Restriction map for
pFN21ASDA0033
(Circular) (Six-base) MAPSORT of: pFN21ASDA0033.seq Check: 3125 from: 1 to: 5602
pFN21ASDA0033 5602 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2431 2547 2945 1937
Size: 494 116 398 4594
Fragments arranged by size:
4594 494 398 116
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 2106 4331 2106
Size: 2225 3377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 1184
Size: 1234 4368
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2242 2888 2911 3137 3289 5398 1493
Size: 749 646 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 749 646 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 2667 4156 160
Size: 872 1635 1489 1606
Fragments arranged by size:
1635 1606 1489 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2498 2923 4378 618
Size: 325 217 627 329 382 425 1455 1842
Fragments arranged by size:
1842 1455 627 425 382 329 325 217
BanII G_rGCy'C
Cuts at: 1391 2921 1391
Size: 1530 4072
BbeI G_GCGC'C
Cuts at: 2120 2120
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BbvCI CC'TCA_GC
Cuts at: 2250 2250
Size: 5602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2759 4213 136
Size: 107 122 71 631 605 1087 1454 1525
Fragments arranged by size:
1525 1454 1087 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2627
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4262 1239
Size: 3023 2579
Bpu10I CC'TnA_GC
Cuts at: 2250 2250
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 2124 2530 4265 882
Size: 617 615 10 406 1735 2219
Fragments arranged by size:
2219 1735 617 615 406 10
BsaAI yAC'GTr
Cuts at: 493 1204 2899 3393 493
Size: 711 1695 494 2702
Fragments arranged by size:
2702 1695 711 494
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2117 3409 3791 4426
Size: 53 83 186 936 584 1292 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1292 936 635 584 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2787 2817 1178
Size: 30 165 30 1384 30 3963
Fragments arranged by size:
3963 1384 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2051 3052 3813 4841 1540
Size: 275 186 50 1001 761 1028 2301
Fragments arranged by size:
2301 1028 1001 761 275 186 50
BsiHKAI G_wGCw'C
Cuts at: 3663 3748 4819 3663
Size: 85 1071 4446
Fragments arranged by size:
4446 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2921 3663 3748 4819 1391
Size: 399 1131 742 85 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2578 2957 844
Size: 1734 379 3489
Fragments arranged by size:
3489 1734 379
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 2007 4406 2007
Size: 2399 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597
Size: 960 367 385 17 1452 86 12 346
Cuts at: 5597 1972
Size: 1977
Fragments arranged by size:
1977 1452 960 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2432 2548 2432
Size: 116 5486
BtgI C'CryG_G
Cuts at: 513 1065 2022 2079 513
Size: 552 957 57 4036
Fragments arranged by size:
4036 957 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567
Size: 54 1425 50 275 18 1219 889 1629
Cuts at: 5567 8
Size: 43
Fragments arranged by size:
1629 1425 1219 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3665 4262 5049 5317 1239
Size: 393 2033 597 787 268 1524
Fragments arranged by size:
2033 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 5602
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2064 2120 4749 1678
Size: 386 56 2629 2531
Fragments arranged by size:
2629 2531 386 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 4243 4275 4317
Size: 32 135 32 520 32 2119 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 2119 520 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2116 2116
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 3018 3695 4843 5088 1606
Size: 490 922 677 1148 245 2120
Fragments arranged by size:
2120 1148 922 677 490 245
NarI GG'CG_CC
Cuts at: 2117 2117
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4093
Fragments arranged by size:
4093 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2807 2807
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2215 2954 830
Size: 1385 739 3478
Fragments arranged by size:
3478 1385 739
PvuI CG_AT'CG
Cuts at: 2001 2051 2001
Size: 50 5552
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 2361 3850 5562 2361
Size: 1489 1712 2401
Fragments arranged by size:
2401 1712 1489
SfcI C'TryA_G
Cuts at: 826 1045 2211 2950 4085 4766 4957 826
Size: 219 1166 739 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 739 681 219 191
SfoI GGC'GCC
Cuts at: 2118 2118
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2899 3393 493
Size: 2406 494 2702
Fragments arranged by size:
2702 2406 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 2820 3526 5
Size: 47 2768 706 2081
Fragments arranged by size:
2768 2081 706 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2022 2307 3041 513
Size: 552 957 285 734 3074
Fragments arranged by size:
3074 957 734 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2359 3848 5560
Size: 274 80 33 51 1443 382 1489 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1489 1443 382 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI