Restriction map for
pFN21ASDA0037
(Circular) (Six-base) MAPSORT of: pFN21ASDA0037.seq Check: 6458 from: 1 to: 7969
pFN21ASDA0037 7969 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 3091 4199 2386
Size: 705 1108 6156
Fragments arranged by size:
6156 1108 705
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5779 278
Size: 53 83 186 936 4243 2468
Fragments arranged by size:
4243 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5312 1937
Size: 3375 4594
Acc65I G'GTAC_C
Cuts at: 3480 4632 5290 3480
Size: 1152 658 6159
Fragments arranged by size:
6159 1152 658
AclI AA'CG_TT
Cuts at: 1563 6096 6469 1563
Size: 4533 373 3063
Fragments arranged by size:
4533 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 2431 3470 4427 4802 6032 7416 7684
Size: 535 264 1039 957 375 1230 1384 268
Cuts at: 7684 1632
Size: 1917
Fragments arranged by size:
1917 1384 1230 1039 957 535 375 268
264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7772
AflIII A'CryG_T
Cuts at: 1203 3081 3201 3216 6868 7845 1203
Size: 1878 120 15 3652 977 1327
Fragments arranged by size:
3652 1878 1327 977 120 15
AgeI A'CCGG_T
Cuts at: 6800 6800
Size: 7969
AhdI GACnn_n'nnGTC
Cuts at: 3205 6698 3205
Size: 3493 4476
AleI CACnn'nnGTG
Cuts at: 1166 3119 4905 1166
Size: 1953 1786 4230
Fragments arranged by size:
4230 1953 1786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7969
AlwNI CAG_nnn'CTG
Cuts at: 2563 2904 4018 4882 5251 5712 7284 2563
Size: 341 1114 864 369 461 1572 3248
Fragments arranged by size:
3248 1572 1114 864 461 369 341
ApaI G_GGCC'C
Cuts at: 2069 3802 5035 2069
Size: 1733 1233 5003
Fragments arranged by size:
5003 1733 1233
ApaLI G'TGCA_C
Cuts at: 3263 6026 7182 3263
Size: 2763 1156 4050
Fragments arranged by size:
4050 2763 1156
ApoI r'AATT_y
Cuts at: 1493 5278 5504 5656 7765 1493
Size: 3785 226 152 2109 1697
Fragments arranged by size:
3785 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6523 160
Size: 872 5491 1606
Fragments arranged by size:
5491 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
AvaI C'yCGr_G
Cuts at: 1870 1943 2637 3151 3485 3795 3955 3972 5153
Size: 73 694 514 334 310 160 17 1181
Cuts at: 5153 5294 1870
Size: 141 4545
Fragments arranged by size:
4545 1181 694 514 334 310 160 141
73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5149 5182 5149
Size: 33 7936
BamHI G'GATC_C
Cuts at: 5299 5666 5299
Size: 367 7602
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 2603 2844 3480 3784
Size: 325 217 627 611 205 241 636 304
Cuts at: 3784 4367 4379 4632 5290 6745 618
Size: 583 12 253 658 1455 1842
Fragments arranged by size:
1842 1455 658 636 627 611 583 325
304 253 241 217 205 12
BanII G_rGCy'C
Cuts at: 1391 2069 2943 3690 3802 4165 4176 4776 4851
Size: 678 874 747 112 363 11 600 75
Cuts at: 4851 5035 5288 1391
Size: 184 253 4072
Fragments arranged by size:
4072 874 747 678 600 363 253 184
112 75 11
BbeI G_GCGC'C
Cuts at: 2607 2607
Size: 7969
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 4219 4415 928
Size: 971 514 1806 196 4482
Fragments arranged by size:
4482 1806 971 514 196
BbvCI CC'TCA_GC
Cuts at: 4022 5207 4022
Size: 1185 6784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 6160 6194 1903
Size: 34 1892 34 2297 34 3678
Fragments arranged by size:
3678 2297 1892 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5861 7071 5861
Size: 1210 6759
BclI T'GATC_A
Cuts at: 1548 5181 1548
Size: 3633 4336
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2474 4280
Size: 107 122 71 631 605 490 312 1806
Cuts at: 4280 4592 6580 136
Size: 312 1988 1525
Fragments arranged by size:
1988 1806 1525 631 605 490 312 312
122 107 71
BglII A'GATC_T
Cuts at: 7964 7964
Size: 7969
BlpI GC'TnA_GC
