Restriction map for
pFN21ASDA0032
(Circular) (Six-base) MAPSORT of: pFN21ASDA0032.seq Check: 3553 from: 1 to: 7879
pFN21ASDA0032 7879 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4036 4036
Size: 7879
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5689 278
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5222 1937
Size: 3285 4594
Acc65I G'GTAC_C
Cuts at: 5200 5200
Size: 7879
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3175 4542 4788 5942 7326 7594 1632
Size: 1543 1367 246 1154 1384 268 1917
Fragments arranged by size:
1917 1543 1384 1367 1154 268 246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7682
AflIII A'CryG_T
Cuts at: 1203 2192 4359 4361 6778 7755 1203
Size: 989 2167 2 2417 977 1327
Fragments arranged by size:
2417 2167 1327 989 977 2
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 6608 6608
Size: 7879
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 1184
Size: 906 6973
AlwNI CAG_nnn'CTG
Cuts at: 2651 4031 5622 7194 2651
Size: 1380 1591 1572 3336
Fragments arranged by size:
3336 1591 1572 1380
ApaLI G'TGCA_C
Cuts at: 5936 7092 5936
Size: 1156 6723
ApoI r'AATT_y
Cuts at: 1493 4268 4685 5188 5414 5566 7675 1493
Size: 2775 417 503 226 152 2109 1697
Fragments arranged by size:
2775 2109 1697 503 417 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 3084 4556 5204 1870
Size: 73 1141 1472 648 4545
Fragments arranged by size:
4545 1472 1141 648 73
BamHI G'GATC_C
Cuts at: 2589 5209 5576 2589
Size: 2620 367 4892
Fragments arranged by size:
4892 2620 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5200 6655 618
Size: 325 217 627 3413 1455 1842
Fragments arranged by size:
3413 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 2263 2623 2694 4794 5198 1391
Size: 706 166 360 71 2100 404 4072
Fragments arranged by size:
4072 2100 706 404 360 166 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3903 4072 4503 4705 928
Size: 971 2004 169 431 202 4102
Fragments arranged by size:
4102 2004 971 431 202 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6070 6104 1903
Size: 34 4133 34 3678
Fragments arranged by size:
4133 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3625 3952 5771 6981 3625
Size: 327 1819 1210 4523
Fragments arranged by size:
4523 1819 1210 327
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7879
BfrBI ATG'CAT
Cuts at: 2975 2975
Size: 7879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2618 6490 136
Size: 107 122 71 631 605 946 3872 1525
Fragments arranged by size:
3872 1525 946 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3120 7874 3120
Size: 4754 3125
BlpI GC'TnA_GC
Cuts at: 2091 4178 5296 2091
Size: 2087 1118 4674
Fragments arranged by size:
4674 2087 1118
Bme1580I G_kGCm'C
Cuts at: 1790 2406 3015 3035 5128 5940 7096 1790
Size: 616 609 20 2093 812 1156 2573
Fragments arranged by size:
2573 2093 1156 812 616 609 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3018 3064
Size: 416 53 83 399 24 811 790 46
Cuts at: 3064 3493 4855 5610 6568 442
Size: 429 1362 755 958 1753
Fragments arranged by size:
1753 1362 958 811 790 755 429 416
399 83 53 46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 7879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 3150 3554 4118 6539 1239
Size: 1364 547 404 564 2421 2579
Fragments arranged by size:
2579 2421 1364 564 547 404
Bpu10I CC'TnA_GC
Cuts at: 2533 2601 2533
Size: 68 7811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4808 6004 6869 7167 4808
Size: 1196 865 298 5520
Fragments arranged by size:
5520 1196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 3129 3672 6542 882
Size: 617 550 1080 543 2870 2219
Fragments arranged by size:
2870 2219 1080 617 550 543
BsaAI yAC'GTr
Cuts at: 493 1204 4362 5176 5670 493
Size: 711 3158 814 494 2702
Fragments arranged by size:
3158 2702 814 711 494
BsaBI GATnn'nnATC
Cuts at: 4338 4994 5024 7873 4338
Size: 656 30 2849 4344
Fragments arranged by size:
4344 2849 656 30
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5686 6068 6703 275
Size: 53 83 186 936 4153 382 635 1451
Fragments arranged by size:
4153 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6312 6710 6984 7131 7542 6312
Size: 398 274 147 411 6649
Fragments arranged by size:
6649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2347 2377 4641 4671 1178
Size: 30 165 30 944 30 2264 30 4386
Fragments arranged by size:
4386 2264 944 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3147 4586 5063 1186
Size: 1961 1439 477 4002
Fragments arranged by size:
4002 1961 1439 477
BseYI C'CCAG_C
Cuts at: 2661 3641 7082 7578 2661
Size: 980 3441 496 2962
Fragments arranged by size:
3441 2962 980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2370 3738 1163
Size: 573 634 1368 5304
Fragments arranged by size:
5304 1368 634 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4987 5329 6090 7118 1540
Size: 275 186 2986 342 761 1028 2301
Fragments arranged by size:
2986 2301 1028 761 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 4377 4794 5940 6025 7096 2263
Size: 2114 417 1146 85 1071 3046
Fragments arranged by size:
3046 2114 1146 1071 417 85
BsmI GAATG_Cn'
Cuts at: 4182 5390 5483 4182
Size: 1208 93 6578
Fragments arranged by size:
6578 1208 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2263 2406 2623 2694 3015 3035
Size: 399 307 166 143 217 71 321 20
Cuts at: 3035 4377 4794 5128 5198 5940 6025 7096 1391
Size: 1342 417 334 70 742 85 1071 2174
Fragments arranged by size:
2174 1342 1071 742 417 399 334 321
307 217 166 143 85 71 70 20
BspEI T'CCGG_A
Cuts at: 7542 7542
Size: 7879
BspHI T'CATG_A
Cuts at: 2376 3690 4729 4930 5768 7498 2376
Size: 1314 1039 201 838 1730 2757
Fragments arranged by size:
2757 1730 1314 1039 838 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 4036 4077 5234 844
Size: 1238 1954 41 1157 3489
Fragments arranged by size:
3489 1954 1238 1157 41
BsrBI CCG'CTC
Cuts at: 1394 1823 5766 1394
Size: 429 3943 3507
Fragments arranged by size:
3943 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2464 6374 6548 65
Size: 1183 1216 3910 174 1396
Fragments arranged by size:
3910 1396 1216 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4250 5059 6523 6710 1700
Size: 110 2440 809 1464 187 2869
Fragments arranged by size:
2869 2440 1464 809 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7879
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7879
BssSI C'ACGA_G
Cuts at: 3881 5939 6951 3881
Size: 2058 1012 4809
Fragments arranged by size:
4809 2058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4247 794
Size: 3453 4426
BstBI TT'CG_AA
Cuts at: 2007 6683 2007
Size: 4676 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7879
BstXI CCAn_nnnn'nTGG
Cuts at: 2105 3883 2105
Size: 1778 6101
BstYI r'GATC_y
Cuts at: 1972 2589 3120 5209 5576 5961 5978 7430 7516
Size: 617 531 2089 367 385 17 1452 86
Cuts at: 7516 7528 7874 1972
Size: 12 346 1977
Fragments arranged by size:
2089 1977 1452 617 531 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2518 513
Size: 552 1453 5874
Fragments arranged by size:
5874 1453 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 2406 4248 5395 6189 6209 2406
Size: 1842 1147 794 20 4076
Fragments arranged by size:
4076 1842 1147 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7844
DraI TTT'AAA
Cuts at: 3100 3370 5183 3100
Size: 270 1813 5796
Fragments arranged by size:
5796 1813 270
DraIII CAC_nnn'GTG
Cuts at: 2161 2161
Size: 7879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 6886 809
Size: 507 810 4760 1802
Fragments arranged by size:
4760 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2488 3070 3615
Size: 54 1425 50 275 18 658 582 545
Cuts at: 3615 4110 4890 5007 5326 6215 7844 8
Size: 495 780 117 319 889 1629 43
Fragments arranged by size:
1629 1425 889 780 658 582 545 495
319 275 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5326 1537
Size: 275 3514 4090
Fragments arranged by size:
4090 3514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2875 4799 4854 5809 1353
Size: 1522 1924 55 955 3423
Fragments arranged by size:
3423 1924 1522 955 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6466 6840 6986 387
Size: 1064 138 4877 374 146 1280
Fragments arranged by size:
4877 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2261 4792 2261
Size: 2531 5348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 3150 3175 3554 4118 4542 4788
Size: 393 971 547 25 379 564 424 246
Cuts at: 4788 5942 6539 7326 7594 1239
Size: 1154 597 787 268 1524
Fragments arranged by size:
1524 1154 971 787 597 564 547 424
393 379 268 246 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3569 3569
Size: 7879
EcoO109I rG'GnC_Cy
Cuts at: 2577 5140 2577
Size: 2563 5316
EcoRI G'AATT_C
Cuts at: 5188 5566 5188
Size: 378 7501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 3769 4507 4981 3139
Size: 630 738 474 6037
Fragments arranged by size:
6037 738 630 474
FspI TGC'GCA
Cuts at: 6385 6385
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 2826 7026 1678
Size: 1148 4200 2531
Fragments arranged by size:
4200 2531 1148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 2699 2731 3246
Size: 32 135 32 775 32 320 32 515
Cuts at: 3246 3278 3389 3421 3862 3894 4401 4433 6520
Size: 32 111 32 441 32 507 32 2087
Cuts at: 6520 6552 6594 6626 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2087 775 515 507 441 320 135
111 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3187 5223 5469 669
Size: 988 281 934 315 2036 246 3079
Fragments arranged by size:
3079 2036 988 934 315 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7879
HpaI GTT'AAC
Cuts at: 2872 5469 2872
Size: 2597 5282
KpnI G_GTAC'C
Cuts at: 5204 5204
Size: 7879
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 7755 7755
Size: 7879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3308 4138 6993 7177 1305
Size: 2003 830 2855 184 2007
Fragments arranged by size:
2855 2007 2003 830 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 3072 3617 4112 5009
Size: 54 1425 343 658 582 545 495 897
Cuts at: 5009 10
Size: 2880
Fragments arranged by size:
2880 1425 897 658 582 545 495 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3276 5837 6196 6355 518
Size: 648 1119 991 2561 359 159 2042
Fragments arranged by size:
2561 2042 1119 991 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2335 5043 5295 5972 7120 7365 1606
Size: 729 2708 252 677 1148 245 2120
Fragments arranged by size:
2708 2120 1148 729 677 252 245
NcoI C'CATG_G
Cuts at: 513 1065 2518 513
Size: 552 1453 5874
Fragments arranged by size:
5874 1453 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NsiI A_TGCA'T
Cuts at: 2977 2977
Size: 7879
NspI r_CATG'y
Cuts at: 1132 1229 2196 2295 3842 5237 6782 1132
Size: 97 967 99 1547 1395 1545 2229
Fragments arranged by size:
2229 1547 1545 1395 967 99 97
PasI CC'CwG_GG
Cuts at: 1348 3389 1348
Size: 2041 5838
PciI A'CATG_T
Cuts at: 2192 6778 2192
Size: 4586 3293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 6676
PfoI T'CCnGG_A
Cuts at: 3570 3570
Size: 7879
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PmlI CAC'GTG
Cuts at: 4362 4362
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 7461 7493 5948
Size: 32 1481 32 6334
Fragments arranged by size:
6334 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2577 2577
Size: 7879
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7879
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4823 4855 4823
Size: 32 7847
PstI C_TGCA'G
Cuts at: 830 2079 3322 4074 5019 5231 830
Size: 1249 1243 752 945 212 3478
Fragments arranged by size:
3478 1249 1243 945 752 212
PvuI CG_AT'CG
Cuts at: 2001 4987 2001
Size: 2986 4893
PvuII CAG'CTG
Cuts at: 2335 2335
Size: 7879
SacI G_AGCT'C
Cuts at: 2263 4794 2263
Size: 2531 5348
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SapI GCTCTTCn'nnn_
Cuts at: 4799 4799
Size: 7879
SbfI CC_TGCA'GG
Cuts at: 2079 5231 2079
Size: 3152 4727
ScaI AGT'ACT
Cuts at: 3651 4785 5054 6127 7839 3651
Size: 1134 269 1073 1712 3691
Fragments arranged by size:
3691 1712 1134 1073 269
SexAI A'CCwGG_T
Cuts at: 2056 2056
Size: 7879
SfcI C'TryA_G
Cuts at: 826 1045 2075 3318 4037 4070 5015 5227 6362
Size: 219 1030 1243 719 33 945 212 1135
Cuts at: 6362 7043 7234 826
Size: 681 191 1471
Fragments arranged by size:
1471 1243 1135 1030 945 719 681 219
212 191 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7879
SmaI CCC'GGG
Cuts at: 1872 4558 5206 1872
Size: 2686 648 4545
Fragments arranged by size:
4545 2686 648
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4787 5983 6884 7146 820
Size: 197 926 2844 1196 901 262 1553
Fragments arranged by size:
2844 1553 1196 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5176 5670 493
Size: 4683 494 2702
Fragments arranged by size:
4683 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SspI AAT'ATT
Cuts at: 5 52 2439 3686 5803 5
Size: 47 2387 1247 2117 2081
Fragments arranged by size:
2387 2117 2081 1247 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7879
StyI C'CwwG_G
Cuts at: 513 1065 2208 2518 3606 5318 513
Size: 552 1143 310 1088 1712 3074
Fragments arranged by size:
3074 1712 1143 1088 552 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2340 4531 5906 5923 6076 6261 6706
Size: 1255 760 2191 1375 17 153 185 445
Cuts at: 6706 325
Size: 1498
Fragments arranged by size:
2191 1498 1375 1255 760 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3148 3649 4783
Size: 274 80 33 51 1443 1171 501 1134
Cuts at: 4783 5052 5100 6125 7837 96
Size: 269 48 1025 1712 138
Fragments arranged by size:
1712 1443 1171 1134 1025 501 274 269
138 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7839 7871 7839
Size: 32 7847
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7879
XbaI T'CTAG_A
Cuts at: 2887 3457 5215 2887
Size: 570 1758 5551
Fragments arranged by size:
5551 1758 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2525 3625 5002 2215
Size: 310 1100 1377 5092
Fragments arranged by size:
5092 1377 1100 310
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7879
XmaI C'CCGG_G
Cuts at: 1870 4556 5204 1870
Size: 2686 648 4545
Fragments arranged by size:
4545 2686 648
XmnI GAAnn'nnTTC
Cuts at: 2543 3686 4079 4272 4926 6008 2543
Size: 1143 393 193 654 1082 4414
Fragments arranged by size:
4414 1143 1082 654 393 193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5687 276
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII
SacII SanDI SfiI SfoI SrfI SwaI