Restriction map for
pFN21ASDA0080
(Circular) (Six-base) MAPSORT of: pFN21ASDA0080.seq Check: 632 from: 1 to: 6022
pFN21ASDA0080 6022 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3030 3365 1937
Size: 1093 335 4594
Fragments arranged by size:
4594 1093 335
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5469 5737 1632
Size: 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 2065 2993 4853 2065
Size: 928 1860 3234
Fragments arranged by size:
3234 1860 928
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AlwNI CAG_nnn'CTG
Cuts at: 2324 2740 3765 5337 2324
Size: 416 1025 1572 3009
Fragments arranged by size:
3009 1572 1025 416
ApaLI G'TGCA_C
Cuts at: 3079 4079 5235 3079
Size: 1000 1156 3866
Fragments arranged by size:
3866 1156 1000
ApoI r'AATT_y
Cuts at: 1493 3331 3557 3709 5818 1493
Size: 1838 226 152 2109 1697
Fragments arranged by size:
2109 1838 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2781 3347 1870
Size: 73 838 566 4545
Fragments arranged by size:
4545 838 566 73
BamHI G'GATC_C
Cuts at: 2498 3352 3719 2498
Size: 854 367 4801
Fragments arranged by size:
4801 854 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 3343 4798 618
Size: 325 217 627 1022 534 1455 1842
Fragments arranged by size:
1842 1455 1022 627 534 325 217
BanII G_rGCy'C
Cuts at: 1391 2426 2907 3275 3341 1391
Size: 1035 481 368 66 4072
Fragments arranged by size:
4072 1035 481 368 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2660 2678 928
Size: 971 761 18 4272
Fragments arranged by size:
4272 971 761 18
BbvCI CC'TCA_GC
Cuts at: 2228 2762 2228
Size: 534 5488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 2333 3439 2333
Size: 1106 4916
Bme1580I G_kGCm'C
Cuts at: 1790 3083 3223 4083 5239 1790
Size: 1293 140 860 1156 2573
Fragments arranged by size:
2573 1293 1156 860 140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2921
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2891 3274 4682 1239
Size: 1221 431 383 1408 2579
Fragments arranged by size:
2579 1408 1221 431 383
Bpu10I CC'TnA_GC
Cuts at: 2228 2762 2228
Size: 534 5488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4147 5012 5310 4147
Size: 865 298 4859
Fragments arranged by size:
4859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 2690 3002 4685 882
Size: 617 717 474 312 1683 2219
Fragments arranged by size:
2219 1683 717 617 474 312
BsaAI yAC'GTr
Cuts at: 493 1204 3319 3813 493
Size: 711 2115 494 2702
Fragments arranged by size:
2702 2115 711 494
BsaBI GATnn'nnATC
Cuts at: 2987 6016 2987
Size: 3029 2993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2065 2993 4455 4853 5127 5274 5685 2065
Size: 928 1462 398 274 147 411 2402
Fragments arranged by size:
2402 1462 928 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2272 2302 2449 2479 1178
Size: 30 165 30 869 30 147 30 4721
Fragments arranged by size:
4721 869 165 147 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 1186
Size: 1309 4713
BseYI C'CCAG_C
Cuts at: 2831 5225 5721 2831
Size: 2394 496 3132
Fragments arranged by size:
3132 2394 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2732 1163
Size: 573 996 4453
Fragments arranged by size:
4453 996 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3472 4233 5261 1540
Size: 275 186 1177 294 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2907 3083 4083 4168 5239 2907
Size: 176 1000 85 1071 3690
Fragments arranged by size:
3690 1071 1000 176 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2426 2907 3083 3223 3275 3341 4083
Size: 399 636 481 176 140 52 66 742
Cuts at: 4083 4168 5239 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 481 399 176 140
85 66 52
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3377 844
Size: 2533 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3909 1394
Size: 429 2086 3507
Fragments arranged by size:
3507 2086 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3048 4517 4691 65
Size: 1183 1800 1469 174 1396
Fragments arranged by size:
1800 1469 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2075 2993 4666 4853 1700
Size: 110 255 10 918 1673 187 2869
Fragments arranged by size:
2869 1673 918 255 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 2007 4826 2007
Size: 2819 3203
BstEII G'GTnAC_C
Cuts at: 1184 2116 1184
Size: 932 5090
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2398
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2347 2369 2498 2600 3352 3719 4104 4121
Size: 375 22 129 102 752 367 385 17
Cuts at: 4121 5573 5659 5671 6017 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 752 385 375 367 346 129
102 86 22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2566 513
Size: 552 957 544 3969
Fragments arranged by size:
3969 957 552 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 3538 4332 4352 3538
Size: 794 20 5208
Fragments arranged by size:
5208 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DraIII CAC_nnn'GTG
Cuts at: 3227 3227
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 3288 3469
Size: 54 1425 50 275 18 243 1215 181
Cuts at: 3469 4358 5987 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1215 889 275 243 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3952 1353
Size: 2599 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2905 2905
Size: 6022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2891 3274 4085 4682 5469 5737
Size: 393 828 431 383 811 597 787 268
Cuts at: 5737 1239
Size: 1524
Fragments arranged by size:
1524 828 811 787 597 431 393 383
268
EcoO109I rG'GnC_Cy
Cuts at: 2088 2357 2737 2088
Size: 269 380 5373
Fragments arranged by size:
5373 380 269
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
EcoRV GAT'ATC
Cuts at: 2989 3145 2989
Size: 156 5866
FseI GG_CCGG'CC
Cuts at: 2079 2079
Size: 6022
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 5169 1678
Size: 3491 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 4663 4695 4737
Size: 32 135 32 1190 32 1869 32 42
Cuts at: 4737 4769 1373
Size: 32 2626
Fragments arranged by size:
2626 1869 1190 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 5136 5320 1305
Size: 763 3068 184 2007
Fragments arranged by size:
3068 2007 763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3290 10
Size: 54 1425 343 1458 2742
Fragments arranged by size:
2742 1458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2396 2618 3980 4339 4498 518
Size: 648 1086 144 222 1362 359 159 2042
Fragments arranged by size:
2042 1362 1086 648 359 222 159 144
MspA1I CmG'CkG
Cuts at: 1606 2731 3438 4115 5263 5508 1606
Size: 1125 707 677 1148 245 2120
Fragments arranged by size:
2120 1148 1125 707 677 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 6022
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4513
Fragments arranged by size:
4513 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2197 3380 4925 1132
Size: 97 968 1183 1545 2229
Fragments arranged by size:
2229 1545 1183 968 97
PasI CC'CwG_GG
Cuts at: 1348 2352 2444 2596 1348
Size: 1004 92 152 4774
Fragments arranged by size:
4774 1004 152 92
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6022
PfoI T'CCnGG_A
Cuts at: 2556 2556
Size: 6022
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2737 2737
Size: 6022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2162 2194 3017 3049 2162
Size: 32 823 32 5135
Fragments arranged by size:
5135 823 32 32
PstI C_TGCA'G
Cuts at: 830 2736 3374 830
Size: 1906 638 3478
Fragments arranged by size:
3478 1906 638
PvuI CG_AT'CG
Cuts at: 2001 3178 2001
Size: 1177 4845
PvuII CAG'CTG
Cuts at: 2731 2731
Size: 6022
SacI G_AGCT'C
Cuts at: 2907 2907
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 3314 4270 5982 3314
Size: 956 1712 3354
Fragments arranged by size:
3354 1712 956
SexAI A'CCwGG_T
Cuts at: 2739 2739
Size: 6022
SfcI C'TryA_G
Cuts at: 826 1045 2722 2732 3370 4505 5186 5377 826
Size: 219 1677 10 638 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 638 219 191 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 2065 1700
Size: 365 5657
SmaI CCC'GGG
Cuts at: 1872 3349 1872
Size: 1477 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4126 5027 5289 820
Size: 197 926 2183 901 262 1553
Fragments arranged by size:
2183 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3319 3813 493
Size: 2826 494 2702
Fragments arranged by size:
2826 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2022 3461 513
Size: 552 957 1439 3074
Fragments arranged by size:
3074 1439 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3312 4268 5980
Size: 274 80 33 51 1443 1335 956 1712
Cuts at: 5980 96
Size: 138
Fragments arranged by size:
1712 1443 1335 956 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2272 2304 5982 6014 2272
Size: 32 3678 32 2280
Fragments arranged by size:
3678 2280 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3193 3193
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 3347 1870
Size: 1477 4545
XmnI GAAnn'nnTTC
Cuts at: 4151 4151
Size: 6022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI