Restriction map for
pFN21ASDA0122
(Circular) (Six-base) MAPSORT of: pFN21ASDA0122.seq Check: 4642 from: 1 to: 7516
pFN21ASDA0122 7516 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3726 5326 278
Size: 53 83 186 936 2190 1600 2468
Fragments arranged by size:
2468 2190 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3318 3599 4557 4859 1937
Size: 1381 281 958 302 4594
Fragments arranged by size:
4594 1381 958 302 281
Acc65I G'GTAC_C
Cuts at: 3287 4837 3287
Size: 1550 5966
AclI AA'CG_TT
Cuts at: 1563 5643 6016 1563
Size: 4080 373 3063
Fragments arranged by size:
4080 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2505 5579 6963 7231 1632
Size: 873 3074 1384 268 1917
Fragments arranged by size:
3074 1917 1384 873 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7319
AflIII A'CryG_T
Cuts at: 1203 6415 7392 1203
Size: 5212 977 1327
Fragments arranged by size:
5212 1327 977
AgeI A'CCGG_T
Cuts at: 6347 6347
Size: 7516
AhdI GACnn_n'nnGTC
Cuts at: 3490 6245 3490
Size: 2755 4761
AleI CACnn'nnGTG
Cuts at: 1166 2640 1166
Size: 1474 6042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4628 1184
Size: 3444 4072
AlwNI CAG_nnn'CTG
Cuts at: 2444 2761 2794 2827 3097 3106 3115 3835 4085
Size: 317 33 33 270 9 9 720 250
Cuts at: 4085 4094 4294 4553 5259 6831 2444
Size: 9 200 259 706 1572 3129
Fragments arranged by size:
3129 1572 720 706 317 270 259 250
200 33 33 9 9 9
ApaI G_GGCC'C
Cuts at: 2980 3416 3997 4259 2980
Size: 436 581 262 6237
Fragments arranged by size:
6237 581 436 262
ApaLI G'TGCA_C
Cuts at: 4719 5573 6729 4719
Size: 854 1156 5506
Fragments arranged by size:
5506 1156 854
ApoI r'AATT_y
Cuts at: 1493 4825 5051 5203 7312 1493
Size: 3332 226 152 2109 1697
Fragments arranged by size:
3332 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6070 160
Size: 872 5038 1606
Fragments arranged by size:
5038 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 2242 2313 3458 4109 4502 4575 4841
Size: 73 299 71 1145 651 393 73 266
Cuts at: 4841 1870
Size: 4545
Fragments arranged by size:
4545 1145 651 393 299 266 73 73
71
BamHI G'GATC_C
Cuts at: 3439 4846 5213 3439
Size: 1407 367 5742
Fragments arranged by size:
5742 1407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 3287 3404 3910 4347
Size: 325 217 627 1196 304 117 506 437
Cuts at: 4347 4837 6292 618
Size: 490 1455 1842
Fragments arranged by size:
1842 1455 1196 627 506 490 437 325
304 217 117
BanII G_rGCy'C
Cuts at: 1391 2249 2894 2980 3416 3636 3644 3997 4259
Size: 858 645 86 436 220 8 353 262
Cuts at: 4259 4368 4402 4518 4709 4835 1391
Size: 109 34 116 191 126 4072
Fragments arranged by size:
4072 858 645 436 353 262 220 191
126 116 109 86 34 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3366 928
Size: 971 1467 5078
Fragments arranged by size:
5078 1467 971
BbvCI CC'TCA_GC
Cuts at: 2420 2420
Size: 7516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2700 2734 4556 4590 5707 5741 1903
Size: 34 763 34 1822 34 1117 34 3678
Fragments arranged by size:
3678 1822 1117 763 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5408 6618 5408
Size: 1210 6306
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 2295 2304
Size: 107 122 71 631 605 506 117 9
Cuts at: 2304 3224 3254 6127 136
Size: 920 30 2873 1525
Fragments arranged by size:
2873 1525 920 631 605 506 122 117
107 71 30 9
BglII A'GATC_T
Cuts at: 7511 7511
Size: 7516
BlpI GC'TnA_GC
Cuts at: 2816 4933 2816
Size: 2117 5399
Bme1580I G_kGCm'C
Cuts at: 1790 2739 2980 3368 3407 3416 3578 3913 3997
Size: 949 241 388 39 9 162 335 84
Cuts at: 3997 4259 4723 5577 6733 1790
Size: 262 464 854 1156 2573
Fragments arranged by size:
2573 1156 949 854 464 388 335 262
241 162 84 39 9
BmgBI CAC'GTC
Cuts at: 1318 2039 1318
Size: 721 6795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2810 3244 3796
Size: 416 53 83 399 24 1393 434 552
Cuts at: 3796 3850 4801 5247 6205 442
Size: 54 951 446 958 1753
Fragments arranged by size:
1753 1393 958 951 552 446 434 416
399 83 54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7516
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3468 3620 3896 6176 1239
