Restriction map for
pFN21ASDA0028
(Circular) (Six-base) MAPSORT of: pFN21ASDA0028.seq Check: 2659 from: 1 to: 7438
pFN21ASDA0028 7438 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2668 2668
Size: 7438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2174 2334 4781 1937
Size: 237 160 2447 4594
Fragments arranged by size:
4594 2447 237 160
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2952 3023 3228 3501 3598 5501 6885 7153
Size: 1320 71 205 273 97 1903 1384 268
Cuts at: 7153 1632
Size: 1917
Fragments arranged by size:
1917 1903 1384 1320 273 268 205 97
71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 2549 2784 6337 7314 1203
Size: 1346 235 3553 977 1327
Fragments arranged by size:
3553 1346 1327 977 235
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 3434 3659 1166
Size: 2268 225 4945
Fragments arranged by size:
4945 2268 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 7406
AlwNI CAG_nnn'CTG
Cuts at: 2252 4171 5181 6753 2252
Size: 1919 1010 1572 2937
Fragments arranged by size:
2937 1919 1572 1010
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2241 2270 3147 4270 4578 4625 4747 4973
Size: 748 29 877 1123 308 47 122 226
Cuts at: 4973 5125 7234 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1123 877 748 308 226 152
122 47 29
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 3312 4346 4602 4696 4763 1870
Size: 73 1369 1034 256 94 67 4545
Fragments arranged by size:
4545 1369 1034 256 94 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2714 2747 3576 3609 2714
Size: 33 829 33 6543
Fragments arranged by size:
6543 829 33 33
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2945 4438 4759 6214 618
Size: 325 217 627 1158 1493 321 1455 1842
Fragments arranged by size:
1842 1493 1455 1158 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 4757 1391
Size: 3366 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6467
BbvCI CC'TCA_GC
Cuts at: 4164 4191 4164
Size: 27 7411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3577 5330 6540 3577
Size: 1753 1210 4475
Fragments arranged by size:
4475 1753 1210
BclI T'GATC_A
Cuts at: 1548 3760 4554 1548
Size: 2212 794 4432
Fragments arranged by size:
4432 2212 794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2921 6049 136
Size: 107 122 71 631 605 1249 3128 1525
Fragments arranged by size:
3128 1525 1249 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4416 7433 4416
Size: 3017 4421
BlpI GC'TnA_GC
Cuts at: 4855 4855
Size: 7438
Bme1580I G_kGCm'C
Cuts at: 1790 2994 3392 3441 4372 4459 4590 5499 6655
Size: 1204 398 49 931 87 131 909 1156
Cuts at: 6655 1790
Size: 2573
Fragments arranged by size:
2573 1204 1156 931 909 398 131 87
49
BmgBI CAC'GTC
Cuts at: 1318 3337 3930 1318
Size: 2019 593 4826
Fragments arranged by size:
4826 2019 593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2642 2650 3147
Size: 416 53 83 399 24 1225 8 497
Cuts at: 3147 3394 3410 5169 6127 442
Size: 247 16 1759 958 1753
Fragments arranged by size:
1759 1753 1225 958 497 416 399 247
83 53 24 16 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4279 4279
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2730 3464 4472 6098 1239
Size: 1491 734 1008 1626 2579
Fragments arranged by size:
2579 1626 1491 1008 734
Bpu10I CC'TnA_GC
Cuts at: 4164 4191 4164
Size: 27 7411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 5563 6428 6726 3034
Size: 2529 865 298 3746
Fragments arranged by size:
3746 2529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6101 882
Size: 617 4602 2219
Fragments arranged by size:
4602 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 2550 2956 4735 5229 493
Size: 711 1074 272 406 1779 494 2702
Fragments arranged by size:
2702 1779 1074 711 494 406 272
BsaBI GATnn'nnATC
Cuts at: 7432 7432
Size: 7438
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5245 5627 6262 275
Size: 53 83 186 936 3712 382 635 1451
Fragments arranged by size:
3712 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2703 2733 4205 4235 1178
Size: 30 165 30 1300 30 1472 30 4381
Fragments arranged by size:
4381 1472 1300 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7438
BseYI C'CCAG_C
Cuts at: 2529 2606 2969 3078 4158 4645 6641 7137 2529
Size: 77 363 109 1080 487 1996 496 2830
Fragments arranged by size:
2830 1996 1080 496 487 363 109 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 1163
Size: 573 561 6304
Fragments arranged by size:
6304 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5499 5584 6655 5499
Size: 85 1071 6282
Fragments arranged by size:
6282 1071 85
BsmI GAATG_Cn'
Cuts at: 4949 5042 4949
Size: 93 7345
BsmBI CGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 7438
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2994 3392 3441 4372 4459 4590 4757
Size: 399 1204 398 49 931 87 131 167
Cuts at: 4757 5499 5584 6655 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1204 1071 931 742 399 398 167
131 87 85 49
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2374 2668 4793 844
Size: 1530 294 2125 3489
Fragments arranged by size:
3489 2125 1530 294
BsrBI CCG'CTC
Cuts at: 1394 1823 5325 1394
Size: 429 3502 3507
Fragments arranged by size:
3507 3502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2177 4309 5933 6107 65
Size: 1183 929 2132 1624 174 1396
Fragments arranged by size:
2132 1624 1396 1183 929 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6082 6269 1700
Size: 110 4272 187 2869
Fragments arranged by size:
4272 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3690 96
Size: 3594 3844
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 5498 6510 5498
Size: 1012 6426
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3042 4499 794
Size: 2248 1457 3733
Fragments arranged by size:
3733 2248 1457
BstBI TT'CG_AA
Cuts at: 2007 6242 2007
Size: 4235 3203
BstEII G'GTnAC_C
Cuts at: 1184 4237 1184
Size: 3053 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 2466 2840 2466
Size: 374 7064
BstYI r'GATC_y
Cuts at: 1972 3874 4416 4768 5135 5520 5537 6989 7075
Size: 1902 542 352 367 385 17 1452 86
Cuts at: 7075 7087 7433 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1902 1452 542 385 367 352 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2175 2175
Size: 7438
Bsu36I CC'TnA_GG
Cuts at: 4530 4530
Size: 7438
BtgI C'CryG_G
Cuts at: 513 1065 2022 2151 2397 2895 3510 513
Size: 552 957 129 246 498 615 4441
Fragments arranged by size:
4441 957 615 552 498 246 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3457 487
Size: 683 56 2231 4468
Fragments arranged by size:
4468 2231 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 3666 4954 5748 5768 3409
Size: 257 1288 794 20 5079
Fragments arranged by size:
5079 1288 794 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4088 4123 4448 4483 529
Size: 35 3524 35 325 35 3484
Fragments arranged by size:
3524 3484 325 35 35 35
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2922 2958 3105
Size: 54 1425 50 275 18 1092 36 147
Cuts at: 3105 3507 3663 4885 5774 7403 8
Size: 402 156 1222 889 1629 43
Fragments arranged by size:
1629 1425 1222 1092 889 402 275 156
147 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3314 5368 1353
Size: 714 1247 2054 3423
Fragments arranged by size:
3423 2054 1247 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3824 6025 6399 6545 387
Size: 1064 138 2235 2201 374 146 1280
Fragments arranged by size:
2235 2201 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2730 2952 3023 3228 3464 3501 3598
Size: 393 1098 222 71 205 236 37 97
Cuts at: 3598 4472 5501 6098 6885 7153 1239
Size: 874 1029 597 787 268 1524
Fragments arranged by size:
1524 1098 1029 874 787 597 393 268
236 222 205 97 71 37
EcoNI CCTnn'n_nnAGG
Cuts at: 3541 3818 3541
Size: 277 7161
EcoO109I rG'GnC_Cy
Cuts at: 2629 2644 3088 3497 2629
Size: 15 444 409 6570
Fragments arranged by size:
6570 444 409 15
EcoRI G'AATT_C
Cuts at: 3147 4270 4747 5125 3147
Size: 1123 477 378 5460
Fragments arranged by size:
5460 1123 477 378
EcoRV GAT'ATC
Cuts at: 4048 4048
Size: 7438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3028 4066 4702 3028
Size: 1038 636 5764
Fragments arranged by size:
5764 1038 636
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 4436 6585 1678
Size: 2758 2149 2531
Fragments arranged by size:
2758 2531 2149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2566 2598 4659 4691 6079
Size: 32 135 32 994 32 2061 32 1388
Cuts at: 6079 6111 6153 6185 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2061 1388 994 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2638 4782 5028 669
Size: 988 281 700 2144 246 3079
Fragments arranged by size:
3079 2144 988 700 281 246
HindIII A'AGCT_T
Cuts at: 748 4566 748
Size: 3818 3620
HpaI GTT'AAC
Cuts at: 2638 5028 2638
Size: 2390 5048
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 2784 7314 2784
Size: 4530 2908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2447 6552 6736 1305
Size: 1142 4105 184 2007
Fragments arranged by size:
4105 2007 1142 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 3107 3509 10
Size: 54 1425 343 1128 147 402 3939
Fragments arranged by size:
3939 1425 1128 402 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 3269 3434 3659 5396 5755 5914
Size: 648 1162 941 165 225 1737 359 159
Cuts at: 5914 518
Size: 2042
Fragments arranged by size:
2042 1737 1162 941 648 359 225 165
159
MspA1I CmG'CkG
Cuts at: 1606 2529 3379 4168 4288 4854 5531 6679 6924
Size: 923 850 789 120 566 677 1148 245
Cuts at: 6924 1606
Size: 2120
Fragments arranged by size:
2120 1148 923 850 789 677 566 245
120
NcoI C'CATG_G
Cuts at: 513 1065 2022 2397 3510 513
Size: 552 957 375 1113 4441
Fragments arranged by size:
4441 1113 957 552 375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 2827 3073 3713 4796 6341 1132
Size: 97 1598 246 640 1083 1545 2229
Fragments arranged by size:
2229 1598 1545 1083 640 246 97
PasI CC'CwG_GG
Cuts at: 1348 3190 4079 1348
Size: 1842 889 4707
Fragments arranged by size:
4707 1842 889
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2403 3190 3422 1108
Size: 1295 787 232 5124
Fragments arranged by size:
5124 1295 787 232
PfoI T'CCnGG_A
Cuts at: 2122 2122
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PmlI CAC'GTG
Cuts at: 2956 2956
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 3088 2644
Size: 444 6994
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PstI C_TGCA'G
Cuts at: 830 4524 4790 830
Size: 3694 266 3478
Fragments arranged by size:
3694 3478 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2529 4168 4288 2529
Size: 1639 120 5679
Fragments arranged by size:
5679 1639 120
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SanDI GG'GwC_CC
Cuts at: 2644 2644
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 3157 5686 7398 3157
Size: 2529 1712 3197
Fragments arranged by size:
3197 2529 1712
SfcI C'TryA_G
Cuts at: 826 1045 2143 3857 4520 4786 5921 6602 6793
Size: 219 1098 1714 663 266 1135 681 191
Cuts at: 6793 826
Size: 1471
Fragments arranged by size:
1714 1471 1135 1098 681 663 266 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 3314 4604 4765 1872
Size: 1442 1290 161 4545
Fragments arranged by size:
4545 1442 1290 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 5542 6443 6705 820
Size: 197 926 1070 2529 901 262 1553
Fragments arranged by size:
2529 1553 1070 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4735 5229 493
Size: 4242 494 2702
Fragments arranged by size:
4242 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 2827 4796 1132
Size: 1695 1969 3774
Fragments arranged by size:
3774 1969 1695
SspI AAT'ATT
Cuts at: 5 52 2482 5362 5
Size: 47 2430 2880 2081
Fragments arranged by size:
2880 2430 2081 47
StuI AGG'CCT
Cuts at: 1517 2234 3813 4428 1517
Size: 717 1579 615 4527
Fragments arranged by size:
4527 1579 717 615
StyI C'CwwG_G
Cuts at: 513 1065 2022 2397 3510 3542 3808 4877 513
Size: 552 957 375 1113 32 266 1069 3074
Fragments arranged by size:
3074 1113 1069 957 552 375 266 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 3514 4259 4637 5465 5482 5635
Size: 1255 1737 197 745 378 828 17 153
Cuts at: 5635 5820 6265 325
Size: 185 445 1498
Fragments arranged by size:
1737 1498 1255 828 745 445 378 197
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3155 3690 4212
Size: 274 80 33 51 1443 1178 535 522
Cuts at: 4212 5684 7396 96
Size: 1472 1712 138
Fragments arranged by size:
1712 1472 1443 1178 535 522 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2703 2735 4037 4069 4349 4381 4438 4470 7398
Size: 32 1302 32 280 32 57 32 2928
Cuts at: 7398 7430 2703
Size: 32 2711
Fragments arranged by size:
2928 2711 1302 280 57 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4616 1316
Size: 3300 4138
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 3115 2677
Size: 438 7000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 3312 4602 4763 1870
Size: 1442 1290 161 4545
Fragments arranged by size:
4545 1442 1290 161
XmnI GAAnn'nnTTC
Cuts at: 4629 4685 5567 4629
Size: 56 882 6500
Fragments arranged by size:
6500 882 56
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BsiWI DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI