Restriction map for
pFN21ASDA0123
(Circular) (Six-base) MAPSORT of: pFN21ASDA0123.seq Check: 5700 from: 1 to: 6304
pFN21ASDA0123 6304 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3367 3367
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2378 3647 1937
Size: 441 1269 4594
Fragments arranged by size:
4594 1269 441
Acc65I G'GTAC_C
Cuts at: 3625 3625
Size: 6304
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4367 5751 6019 1632
Size: 2735 1384 268 1917
Fragments arranged by size:
2735 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 2931 5203 6180 1203
Size: 1728 2272 977 1327
Fragments arranged by size:
2272 1728 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 6272
AlwNI CAG_nnn'CTG
Cuts at: 3382 4047 5619 3382
Size: 665 1572 4067
Fragments arranged by size:
4067 1572 665
ApaI G_GGCC'C
Cuts at: 2954 3087 2954
Size: 133 6171
ApaLI G'TGCA_C
Cuts at: 3418 4361 5517 3418
Size: 943 1156 4205
Fragments arranged by size:
4205 1156 943
ApoI r'AATT_y
Cuts at: 1493 2295 2406 3613 3839 3991 6100 1493
Size: 802 111 1207 226 152 2109 1697
Fragments arranged by size:
2109 1697 1207 802 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2966 3629 1870
Size: 73 122 901 663 4545
Fragments arranged by size:
4545 901 663 122 73
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 3132 3567 3625 5080
Size: 325 217 627 345 1000 435 58 1455
Cuts at: 5080 618
Size: 1842
Fragments arranged by size:
1842 1455 1000 627 435 345 325 217
58
BanII G_rGCy'C
Cuts at: 1391 2066 2954 2973 3087 3623 1391
Size: 675 888 19 114 536 4072
Fragments arranged by size:
4072 888 675 536 114 19
BbeI G_GCGC'C
Cuts at: 3571 3571
Size: 6304
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5333
BbvCI CC'TCA_GC
Cuts at: 2090 2090
Size: 6304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 4495 4529 1903
Size: 34 98 34 2426 34 3678
Fragments arranged by size:
3678 2426 98 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 4915 136
Size: 107 122 71 631 605 850 2393 1525
Fragments arranged by size:
2393 1525 850 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2885 6299 2885
Size: 3414 2890
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 2824 2954 3087 3422 4365 5521 1790
Size: 1034 130 133 335 943 1156 2573
Fragments arranged by size:
2573 1156 1034 943 335 133 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 2913 4035
Size: 416 53 83 399 24 1473 23 1122
Cuts at: 4035 4993 442
Size: 958 1753
Fragments arranged by size:
1753 1473 1122 958 416 399 83 53
24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 2618 3014 3032 3088 3379 4964 1239
Size: 951 428 396 18 56 291 1585 2579
Fragments arranged by size:
2579 1585 951 428 396 291 56 18
Bpu10I CC'TnA_GC
Cuts at: 2090 2090
Size: 6304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4429 5294 5592 4429
Size: 865 298 5141
Fragments arranged by size:
5141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4967 882
Size: 617 3468 2219
Fragments arranged by size:
3468 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 3601 4095 493
Size: 711 2324 73 494 2702
Fragments arranged by size:
2702 2324 711 494 73
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 4111 4493 5128
Size: 53 83 186 936 2035 543 382 635
Cuts at: 5128 275
Size: 1451
Fragments arranged by size:
2035 1451 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3594 4737 5135 5409 5556 5967 3594
Size: 1143 398 274 147 411 3931
Fragments arranged by size:
3931 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3140 3170 1178
Size: 30 165 30 1737 30 4312
Fragments arranged by size:
4312 1737 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3029 1186
Size: 1843 4461
BseYI C'CCAG_C
Cuts at: 5507 6003 5507
Size: 496 5808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5731
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2770 3754 4515 5543 1540
Size: 275 186 769 984 761 1028 2301
Fragments arranged by size:
2301 1028 984 769 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 2973 3126 3422 3537 4365 4450 5521 2802
Size: 171 153 296 115 828 85 1071 3585
Fragments arranged by size:
3585 1071 828 296 171 153 115 85
BsmI GAATG_Cn'
Cuts at: 2737 3815 3908 2737
Size: 1078 93 5133
Fragments arranged by size:
5133 1078 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2802 2824 2954 2973 3087 3126
Size: 399 276 736 22 130 19 114 39
Cuts at: 3126 3422 3537 3623 4365 4450 5521 1391
Size: 296 115 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 399 296 276 130
115 114 86 85 39 22 19
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 2650 3327 4193 5923 2650
Size: 677 866 1730 3031
Fragments arranged by size:
3031 1730 866 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2619 3367 3528 3659 844
Size: 1775 748 161 131 3489
Fragments arranged by size:
3489 1775 748 161 131
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2783 2848 3487 3585 4948 5135 1700
Size: 110 973 65 639 98 1363 187 2869
Fragments arranged by size:
2869 1363 973 639 187 110 98 65
BsrGI T'GTAC_A
Cuts at: 96 2285 96
Size: 2189 4115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 2801 3410 4364 5376 2801
Size: 609 954 1012 3729
Fragments arranged by size:
3729 1012 954 609
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 2007 5108 2007
Size: 3101 3203
BstEII G'GTnAC_C
Cuts at: 1184 3506 1184
Size: 2322 3982
BstXI CCAn_nnnn'nTGG
Cuts at: 2903 2903
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2885 3634 4001 4386 4403 5855 5941 5953
Size: 913 749 367 385 17 1452 86 12
Cuts at: 5953 6299 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 913 749 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2747 2747
Size: 6304
BtgI C'CryG_G
Cuts at: 513 1065 2022 2871 3276 513
Size: 552 957 849 405 3541
Fragments arranged by size:
3541 957 849 552 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 3042 3498 3820 4614 4634 3042
Size: 456 322 794 20 4712
Fragments arranged by size:
4712 794 456 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3550 5311 809
Size: 507 2234 1761 1802
Fragments arranged by size:
2234 1802 1761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 3384 3495
Size: 54 1425 50 275 18 951 603 111
Cuts at: 3495 3751 4640 6269 8
Size: 256 889 1629 43
Fragments arranged by size:
1629 1425 951 889 603 275 256 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2161 3449 3596 4234 1353
Size: 808 1288 147 638 3423
Fragments arranged by size:
3423 1288 808 638 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3072 3285 4891 5265 5411 387
Size: 1064 138 1483 213 1606 374 146 1280
Fragments arranged by size:
1606 1483 1280 1064 374 213 146 138
EcoICRI GAG'CTC
Cuts at: 2971 2971
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2618 3014 3032 3088 3379 4367
Size: 393 558 428 396 18 56 291 988
Cuts at: 4367 4964 5751 6019 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 988 787 597 558 428 396 393
291 268 56 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2746 3014 2746
Size: 268 6036
EcoO109I rG'GnC_Cy
Cuts at: 2602 2602
Size: 6304
EcoRI G'AATT_C
Cuts at: 2295 3613 3991 2295
Size: 1318 378 4608
Fragments arranged by size:
4608 1318 378
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 3210 3571 5451 1678
Size: 1532 361 1880 2531
Fragments arranged by size:
2531 1880 1532 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4945 4977 5019 5051 1373
Size: 32 135 32 3373 32 42 32 2626
Fragments arranged by size:
3373 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KasI G'GCGC_C
Cuts at: 3567 3567
Size: 6304
KpnI G_GTAC'C
Cuts at: 3629 3629
Size: 6304
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 2968 5418 5602 1305
Size: 1095 568 2450 184 2007
Fragments arranged by size:
2450 2007 1095 568 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3386 10
Size: 54 1425 343 1554 2928
Fragments arranged by size:
2928 1554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 2642 3050 4262 4621 4780 518
Size: 648 1302 174 408 1212 359 159 2042
Fragments arranged by size:
2042 1302 1212 648 408 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 3313 3720 4397 5545 5790 1606
Size: 1707 407 677 1148 245 2120
Fragments arranged by size:
2120 1707 1148 677 407 245
NaeI GCC'GGC
Cuts at: 2785 2850 3489 2785
Size: 65 639 5600
Fragments arranged by size:
5600 639 65
NarI GG'CG_CC
Cuts at: 3568 3568
Size: 6304
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4795
Fragments arranged by size:
4795 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NgoMIV G'CCGG_C
Cuts at: 2783 2848 3487 2783
Size: 65 639 5600
Fragments arranged by size:
5600 639 65
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 2935 3100 3662 5207 1132
Size: 97 1706 165 562 1545 2229
Fragments arranged by size:
2229 1706 1545 562 165 97
PasI CC'CwG_GG
Cuts at: 1348 3502 1348
Size: 2154 4150
PciI A'CATG_T
Cuts at: 2931 5203 2931
Size: 2272 4032
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6304
PfoI T'CCnGG_A
Cuts at: 3009 3009
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 2602
Size: 6304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2950 3083 2950
Size: 133 6171
PstI C_TGCA'G
Cuts at: 830 2541 3656 830
Size: 1711 1115 3478
Fragments arranged by size:
3478 1711 1115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 3313 3313
Size: 6304
SacI G_AGCT'C
Cuts at: 2973 2973
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SapI GCTCTTCn'nnn_
Cuts at: 3596 3596
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2193 2537 3652 4787 5468 5659 826
Size: 219 1148 344 1115 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 1115 681 344 219 191
SfoI GGC'GCC
Cuts at: 3569 3569
Size: 6304
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4408 5309 5571 820
Size: 197 926 2465 901 262 1553
Fragments arranged by size:
2465 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3601 4095 493
Size: 3108 494 2702
Fragments arranged by size:
3108 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 3349 1517
Size: 1832 4472
StyI C'CwwG_G
Cuts at: 513 1065 2022 3743 513
Size: 552 957 1721 3074
Fragments arranged by size:
3074 1721 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 4331 4348 4501 4686 5131 325
Size: 1255 1914 837 17 153 185 445 1498
Fragments arranged by size:
1914 1498 1255 837 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2285 2898 4550
Size: 274 80 33 51 1443 308 613 1652
Cuts at: 4550 6262 96
Size: 1712 138
Fragments arranged by size:
1712 1652 1443 613 308 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2944 2029
Size: 915 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI