Restriction map for
pFN21ASDA0074
(Circular) (Six-base) MAPSORT of: pFN21ASDA0074.seq Check: 927 from: 1 to: 6952
pFN21ASDA0074 6952 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2550 3042 4295 1937
Size: 613 492 1253 4594
Fragments arranged by size:
4594 1253 613 492
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2094 3105 3983 5015 6399 6667 1632
Size: 423 39 1011 878 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1032 1011 878 423 268 39
AfeI AGC'GCT
Cuts at: 3993 3993
Size: 6952
AflII C'TTAA_G
Cuts at: 820 1017 3614 820
Size: 197 2597 4158
Fragments arranged by size:
4158 2597 197
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 5681 5681
Size: 6952
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2964 1184
Size: 1780 5172
AlwNI CAG_nnn'CTG
Cuts at: 3198 4695 6267 3198
Size: 1497 1572 3883
Fragments arranged by size:
3883 1572 1497
ApaLI G'TGCA_C
Cuts at: 5009 6165 5009
Size: 1156 5796
ApoI r'AATT_y
Cuts at: 1493 3918 4261 4487 4639 6748 1493
Size: 2425 343 226 152 2109 1697
Fragments arranged by size:
2425 2109 1697 343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2230 2888 4277 1870
Size: 73 287 658 1389 4545
Fragments arranged by size:
4545 1389 658 287 73
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2165 3602 4273 5728
Size: 325 217 627 359 19 1437 671 1455
Cuts at: 5728 618
Size: 1842
Fragments arranged by size:
1842 1455 1437 671 627 359 325 217
19
BanII G_rGCy'C
Cuts at: 1391 3210 4092 4271 1391
Size: 1819 882 179 4072
Fragments arranged by size:
4072 1819 882 179
BbeI G_GCGC'C
Cuts at: 2150 2150
Size: 6952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 2768 3672 928
Size: 971 288 581 904 4208
Fragments arranged by size:
4208 971 904 581 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2116 2150 4084 4118 5143 5177 1903
Size: 34 179 34 1934 34 1025 34 3678
Fragments arranged by size:
3678 1934 1025 179 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4844 6054 4844
Size: 1210 5742
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5563 136
Size: 107 122 71 631 605 3891 1525
Fragments arranged by size:
3891 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2511 2964 6947 2511
Size: 453 3983 2516
Fragments arranged by size:
3983 2516 453
BlpI GC'TnA_GC
Cuts at: 3891 4082 4369 3891
Size: 191 287 6474
Fragments arranged by size:
6474 287 191
Bme1580I G_kGCm'C
Cuts at: 1790 3278 5013 6169 1790
Size: 1488 1735 1156 2573
Fragments arranged by size:
2573 1735 1488 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 4683 5641
Size: 416 53 83 399 24 748 2518 958
Cuts at: 5641 442
Size: 1753
Fragments arranged by size:
2518 1753 958 748 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 4234 2903
Size: 1331 5621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 3318 3822 5612 1239
Size: 1033 1046 504 1790 2579
Fragments arranged by size:
2579 1790 1046 1033 504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5077 5942 6240 5077
Size: 865 298 5789
Fragments arranged by size:
5789 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5615 882
Size: 617 4116 2219
Fragments arranged by size:
4116 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4249 4743 493
Size: 711 3045 494 2702
Fragments arranged by size:
3045 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 4759 5141 5776
Size: 53 83 186 936 614 2612 382 635
Cuts at: 5776 275
Size: 1451
Fragments arranged by size:
2612 1451 936 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3998 5385 5783 6057 6204 6615 3998
Size: 1387 398 274 147 411 4335
Fragments arranged by size:
4335 1387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 3680 3710 4233
Size: 30 165 30 681 30 1566 30 523
Cuts at: 4233 4263 1178
Size: 30 3867
Fragments arranged by size:
3867 1566 681 523 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 4123 1186
Size: 906 2031 4015
Fragments arranged by size:
4015 2031 906
BseYI C'CCAG_C
Cuts at: 2038 2569 6155 6651 2038
Size: 531 3586 496 2339
Fragments arranged by size:
3586 2339 531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2806 2970 1163
Size: 573 1070 164 5145
Fragments arranged by size:
5145 1070 573 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5013 5098 6169 5013
Size: 85 1071 5796
Fragments arranged by size:
5796 1071 85
BsmI GAATG_Cn'
Cuts at: 2407 4463 4556 2407
Size: 2056 93 4803
Fragments arranged by size:
4803 2056 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2069
Size: 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3210 3278 4092 4271 5013 5098 6169
Size: 399 1420 68 814 179 742 85 1071
Cuts at: 6169 1391
Size: 2174
Fragments arranged by size:
2174 1420 1071 814 742 399 179 85
68
BspEI T'CCGG_A
Cuts at: 3998 6615 3998
Size: 2617 4335
BspHI T'CATG_A
Cuts at: 3181 4841 6571 3181
Size: 1660 1730 3562
Fragments arranged by size:
3562 1730 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4110 4307 844
Size: 3266 197 3489
Fragments arranged by size:
3489 3266 197
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 4839 1394
Size: 429 389 2627 3507
Fragments arranged by size:
3507 2627 429 389
BsrDI GCAATG_nn'
Cuts at: 65 1248 5447 5621 65
Size: 1183 4199 174 1396
Fragments arranged by size:
4199 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5596 5783 1700
Size: 110 3786 187 2869
Fragments arranged by size:
3786 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6952
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 5012 6024 5012
Size: 1012 5940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6952
BstBI TT'CG_AA
Cuts at: 2007 5756 2007
Size: 3749 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 3946 4150 3946
Size: 204 6748
BstYI r'GATC_y
Cuts at: 1972 2511 2964 4282 4649 5034 5051 6503 6589
Size: 539 453 1318 367 385 17 1452 86
Cuts at: 6589 6601 6947 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1318 539 453 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2551 2551
Size: 6952
BtgI C'CryG_G
Cuts at: 513 1065 2022 2874 513
Size: 552 957 852 4591
Fragments arranged by size:
4591 957 852 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 2818 2951 4468 5262 5282 2042
Size: 776 133 1517 794 20 3712
Fragments arranged by size:
3712 1517 794 776 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 2476 2928 4256 2476
Size: 452 1328 5172
Fragments arranged by size:
5172 1328 452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3578 5959 809
Size: 507 2262 2381 1802
Fragments arranged by size:
2381 2262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2979 4399 5288
Size: 54 1425 50 275 18 1149 1420 889
Cuts at: 5288 6917 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 1149 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2071 3180 3407 4882 1353
Size: 718 1109 227 1475 3423
Fragments arranged by size:
3423 1475 1109 718 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5539 5913 6059 387
Size: 1064 138 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2094 2272 3105 3318 3822 3983
Size: 393 423 39 178 833 213 504 161
Cuts at: 3983 5015 5612 6399 6667 1239
Size: 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 833 787 597 504 423 393
268 213 178 161 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2290
Size: 6952
EcoO109I rG'GnC_Cy
Cuts at: 2027 3123 3764 4099 4213 2027
Size: 1096 641 335 114 4766
Fragments arranged by size:
4766 1096 641 335 114
EcoRI G'AATT_C
Cuts at: 4261 4639 4261
Size: 378 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 6952
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 2150 2230 2936 3361 3995 6099 1678
Size: 472 80 706 425 634 2104 2531
Fragments arranged by size:
2531 2104 706 634 472 425 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2570 2602 3014
Size: 32 135 32 845 32 121 32 412
Cuts at: 3014 3046 3843 3875 5593 5625 5667 5699 1373
Size: 32 797 32 1718 32 42 32 2626
Fragments arranged by size:
2626 1718 845 797 412 135 121 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 3571 4296 4542 669
Size: 988 281 1153 480 725 246 3079
Fragments arranged by size:
3079 1153 988 725 480 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KasI G'GCGC_C
Cuts at: 2146 2146
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3723 6066 6250 1305
Size: 2418 2343 184 2007
Fragments arranged by size:
2418 2343 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5130
Fragments arranged by size:
5130 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 3332 4910 5269 5428 518
Size: 648 1378 788 1578 359 159 2042
Fragments arranged by size:
2042 1578 1378 788 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 4368 5045 6193 6438 1606
Size: 615 2147 677 1148 245 2120
Fragments arranged by size:
2147 2120 1148 677 615 245
NarI GG'CG_CC
Cuts at: 2147 2147
Size: 6952
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5443
Fragments arranged by size:
5443 957 552
NdeI CA'TA_TG
Cuts at: 387 3868 387
Size: 3481 3471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 3976 4310 5855 1132
Size: 97 2747 334 1545 2229
Fragments arranged by size:
2747 2229 1545 334 97
PasI CC'CwG_GG
Cuts at: 1348 3127 3625 1348
Size: 1779 498 4675
Fragments arranged by size:
4675 1779 498
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6952
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2105 2137 5021 5053 6534 6566 2105
Size: 32 2884 32 1481 32 2491
Fragments arranged by size:
2884 2491 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 3123 3764 4099 4213 2027
Size: 1096 641 335 114 4766
Fragments arranged by size:
4766 1096 641 335 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspXI vC'TCGA_Gb
Cuts at: 2230 2230
Size: 6952
PstI C_TGCA'G
Cuts at: 830 2220 2250 4107 4304 830
Size: 1390 30 1857 197 3478
Fragments arranged by size:
3478 1857 1390 197 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SanDI GG'GwC_CC
Cuts at: 3123 3123
Size: 6952
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SexAI A'CCwGG_T
Cuts at: 3063 3605 3063
Size: 542 6410
SfcI C'TryA_G
Cuts at: 826 1045 2216 2246 2830 3549 4103 4300 5435
Size: 219 1171 30 584 719 554 197 1135
Cuts at: 5435 6116 6307 826
Size: 681 191 1471
Fragments arranged by size:
1471 1171 1135 719 681 584 554 219
197 191 30
SfoI GGC'GCC
Cuts at: 2148 2148
Size: 6952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 4279 1872
Size: 2407 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2230 2888 3614 5056 5957 6219
Size: 197 926 287 658 726 1442 901 262
Cuts at: 6219 820
Size: 1553
Fragments arranged by size:
1553 1442 926 901 726 658 287 262
197
SnaBI TAC'GTA
Cuts at: 493 4249 4743 493
Size: 3756 494 2702
Fragments arranged by size:
3756 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4310 1132
Size: 3178 3774
SspI AAT'ATT
Cuts at: 5 52 2158 3305 4876 5
Size: 47 2106 1147 1571 2081
Fragments arranged by size:
2106 2081 1571 1147 47
StuI AGG'CCT
Cuts at: 1517 3367 1517
Size: 1850 5102
StyI C'CwwG_G
Cuts at: 513 1065 2022 2560 3247 3847 4391 513
Size: 552 957 538 687 600 544 3074
Fragments arranged by size:
3074 957 687 600 552 544 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4979 4996 5149 5334 5779 325
Size: 1255 3399 17 153 185 445 1498
Fragments arranged by size:
3399 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3416 5198 6910
Size: 274 80 33 51 1443 1439 1782 1712
Cuts at: 6910 96
Size: 138
Fragments arranged by size:
1782 1712 1443 1439 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 6912 6944 2084
Size: 32 4796 32 2092
Fragments arranged by size:
4796 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3328 3505 1316
Size: 2012 177 4763
Fragments arranged by size:
4763 2012 177
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2615 3170 3511 4034 2476
Size: 139 555 341 523 5394
Fragments arranged by size:
5394 555 523 341 139
XhoI C'TCGA_G
Cuts at: 1943 2230 2888 1943
Size: 287 658 6007
Fragments arranged by size:
6007 658 287
XmaI C'CCGG_G
Cuts at: 1870 4277 1870
Size: 2407 4545
XmnI GAAnn'nnTTC
Cuts at: 2184 5081 2184
Size: 2897 4055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SacI SacII SapI SfiI SrfI
SwaI