Restriction map for
pFN21ASDA0091
(Circular) (Six-base) MAPSORT of: pFN21ASDA0091.seq Check: 9878 from: 1 to: 7885
pFN21ASDA0091 7885 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2774 3275 2774
Size: 501 7384
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5695 278
Size: 53 83 186 936 4159 2468
Fragments arranged by size:
4159 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2268 5228 1937
Size: 331 2960 4594
Fragments arranged by size:
4594 2960 331
Acc65I G'GTAC_C
Cuts at: 5206 5206
Size: 7885
AclI AA'CG_TT
Cuts at: 1563 6012 6385 1563
Size: 4449 373 3063
Fragments arranged by size:
4449 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 4155 4174 5948 7332 7600 1632
Size: 641 1882 19 1774 1384 268 1917
Fragments arranged by size:
1917 1882 1774 1384 641 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7688
AflIII A'CryG_T
Cuts at: 1203 2928 3393 3510 4070 4374 6784 7761 1203
Size: 1725 465 117 560 304 2410 977 1327
Fragments arranged by size:
2410 1725 1327 977 560 465 304 117
AgeI A'CCGG_T
Cuts at: 6716 6716
Size: 7885
AhdI GACnn_n'nnGTC
Cuts at: 3501 6614 3501
Size: 3113 4772
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 1184
Size: 1407 6478
AlwNI CAG_nnn'CTG
Cuts at: 2611 3290 3358 4024 4197 5628 7200 2611
Size: 679 68 666 173 1431 1572 3296
Fragments arranged by size:
3296 1572 1431 679 666 173 68
ApaI G_GGCC'C
Cuts at: 3250 4063 3250
Size: 813 7072
ApaLI G'TGCA_C
Cuts at: 5942 7098 5942
Size: 1156 6729
ApoI r'AATT_y
Cuts at: 1493 2159 2230 5194 5420 5572 7681 1493
Size: 666 71 2964 226 152 2109 1697
Fragments arranged by size:
2964 2109 1697 666 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 6439 160
Size: 872 5407 1606
Fragments arranged by size:
5407 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7885
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 4055 5210 1870
Size: 73 1440 672 1155 4545
Fragments arranged by size:
4545 1440 1155 672 73
BamHI G'GATC_C
Cuts at: 5215 5582 5215
Size: 367 7518
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3240 4846 4964 5206 6661
Size: 325 217 627 1453 1606 118 242 1455
Cuts at: 6661 618
Size: 1842
Fragments arranged by size:
1842 1606 1455 1453 627 325 242 217
118
BanII G_rGCy'C
Cuts at: 1391 2534 3250 3479 4063 5204 1391
Size: 1143 716 229 584 1141 4072
Fragments arranged by size:
4072 1143 1141 716 584 229
BbeI G_GCGC'C
Cuts at: 4850 4968 4850
Size: 118 7767
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6914
BbvCI CC'TCA_GC
Cuts at: 2939 4527 2939
Size: 1588 6297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6076 6110 1903
Size: 34 4139 34 3678
Fragments arranged by size:
4139 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2201 4261 5777 6987 2201
Size: 2060 1516 1210 3099
Fragments arranged by size:
3099 2060 1516 1210
BclI T'GATC_A
Cuts at: 1548 3073 3774 3985 1548
Size: 1525 701 211 5448
Fragments arranged by size:
5448 1525 701 211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3622 6496 136
Size: 107 122 71 631 605 1950 2874 1525
Fragments arranged by size:
2874 1950 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3490 7880 3490
Size: 4390 3495
BlpI GC'TnA_GC
Cuts at: 2684 5302 2684
Size: 2618 5267
Bme1580I G_kGCm'C
Cuts at: 1790 2251 3250 4063 5946 7102 1790
Size: 461 999 813 1883 1156 2573
Fragments arranged by size:
2573 1883 1156 999 813 461
BmgBI CAC'GTC
Cuts at: 1318 2501 2929 4162 1318
Size: 1183 428 1233 5041
Fragments arranged by size:
5041 1233 1183 428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 4683 4845
Size: 416 53 83 399 24 1271 1995 162
Cuts at: 4845 5616 6574 442
Size: 771 958 1753
Fragments arranged by size:
1995 1753 1271 958 771 416 399 162
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7885
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 7885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2808 3258 4607 4695 4778 4974 5001 6545
Size: 1569 450 1349 88 83 196 27 1544
Cuts at: 6545 1239
Size: 2579
Fragments arranged by size:
2579 1569 1544 1349 450 196 88 83
27
Bpu10I CC'TnA_GC
Cuts at: 2939 3759 4527 2939
Size: 820 768 6297
Fragments arranged by size:
6297 820 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 4917 6010 6875 7173 2414
Size: 2503 1093 865 298 3126
Fragments arranged by size:
3126 2503 1093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4767 6548 882
Size: 617 3268 1781 2219
Fragments arranged by size:
3268 2219 1781 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 5182 5676 493
Size: 711 2867 1111 494 2702
Fragments arranged by size:
2867 2702 1111 711 494
BsaBI GATnn'nnATC
Cuts at: 7879 7879
Size: 7885
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2895 4422 4847 4965
Size: 53 83 186 936 1362 1527 425 118
Cuts at: 4965 5692 6074 6709 275
Size: 727 382 635 1451
Fragments arranged by size:
1527 1451 1362 936 727 635 425 382
186 118 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4271 6318 6716 6990 7137 7548 4152
Size: 119 2047 398 274 147 411 4489
Fragments arranged by size:
4489 2047 411 398 274 147 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5040 5070 1178
Size: 30 165 30 3637 30 3993
Fragments arranged by size:
3993 3637 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3667 4133 4136 4290 4610 1186
Size: 2481 466 3 154 320 4461
Fragments arranged by size:
4461 2481 466 320 154 3
BseYI C'CCAG_C
Cuts at: 3479 3528 3790 4288 7088 7584 3479
Size: 49 262 498 2800 496 3780
Fragments arranged by size:
3780 2800 498 496 262 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3837 4414 1163
Size: 573 2101 577 4634
Fragments arranged by size:
4634 2101 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 4922 5335 6096 7124 1540
Size: 275 186 1177 1744 413 761 1028 2301
Fragments arranged by size:
2301 1744 1177 1028 761 413 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 3311 5946 6031 7102 2628
Size: 683 2635 85 1071 3411
Fragments arranged by size:
3411 2635 1071 683 85
BsmI GAATG_Cn'
Cuts at: 2560 3555 5396 5489 2560
Size: 995 1841 93 4956
Fragments arranged by size:
4956 1841 995 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4835 4835
Size: 7885
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2251 2534 2628 3250 3311 3479 4063
Size: 399 461 283 94 622 61 168 584
Cuts at: 4063 5204 5946 6031 7102 1391
Size: 1141 742 85 1071 2174
Fragments arranged by size:
2174 1141 1071 742 622 584 461 399
283 168 94 85 61
BspEI T'CCGG_A
Cuts at: 4152 7548 4152
Size: 3396 4489
BspHI T'CATG_A
Cuts at: 3771 5774 7504 3771
Size: 2003 1730 4152
Fragments arranged by size:
4152 2003 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2774 3275 5140 5240 844
Size: 1930 501 1865 100 3489
Fragments arranged by size:
3489 1930 1865 501 100
BsrBI CCG'CTC
Cuts at: 1394 1823 4962 5772 1394
Size: 429 3139 810 3507
Fragments arranged by size:
3507 3139 810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 3040 3753 6380 6554 65
Size: 1183 1222 570 713 2627 174 1396
Fragments arranged by size:
2627 1396 1222 1183 713 570 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6529 6716 1700
Size: 110 4719 187 2869
Fragments arranged by size:
4719 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7885
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7885
BssSI C'ACGA_G
Cuts at: 2054 5945 6957 2054
Size: 3891 1012 2982
Fragments arranged by size:
3891 2982 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5143 794
Size: 4349 3536
BstBI TT'CG_AA
Cuts at: 2007 6689 2007
Size: 4682 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7885
BstXI CCAn_nnnn'nTGG
Cuts at: 4570 4756 4891 4570
Size: 186 135 7564
Fragments arranged by size:
7564 186 135
BstYI r'GATC_y
Cuts at: 1972 3441 3490 5215 5582 5967 5984 7436 7522
Size: 1469 49 1725 367 385 17 1452 86
Cuts at: 7522 7534 7880 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1725 1469 1452 385 367 346 86
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2767 3409 5052 513
Size: 552 1702 642 1643 3346
Fragments arranged by size:
3346 1702 1643 642 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3106 4347 4384 487
Size: 683 56 1880 1241 37 3988
Fragments arranged by size:
3988 1880 1241 683 56 37
BtsI GCAGTG_nn'
Cuts at: 2266 2609 4259 5401 6195 6215 2266
Size: 343 1650 1142 794 20 3936
Fragments arranged by size:
3936 1650 1142 794 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2157 2192 529
Size: 35 1593 35 6222
Fragments arranged by size:
6222 1593 35 35
DraI TTT'AAA
Cuts at: 2916 4467 4836 5189 2916
Size: 1551 369 353 5612
Fragments arranged by size:
5612 1551 369 353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6892 809
Size: 507 5576 1802
Fragments arranged by size:
5576 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3708 4362 4996
Size: 54 1425 50 275 18 1878 654 634
Cuts at: 4996 5332 6221 7850 8
Size: 336 889 1629 43
Fragments arranged by size:
1878 1629 1425 889 654 634 336 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5332 1537
Size: 275 3520 4090
Fragments arranged by size:
4090 3520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2506 4131 5815 1353
Size: 896 257 1625 1684 3423
Fragments arranged by size:
3423 1684 1625 896 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6472 6846 6992 387
Size: 1064 138 4883 374 146 1280
Fragments arranged by size:
4883 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2808 3258 4155 4174 4607 4695
Size: 393 641 535 450 897 19 433 88
Cuts at: 4695 4778 4974 5001 5948 6545 7332 7600 1239
Size: 83 196 27 947 597 787 268 1524
Fragments arranged by size:
1524 947 897 787 641 597 535 450
433 393 268 196 88 83 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 4895 4895
Size: 7885
EcoO109I rG'GnC_Cy
Cuts at: 2119 3247 3355 4051 4059 4060 4941 2119
Size: 1128 108 696 8 1 881 5063
Fragments arranged by size:
5063 1128 881 696 108 8 1
EcoRI G'AATT_C
Cuts at: 2159 5194 5572 2159
Size: 3035 378 4472
Fragments arranged by size:
4472 3035 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4615 4615
Size: 7885
FspI TGC'GCA
Cuts at: 6391 6391
Size: 7885
HaeII r_GCGC'y
Cuts at: 1678 4684 4850 4968 5134 7032 1678
Size: 3006 166 118 166 1898 2531
Fragments arranged by size:
3006 2531 1898 166 166 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3487 3519 3788 3820 4269
Size: 32 135 32 1915 32 269 32 449
Cuts at: 4269 4292 4301 4324 6526 6558 6600 6632 1373
Size: 23 9 23 2202 32 42 32 2626
Fragments arranged by size:
2626 2202 1915 449 269 135 42 32
32 32 32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 2980 3391 5229 5475 669
Size: 988 281 97 945 411 1838 246 3079
Fragments arranged by size:
3079 1838 988 945 411 281 246 97
HindIII A'AGCT_T
Cuts at: 748 2027 748
Size: 1279 6606
HpaI GTT'AAC
Cuts at: 5475 5475
Size: 7885
KasI G'GCGC_C
Cuts at: 4846 4964 4846
Size: 118 7767
KpnI G_GTAC'C
Cuts at: 5210 5210
Size: 7885
MfeI C'AATT_G
Cuts at: 2272 4564 5484 2272
Size: 2292 920 4673
Fragments arranged by size:
4673 2292 920
MluI A'CGCG_T
Cuts at: 7761 7761
Size: 7885
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6999 7183 1305
Size: 5694 184 2007
Fragments arranged by size:
5694 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3710 4364 10
Size: 54 1425 343 1878 654 3531
Fragments arranged by size:
3531 1878 1425 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3722 3851 5843 6202 6361 518
Size: 648 1611 945 129 1992 359 159 2042
Fragments arranged by size:
2042 1992 1611 945 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 4526 5301 5978 7126 7371 1606
Size: 2920 775 677 1148 245 2120
Fragments arranged by size:
2920 2120 1148 775 677 245
NarI GG'CG_CC
Cuts at: 4847 4965 4847
Size: 118 7767
NcoI C'CATG_G
Cuts at: 513 1065 2767 5052 513
Size: 552 1702 2285 3346
Fragments arranged by size:
3346 2285 1702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7885
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7885
NotI GC'GGCC_GC
Cuts at: 5332 5332
Size: 7885
NruI TCG'CGA
Cuts at: 4403 4403
Size: 7885
NspI r_CATG'y
Cuts at: 1132 1229 2549 3397 3514 4378 5243 6788 1132
Size: 97 1320 848 117 864 865 1545 2229
Fragments arranged by size:
2229 1545 1320 865 864 848 117 97
PasI CC'CwG_GG
Cuts at: 1348 2527 3939 5121 1348
Size: 1179 1412 1182 4112
Fragments arranged by size:
4112 1412 1182 1179
PciI A'CATG_T
Cuts at: 3393 3510 4374 6784 3393
Size: 117 864 2410 4494
Fragments arranged by size:
4494 2410 864 117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2129 4418 4456 5014 1108
Size: 1021 2289 38 558 3979
Fragments arranged by size:
3979 2289 1021 558 38
PfoI T'CCnGG_A
Cuts at: 4978 4978
Size: 7885
PmeI GTTT'AAAC
Cuts at: 5189 5189
Size: 7885
PmlI CAC'GTG
Cuts at: 4071 4071
Size: 7885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5954 5986 7467 7499 5954
Size: 32 1481 32 6340
Fragments arranged by size:
6340 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4051 4051
Size: 7885
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7885
PsiI TTA'TAA
Cuts at: 5455 5455
Size: 7885
PspOMI G'GGCC_C
Cuts at: 3246 4059 3246
Size: 813 7072
PstI C_TGCA'G
Cuts at: 830 2616 5237 830
Size: 1786 2621 3478
Fragments arranged by size:
3478 2621 1786
PvuI CG_AT'CG
Cuts at: 2001 4922 2001
Size: 2921 4964
PvuII CAG'CTG
Cuts at: 4526 4526
Size: 7885
SalI G'TCGA_C
Cuts at: 1936 5227 1936
Size: 3291 4594
SapI GCTCTTCn'nnn_
Cuts at: 2249 2506 2249
Size: 257 7628
SbfI CC_TGCA'GG
Cuts at: 5237 5237
Size: 7885
ScaI AGT'ACT
Cuts at: 4127 6133 7845 4127
Size: 2006 1712 4167
Fragments arranged by size:
4167 2006 1712
SfcI C'TryA_G
Cuts at: 826 1045 2612 5233 6368 7049 7240 826
Size: 219 1567 2621 1135 681 191 1471
Fragments arranged by size:
2621 1567 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 4848 4966 4848
Size: 118 7767
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7885
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7885
SmaI CCC'GGG
Cuts at: 1872 3385 5212 1872
Size: 1513 1827 4545
Fragments arranged by size:
4545 1827 1513
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 4932 5989 6890 7152 820
Size: 197 926 486 2503 1057 901 262 1553
Fragments arranged by size:
2503 1553 1057 926 901 486 262 197
SnaBI TAC'GTA
Cuts at: 493 5182 5676 493
Size: 4689 494 2702
Fragments arranged by size:
4689 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7885
SphI G_CATG'C
Cuts at: 1132 2549 5243 1132
Size: 1417 2694 3774
Fragments arranged by size:
3774 2694 1417
SspI AAT'ATT
Cuts at: 5 52 5809 5
Size: 47 5757 2081
Fragments arranged by size:
5757 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7885
StyI C'CwwG_G
Cuts at: 513 1065 2767 2812 3692 4450 4749 5052 5324
Size: 552 1702 45 880 758 299 303 272
Cuts at: 5324 513
Size: 3074
Fragments arranged by size:
3074 1702 880 758 552 303 299 272
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3670 5912 5929 6082 6267 6712 325
Size: 1255 2090 2242 17 153 185 445 1498
Fragments arranged by size:
2242 2090 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4125 4546 4626
Size: 274 80 33 51 1443 2148 421 80
Cuts at: 4626 6131 7843 96
Size: 1505 1712 138
Fragments arranged by size:
2148 1712 1505 1443 421 274 138 80
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5040 5072 7845 7877 5040
Size: 32 2773 32 5048
Fragments arranged by size:
5048 2773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3194 3802 1316
Size: 1878 608 5399
Fragments arranged by size:
5399 1878 608
XbaI T'CTAG_A
Cuts at: 5221 5221
Size: 7885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2524 4812 5059 2524
Size: 2288 247 5350
Fragments arranged by size:
5350 2288 247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7885
XmaI C'CCGG_G
Cuts at: 1870 3383 5210 1870
Size: 1513 1827 4545
Fragments arranged by size:
4545 1827 1513
XmnI GAAnn'nnTTC
Cuts at: 2560 6014 2560
Size: 3454 4431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5693 276
Size: 53 83 186 936 4159 2468
Fragments arranged by size:
4159 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI