Restriction map for
pFN21ASDA0062
(Circular) (Six-base) MAPSORT of: pFN21ASDA0062.seq Check: 6631 from: 1 to: 6205
pFN21ASDA0062 6205 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 4268 5652 5920 1632
Size: 596 2040 1384 268 1917
Fragments arranged by size:
2040 1917 1384 596 268
AfeI AGC'GCT
Cuts at: 2140 2470 2140
Size: 330 5875
AflII C'TTAA_G
Cuts at: 820 1017 2756 820
Size: 197 1739 4269
Fragments arranged by size:
4269 1739 197
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 3131 1166
Size: 1965 4240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2206 2395 2965 3430 3948 5520 2206
Size: 189 570 465 518 1572 2891
Fragments arranged by size:
2891 1572 570 518 465 189
ApaLI G'TGCA_C
Cuts at: 2124 4262 5418 2124
Size: 2138 1156 2911
Fragments arranged by size:
2911 2138 1156
ApoI r'AATT_y
Cuts at: 1493 3514 3740 3892 6001 1493
Size: 2021 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 3530 1870
Size: 73 460 1127 4545
Fragments arranged by size:
4545 1127 460 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2914 3526 4981 618
Size: 325 217 627 758 369 612 1455 1842
Fragments arranged by size:
1842 1455 758 627 612 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 2948 3524 1391
Size: 973 584 576 4072
Fragments arranged by size:
4072 973 584 576
BbeI G_GCGC'C
Cuts at: 2918 2918
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2567 928
Size: 971 668 4566
Fragments arranged by size:
4566 971 668
BbvCI CC'TCA_GC
Cuts at: 2883 2883
Size: 6205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2480 4097 5307 2480
Size: 1617 1210 3378
Fragments arranged by size:
3378 1617 1210
BclI T'GATC_A
Cuts at: 1548 3039 1548
Size: 1491 4714
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2739 6200 2739
Size: 3461 2744
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2550 4266 5422 1790
Size: 338 422 1716 1156 2573
Fragments arranged by size:
2573 1716 1156 422 338
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4894 442
Size: 416 53 83 399 24 2519 958 1753
Fragments arranged by size:
2519 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3317 1056
Size: 2261 3944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2348 2372 3339 4865 1239
Size: 1086 23 24 967 1526 2579
Fragments arranged by size:
2579 1526 1086 967 24 23
Bpu10I CC'TnA_GC
Cuts at: 2883 2883
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2301 2498 4868 882
Size: 617 802 197 2370 2219
Fragments arranged by size:
2370 2219 802 617 197
BsaAI yAC'GTr
Cuts at: 493 1204 2266 2292 3502 3996 493
Size: 711 1062 26 1210 494 2702
Fragments arranged by size:
2702 1210 1062 711 494 26
BsaBI GATnn'nnATC
Cuts at: 3203 6199 3203
Size: 2996 3209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2915 4012 4394 5029
Size: 53 83 186 936 1382 1097 382 635
Cuts at: 5029 275
Size: 1451
Fragments arranged by size:
1451 1382 1097 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3162 3192 1178
Size: 30 165 30 1759 30 4191
Fragments arranged by size:
4191 1759 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 2468 2486 2846 3164 1186
Size: 1264 18 18 360 318 4227
Fragments arranged by size:
4227 1264 360 318 18 18
BseYI C'CCAG_C
Cuts at: 2259 3194 5408 5904 2259
Size: 935 2214 496 2560
Fragments arranged by size:
2560 2214 935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2395 2978 1163
Size: 573 288 371 583 4390
Fragments arranged by size:
4390 583 573 371 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2128 2364 4266 4351 5422 2128
Size: 236 1902 85 1071 2911
Fragments arranged by size:
2911 1902 1071 236 85
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2364 2550 2948 3524 4266 4351
Size: 399 338 236 186 398 576 742 85
Cuts at: 4351 5422 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 576 399 398 338 236
186 85
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 2553 4094 5824 2553
Size: 1541 1730 2934
Fragments arranged by size:
2934 1730 1541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2973 3560 844
Size: 2129 587 3489
Fragments arranged by size:
3489 2129 587
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3278 4849 5036 1700
Size: 110 1468 1571 187 2869
Fragments arranged by size:
2869 1571 1468 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2965 794
Size: 2171 4034
BstBI TT'CG_AA
Cuts at: 2007 5009 2007
Size: 3002 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 3460 3460
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2158 2739 3486 3535 3902 4287 4304 5756
Size: 186 581 747 49 367 385 17 1452
Cuts at: 5756 5842 5854 6200 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 747 581 385 367 346 186
86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2100 2100
Size: 6205
BtgI C'CryG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3799
Fragments arranged by size:
3799 1854 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 2703 2887 2902 3721 4515 4535 2316
Size: 387 184 15 819 794 20 3986
Fragments arranged by size:
3986 819 794 387 184 20 15
ClaI AT'CG_AT
Cuts at: 1554 3072 1554
Size: 1518 4687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2933 5212 809
Size: 507 1617 2279 1802
Fragments arranged by size:
2279 1802 1617 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2462 3081 3135
Size: 54 1425 50 275 18 632 619 54
Cuts at: 3135 3276 3652 4541 6170 8
Size: 141 376 889 1629 43
Fragments arranged by size:
1629 1425 889 632 619 376 275 141
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2636 3224 4135 1353
Size: 965 318 588 911 3423
Fragments arranged by size:
3423 965 911 588 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2325 2348 2372 3339 4268 4865
Size: 393 596 97 23 24 967 929 597
Cuts at: 4865 5652 5920 1239
Size: 787 268 1524
Fragments arranged by size:
1524 967 929 787 597 596 393 268
97 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3146 3146
Size: 6205
EcoO109I rG'GnC_Cy
Cuts at: 2212 2863 2962 2212
Size: 651 99 5455
Fragments arranged by size:
5455 651 99
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
EcoRV GAT'ATC
Cuts at: 3019 3019
Size: 6205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2142 2472 2918 5352 1678
Size: 464 330 446 2434 2531
Fragments arranged by size:
2531 2434 464 446 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 3028 3060 3162
Size: 32 135 32 906 32 518 32 102
Cuts at: 3162 3194 3438 3470 4846 4878 4920 4952 1373
Size: 32 244 32 1376 32 42 32 2626
Fragments arranged by size:
2626 1376 906 518 244 135 102 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KasI G'GCGC_C
Cuts at: 2914 2914
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2646 3232 5319 5503 1305
Size: 1341 586 2087 184 2007
Fragments arranged by size:
2087 2007 1341 586 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3083 3137 10
Size: 54 1425 343 1251 54 3078
Fragments arranged by size:
3078 1425 1251 343 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2229 2778 3131 3455 3458 4163
Size: 648 1006 57 549 353 324 3 705
Cuts at: 4163 4522 4681 518
Size: 359 159 2042
Fragments arranged by size:
2042 1006 705 648 549 359 353 324
159 57 3
MspA1I CmG'CkG
Cuts at: 1606 2206 2395 3621 4298 5446 5691 1606
Size: 600 189 1226 677 1148 245 2120
Fragments arranged by size:
2120 1226 1148 677 600 245 189
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 6205
NarI GG'CG_CC
Cuts at: 2915 2915
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3799
Fragments arranged by size:
3799 1854 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 3313 1052
Size: 2261 3944
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3563 5108 1132
Size: 97 2334 1545 2229
Fragments arranged by size:
2334 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2119 1348
Size: 771 5434
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 3430 1108
Size: 1287 1035 3883
Fragments arranged by size:
3883 1287 1035
PfoI T'CCnGG_A
Cuts at: 2364 2643 2364
Size: 279 5926
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PmlI CAC'GTG
Cuts at: 2266 2292 2266
Size: 26 6179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2863 2962 2863
Size: 99 6106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PstI C_TGCA'G
Cuts at: 830 2157 2202 2698 2856 2894 2970 3557 830
Size: 1327 45 496 158 38 76 587 3478
Fragments arranged by size:
3478 1327 587 496 158 76 45 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2206 2395 2206
Size: 189 6016
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 2157 2970 3557 2157
Size: 813 587 4805
Fragments arranged by size:
4805 813 587
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2153 2198 2694 2852 2890 2966 3553
Size: 219 1108 45 496 158 38 76 587
Cuts at: 3553 4688 5369 5560 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 587 496 219 191
158 76 45 38
SfoI GGC'GCC
Cuts at: 2916 2916
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 2405 3532 1872
Size: 533 1127 4545
Fragments arranged by size:
4545 1127 533
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 4309 5210 5472 820
Size: 197 926 813 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 3502 3996 493
Size: 3009 494 2702
Fragments arranged by size:
3009 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2919 3115 3644 513
Size: 552 1854 196 529 3074
Fragments arranged by size:
3074 1854 552 529 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2273 4232 4249 4402 4587 5032
Size: 1255 531 162 1959 17 153 185 445
Cuts at: 5032 325
Size: 1498
Fragments arranged by size:
1959 1498 1255 531 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4451 6163 96
Size: 274 80 33 51 1443 2474 1712 138
Fragments arranged by size:
2474 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 6165 6197 2099
Size: 32 4034 32 2107
Fragments arranged by size:
4034 2107 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3176 1316
Size: 1860 4345
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2695 2695
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 2403 3530 1870
Size: 533 1127 4545
Fragments arranged by size:
4545 1127 533
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII FseI FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI