Restriction map for
pFN21ASDA0126
(Circular) (Six-base) MAPSORT of: pFN21ASDA0126.seq Check: 552 from: 1 to: 7948
pFN21ASDA0126 7948 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3796 3796
Size: 7948
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5758 278
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5291 1937
Size: 3354 4594
Acc65I G'GTAC_C
Cuts at: 4607 5269 4607
Size: 662 7286
AclI AA'CG_TT
Cuts at: 1563 6075 6448 1563
Size: 4512 373 3063
Fragments arranged by size:
4512 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 3218 3392 4729 6011 7395 7663 1632
Size: 518 1068 174 1337 1282 1384 268 1917
Fragments arranged by size:
1917 1384 1337 1282 1068 518 268 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7751
AflIII A'CryG_T
Cuts at: 1203 4162 4305 6847 7824 1203
Size: 2959 143 2542 977 1327
Fragments arranged by size:
2959 2542 1327 977 143
AgeI A'CCGG_T
Cuts at: 6779 6779
Size: 7948
AhdI GACnn_n'nnGTC
Cuts at: 6677 6677
Size: 7948
AleI CACnn'nnGTG
Cuts at: 1166 5044 1166
Size: 3878 4070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2641 3288 1184
Size: 1457 647 5844
Fragments arranged by size:
5844 1457 647
AlwNI CAG_nnn'CTG
Cuts at: 3196 5691 7263 3196
Size: 2495 1572 3881
Fragments arranged by size:
3881 2495 1572
ApaLI G'TGCA_C
Cuts at: 6005 7161 6005
Size: 1156 6792
ApoI r'AATT_y
Cuts at: 1493 2201 3920 4232 4366 4751 4822 5257 5483
Size: 708 1719 312 134 385 71 435 226
Cuts at: 5483 5635 7744 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1719 1697 708 435 385 312 226
152 134 71
AseI AT'TA_AT
Cuts at: 160 1032 6502 160
Size: 872 5470 1606
Fragments arranged by size:
5470 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
AvaI C'yCGr_G
Cuts at: 1870 1943 5273 1870
Size: 73 3330 4545
Fragments arranged by size:
4545 3330 73
BamHI G'GATC_C
Cuts at: 5023 5278 5645 5023
Size: 255 367 7326
Fragments arranged by size:
7326 367 255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4607 4724 5269 6724 618
Size: 325 217 627 2820 117 545 1455 1842
Fragments arranged by size:
2820 1842 1455 627 545 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2743 2898 3857 5267 1391
Size: 1352 155 959 1410 4072
Fragments arranged by size:
4072 1410 1352 959 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3029 928
Size: 971 1130 5847
Fragments arranged by size:
5847 1130 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6139 6173 1903
Size: 34 4202 34 3678
Fragments arranged by size:
4202 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4150 4299 5840 7050 4150
Size: 149 1541 1210 5048
Fragments arranged by size:
5048 1541 1210 149
BclI T'GATC_A
Cuts at: 1548 3622 1548
Size: 2074 5874
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 7948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6559 136
Size: 107 122 71 631 605 4887 1525
Fragments arranged by size:
4887 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2676 2760 2887 3183 7943 2676
Size: 84 127 296 4760 2681
Fragments arranged by size:
4760 2681 296 127 84
BlpI GC'TnA_GC
Cuts at: 3681 4665 5365 3681
Size: 984 700 6264
Fragments arranged by size:
6264 984 700
Bme1580I G_kGCm'C
Cuts at: 1790 3442 4842 6009 7165 1790
Size: 1652 1400 1167 1156 2573
Fragments arranged by size:
2573 1652 1400 1167 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5679 6637 442
Size: 416 53 83 399 24 4262 958 1753
Fragments arranged by size:
4262 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2160 1056
Size: 1104 6844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3194 3204 4025 4503 6608 1239
Size: 1955 10 821 478 2105 2579
Fragments arranged by size:
2579 2105 1955 821 478 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 3428 6073 6938 7236 2038
Size: 1390 2645 865 298 2750
Fragments arranged by size:
2750 2645 1390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4236 6611 882
Size: 617 2737 2375 2219
Fragments arranged by size:
2737 2375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5245 5739 493
Size: 711 4041 494 2702
Fragments arranged by size:
4041 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3740 4323 7942 3740
Size: 583 3619 3746
Fragments arranged by size:
3746 3619 583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5755 6137 6772 275
Size: 53 83 186 936 4222 382 635 1451
Fragments arranged by size:
4222 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4155 6381 6779 7053 7200 7611 4155
Size: 2226 398 274 147 411 4492
Fragments arranged by size:
4492 2226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 3524 3554 4380
Size: 30 165 30 1137 30 954 30 826
Cuts at: 4380 4410 1178
Size: 30 4716
Fragments arranged by size:
4716 1137 954 826 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3404 4832 1186
Size: 2218 1428 4302
Fragments arranged by size:
4302 2218 1428
BseYI C'CCAG_C
Cuts at: 2152 2324 3031 3520 4767 7151 7647 2152
Size: 172 707 489 1247 2384 496 2453
Fragments arranged by size:
2453 2384 1247 707 496 489 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 2647 3548 4774 1163
Size: 573 906 5 901 1226 4337
Fragments arranged by size:
4337 1226 906 901 573 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5398 6159 7187 1540
Size: 275 186 3397 761 1028 2301
Fragments arranged by size:
3397 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2898 5039 6009 6094 7165 2898
Size: 2141 970 85 1071 3681
Fragments arranged by size:
3681 2141 1071 970 85
BsmI GAATG_Cn'
Cuts at: 5459 5552 5459
Size: 93 7855
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2743 2898 3442 3857 4842 5039 5267
Size: 399 953 155 544 415 985 197 228
Cuts at: 5267 6009 6094 7165 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 985 953 742 544 415 399
228 197 155 85
BspEI T'CCGG_A
Cuts at: 7611 7611
Size: 7948
BspHI T'CATG_A
Cuts at: 2847 5837 7567 2847
Size: 2990 1730 3228
Fragments arranged by size:
3228 2990 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2613 3652 3796 4419 5303 844
Size: 1769 1039 144 623 884 3489
Fragments arranged by size:
3489 1769 1039 884 623 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2232 5835 1394
Size: 429 409 3603 3507
Fragments arranged by size:
3603 3507 429 409
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 3853 5097 6443 6617 65
Size: 1183 1177 1428 1244 1346 174 1396
Fragments arranged by size:
1428 1396 1346 1244 1183 1177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6592 6779 1700
Size: 110 4782 187 2869
Fragments arranged by size:
4782 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3481 96
Size: 3385 4563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7948
BssSI C'ACGA_G
Cuts at: 6008 7020 6008
Size: 1012 6936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3658 794
Size: 2864 5084
BstBI TT'CG_AA
Cuts at: 2007 6752 2007
Size: 4745 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7948
BstYI r'GATC_y
Cuts at: 1972 2292 2458 2676 2760 2887 2904 3183 3401
Size: 320 166 218 84 127 17 279 218
Cuts at: 3401 5023 5090 5128 5163 5278 5645 6030 6047
Size: 1622 67 38 35 115 367 385 17
Cuts at: 6047 7499 7585 7597 7943 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1622 1452 385 367 346 320 279
218 218 166 127 115 86 84 67
38 35 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 4070 4070
Size: 7948
BtgI C'CryG_G
Cuts at: 513 1065 3813 5154 513
Size: 552 2748 1341 3307
Fragments arranged by size:
3307 2748 1341 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7209
Fragments arranged by size:
7209 683 56
BtsI GCAGTG_nn'
Cuts at: 2625 2630 4107 5464 6258 6278 2625
Size: 5 1477 1357 794 20 4295
Fragments arranged by size:
4295 1477 1357 794 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7913
DraI TTT'AAA
Cuts at: 5252 5252
Size: 7948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6955 809
Size: 507 5639 1802
Fragments arranged by size:
5639 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3837 5168 5395
Size: 54 1425 50 275 18 2007 1331 227
Cuts at: 5395 6284 7913 8
Size: 889 1629 43
Fragments arranged by size:
2007 1629 1425 1331 889 275 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5395 1537
Size: 275 3583 4090
Fragments arranged by size:
4090 3583 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 2574 2787 4980 5878 1353
Size: 1088 133 213 2193 898 3423
Fragments arranged by size:
3423 2193 1088 898 213 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3950 6535 6909 7055 387
Size: 1064 138 2361 2585 374 146 1280
Fragments arranged by size:
2585 2361 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2896 2896
Size: 7948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 3194 3204 3218 3392 4025 4503
Size: 393 518 1044 10 14 174 633 478
Cuts at: 4503 4729 6011 6608 7395 7663 1239
Size: 226 1282 597 787 268 1524
Fragments arranged by size:
1524 1282 1044 787 633 597 518 478
393 268 226 174 14 10
EcoO109I rG'GnC_Cy
Cuts at: 2431 2995 2431
Size: 564 7384
EcoRI G'AATT_C
Cuts at: 2201 5257 5635 2201
Size: 3056 378 4514
Fragments arranged by size:
4514 3056 378
EcoRV GAT'ATC
Cuts at: 4786 4786
Size: 7948
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 4660 2423
Size: 2237 5711
FspI TGC'GCA
Cuts at: 6454 6454
Size: 7948
HaeII r_GCGC'y
Cuts at: 1678 7095 1678
Size: 5417 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3730 3762 4114 4146 4343
Size: 32 135 32 2158 32 352 32 197
Cuts at: 4343 4375 4622 4654 5097 5129 6589 6621 6663
Size: 32 247 32 443 32 1460 32 42
Cuts at: 6663 6695 1373
Size: 32 2626
Fragments arranged by size:
2626 2158 1460 443 352 247 197 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 5292 5538 669
Size: 988 281 748 2606 246 3079
Fragments arranged by size:
3079 2606 988 748 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7948
HpaI GTT'AAC
Cuts at: 5538 5538
Size: 7948
KpnI G_GTAC'C
Cuts at: 4611 5273 4611
Size: 662 7286
MfeI C'AATT_G
Cuts at: 5547 5547
Size: 7948
MluI A'CGCG_T
Cuts at: 7824 7824
Size: 7948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4074 4999 5221 7062 7246 1305
Size: 2769 925 222 1841 184 2007
Fragments arranged by size:
2769 2007 1841 925 222 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3839 10
Size: 54 1425 343 2007 4119
Fragments arranged by size:
4119 2007 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3270 4679 5042 5044 5906 6265 6424
Size: 648 2104 1409 363 2 862 359 159
Cuts at: 6424 518
Size: 2042
Fragments arranged by size:
2104 2042 1409 862 648 363 359 159
2
MspA1I CmG'CkG
Cuts at: 1606 2128 3524 4567 5364 6041 7189 7434 1606
Size: 522 1396 1043 797 677 1148 245 2120
Fragments arranged by size:
2120 1396 1148 1043 797 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 3813 5154 513
Size: 552 2748 1341 3307
Fragments arranged by size:
3307 2748 1341 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7948
NheI G'CTAG_C
Cuts at: 1052 2156 1052
Size: 1104 6844
NotI GC'GGCC_GC
Cuts at: 5395 5395
Size: 7948
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 7948
NspI r_CATG'y
Cuts at: 1132 1229 3269 4013 4309 5306 6851 1132
Size: 97 2040 744 296 997 1545 2229
Fragments arranged by size:
2229 2040 1545 997 744 296 97
PacI TTA_AT'TAA
Cuts at: 2478 2478
Size: 7948
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7948
PciI A'CATG_T
Cuts at: 4305 6847 4305
Size: 2542 5406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3291 1108
Size: 2183 5765
PfoI T'CCnGG_A
Cuts at: 5019 5019
Size: 7948
PmeI GTTT'AAAC
Cuts at: 5252 5252
Size: 7948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6017 6049 7530 7562 6017
Size: 32 1481 32 6403
Fragments arranged by size:
6403 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2995 2995
Size: 7948
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7948
PsiI TTA'TAA
Cuts at: 5518 5518
Size: 7948
PstI C_TGCA'G
Cuts at: 830 3663 5300 830
Size: 2833 1637 3478
Fragments arranged by size:
3478 2833 1637
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7948
PvuII CAG'CTG
Cuts at: 2128 3524 4567 2128
Size: 1396 1043 5509
Fragments arranged by size:
5509 1396 1043
SacI G_AGCT'C
Cuts at: 2898 2898
Size: 7948
SalI G'TCGA_C
Cuts at: 1936 5290 1936
Size: 3354 4594
SbfI CC_TGCA'GG
Cuts at: 5300 5300
Size: 7948
ScaI AGT'ACT
Cuts at: 6196 7908 6196
Size: 1712 6236
SexAI A'CCwGG_T
Cuts at: 4704 4704
Size: 7948
SfcI C'TryA_G
Cuts at: 826 1045 3659 3895 4550 5296 6431 7112 7303
Size: 219 2614 236 655 746 1135 681 191
Cuts at: 7303 826
Size: 1471
Fragments arranged by size:
2614 1471 1135 746 681 655 236 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7948
SmaI CCC'GGG
Cuts at: 1872 5275 1872
Size: 3403 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3443 6052 6953 7215 820
Size: 197 926 110 1390 2609 901 262 1553
Fragments arranged by size:
2609 1553 1390 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 5245 5739 493
Size: 4752 494 2702
Fragments arranged by size:
4752 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7948
SphI G_CATG'C
Cuts at: 1132 3269 5306 1132
Size: 2137 2037 3774
Fragments arranged by size:
3774 2137 2037
SspI AAT'ATT
Cuts at: 5 52 5872 5
Size: 47 5820 2081
Fragments arranged by size:
5820 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7948
StyI C'CwwG_G
Cuts at: 513 1065 2574 2991 3813 4134 4842 5154 5387
Size: 552 1509 417 822 321 708 312 233
Cuts at: 5387 513
Size: 3074
Fragments arranged by size:
3074 1509 822 708 552 417 321 312
233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3347 3929 4712 5975 5992 6145 6330
Size: 1255 1767 582 783 1263 17 153 185
Cuts at: 6330 6775 325
Size: 445 1498
Fragments arranged by size:
1767 1498 1263 1255 783 582 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 3136 3481
Size: 274 80 33 51 1443 771 388 345
Cuts at: 3481 3588 3597 6194 7906 96
Size: 107 9 2597 1712 138
Fragments arranged by size:
2597 1712 1443 771 388 345 274 138
107 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3409 3441 4380 4412 7908 7940 3409
Size: 32 939 32 3496 32 3417
Fragments arranged by size:
3496 3417 939 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7948
XbaI T'CTAG_A
Cuts at: 2890 5284 2890
Size: 2394 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4435 5080 4435
Size: 645 7303
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7948
XmaI C'CCGG_G
Cuts at: 1870 5273 1870
Size: 3403 4545
XmnI GAAnn'nnTTC
Cuts at: 2982 4691 6077 2982
Size: 1709 1386 4853
Fragments arranged by size:
4853 1709 1386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5756 276
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI