Restriction map for
pFN21ASDA0049
(Circular) (Six-base) MAPSORT of: pFN21ASDA0049.seq Check: 497 from: 1 to: 7627
pFN21ASDA0049 7627 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3542 3542
Size: 7627
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5437 278
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 4970 1937
Size: 1449 1584 4594
Fragments arranged by size:
4594 1584 1449
Acc65I G'GTAC_C
Cuts at: 4948 4948
Size: 7627
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 2813 3164 4086 4335 4545 4782 4859
Size: 972 209 351 922 249 210 237 77
Cuts at: 4859 4868 5690 7074 7342 1632
Size: 9 822 1384 268 1917
Fragments arranged by size:
1917 1384 972 922 822 351 268 249
237 210 209 77 9
AfeI AGC'GCT
Cuts at: 2223 2223
Size: 7627
AflII C'TTAA_G
Cuts at: 820 1017 2225 820
Size: 197 1208 6222
Fragments arranged by size:
6222 1208 197
AflIII A'CryG_T
Cuts at: 1203 6526 7503 1203
Size: 5323 977 1327
Fragments arranged by size:
5323 1327 977
AgeI A'CCGG_T
Cuts at: 6458 6458
Size: 7627
AhdI GACnn_n'nnGTC
Cuts at: 3703 6356 3703
Size: 2653 4974
AleI CACnn'nnGTG
Cuts at: 1166 3767 1166
Size: 2601 5026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7627
AlwNI CAG_nnn'CTG
Cuts at: 3700 4435 4568 4742 5370 6942 3700
Size: 735 133 174 628 1572 4385
Fragments arranged by size:
4385 1572 735 628 174 133
ApaLI G'TGCA_C
Cuts at: 5684 6840 5684
Size: 1156 6471
ApoI r'AATT_y
Cuts at: 1493 2064 3006 3019 3157 4936 5162 5314 7423
Size: 571 942 13 138 1779 226 152 2109
Cuts at: 7423 1493
Size: 1697
Fragments arranged by size:
2109 1779 1697 942 571 226 152 138
13
AseI AT'TA_AT
Cuts at: 160 1032 6181 160
Size: 872 5149 1606
Fragments arranged by size:
5149 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 3432 4952 1870
Size: 73 1489 1520 4545
Fragments arranged by size:
4545 1520 1489 73
BamHI G'GATC_C
Cuts at: 2410 4957 5324 2410
Size: 2547 367 4713
Fragments arranged by size:
4713 2547 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2277 4644 4948 6403 618
Size: 325 217 627 490 2367 304 1455 1842
Fragments arranged by size:
2367 1842 1455 627 490 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2819 3044 3482 3663 4173 4496 4590 4946
Size: 1428 225 438 181 510 323 94 356
Cuts at: 4946 1391
Size: 4072
Fragments arranged by size:
4072 1428 510 438 356 323 225 181
94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 2852 3951 928
Size: 971 506 447 1099 4604
Fragments arranged by size:
4604 1099 971 506 447
BbvCI CC'TCA_GC
Cuts at: 4110 4353 4110
Size: 243 7384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2406 2440 5818 5852 1903
Size: 34 469 34 3378 34 3678
Fragments arranged by size:
3678 3378 469 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2200 5519 6729 2200
Size: 3319 1210 3098
Fragments arranged by size:
3319 3098 1210
BclI T'GATC_A
Cuts at: 1548 3504 4570 1548
Size: 1956 1066 4605
Fragments arranged by size:
4605 1956 1066
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4784 6238 136
Size: 107 122 71 631 605 3112 1454 1525
Fragments arranged by size:
3112 1525 1454 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7622 7622
Size: 7627
BlpI GC'TnA_GC
Cuts at: 5044 5044
Size: 7627
Bme1580I G_kGCm'C
Cuts at: 1790 2280 3756 4449 5688 6844 1790
Size: 490 1476 693 1239 1156 2573
Fragments arranged by size:
2573 1476 1239 1156 693 490
BmgBI CAC'GTC
Cuts at: 1318 2785 1318
Size: 1467 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 3955 5358
Size: 416 53 83 399 24 883 1655 1403
Cuts at: 5358 6316 442
Size: 958 1753
Fragments arranged by size:
1753 1655 1403 958 883 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2555 2873 3020 3062 3229 4322 4395
Size: 1277 39 318 147 42 167 1093 73
Cuts at: 4395 4689 6287 1239
Size: 294 1598 2579
Fragments arranged by size:
2579 1598 1277 1093 318 294 167 147
73 42 39
Bpu10I CC'TnA_GC
Cuts at: 4110 4353 4110
Size: 243 7384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2445 3242 3308 3496 4624 5752 6617 6915 2445
Size: 797 66 188 1128 1128 865 298 3157
Fragments arranged by size:
3157 1128 1128 865 797 298 188 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2797 6290 882
Size: 617 1298 3493 2219
Fragments arranged by size:
3493 2219 1298 617
BsaAI yAC'GTr
Cuts at: 493 1204 4924 5418 493
Size: 711 3720 494 2702
Fragments arranged by size:
3720 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7621 7621
Size: 7627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3400 5434 5816 6451
Size: 53 83 186 936 1867 2034 382 635
Cuts at: 6451 275
Size: 1451
Fragments arranged by size:
2034 1867 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6060 6458 6732 6879 7290 6060
Size: 398 274 147 411 6397
Fragments arranged by size:
6397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3368 3398 4662 4692 1178
Size: 30 165 30 1965 30 1264 30 4113
Fragments arranged by size:
4113 1965 1264 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2809 2844 3783 4106 4145 4148 4151 4160
Size: 1623 35 939 323 39 3 3 9
Cuts at: 4160 4169 4172 4175 4596 1186
Size: 9 3 3 421 4217
Fragments arranged by size:
4217 1623 939 421 323 39 35 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3367 4337 4358 4727 6830 7326 3367
Size: 970 21 369 2103 496 3668
Fragments arranged by size:
3668 2103 970 496 369 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 3159 3264 3463 4728 1163
Size: 573 705 718 105 199 1265 4062
Fragments arranged by size:
4062 1265 718 705 573 199 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5077 5838 6866 1540
Size: 275 186 3076 761 1028 2301
Fragments arranged by size:
3076 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3663 4173 4401 4496 5688 5773 6844 3663
Size: 510 228 95 1192 85 1071 4446
Fragments arranged by size:
4446 1192 1071 510 228 95 85
BsmI GAATG_Cn'
Cuts at: 5138 5231 5138
Size: 93 7534
BsmBI CGTCTCn'nnnn_
Cuts at: 2492 2492
Size: 7627
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2819 3044 3482 3663 3756 4173
Size: 399 490 539 225 438 181 93 417
Cuts at: 4173 4401 4449 4496 4590 4946 5688 5773 6844
Size: 228 48 47 94 356 742 85 1071
Cuts at: 6844 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 539 490 438 417 399
356 228 225 181 94 93 85 48
47
BspEI T'CCGG_A
Cuts at: 7290 7290
Size: 7627
BspHI T'CATG_A
Cuts at: 5516 7246 5516
Size: 1730 5897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 3708 4855 4982 844
Size: 2698 166 1147 127 3489
Fragments arranged by size:
3489 2698 1147 166 127
BsrBI CCG'CTC
Cuts at: 1394 1823 5514 1394
Size: 429 3691 3507
Fragments arranged by size:
3691 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3360 3918 4662 6122 6296 65
Size: 1183 2112 558 744 1460 174 1396
Fragments arranged by size:
2112 1460 1396 1183 744 558 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3329 6271 6458 1700
Size: 110 1519 2942 187 2869
Fragments arranged by size:
2942 2869 1519 187 110
BsrGI T'GTAC_A
Cuts at: 96 4272 4504 96
Size: 4176 232 3219
Fragments arranged by size:
4176 3219 232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7627
BssSI C'ACGA_G
Cuts at: 5687 6699 5687
Size: 1012 6615
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2679 2935 794
Size: 1885 256 5486
Fragments arranged by size:
5486 1885 256
BstBI TT'CG_AA
Cuts at: 2007 6431 2007
Size: 4424 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7627
BstXI CCAn_nnnn'nTGG
Cuts at: 3425 3425
Size: 7627
BstYI r'GATC_y
Cuts at: 1972 2410 3457 3679 4957 5324 5709 5726 7178
Size: 438 1047 222 1278 367 385 17 1452
Cuts at: 7178 7264 7276 7622 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1278 1047 438 385 367 346
222 86 17 12
BstZ17I GTA'TAC
Cuts at: 3387 3387
Size: 7627
BtgI C'CryG_G
Cuts at: 513 1065 2022 3028 513
Size: 552 957 1006 5112
Fragments arranged by size:
5112 1006 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6888
Fragments arranged by size:
6888 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 4481 4740 5143 5937 5957 2424
Size: 2057 259 403 794 20 4094
Fragments arranged by size:
4094 2057 794 403 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7592
DraI TTT'AAA
Cuts at: 2056 3871 4931 2056
Size: 1815 1060 4752
Fragments arranged by size:
4752 1815 1060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6634 809
Size: 507 5318 1802
Fragments arranged by size:
5318 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2767 3331 5074
Size: 54 1425 50 275 18 937 564 1743
Cuts at: 5074 5963 7592 8
Size: 889 1629 43
Fragments arranged by size:
1743 1629 1425 937 889 564 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5074 1537
Size: 275 3262 4090
Fragments arranged by size:
4090 3262 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2371 3468 3843 4619 5557 1353
Size: 825 193 1097 375 776 938 3423
Fragments arranged by size:
3423 1097 938 825 776 375 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6214 6588 6734 387
Size: 1064 138 4625 374 146 1280
Fragments arranged by size:
4625 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3661 4171 4494 3661
Size: 510 323 6794
Fragments arranged by size:
6794 510 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2516 2555 2604 2813 2873 3020 3062
Size: 393 884 39 49 209 60 147 42
Cuts at: 3062 3164 3229 4086 4322 4335 4395 4545 4689
Size: 102 65 857 236 13 60 150 144
Cuts at: 4689 4782 4859 4868 5690 6287 7074 7342 1239
Size: 93 77 9 822 597 787 268 1524
Fragments arranged by size:
1524 884 857 822 787 597 393 268
236 209 150 147 144 102 93 77
65 60 60 49 42 39 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 2171
Size: 7627
EcoO109I rG'GnC_Cy
Cuts at: 3697 3957 4717 3697
Size: 260 760 6607
Fragments arranged by size:
6607 760 260
EcoRI G'AATT_C
Cuts at: 3019 4936 5314 3019
Size: 1917 378 5332
Fragments arranged by size:
5332 1917 378
EcoRV GAT'ATC
Cuts at: 2113 2113
Size: 7627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 3847 4091 2240
Size: 1607 244 5776
Fragments arranged by size:
5776 1607 244
FseI GG_CCGG'CC
Cuts at: 3333 3333
Size: 7627
FspI TGC'GCA
Cuts at: 6133 6133
Size: 7627
HaeII r_GCGC'y
Cuts at: 1678 2225 6774 1678
Size: 547 4549 2531
Fragments arranged by size:
4549 2531 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3689 3721 4774 4806 6268
Size: 32 135 32 2117 32 1053 32 1462
Cuts at: 6268 6300 6342 6374 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2117 1462 1053 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 4971 5217 669
Size: 988 281 583 2450 246 3079
Fragments arranged by size:
3079 2450 988 583 281 246
HindIII A'AGCT_T
Cuts at: 748 2071 2537 2561 748
Size: 1323 466 24 5814
Fragments arranged by size:
5814 1323 466 24
HpaI GTT'AAC
Cuts at: 2521 5217 2521
Size: 2696 4931
KpnI G_GTAC'C
Cuts at: 4952 4952
Size: 7627
MfeI C'AATT_G
Cuts at: 5226 5226
Size: 7627
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3699 3723 6741 6925 1305
Size: 2394 24 3018 184 2007
Fragments arranged by size:
3018 2394 2007 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2769 10
Size: 54 1425 343 937 4868
Fragments arranged by size:
4868 1425 937 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3767 4512 4650 5585 5944 6103 518
Size: 648 2601 745 138 935 359 159 2042
Fragments arranged by size:
2601 2042 935 745 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2658 4352 4822 5043 5720 6868 7113 1606
Size: 1052 1694 470 221 677 1148 245 2120
Fragments arranged by size:
2120 1694 1148 1052 677 470 245 221
NaeI GCC'GGC
Cuts at: 3331 3331
Size: 7627
NcoI C'CATG_G
Cuts at: 513 1065 2022 3028 513
Size: 552 957 1006 5112
Fragments arranged by size:
5112 1006 957 552
NdeI CA'TA_TG
Cuts at: 387 2764 387
Size: 2377 5250
NgoMIV G'CCGG_C
Cuts at: 3329 3329
Size: 7627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7627
NotI GC'GGCC_GC
Cuts at: 5074 5074
Size: 7627
NspI r_CATG'y
Cuts at: 1132 1229 3790 4985 6530 1132
Size: 97 2561 1195 1545 2229
Fragments arranged by size:
2561 2229 1545 1195 97
PasI CC'CwG_GG
Cuts at: 1348 4258 1348
Size: 2910 4717
PciI A'CATG_T
Cuts at: 6526 6526
Size: 7627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7627
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5696 5728 7209 7241 5696
Size: 32 1481 32 6082
Fragments arranged by size:
6082 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3697 3957 3697
Size: 260 7367
PshAI GACnn'nnGTC
Cuts at: 1613 4036 1613
Size: 2423 5204
PsiI TTA'TAA
Cuts at: 5197 5197
Size: 7627
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2535 2567 2535
Size: 32 7595
PstI C_TGCA'G
Cuts at: 830 2259 2419 4866 4979 830
Size: 1429 160 2447 113 3478
Fragments arranged by size:
3478 2447 1429 160 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 2658 4352 4822 2658
Size: 1694 470 5463
Fragments arranged by size:
5463 1694 470
SacI G_AGCT'C
Cuts at: 3663 4173 4496 3663
Size: 510 323 6794
Fragments arranged by size:
6794 510 323
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SanDI GG'GwC_CC
Cuts at: 3957 3957
Size: 7627
SapI GCTCTTCn'nnn_
Cuts at: 4619 4619
Size: 7627
SbfI CC_TGCA'GG
Cuts at: 4866 4979 4866
Size: 113 7514
ScaI AGT'ACT
Cuts at: 2846 5875 7587 2846
Size: 3029 1712 2886
Fragments arranged by size:
3029 2886 1712
SfcI C'TryA_G
Cuts at: 826 1045 2255 2415 4862 4975 6110 6791 6982
Size: 219 1210 160 2447 113 1135 681 191
Cuts at: 6982 826
Size: 1471
Fragments arranged by size:
2447 1471 1210 1135 681 219 191 160
113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 4954 1872
Size: 3082 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2460 3257 3323 3511 4639
Size: 197 926 282 235 797 66 188 1128
Cuts at: 4639 5731 6632 6894 820
Size: 1092 901 262 1553
Fragments arranged by size:
1553 1128 1092 926 901 797 282 262
235 197 188 66
SnaBI TAC'GTA
Cuts at: 493 4924 5418 493
Size: 4431 494 2702
Fragments arranged by size:
4431 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7627
SphI G_CATG'C
Cuts at: 1132 4985 1132
Size: 3853 3774
SspI AAT'ATT
Cuts at: 5 52 5551 5
Size: 47 5499 2081
Fragments arranged by size:
5499 2081 47
StuI AGG'CCT
Cuts at: 1517 4299 1517
Size: 2782 4845
StyI C'CwwG_G
Cuts at: 513 1065 2022 2583 3028 3372 3996 5066 513
Size: 552 957 561 445 344 624 1070 3074
Fragments arranged by size:
3074 1070 957 624 561 552 445 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5654 5671 5824 6009 6454 325
Size: 1255 4074 17 153 185 445 1498
Fragments arranged by size:
4074 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 4272 4504
Size: 274 80 33 51 1443 867 1428 232
Cuts at: 4504 5873 7585 96
Size: 1369 1712 138
Fragments arranged by size:
1712 1443 1428 1369 867 274 232 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2683 2715 4662 4694 7587 7619 2683
Size: 32 1947 32 2893 32 2691
Fragments arranged by size:
2893 2691 1947 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7627
XbaI T'CTAG_A
Cuts at: 4963 4963
Size: 7627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4255 4255
Size: 7627
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7627
XmaI C'CCGG_G
Cuts at: 1870 4952 1870
Size: 3082 4545
XmnI GAAnn'nnTTC
Cuts at: 3552 4881 5756 3552
Size: 1329 875 5423
Fragments arranged by size:
5423 1329 875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5435 276
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI Bsu36I DraIII FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacII SexAI
SfiI SfoI SrfI SwaI