Size: 2229 152 276 2280 2579
Fragments arranged by size:
2579 2280 2229 276 152
Bpu10I CC'TnA_GC
Cuts at: 2420 4260 2420
Size: 1840 5676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 4403 5641 6506 6804 3383
Size: 1020 1238 865 298 4095
Fragments arranged by size:
4095 1238 1020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 3740 6179 882
Size: 617 613 1628 2439 2219
Fragments arranged by size:
2439 2219 1628 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 4813 5307 493
Size: 711 3609 494 2702
Fragments arranged by size:
3609 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7510 7510
Size: 7516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 3723 4315 5323
Size: 53 83 186 936 1158 1032 592 1008
Cuts at: 5323 5705 6340 275
Size: 382 635 1451
Fragments arranged by size:
1451 1158 1032 1008 936 635 592 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5949 6347 6621 6768 7179 5949
Size: 398 274 147 411 6286
Fragments arranged by size:
6286 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 3173 3203 3782
Size: 30 165 30 819 30 921 30 579
Cuts at: 3782 3812 1178
Size: 30 4882
Fragments arranged by size:
4882 921 819 579 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2375 2378 2381 2384 2387 2390 2399
Size: 1036 153 3 3 3 3 3 9
Cuts at: 2399 2402 2405 2408 2995 3320 4250 4253 4283
Size: 3 3 3 587 325 930 3 30
Cuts at: 4283 4537 1186
Size: 254 4165
Fragments arranged by size:
4165 1036 930 587 325 254 153 30
9 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 3090 3516 3990 4325 4585 6719 7215 3090
Size: 426 474 335 260 2134 496 3391
Fragments arranged by size:
3391 2134 496 474 426 335 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3101 4491 4757 1163
Size: 573 1365 1390 266 3922
Fragments arranged by size:
3922 1390 1365 573 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3318 3888 4180 4966 5727 6755
Size: 275 186 1317 570 292 786 761 1028
Cuts at: 6755 1540
Size: 2301
Fragments arranged by size:
2301 1317 1028 786 761 570 292 275
186
BsiHKAI G_wGCw'C
Cuts at: 3624 3636 4126 4723 5577 5662 6733 3624
Size: 12 490 597 854 85 1071 4407
Fragments arranged by size:
4407 1071 854 597 490 85 12
BsmI GAATG_Cn'
Cuts at: 5027 5120 5027
Size: 93 7423
BsmBI CGTCTCn'nnnn_
Cuts at: 2310 3730 2310
Size: 1420 6096
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2739 2894 2980 3368 3407 3416
Size: 399 459 490 155 86 388 39 9
Cuts at: 3416 3578 3624 3636 3644 3913 3997 4126 4259
Size: 162 46 12 8 269 84 129 133
Cuts at: 4259 4368 4402 4518 4709 4723 4835 5577 5662
Size: 109 34 116 191 14 112 742 85
Cuts at: 5662 6733 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 490 459 399 388 269
191 162 155 133 129 116 112 109
86 85 84 46 39 34 14 12
9 8
BspEI T'CCGG_A
Cuts at: 7179 7179
Size: 7516
BspHI T'CATG_A
Cuts at: 5405 7135 5405
Size: 1730 5786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 4871 844
Size: 2599 1428 3489
Fragments arranged by size:
3489 2599 1428
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 4264 5403 1394
Size: 429 262 2179 1139 3507
Fragments arranged by size:
3507 2179 1139 429 262
BsrDI GCAATG_nn'
Cuts at: 65 1248 3237 6011 6185 65
Size: 1183 1989 2774 174 1396
Fragments arranged by size:
2774 1989 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 3313 3670 3776 4392 6160 6347
Size: 110 484 1019 357 106 616 1768 187
Cuts at: 6347 1700
Size: 2869
Fragments arranged by size:
2869 1768 1019 616 484 357 187 110
106
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7516
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7516
BssSI C'ACGA_G
Cuts at: 2773 5576 6588 2773
Size: 2803 1012 3701
Fragments arranged by size:
3701 2803 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2482 2761 2794 3106 3115 794
Size: 1688 279 33 312 9 5195
Fragments arranged by size:
5195 1688 312 279 33 9
BstBI TT'CG_AA
Cuts at: 2007 6320 2007
Size: 4313 3203
BstEII G'GTnAC_C
Cuts at: 1184 4741 4786 1184
Size: 3557 45 3914
Fragments arranged by size:
3914 3557 45
BstXI CCAn_nnnn'nTGG
Cuts at: 3982 3982
Size: 7516
BstYI r'GATC_y
Cuts at: 1972 3102 3439 4153 4846 5213 5598 5615 7067
Size: 1130 337 714 693 367 385 17 1452
Cuts at: 7067 7153 7165 7511 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1130 714 693 385 367 346
337 86 17 12
BstZ17I GTA'TAC
Cuts at: 3600 3600
Size: 7516
BtgI C'CryG_G
Cuts at: 513 1065 2022 2458 2958 3049 3388 4623 513
Size: 552 957 436 500 91 339 1235 3406
Fragments arranged by size:
3406 1235 957 552 500 436 339 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6777
Fragments arranged by size:
6777 683 56
BtsI GCAGTG_nn'
Cuts at: 2792 3231 3576 3684 4083 4597 4769 5032 5826
Size: 439 345 108 399 514 172 263 794
Cuts at: 5826 5846 2792
Size: 20 4462
Fragments arranged by size:
4462 794 514 439 399 345 263 172
108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2668 2703 529
Size: 35 2104 35 5342
Fragments arranged by size:
5342 2104 35 35
DraI TTT'AAA
Cuts at: 4820 4820
Size: 7516
DraIII CAC_nnn'GTG
Cuts at: 2482 2804 2482
Size: 322 7194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2043 6523 809
Size: 507 727 4480 1802
Fragments arranged by size:
4480 1802 727 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2461 2806
Size: 54 1425 50 275 18 232 399 345
Cuts at: 2806 3225 3885 4167 4963 5852 7481 8
Size: 419 660 282 796 889 1629 43
Fragments arranged by size:
1629 1425 889 796 660 419 399 345
282 275 232 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3885 4963 1537
Size: 275 2073 1078 4090
Fragments arranged by size:
4090 2073 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5446 1353
Size: 4093 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3871 6103 6477 6623 387
Size: 1064 138 2282 2232 374 146 1280
Fragments arranged by size:
2282 2232 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3634 3634
Size: 7516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2505 3468 3620 3896 5579 6176 6963
Size: 393 873 963 152 276 1683 597 787
Cuts at: 6963 7231 1239
Size: 268 1524
Fragments arranged by size:
1683 1524 963 873 787 597 393 276
268 152
EcoNI CCTnn'n_nnAGG
Cuts at: 2885 4194 2885
Size: 1309 6207
EcoO109I rG'GnC_Cy
Cuts at: 2824 2976 2977 3112 3412 3413 3455 4255 4256
Size: 152 1 135 300 1 42 800 1
Cuts at: 4256 2824
Size: 6084
Fragments arranged by size:
6084 800 300 152 135 42 1 1
1
EcoRI G'AATT_C
Cuts at: 4825 5203 4825
Size: 378 7138
FspI TGC'GCA
Cuts at: 2936 6022 2936
Size: 3086 4430
HaeII r_GCGC'y
Cuts at: 1678 4340 6663 1678
Size: 2662 2323 2531
Fragments arranged by size:
2662 2531 2323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2767 2799 3173
Size: 32 135 32 614 32 549 32 374
Cuts at: 3173 3205 3476 3508 3734 3766 3782 3814 6157
Size: 32 271 32 226 32 16 32 2343
Cuts at: 6157 6189 6231 6263 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2343 614 549 374 271 226 135
42 32 32 32 32 32 32 32
32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3319 4860 5106 669
Size: 988 281 1381 1541 246 3079
Fragments arranged by size:
3079 1541 1381 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7516
HpaI GTT'AAC
Cuts at: 5106 5106
Size: 7516
KpnI G_GTAC'C
Cuts at: 3291 4841 3291
Size: 1550 5966
MfeI C'AATT_G
Cuts at: 5115 5115
Size: 7516
MluI A'CGCG_T
Cuts at: 7392 7392
Size: 7516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3033 4589 6630 6814 1305
Size: 1728 1556 2041 184 2007
Fragments arranged by size:
2041 2007 1728 1556 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2463 3227 4169 10
Size: 54 1425 343 631 764 942 3357
Fragments arranged by size:
3357 1425 942 764 631 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 2414 2640 4148 5474 5833 5992
Size: 648 1024 224 226 1508 1326 359 159
Cuts at: 5992 518
Size: 2042
Fragments arranged by size:
2042 1508 1326 1024 648 359 226 224
159
MspA1I CmG'CkG
Cuts at: 1606 2467 3051 3094 3617 4218 4475 4644 4932
Size: 861 584 43 523 601 257 169 288
Cuts at: 4932 5609 6757 7002 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 861 677 601 584 523 288
257 245 169 43
NcoI C'CATG_G
Cuts at: 513 1065 2022 2958 3388 4623 513
Size: 552 957 936 430 1235 3406
Fragments arranged by size:
3406 1235 957 936 552 430
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7516
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NruI TCG'CGA
Cuts at: 2160 2160
Size: 7516
NspI r_CATG'y
Cuts at: 1132 1229 4874 6419 1132
Size: 97 3645 1545 2229
Fragments arranged by size:
3645 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2857 1348
Size: 1509 6007
PciI A'CATG_T
Cuts at: 6415 6415
Size: 7516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2804 4629 1108
Size: 1696 1825 3995
Fragments arranged by size:
3995 1825 1696
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 7098 7130 5585
Size: 32 1481 32 5971
Fragments arranged by size:
5971 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3455 3455
Size: 7516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7516
PsiI TTA'TAA
Cuts at: 5086 5086
Size: 7516
PspOMI G'GGCC_C
Cuts at: 2976 3412 3993 4255 2976
Size: 436 581 262 6237
Fragments arranged by size:
6237 581 436 262
PstI C_TGCA'G
Cuts at: 830 2472 3102 3111 4090 4620 4868 830
Size: 1642 630 9 979 530 248 3478
Fragments arranged by size:
3478 1642 979 630 530 248 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 2467 3094 4475 2467
Size: 627 1381 5508
Fragments arranged by size:
5508 1381 627
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 7516
SacI G_AGCT'C
Cuts at: 3636 3636
Size: 7516
SacII CC_GC'GG
Cuts at: 3052 3052
Size: 7516
SalI G'TCGA_C
Cuts at: 1936 3317 4858 1936
Size: 1381 1541 4594
Fragments arranged by size:
4594 1541 1381
SbfI CC_TGCA'GG
Cuts at: 3111 4868 3111
Size: 1757 5759
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SfcI C'TryA_G
Cuts at: 826 1045 2468 2638 3098 3107 3322 3700 4086
Size: 219 1423 170 460 9 215 378 386
Cuts at: 4086 4213 4554 4616 4864 5999 6680 6871 826
Size: 127 341 62 248 1135 681 191 1471
Fragments arranged by size:
1471 1423 1135 681 460 386 378 341
248 219 215 191 170 127 62 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3254 3254
Size: 7516
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 4392 1700
Size: 2692 4824
SmaI CCC'GGG
Cuts at: 1872 2244 4843 1872
Size: 372 2599 4545
Fragments arranged by size:
4545 2599 372
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3398 4109 4382 5620 6521 6783
Size: 197 926 1455 711 273 1238 901 262
Cuts at: 6783 820
Size: 1553
Fragments arranged by size:
1553 1455 1238 926 901 711 273 262
197
SnaBI TAC'GTA
Cuts at: 493 4813 5307 493
Size: 4320 494 2702
Fragments arranged by size:
4320 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7516
SphI G_CATG'C
Cuts at: 1132 4874 1132
Size: 3742 3774
SspI AAT'ATT
Cuts at: 5 52 5440 5
Size: 47 5388 2081
Fragments arranged by size:
5388 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 3269 3464 1517
Size: 720 1032 195 5569
Fragments arranged by size:
5569 1032 720 195
StyI C'CwwG_G
Cuts at: 513 1065 2022 2958 3165 3274 3388 3408 3975
Size: 552 957 936 207 109 114 20 567
Cuts at: 3975 4623 4955 513
Size: 648 332 3074
Fragments arranged by size:
3074 957 936 648 567 552 332 207
114 109 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 2792 3135 4706 5543 5560 5713
Size: 1255 783 429 343 1571 837 17 153
Cuts at: 5713 5898 6343 325
Size: 185 445 1498
Fragments arranged by size:
1571 1498 1255 837 783 445 429 343
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3837 5762 7474
Size: 274 80 33 51 1443 1860 1925 1712
Cuts at: 7474 96
Size: 138
Fragments arranged by size:
1925 1860 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 7476 7508 2660
Size: 32 4784 32 2668
Fragments arranged by size:
4784 2668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7516
XbaI T'CTAG_A
Cuts at: 4852 4852
Size: 7516
XhoI C'TCGA_G
Cuts at: 1943 4109 1943
Size: 2166 5350
XmaI C'CCGG_G
Cuts at: 1870 2242 4841 1870
Size: 372 2599 4545
Fragments arranged by size:
4545 2599 372
XmnI GAAnn'nnTTC
Cuts at: 2720 5645 2720
Size: 2925 4591
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3724 5324 276
Size: 53 83 186 936 2190 1600 2468
Fragments arranged by size:
2468 2190 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI
SanDI SapI SexAI SfoI SrfI SwaI XcmI