Restriction map for
pFN21ASDA0120
(Circular) (Six-base) MAPSORT of: pFN21ASDA0120.seq Check: 876 from: 1 to: 5326
pFN21ASDA0120 5326 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3389 4773 5041 1632
Size: 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 268
AfeI AGC'GCT
Cuts at: 2327 2327
Size: 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 2110 2311 3069 4641 2110
Size: 201 758 1572 2795
Fragments arranged by size:
2795 1572 758 201
ApaLI G'TGCA_C
Cuts at: 3383 4539 3383
Size: 1156 4170
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2220 3880 160
Size: 872 1188 1660 1606
Fragments arranged by size:
1660 1606 1188 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2436 2651 1870
Size: 73 291 202 215 4545
Fragments arranged by size:
4545 291 215 202 73
BamHI G'GATC_C
Cuts at: 2461 2656 3023 2461
Size: 195 367 4764
Fragments arranged by size:
4764 367 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2558 2647 4102 618
Size: 325 217 627 771 89 1455 1842
Fragments arranged by size:
1842 1455 771 627 325 217 89
BanII G_rGCy'C
Cuts at: 1391 2217 2451 2645 1391
Size: 826 234 194 4072
Fragments arranged by size:
4072 826 234 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 2298 5321 2095
Size: 203 3023 2100
Fragments arranged by size:
3023 2100 203
BlpI GC'TnA_GC
Cuts at: 2322 2743 2322
Size: 421 4905
Bme1580I G_kGCm'C
Cuts at: 1790 2561 3387 4543 1790
Size: 771 826 1156 2573
Fragments arranged by size:
2573 1156 826 771
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 2243 3057
Size: 416 53 83 399 24 708 118 814
Cuts at: 3057 4015 442
Size: 958 1753
Fragments arranged by size:
1753 958 814 708 416 399 118 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 3986 1239
Size: 881 1866 2579
Fragments arranged by size:
2579 1866 881
Bpu10I CC'TnA_GC
Cuts at: 2051 2051
Size: 5326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3451 4316 4614 2174
Size: 1277 865 298 2886
Fragments arranged by size:
2886 1277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2623 3117 493
Size: 711 1419 494 2702
Fragments arranged by size:
2702 1419 711 494
BsaBI GATnn'nnATC
Cuts at: 2094 2111 5320 2094
Size: 17 3209 2100
Fragments arranged by size:
3209 2100 17
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2573 3133 3515 4150
Size: 53 83 186 936 1040 560 382 635
Cuts at: 4150 275
Size: 1451
Fragments arranged by size:
1451 1040 936 635 560 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3759 4157 4431 4578 4989 3759
Size: 398 274 147 411 4096
Fragments arranged by size:
4096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5101
Fragments arranged by size:
5101 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5326
BseYI C'CCAG_C
Cuts at: 2157 4529 5025 2157
Size: 2372 496 2458
Fragments arranged by size:
2458 2372 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2417 1163
Size: 573 348 333 4072
Fragments arranged by size:
4072 573 348 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2776 3537 4565 1540
Size: 275 186 775 761 1028 2301
Fragments arranged by size:
2301 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3387 3472 4543 2217
Size: 1170 85 1071 3000
Fragments arranged by size:
3000 1170 1071 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
BsmBI CGTCTCn'nnnn_
Cuts at: 2580 2580
Size: 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2451 2561 2645 3387 3472 4543
Size: 399 427 234 110 84 742 85 1071
Cuts at: 4543 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 427 399 234 110 85
84
BspEI T'CCGG_A
Cuts at: 4989 4989
Size: 5326
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2047 2681 844
Size: 1203 634 3489
Fragments arranged by size:
3489 1203 634
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 3970 4157 1700
Size: 110 341 1819 187 2869
Fragments arranged by size:
2869 1819 341 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 2007 4130 2007
Size: 2123 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2095 2106 2259 2298 2461 2613 2656 3023
Size: 123 11 153 39 163 152 43 367
Cuts at: 3023 3408 3425 4877 4963 4975 5321 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 163 153 152
123 86 43 39 17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2022 2274 2566 513
Size: 552 957 252 292 3273
Fragments arranged by size:
3273 957 552 292 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DraIII CAC_nnn'GTG
Cuts at: 2133 2133
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2773 3662
Size: 54 1425 50 275 18 195 748 889
Cuts at: 3662 5291 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 748 275 195 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2773 1537
Size: 275 961 4090
Fragments arranged by size:
4090 961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3256 1353
Size: 1103 800 3423
Fragments arranged by size:
3423 1103 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3389 3986 4773 5041 1239
Size: 393 488 1269 597 787 268 1524
Fragments arranged by size:
1524 1269 787 597 488 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2036 2245 2036
Size: 209 5117
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FseI GG_CCGG'CC
Cuts at: 2155 2155
Size: 5326
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 2329 4473 1678
Size: 651 2144 2531
Fragments arranged by size:
2531 2144 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373
Size: 32 135 32 2395 32 42 32 2626
Fragments arranged by size:
2626 2395 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 10
Size: 54 1425 343 195 3309
Fragments arranged by size:
3309 1425 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3643 3802 518
Size: 648 2118 359 159 2042
Fragments arranged by size:
2118 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2321 2401 2521 2568 2742 3419 4567 4812
Size: 715 80 120 47 174 677 1148 245
Cuts at: 4812 1606
Size: 2120
Fragments arranged by size:
2120 1148 715 677 245 174 120 80
47
NaeI GCC'GGC
Cuts at: 2153 2153
Size: 5326
NcoI C'CATG_G
Cuts at: 513 1065 2022 2274 513
Size: 552 957 252 3565
Fragments arranged by size:
3565 957 552 252
NdeI CA'TA_TG
Cuts at: 387 2044 387
Size: 1657 3669
NgoMIV G'CCGG_C
Cuts at: 2151 2151
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 4072
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2036
Size: 5326
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2678 830
Size: 1848 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
PvuII CAG'CTG
Cuts at: 2321 2521 2321
Size: 200 5126
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 5326
SacII CC_GC'GG
Cuts at: 2569 2569
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SapI GCTCTTCn'nnn_
Cuts at: 2456 2456
Size: 5326
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2674 3809 4490 4681 826
Size: 219 1629 1135 681 191 1471
Fragments arranged by size:
1629 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3430 4331 4593 820
Size: 197 926 246 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 2623 3117 493
Size: 2130 494 2702
Fragments arranged by size:
2702 2130 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 1517
Size: 750 4576
StyI C'CwwG_G
Cuts at: 513 1065 2022 2274 2487 2765 513
Size: 552 957 252 213 278 3074
Fragments arranged by size:
3074 957 552 278 252 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2144 2408 3353 3370 3523 3708 4153
Size: 1255 564 264 945 17 153 185 445
Cuts at: 4153 325
Size: 1498
Fragments arranged by size:
1498 1255 945 564 445 264 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 5286 5318 2598
Size: 32 2656 32 2606
Fragments arranged by size:
2656 2606 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 3455 3455
Size: 5326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI