241 759
Cuts at: 3626 5273 6985 96
Size: 1647 1712 138
Fragments arranged by size:
1712 1647 1443 759 649 274 241 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6987 7019 6987
Size: 32 6995
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7027
XbaI T'CTAG_A
Cuts at: 4363 4363
Size: 7027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 2486 2039
Size: 447 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7027
XmaI C'CCGG_G
Cuts at: 1870 2095 4352 1870
Size: 225 2257 4545
Fragments arranged by size:
4545 2257 225
XmnI GAAnn'nnTTC
Cuts at: 5156 5156
Size: 7027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4835 276
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI