Restriction map for
pFN21ASDA0098
(Circular) (Six-base) MAPSORT of: pFN21ASDA0098.seq Check: 3176 from: 1 to: 6352
pFN21ASDA0098 6352 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 2951 3660 4415 5799 6067 1632
Size: 1205 114 709 755 1384 268 1917
Fragments arranged by size:
1917 1384 1205 755 709 268 114
AflII C'TTAA_G
Cuts at: 820 1017 2254 820
Size: 197 1237 4918
Fragments arranged by size:
4918 1237 197
AflIII A'CryG_T
Cuts at: 1203 2777 5251 6228 1203
Size: 1574 2474 977 1327
Fragments arranged by size:
2474 1574 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 2095 5081 2095
Size: 2986 3366
AleI CACnn'nnGTG
Cuts at: 1166 2345 1166
Size: 1179 5173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 4095 5667 4095
Size: 1572 4780
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 2873 3661 3887 4039 6148 1493
Size: 1380 788 226 152 2109 1697
Fragments arranged by size:
2109 1697 1380 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2363 3673 5128 618
Size: 325 217 627 274 302 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 302 274 217
BanII G_rGCy'C
Cuts at: 1391 2546 3066 3671 1391
Size: 1155 520 605 4072
Fragments arranged by size:
4072 1155 605 520
BbeI G_GCGC'C
Cuts at: 2065 2065
Size: 6352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2426 2460 4543 4577 1903
Size: 34 489 34 2083 34 3678
Fragments arranged by size:
3678 2083 489 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 2272 3201 1548
Size: 724 929 4699
Fragments arranged by size:
4699 929 724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4963 136
Size: 107 122 71 631 605 3291 1525
Fragments arranged by size:
3291 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3105 6347 3105
Size: 3242 3110
BlpI GC'TnA_GC
Cuts at: 3769 3769
Size: 6352
Bme1580I G_kGCm'C
Cuts at: 1790 3313 3344 4413 5569 1790
Size: 1523 31 1069 1156 2573
Fragments arranged by size:
2573 1523 1156 1069 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4083 5041 442
Size: 416 53 83 399 24 2666 958 1753
Fragments arranged by size:
2666 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 3145 3407 3651 5012 1239
Size: 1460 446 262 244 1361 2579
Fragments arranged by size:
2579 1460 1361 446 262 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2135 4477 5342 5640 2108
Size: 27 2342 865 298 2820
Fragments arranged by size:
2820 2342 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3649 4143 493
Size: 711 2445 494 2702
Fragments arranged by size:
2702 2445 711 494
BsaBI GATnn'nnATC
Cuts at: 2271 3023 6346 2271
Size: 752 3323 2277
Fragments arranged by size:
3323 2277 752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2062 4159 4541
Size: 53 83 186 936 494 35 2097 382
Cuts at: 4541 5176 275
Size: 635 1451
Fragments arranged by size:
2097 1451 936 635 494 382 186 83
53 35
BsaWI w'CCGG_w
Cuts at: 3249 4785 5183 5457 5604 6015 3249
Size: 1536 398 274 147 411 3586
Fragments arranged by size:
3586 1536 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 1178
Size: 30 165 30 934 30 5163
Fragments arranged by size:
5163 934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3201 3224 3300 1186
Size: 2015 23 76 4238
Fragments arranged by size:
4238 2015 76 23
BseYI C'CCAG_C
Cuts at: 2539 2991 3079 5555 6051 2539
Size: 452 88 2476 496 2840
Fragments arranged by size:
2840 2476 496 452 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3066 4413 4498 5569 3066
Size: 1347 85 1071 3849
Fragments arranged by size:
3849 1347 1071 85
BsmI GAATG_Cn'
Cuts at: 2403 2599 3863 3956 2403
Size: 196 1264 93 4799
Fragments arranged by size:
4799 1264 196 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2622 2622
Size: 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2546 3066 3313 3344 3671 4413 4498
Size: 399 756 520 247 31 327 742 85
Cuts at: 4498 5569 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 756 742 520 399 327 247
85 31
BspEI T'CCGG_A
Cuts at: 3249 6015 3249
Size: 2766 3586
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3707 844
Size: 2863 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2623 4239 1394
Size: 429 800 1616 3507
Fragments arranged by size:
3507 1616 800 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3097 96
Size: 3001 3351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6352
BstBI TT'CG_AA
Cuts at: 2007 5156 2007
Size: 3149 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2347 2293
Size: 54 6298
BstYI r'GATC_y
Cuts at: 1972 3105 3682 4049 4434 4451 5903 5989 6001
Size: 1133 577 367 385 17 1452 86 12
Cuts at: 6001 6347 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1133 577 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2031 3399 513
Size: 552 957 9 1368 3466
Fragments arranged by size:
3466 1368 957 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3010 487
Size: 683 56 1784 3829
Fragments arranged by size:
3829 1784 683 56
BtsI GCAGTG_nn'
Cuts at: 3155 3868 4662 4682 3155
Size: 713 794 20 4825
Fragments arranged by size:
4825 794 713 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2730 2765 529
Size: 35 2166 35 4116
Fragments arranged by size:
4116 2166 35 35
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317
Size: 54 1425 50 275 18 1969 889 1629
Cuts at: 6317 8
Size: 43
Fragments arranged by size:
1969 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 4282 1353
Size: 1521 1408 3423
Fragments arranged by size:
3423 1521 1408
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3055 3347 4939 5313 5459 387
Size: 1064 138 1466 292 1592 374 146 1280
Fragments arranged by size:
1592 1466 1280 1064 374 292 146 138
EcoICRI GAG'CTC
Cuts at: 3064 3064
Size: 6352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2699 2837 2951 3145 3407 3651 3660
Size: 393 1067 138 114 194 262 244 9
Cuts at: 3660 4415 5012 5799 6067 1239
Size: 755 597 787 268 1524
Fragments arranged by size:
1524 1067 787 755 597 393 268 262
244 194 138 114 9
EcoO109I rG'GnC_Cy
Cuts at: 2037 2119 3002 2037
Size: 82 883 5387
Fragments arranged by size:
5387 883 82
EcoRI G'AATT_C
Cuts at: 3661 4039 3661
Size: 378 5974
EcoRV GAT'ATC
Cuts at: 3303 3573 3303
Size: 270 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 6352
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 2065 5499 1678
Size: 387 3434 2531
Fragments arranged by size:
3434 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2337 2369 2813
Size: 32 135 32 509 32 224 32 444
Cuts at: 2813 2845 3027 3059 4993 5025 5067 5099 1373
Size: 32 182 32 1934 32 42 32 2626
Fragments arranged by size:
2626 1934 509 444 224 182 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2494 2557 2635 3696 3942 669
Size: 988 281 556 63 78 1061 246 3079
Fragments arranged by size:
3079 1061 988 556 281 246 78 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6352
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KasI G'GCGC_C
Cuts at: 2061 2061
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 2387 3951 2387
Size: 1564 4788
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5466 5650 1305
Size: 4161 184 2007
Fragments arranged by size:
4161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3398 4310 4669 4828 518
Size: 648 1179 1053 912 359 159 2042
Fragments arranged by size:
2042 1179 1053 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3768 4445 5593 5838 1606
Size: 2162 677 1148 245 2120
Fragments arranged by size:
2162 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2062 2062
Size: 6352
NcoI C'CATG_G
Cuts at: 513 1065 2022 2031 3399 513
Size: 552 957 9 1368 3466
Fragments arranged by size:
3466 1368 957 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2781 3539 3710 5255 1132
Size: 97 1552 758 171 1545 2229
Fragments arranged by size:
2229 1552 1545 758 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 2777 5251 2777
Size: 2474 3878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 3002 2037
Size: 965 5387
PshAI GACnn'nnGTC
Cuts at: 1613 2831 3449 1613
Size: 1218 618 4516
Fragments arranged by size:
4516 1218 618
PsiI TTA'TAA
Cuts at: 2844 3922 2844
Size: 1078 5274
PstI C_TGCA'G
Cuts at: 830 3704 830
Size: 2874 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SacI G_AGCT'C
Cuts at: 3066 3066
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SanDI GG'GwC_CC
Cuts at: 2037 2037
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 2151 2607 3700 4835 5516 5707 826
Size: 219 1106 456 1093 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 1093 681 456 219 191
SfoI GGC'GCC
Cuts at: 2063 2063
Size: 6352
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 2123 2254 4456 5357 5619
Size: 197 926 171 9 131 2202 901 262
Cuts at: 5619 820
Size: 1553
Fragments arranged by size:
2202 1553 926 901 262 197 171 131
9
SnaBI TAC'GTA
Cuts at: 493 3649 4143 493
Size: 3156 494 2702
Fragments arranged by size:
3156 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6352
StyI C'CwwG_G
Cuts at: 513 1065 2022 2031 3399 3791 513
Size: 552 957 9 1368 392 3074
Fragments arranged by size:
3074 1368 957 552 392 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2412 2424 2745 2809 3461 4379 4396
Size: 1255 832 12 321 64 652 918 17
Cuts at: 4396 4549 4734 5179 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 918 832 652 445 321 185
153 64 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3097 4598 6310
Size: 274 80 33 51 1443 1120 1501 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
1712 1501 1443 1120 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6312 6344 6312
Size: 32 6320
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2293 2029
Size: 264 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SrfI SwaI