Cuts at: 3689 4850 5386 3689
Size: 1161 536 6272
Fragments arranged by size:
6272 1161 536
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2403 3267 3324 3802 4869 5035 6030
Size: 279 334 864 57 478 1067 166 995
Cuts at: 6030 7186 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1067 995 864 478 334 279
166 57
BmgBI CAC'GTC
Cuts at: 1318 3202 3217 3559 1318
Size: 1884 15 342 5728
Fragments arranged by size:
5728 1884 342 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 3893 3926
Size: 416 53 83 399 24 1115 1361 33
Cuts at: 3926 5700 6658 442
Size: 1774 958 1753
Fragments arranged by size:
1774 1753 1361 1115 958 416 399 83
53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7969
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 7969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2185 3773 4265 4447 4589 4748 4904 5040
Size: 946 1588 492 182 142 159 156 136
Cuts at: 5040 5118 5129 6629 1239
Size: 78 11 1500 2579
Fragments arranged by size:
2579 1588 1500 946 492 182 159 156
142 136 78 11
Bpu10I CC'TnA_GC
Cuts at: 2300 4022 5207 2300
Size: 1722 1185 5062
Fragments arranged by size:
5062 1722 1185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 3332 6094 6959 7257 2765
Size: 567 2762 865 298 3477
Fragments arranged by size:
3477 2762 865 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3545 4532 5123 6632 882
Size: 617 2046 987 591 1509 2219
Fragments arranged by size:
2219 2046 1509 987 617 591
BsaAI yAC'GTr
Cuts at: 493 1204 4591 4830 5266 5760 493
Size: 711 3387 239 436 494 2702
Fragments arranged by size:
3387 2702 711 494 436 239
BsaBI GATnn'nnATC
Cuts at: 3359 7963 3359
Size: 4604 3365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2604 3461 5776 6158
Size: 53 83 186 936 1071 857 2315 382
Cuts at: 6158 6793 275
Size: 635 1451
Fragments arranged by size:
2315 1451 1071 936 857 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3231 6402 6800 7074 7221 7632 3231
Size: 3171 398 274 147 411 3568
Fragments arranged by size:
3568 3171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4949 4979 1178
Size: 30 165 30 3546 30 4168
Fragments arranged by size:
4168 3546 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 4544 5141 1186
Size: 889 2469 597 4014
Fragments arranged by size:
4014 2469 889 597
BseYI C'CCAG_C
Cuts at: 2341 3410 3591 3928 3967 5251 7172 7668 2341
Size: 1069 181 337 39 1284 1921 496 2642
Fragments arranged by size:
2642 1921 1284 1069 496 337 181 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3970 5119 1163
Size: 573 2234 1149 4013
Fragments arranged by size:
4013 2234 1149 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 3641 5419 6180 7208 1540
Size: 275 186 1258 382 1778 761 1028 2301
Fragments arranged by size:
2301 1778 1258 1028 761 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2943 3267 4296 4527 4776 6030 6115 7186
Size: 435 324 1029 231 249 1254 85 1071
Cuts at: 7186 2508
Size: 3291
Fragments arranged by size:
3291 1254 1071 1029 435 324 249 231
85
BsiWI C'GTAC_G
Cuts at: 3303 3654 3303
Size: 351 7618
BsmI GAATG_Cn'
Cuts at: 5174 5480 5573 5174
Size: 306 93 7570
Fragments arranged by size:
7570 306 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2485 2485
Size: 7969
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2403 2508 2943 3267 3324 3690
Size: 399 279 334 105 435 324 57 366
Cuts at: 3690 3802 4165 4176 4296 4527 4776 4851 4869
Size: 112 363 11 120 231 249 75 18
Cuts at: 4869 5035 5288 6030 6115 7186 1391
Size: 166 253 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 366 363 334
324 279 253 249 231 166 120 112
105 85 75 57 18 11
BspEI T'CCGG_A
Cuts at: 7632 7632
Size: 7969
BspHI T'CATG_A
Cuts at: 5858 7588 5858
Size: 1730 6239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2724 3091 4089 4199 5141 5324 844
Size: 1542 338 367 998 110 942 183 3489
Fragments arranged by size:
3489 1542 998 942 367 338 183 110
BsrBI CCG'CTC
Cuts at: 1394 1823 4888 5856 1394
Size: 429 3065 968 3507
Fragments arranged by size:
3507 3065 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 4129 4755 6464 6638 65
Size: 1183 927 1954 626 1709 174 1396
Fragments arranged by size:
1954 1709 1396 1183 927 626 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6613 6800 1700
Size: 110 4803 187 2869
Fragments arranged by size:
4803 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2430 96
Size: 2334 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7969
BssSI C'ACGA_G
Cuts at: 3697 4526 4868 6029 7041 3697
Size: 829 342 1161 1012 4625
Fragments arranged by size:
4625 1161 1012 829 342
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2623 794
Size: 1829 6140
BstBI TT'CG_AA
Cuts at: 2007 6773 2007
Size: 4766 3203
BstEII G'GTnAC_C
Cuts at: 1184 2792 5119 1184
Size: 1608 2327 4034
Fragments arranged by size:
4034 2327 1608
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 3121 3235 3751 5086 2920
Size: 201 114 516 1335 5803
Fragments arranged by size:
5803 1335 516 201 114
BstYI r'GATC_y
Cuts at: 1972 2673 2994 5299 5666 6051 6068 7520 7606
Size: 701 321 2305 367 385 17 1452 86
Cuts at: 7606 7618 7964 1972
Size: 12 346 1977
Fragments arranged by size:
2305 1977 1452 701 385 367 346 321
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4232 4861 4232
Size: 629 7340
BtgI C'CryG_G
Cuts at: 513 1065 2748 3114 4542 5002 5173 513
Size: 552 1683 366 1428 460 171 3309
Fragments arranged by size:
3309 1683 1428 552 460 366 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4198 4722 4912 487
Size: 683 56 2972 524 190 3544
Fragments arranged by size:
3544 2972 683 524 190 56
BtsI GCAGTG_nn'
Cuts at: 2151 2484 3550 4325 5232 5485 6279 6299 2151
Size: 333 1066 775 907 253 794 20 3821
Fragments arranged by size:
3821 1066 907 794 775 333 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7934
DraI TTT'AAA
Cuts at: 5273 5273
Size: 7969
DraIII CAC_nnn'GTG
Cuts at: 2540 3217 3887 4704 2540
Size: 677 670 817 5805
Fragments arranged by size:
5805 817 677 670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6976 809
Size: 507 5660 1802
Fragments arranged by size:
5660 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2565 2763
Size: 54 1425 50 275 18 315 420 198
Cuts at: 2763 2895 3225 3256 4035 4068 4140 4333 5076
Size: 132 330 31 779 33 72 193 743
Cuts at: 5076 5416 6305 7934 8
Size: 340 889 1629 43
Fragments arranged by size:
1629 1425 889 779 743 420 340 330
315 275 198 193 132 72 54 50
43 33 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 5416 1537
Size: 275 1444 2160 4090
Fragments arranged by size:
4090 2160 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 4301 5899 1353
Size: 1298 1650 1598 3423
Fragments arranged by size:
3423 1650 1598 1298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2666 3586 4970 6556 6930 7076
Size: 1064 138 1077 920 1384 1586 374 146
Cuts at: 7076 387
Size: 1280
Fragments arranged by size:
1586 1384 1280 1077 1064 920 374 146
138
EcoICRI GAG'CTC
Cuts at: 2941 4774 2941
Size: 1833 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2185 2431 3470 3773 4265 4427
Size: 393 535 18 246 1039 303 492 162
Cuts at: 4427 4447 4589 4748 4802 4904 5040 5118 5129
Size: 20 142 159 54 102 136 78 11
Cuts at: 5129 6032 6629 7416 7684 1239
Size: 903 597 787 268 1524
Fragments arranged by size:
1524 1039 903 787 597 535 492 393
303 268 246 162 159 142 136 102
78 54 20 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 3296 3755 2051
Size: 1245 459 6265
Fragments arranged by size:
6265 1245 459
EcoO109I rG'GnC_Cy
Cuts at: 2065 2066 2194 2304 2641 3337 3702 4402 5031
Size: 1 128 110 337 696 365 700 629
Cuts at: 5031 5149 2065
Size: 118 4885
Fragments arranged by size:
4885 700 696 629 365 337 128 118
110 1
EcoRI G'AATT_C
Cuts at: 5278 5656 5278
Size: 378 7591
FspI TGC'GCA
Cuts at: 2690 3185 3470 6475 2690
Size: 495 285 3005 4184
Fragments arranged by size:
4184 3005 495 285
FspAI rTGC'GCAy
Cuts at: 3470 3470
Size: 7969
HaeII r_GCGC'y
Cuts at: 1678 2041 2296 2315 2342 2463 2607 2704 3598
Size: 363 255 19 27 121 144 97 894
Cuts at: 3598 7116 1678
Size: 3518 2531
Fragments arranged by size:
3518 2531 894 363 255 144 121 97
27 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3191 3223 3656 3688 6610
Size: 32 135 32 1619 32 433 32 2922
Cuts at: 6610 6642 6684 6716 1373
Size: 32 42 32 2626
Fragments arranged by size:
2922 2626 1619 433 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4714 5313 5559 669
Size: 988 281 2776 599 246 3079
Fragments arranged by size:
3079 2776 988 599 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7969
HpaI GTT'AAC
Cuts at: 5559 5559
Size: 7969
KasI G'GCGC_C
Cuts at: 2603 2603
Size: 7969
KpnI G_GTAC'C
Cuts at: 3484 4636 5294 3484
Size: 1152 658 6159
Fragments arranged by size:
6159 1152 658
MfeI C'AATT_G
Cuts at: 5568 5568
Size: 7969
MluI A'CGCG_T
Cuts at: 7845 7845
Size: 7969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3621 7083 7267 1305
Size: 2316 3462 184 2007
Fragments arranged by size:
3462 2316 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 2765 2897 3227 4070
Size: 54 1425 343 735 198 132 330 843
Cuts at: 4070 5078 10
Size: 1008 2901
Fragments arranged by size:
2901 1425 1008 843 735 343 330 198
132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 2753 2850 3119 3131 3137 4832
Size: 648 1581 6 97 269 12 6 1695
Cuts at: 4832 4905 5927 6286 6445 518
Size: 73 1022 359 159 2042
Fragments arranged by size:
2042 1695 1581 1022 648 359 269 159
97 73 12 6 6
MspA1I CmG'CkG
Cuts at: 1606 2122 2560 2893 3334 4197 4797 4841 5385
Size: 516 438 333 441 863 600 44 544
Cuts at: 5385 6062 7210 7455 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 600 544 516 441
438 333 245 44
NarI GG'CG_CC
Cuts at: 2604 2604
Size: 7969
NcoI C'CATG_G
Cuts at: 513 1065 2748 4542 5002 513
Size: 552 1683 1794 460 3480
Fragments arranged by size:
3480 1794 1683 552 460
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7969
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7969
NotI GC'GGCC_GC
Cuts at: 5416 5416
Size: 7969
NspI r_CATG'y
Cuts at: 1132 1229 2455 2809 2986 3085 3136 3625 4898
Size: 97 1226 354 177 99 51 489 1273
Cuts at: 4898 5327 6872 1132
Size: 429 1545 2229
Fragments arranged by size:
2229 1545 1273 1226 489 429 354 177
99 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7969
PciI A'CATG_T
Cuts at: 3081 6868 3081
Size: 3787 4182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2500 4342 4882 1108
Size: 1392 1842 540 4195
Fragments arranged by size:
4195 1842 1392 540
PfoI T'CCnGG_A
Cuts at: 3750 4394 3750
Size: 644 7325
PmeI GTTT'AAAC
Cuts at: 5273 5273
Size: 7969
PmlI CAC'GTG
Cuts at: 4591 4591
Size: 7969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6038 6070 7551 7583 6038
Size: 32 1481 32 6424
Fragments arranged by size:
6424 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2641 3337 4402 2194
Size: 447 696 1065 5761
Fragments arranged by size:
5761 1065 696 447
PshAI GACnn'nnGTC
Cuts at: 1613 3557 1613
Size: 1944 6025
PsiI TTA'TAA
Cuts at: 5539 5539
Size: 7969
PspOMI G'GGCC_C
Cuts at: 2065 3798 5031 2065
Size: 1733 1233 5003
Fragments arranged by size:
5003 1733 1233
PspXI vC'TCGA_Gb
Cuts at: 3485 3485
Size: 7969
PstI C_TGCA'G
Cuts at: 830 2559 4196 4846 5321 830
Size: 1729 1637 650 475 3478
Fragments arranged by size:
3478 1729 1637 650 475
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7969
PvuII CAG'CTG
Cuts at: 2560 2893 4197 4841 2560
Size: 333 1304 644 5688
Fragments arranged by size:
5688 1304 644 333
SacI G_AGCT'C
Cuts at: 2943 4776 2943
Size: 1833 6136
SalI G'TCGA_C
Cuts at: 1936 5311 1936
Size: 3375 4594
SanDI GG'GwC_CC
Cuts at: 2194 3337 2194
Size: 1143 6826
SapI GCTCTTCn'nnn_
Cuts at: 4301 4301
Size: 7969
SbfI CC_TGCA'GG
Cuts at: 5321 5321
Size: 7969
ScaI AGT'ACT
Cuts at: 6217 7929 6217
Size: 1712 6257
SexAI A'CCwGG_T
Cuts at: 3372 4287 4428 3372
Size: 915 141 6913
Fragments arranged by size:
6913 915 141
SfcI C'TryA_G
Cuts at: 826 1045 2555 4192 4842 5317 6452 7133 7324
Size: 219 1510 1637 650 475 1135 681 191
Cuts at: 7324 826
Size: 1471
Fragments arranged by size:
1637 1510 1471 1135 681 650 475 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4280 4280
Size: 7969
SfoI GGC'GCC
Cuts at: 2605 2605
Size: 7969
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7969
SmaI CCC'GGG
Cuts at: 1872 2639 3797 5296 1872
Size: 767 1158 1499 4545
Fragments arranged by size:
4545 1499 1158 767
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2780 3347 3485 3955 6073 6974
Size: 197 926 837 567 138 470 2118 901
Cuts at: 6974 7236 820
Size: 262 1553
Fragments arranged by size:
2118 1553 926 901 837 567 470 262
197 138
SnaBI TAC'GTA
Cuts at: 493 5266 5760 493
Size: 4773 494 2702
Fragments arranged by size:
4773 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7969
SphI G_CATG'C
Cuts at: 1132 2455 2809 2986 4898 5327 1132
Size: 1323 354 177 1912 429 3774
Fragments arranged by size:
3774 1912 1323 429 354 177
SspI AAT'ATT
Cuts at: 5 52 5893 5
Size: 47 5841 2081
Fragments arranged by size:
5841 2081 47
StuI AGG'CCT
Cuts at: 1517 3577 4134 4341 1517
Size: 2060 557 207 5145
Fragments arranged by size:
5145 2060 557 207
StyI C'CwwG_G
Cuts at: 513 1065 2031 2307 2748 2913 2967 3432 3630
Size: 552 966 276 441 165 54 465 198
Cuts at: 3630 4336 4542 5002 5408 513
Size: 706 206 460 406 3074
Fragments arranged by size:
3074 966 706 552 465 460 441 406
276 206 198 165 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 3802 3875 4213 5996 6013 6166
Size: 1255 971 1251 73 338 1783 17 153
Cuts at: 6166 6351 6796 325
Size: 185 445 1498
Fragments arranged by size:
1783 1498 1255 1251 971 445 338 185
153 73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 3913 6215
Size: 274 80 33 51 1443 453 1483 2302
Cuts at: 6215 7927 96
Size: 1712 138
Fragments arranged by size:
2302 1712 1483 1443 453 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2526 2558 3203 3235 7929 7961 2526
Size: 32 645 32 4694 32 2534
Fragments arranged by size:
4694 2534 645 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4652 1316
Size: 3336 4633
XbaI T'CTAG_A
Cuts at: 5305 5305
Size: 7969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 2221 2545 2920 4317 4426 2158
Size: 63 324 375 1397 109 5701
Fragments arranged by size:
5701 1397 375 324 109 63
XhoI C'TCGA_G
Cuts at: 1943 3485 3955 1943
Size: 1542 470 5957
Fragments arranged by size:
5957 1542 470
XmaI C'CCGG_G
Cuts at: 1870 2637 3795 5294 1870
Size: 767 1158 1499 4545
Fragments arranged by size:
4545 1499 1158 767
XmnI GAAnn'nnTTC
Cuts at: 4511 6098 4511
Size: 1587 6382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5777 276
Size: 53 83 186 936 4243 2468
Fragments arranged by size:
4243 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BstZ17I EcoRV FalI
FseI NaeI NgoMIV NruI NsiI PacI PsrI RsrII
SacII SrfI SwaI