Restriction map for
pFN21ASDA0025
(Circular) (Six-base) MAPSORT of: pFN21ASDA0025.seq Check: 8345 from: 1 to: 5902
pFN21ASDA0025 5902 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2659 2659
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 3245 1937
Size: 217 1091 4594
Fragments arranged by size:
4594 1091 217
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2510 3075 3965 5349 5617 1632
Size: 846 32 565 890 1384 268 1917
Fragments arranged by size:
1917 1384 890 846 565 268 32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2568 1184
Size: 1384 4518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2411 2443 2411
Size: 32 5870
AlwNI CAG_nnn'CTG
Cuts at: 2588 3645 5217 2588
Size: 1057 1572 3273
Fragments arranged by size:
3273 1572 1057
ApaLI G'TGCA_C
Cuts at: 2622 3959 5115 2622
Size: 1337 1156 3409
Fragments arranged by size:
3409 1337 1156
ApoI r'AATT_y
Cuts at: 1493 2749 3211 3437 3589 5698 1493
Size: 1256 462 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 462 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2159 3227 1870
Size: 73 99 117 1068 4545
Fragments arranged by size:
4545 1068 117 99 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 2072 3221 1391
Size: 658 23 1149 4072
Fragments arranged by size:
4072 1149 658 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 3133 928
Size: 971 1164 70 3697
Fragments arranged by size:
3697 1164 971 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2379 2870 3794 5004 2379
Size: 491 924 1210 3277
Fragments arranged by size:
3277 1210 924 491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 2471 4513
Size: 107 122 71 631 605 652 147 2042
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
2042 1525 652 631 605 147 122 107
71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2626 3963 5119 1790
Size: 836 1337 1156 2573
Fragments arranged by size:
2573 1337 1156 836
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2137 2640 2757 3060 4562 1239
Size: 898 503 117 303 1502 2579
Fragments arranged by size:
2579 1502 898 503 303 117
Bpu10I CC'TnA_GC
Cuts at: 2144 2882 2144
Size: 738 5164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2368 2434 2703 4027 4892 5190 2117
Size: 251 66 269 1324 865 298 2829
Fragments arranged by size:
2829 1324 865 298 269 251 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 2647 4565 882
Size: 617 528 620 1918 2219
Fragments arranged by size:
2219 1918 620 617 528
BsaAI yAC'GTr
Cuts at: 493 1204 3199 3693 493
Size: 711 1995 494 2702
Fragments arranged by size:
2702 1995 711 494
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2501 4335 4733 5007 5154 5565 2501
Size: 1834 398 274 147 411 2838
Fragments arranged by size:
2838 1834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2858 2888 1178
Size: 30 165 30 1455 30 4192
Fragments arranged by size:
4192 1455 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2706 2866 2982 1186
Size: 1168 352 160 116 4106
Fragments arranged by size:
4106 1168 352 160 116
BseYI C'CCAG_C
Cuts at: 2466 3181 5105 5601 2466
Size: 715 1924 496 2767
Fragments arranged by size:
2767 1924 715 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2631 1163
Size: 573 895 4434
Fragments arranged by size:
4434 895 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2110 3352 4113 5141 1540
Size: 275 186 109 1242 761 1028 2301
Fragments arranged by size:
2301 1242 1028 761 275 186 109
BsiHKAI G_wGCw'C
Cuts at: 2626 3128 3963 4048 5119 2626
Size: 502 835 85 1071 3409
Fragments arranged by size:
3409 1071 835 502 85
BsmI GAATG_Cn'
Cuts at: 2474 2713 2767 3413 3506 2474
Size: 239 54 646 93 4870
Fragments arranged by size:
4870 646 239 93 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2072 2626 3128 3221 3963 4048
Size: 399 259 23 554 502 93 742 85
Cuts at: 4048 5119 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 554 502 399 259 93
85 23
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2659 2934 3257 844
Size: 1815 275 323 3489
Fragments arranged by size:
3489 1815 323 275
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 4397 4571 65
Size: 1183 1039 2110 174 1396
Fragments arranged by size:
2110 1396 1183 1039 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2962 4546 4733 1700
Size: 110 1152 1584 187 2869
Fragments arranged by size:
2869 1584 1152 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 2588 794
Size: 1472 322 4108
Fragments arranged by size:
4108 1472 322
BstBI TT'CG_AA
Cuts at: 2007 4706 2007
Size: 2699 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897
Size: 1260 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2373 2373
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2022 2110 513
Size: 552 957 88 4305
Fragments arranged by size:
4305 957 552 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3172 487
Size: 683 56 1946 3217
Fragments arranged by size:
3217 1946 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 3418 4212 4232 2586
Size: 832 794 20 4256
Fragments arranged by size:
4256 832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 2621 2771 2621
Size: 150 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867
Size: 54 1425 50 275 18 1519 889 1629
Cuts at: 5867 8
Size: 43
Fragments arranged by size:
1629 1519 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2282 3168 3832 1353
Size: 707 222 886 664 3423
Fragments arranged by size:
3423 886 707 664 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 2478 2510 2640 2757 3060 3075
Size: 393 505 341 32 130 117 303 15
Cuts at: 3075 3965 4562 5349 5617 1239
Size: 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 505 393 341 303
268 130 117 32 15
EcoO109I rG'GnC_Cy
Cuts at: 2489 3013 3176 2489
Size: 524 163 5215
Fragments arranged by size:
5215 524 163
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2083 5049 1678
Size: 405 2966 2531
Fragments arranged by size:
2966 2531 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373
Size: 32 135 32 2971 32 42 32 2626
Fragments arranged by size:
2971 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 2796 5016 5200 1305
Size: 888 603 2220 184 2007
Fragments arranged by size:
2220 2007 888 603 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 3860 4219 4378 518
Size: 648 1734 960 359 159 2042
Fragments arranged by size:
2042 1734 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2684 3318 3995 5143 5388 1606
Size: 506 572 634 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 634 572 506 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4393
Fragments arranged by size:
4393 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2264 2572 3260 4805 1132
Size: 97 1035 308 688 1545 2229
Fragments arranged by size:
2229 1545 1035 688 308 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2485 2984 1108
Size: 1377 499 4026
Fragments arranged by size:
4026 1377 499
PfoI T'CCnGG_A
Cuts at: 2734 2734
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 3013 2489
Size: 524 5378
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2684 2684
Size: 5902
SacII CC_GC'GG
Cuts at: 2113 2113
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5902
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 2564 4150 5862 2564
Size: 1586 1712 2604
Fragments arranged by size:
2604 1712 1586
SfcI C'TryA_G
Cuts at: 826 1045 3250 4385 5066 5257 826
Size: 219 2205 1135 681 191 1471
Fragments arranged by size:
2205 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2383 2449 2718 4006 4907
Size: 197 926 189 251 66 269 1288 901
Cuts at: 4907 5169 820
Size: 262 1553
Fragments arranged by size:
1553 1288 926 901 269 262 251 197
189 66
SnaBI TAC'GTA
Cuts at: 493 3199 3693 493
Size: 2706 494 2702
Fragments arranged by size:
2706 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2576 3826 5
Size: 47 2524 1250 2081
Fragments arranged by size:
2524 2081 1250 47
StuI AGG'CCT
Cuts at: 1517 2455 2484 1517
Size: 938 29 4935
Fragments arranged by size:
4935 938 29
StyI C'CwwG_G
Cuts at: 513 1065 2022 2319 2479 3341 513
Size: 552 957 297 160 862 3074
Fragments arranged by size:
3074 957 862 552 297 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 2508 2928 2971 3929 3946 4099
Size: 1255 471 457 420 43 958 17 153
Cuts at: 4099 4284 4729 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 958 471 457 445 420 185
153 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3088 4148
Size: 274 80 33 51 1443 585 526 1060
Cuts at: 4148 5860 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1060 585 526 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2412 4031 2412
Size: 1619 4283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI