Restriction map for
pFN21ASDA0085
(Circular) (Six-base) MAPSORT of: pFN21ASDA0085.seq Check: 4162 from: 1 to: 5515
pFN21ASDA0085 5515 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 2858 1937
Size: 694 227 4594
Fragments arranged by size:
4594 694 227
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 2634 4414 5391 1203
Size: 1431 1780 977 1327
Fragments arranged by size:
1780 1431 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2446 3258 4830 2446
Size: 812 1572 3131
Fragments arranged by size:
3131 1572 812
ApaI G_GGCC'C
Cuts at: 2783 2783
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 1870
Size: 73 897 4545
Fragments arranged by size:
4545 897 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2360 2783 2834 1391
Size: 969 423 51 4072
Fragments arranged by size:
4072 969 423 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2771 928
Size: 971 872 3672
Fragments arranged by size:
3672 971 872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 2109 2586 2667 1548
Size: 561 477 81 4396
Fragments arranged by size:
4396 561 477 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2499 2783 3576 4732 1790
Size: 709 284 793 1156 2573
Fragments arranged by size:
2573 1156 793 709 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2412 1056
Size: 1356 4159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2699 4175 1239
Size: 961 499 1476 2579
Fragments arranged by size:
2579 1476 961 499
Bpu10I CC'TnA_GC
Cuts at: 2255 2459 2255
Size: 204 5311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3640 4505 4803 2369
Size: 1271 865 298 3081
Fragments arranged by size:
3081 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2812 3306 493
Size: 711 1608 494 2702
Fragments arranged by size:
2702 1608 711 494
BsaBI GATnn'nnATC
Cuts at: 2585 2588 5509 2585
Size: 3 2921 2591
Fragments arranged by size:
2921 2591 3
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2165 3322 3704 4339
Size: 53 83 186 936 632 1157 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1157 936 635 632 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 4465
Fragments arranged by size:
4465 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 2705 1186
Size: 1218 301 3996
Fragments arranged by size:
3996 1218 301
BseYI C'CCAG_C
Cuts at: 2353 2543 2782 4718 5214 2353
Size: 190 239 1936 496 2654
Fragments arranged by size:
2654 1936 496 239 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 3576 3661 4732 2139
Size: 1437 85 1071 2922
Fragments arranged by size:
2922 1437 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2360 2499 2783 2834 3576 3661
Size: 399 349 221 139 284 51 742 85
Cuts at: 3661 4732 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 284 221 139
85 51
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 2112 3404 5134 2112
Size: 1292 1730 2493
Fragments arranged by size:
2493 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2063 2304 2870 844
Size: 1219 241 566 3489
Fragments arranged by size:
3489 1219 566 241
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 2007 4319 2007
Size: 2312 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2092
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2195 2195
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2022 2155 513
Size: 552 957 133 3873
Fragments arranged by size:
3873 957 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2062 2584 487
Size: 683 56 836 522 3418
Fragments arranged by size:
3418 836 683 522 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2962 3851
Size: 54 1425 50 275 18 264 868 889
Cuts at: 3851 5480 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 868 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2690 3445 1353
Size: 893 444 755 3423
Fragments arranged by size:
3423 893 755 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 4102 4476 4622 387
Size: 1064 138 447 2066 374 146 1280
Fragments arranged by size:
2066 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2699 3578 4175 4962 5230 1239
Size: 393 568 499 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 568 499 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 4156 4188 4230
Size: 32 135 32 626 32 1926 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 1926 626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 10
Size: 54 1425 343 264 3429
Fragments arranged by size:
3429 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2492 2579 3473 3832 3991 518
Size: 648 1068 258 87 894 359 159 2042
Fragments arranged by size:
2042 1068 894 648 359 258 159 87
MspA1I CmG'CkG
Cuts at: 1606 2443 2931 3608 4756 5001 1606
Size: 837 488 677 1148 245 2120
Fragments arranged by size:
2120 1148 837 677 488 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2155 513
Size: 552 957 133 3873
Fragments arranged by size:
3873 957 552 133
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 2408 1052
Size: 1356 4159
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2638 2873 4418 1132
Size: 97 1409 235 1545 2229
Fragments arranged by size:
2229 1545 1409 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 2634 4414 2634
Size: 1780 3735
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2722 1108
Size: 1614 3901
PfoI T'CCnGG_A
Cuts at: 2396 2396
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2779 2779
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2442 2480 2867 830
Size: 1612 38 387 3478
Fragments arranged by size:
3478 1612 387 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2443 2443
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SapI GCTCTTCn'nnn_
Cuts at: 2246 2246
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2438 2476 2659 2863 3998 4679 4870
Size: 219 1393 38 183 204 1135 681 191
Cuts at: 4870 826
Size: 1471
Fragments arranged by size:
1471 1393 1135 681 219 204 191 183
38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3619 4520 4782 820
Size: 197 926 441 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 2812 3306 493
Size: 2319 494 2702
Fragments arranged by size:
2702 2319 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2022 2155 2954 513
Size: 552 957 133 799 3074
Fragments arranged by size:
3074 957 799 552 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI