Restriction map for
pFN21ASDA0048
(Circular) (Six-base) MAPSORT of: pFN21ASDA0048.seq Check: 1532 from: 1 to: 6547
pFN21ASDA0048 6547 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2782 2926 2782
Size: 144 6403
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3620 4357 278
Size: 53 83 186 936 2084 737 2468
Fragments arranged by size:
2468 2084 936 737 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3063 3890 1937
Size: 1126 827 4594
Fragments arranged by size:
4594 1126 827
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 4610 5994 6262 1632
Size: 635 2343 1384 268 1917
Fragments arranged by size:
2343 1917 1384 635 268
AfeI AGC'GCT
Cuts at: 3779 3779
Size: 6547
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 2123 3518 5446 6423 1203
Size: 920 1395 1928 977 1327
Fragments arranged by size:
1928 1395 1327 977 920
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 5134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3225 1184
Size: 2041 4506
AlwNI CAG_nnn'CTG
Cuts at: 2962 3385 4290 5862 2962
Size: 423 905 1572 3647
Fragments arranged by size:
3647 1572 905 423
ApaI G_GGCC'C
Cuts at: 2683 2683
Size: 6547
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 3856 4082 4234 6343 1493
Size: 2363 226 152 2109 1697
Fragments arranged by size:
2363 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3488 3872 1870
Size: 73 1545 384 4545
Fragments arranged by size:
4545 1545 384 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 2911 3501 3868 5323
Size: 325 217 627 696 428 590 367 1455
Cuts at: 5323 618
Size: 1842
Fragments arranged by size:
1842 1455 696 627 590 428 367 325
217
BanII G_rGCy'C
Cuts at: 1391 2683 2870 3437 3717 3866 1391
Size: 1292 187 567 280 149 4072
Fragments arranged by size:
4072 1292 567 280 187 149
BbeI G_GCGC'C
Cuts at: 3505 3505
Size: 6547
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 2358 1548
Size: 810 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3287 5158 136
Size: 107 122 71 631 605 1615 1871 1525
Fragments arranged by size:
1871 1615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 2787 3042 3431 3964 2787
Size: 255 389 533 5370
Fragments arranged by size:
5370 533 389 255
Bme1580I G_kGCm'C
Cuts at: 1790 2683 2916 3634 4608 5764 1790
Size: 893 233 718 974 1156 2573
Fragments arranged by size:
2573 1156 974 893 718 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2043 3277 3760
Size: 416 53 83 399 24 626 1234 483
Cuts at: 3760 4278 5236 442
Size: 518 958 1753
Fragments arranged by size:
1753 1234 958 626 518 483 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2340 3573 5207 1239
Size: 888 213 1233 1634 2579
Fragments arranged by size:
2579 1634 1233 888 213
Bpu10I CC'TnA_GC
Cuts at: 2104 2104
Size: 6547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4672 5537 5835 4672
Size: 865 298 5384
Fragments arranged by size:
5384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 3473 5210 882
Size: 617 1308 666 1737 2219
Fragments arranged by size:
2219 1737 1308 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 3844 4338 493
Size: 711 2315 325 494 2702
Fragments arranged by size:
2702 2315 711 494 325
BsaBI GATnn'nnATC
Cuts at: 2471 6541 2471
Size: 4070 2477
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3617 4354 4736
Size: 53 83 186 936 1969 115 737 382
Cuts at: 4736 5371 275
Size: 635 1451
Fragments arranged by size:
1969 1451 936 737 635 382 186 115
83 53
BsaWI w'CCGG_w
Cuts at: 3080 4980 5378 5652 5799 6210 3080
Size: 1900 398 274 147 411 3417
Fragments arranged by size:
3417 1900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3533 3563 1178
Size: 30 165 30 2130 30 4162
Fragments arranged by size:
4162 2130 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 3802 1186
Size: 1498 1118 3931
Fragments arranged by size:
3931 1498 1118
BseYI C'CCAG_C
Cuts at: 2091 2766 2952 3118 3250 3483 5750 6246 2091
Size: 675 186 166 132 233 2267 496 2392
Fragments arranged by size:
2392 2267 675 496 233 186 166 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 2993 3110 3413 3499 3629 1163
Size: 573 867 390 117 303 86 130 4081
Fragments arranged by size:
4081 867 573 390 303 130 117 86
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3175 3717 4608 4693 5764 3175
Size: 542 891 85 1071 3958
Fragments arranged by size:
3958 1071 891 542 85
BsmI GAATG_Cn'
Cuts at: 3280 4058 4151 3280
Size: 778 93 5676
Fragments arranged by size:
5676 778 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2792 2792
Size: 6547
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2683 2870 2916 3175 3437 3634 3717
Size: 399 893 187 46 259 262 197 83
Cuts at: 3717 3866 4608 4693 5764 1391
Size: 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 742 399 262 259 197
187 149 85 83 46
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 3783 4436 6166 3783
Size: 653 1730 4164
Fragments arranged by size:
4164 1730 653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2224 2782 2859 2890 2926 3902 844
Size: 1223 157 558 77 31 36 976 3489
Fragments arranged by size:
3489 1223 976 558 157 77 36 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2589 3658 4434 1394
Size: 429 766 1069 776 3507
Fragments arranged by size:
3507 1069 776 766 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2632 3312 5042 5216 65
Size: 1183 1384 680 1730 174 1396
Fragments arranged by size:
1730 1396 1384 1183 680 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 2657 4607 5619 2657
Size: 1950 1012 3585
Fragments arranged by size:
3585 1950 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2962 3128 3170 3513 794
Size: 2168 166 42 343 3828
Fragments arranged by size:
3828 2168 343 166 42
BstBI TT'CG_AA
Cuts at: 2007 5351 2007
Size: 3344 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstXI CCAn_nnnn'nTGG
Cuts at: 3185 3185
Size: 6547
BstYI r'GATC_y
Cuts at: 1972 2466 2946 3030 3444 3877 4244 4629 4646
Size: 494 480 84 414 433 367 385 17
Cuts at: 4646 6098 6184 6196 6542 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 494 480 433 414 385 367
346 86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2192 2820 513
Size: 552 957 170 628 4240
Fragments arranged by size:
4240 957 628 552 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5808
Fragments arranged by size:
5808 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 4063 4857 4877 2031
Size: 2032 794 20 3701
Fragments arranged by size:
3701 2032 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DraIII CAC_nnn'GTG
Cuts at: 3707 3707
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3471 3994 4883
Size: 54 1425 50 275 18 1641 523 889
Cuts at: 4883 6512 8
Size: 1629 43
Fragments arranged by size:
1641 1629 1425 889 523 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 3539 3686 4477 1353
Size: 1391 795 147 791 3423
Fragments arranged by size:
3423 1391 795 791 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3715 3715
Size: 6547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2267 2340 3573 4610 5207 5994
Size: 393 495 140 73 1233 1037 597 787
Cuts at: 5994 6262 1239
Size: 268 1524
Fragments arranged by size:
1524 1233 1037 787 597 495 393 268
140 73
EcoNI CCTnn'n_nnAGG
Cuts at: 3104 3104
Size: 6547
EcoO109I rG'GnC_Cy
Cuts at: 2370 2679 2680 2370
Size: 309 1 6237
Fragments arranged by size:
6237 309 1
EcoRI G'AATT_C
Cuts at: 3856 4234 3856
Size: 378 6169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 3637 2266
Size: 1371 5176
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 2310 3505 3781 5694 1678
Size: 632 1195 276 1913 2531
Fragments arranged by size:
2531 1913 1195 632 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2623 2655 3040 3072 3533
Size: 32 135 32 1051 32 385 32 461
Cuts at: 3533 3565 5188 5220 5262 5294 1373
Size: 32 1623 32 42 32 2626
Fragments arranged by size:
2626 1623 1051 461 385 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2610 2650 3891 4137 669
Size: 988 281 672 40 1241 246 3079
Fragments arranged by size:
3079 1241 988 672 281 246 40
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KasI G'GCGC_C
Cuts at: 3501 3501
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3742 5661 5845 1305
Size: 2437 1919 184 2007
Fragments arranged by size:
2437 2007 1919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4725
Fragments arranged by size:
4725 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 3183 4505 4864 5023 518
Size: 648 1413 604 1322 359 159 2042
Fragments arranged by size:
2042 1413 1322 648 604 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2329 2822 2888 3041 3128 3209 3296
Size: 588 135 493 66 153 87 81 87
Cuts at: 3296 3385 3451 3963 4640 5788 6033 1606
Size: 89 66 512 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 588 512 493 245 153
135 89 87 87 81 66 66
NarI GG'CG_CC
Cuts at: 3502 3502
Size: 6547
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5038
Fragments arranged by size:
5038 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 2127 3728 3905 5450 1132
Size: 97 898 1601 177 1545 2229
Fragments arranged by size:
2229 1601 1545 898 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6547
PciI A'CATG_T
Cuts at: 2123 5446 2123
Size: 3323 3224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PfoI T'CCnGG_A
Cuts at: 2317 3105 2317
Size: 788 5759
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PmlI CAC'GTG
Cuts at: 3519 3519
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2370 2370
Size: 6547
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 2679 2679
Size: 6547
PstI C_TGCA'G
Cuts at: 830 2064 3133 3899 830
Size: 1234 1069 766 3478
Fragments arranged by size:
3478 1234 1069 766
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 2329 2888 3041 3128 3209 3296 3385 3451 2329
Size: 559 153 87 81 87 89 66 5425
Fragments arranged by size:
5425 559 153 89 87 87 81 66
SacI G_AGCT'C
Cuts at: 3717 3717
Size: 6547
SacII CC_GC'GG
Cuts at: 2195 2823 2195
Size: 628 5919
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SanDI GG'GwC_CC
Cuts at: 2370 2370
Size: 6547
SapI GCTCTTCn'nnn_
Cuts at: 2744 2744
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SexAI A'CCwGG_T
Cuts at: 3822 3822
Size: 6547
SfcI C'TryA_G
Cuts at: 826 1045 2060 3129 3151 3895 5030 5711 5902
Size: 219 1015 1069 22 744 1135 681 191
Cuts at: 5902 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 1015 744 681 219 191
22
SfoI GGC'GCC
Cuts at: 3503 3503
Size: 6547
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3490 3874 1872
Size: 1618 384 4545
Fragments arranged by size:
4545 1618 384
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4651 5552 5814 820
Size: 197 926 2708 901 262 1553
Fragments arranged by size:
2708 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3844 4338 493
Size: 3351 494 2702
Fragments arranged by size:
3351 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3728 3905 1132
Size: 2596 177 3774
Fragments arranged by size:
3774 2596 177
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2022 2919 3986 513
Size: 552 957 897 1067 3074
Fragments arranged by size:
3074 1067 957 897 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 3455 3749 4574 4591 4744 4929
Size: 1255 516 1359 294 825 17 153 185
Cuts at: 4929 5374 325
Size: 445 1498
Fragments arranged by size:
1498 1359 1255 825 516 445 294 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 4793 6505
Size: 274 80 33 51 1443 466 2350 1712
Cuts at: 6505 96
Size: 138
Fragments arranged by size:
2350 1712 1443 466 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6507 6539 6507
Size: 32 6515
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2477 2477
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3488 3872 1870
Size: 1618 384 4545
Fragments arranged by size:
4545 1618 384
XmnI GAAnn'nnTTC
Cuts at: 3695 3779 4676 3695
Size: 84 897 5566
Fragments arranged by size:
5566 897 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3618 4355 276
Size: 53 83 186 936 2084 737 2468
Fragments arranged by size:
2468 2084 936 737 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SfiI SrfI SwaI