Restriction map for
pFN21ASDA0020
(Circular) (Six-base) MAPSORT of: pFN21ASDA0020.seq Check: 4060 from: 1 to: 6253
pFN21ASDA0020 6253 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4063 278
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2670 3596 1937
Size: 733 926 4594
Fragments arranged by size:
4594 926 733
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 3392 4316 5700 5968 1632
Size: 1664 96 924 1384 268 1917
Fragments arranged by size:
1917 1664 1384 924 268 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 5152 6129 1203
Size: 3949 977 1327
Fragments arranged by size:
3949 1327 977
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3255 3287 3359 3391 3255
Size: 32 72 32 6117
Fragments arranged by size:
6117 72 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3996 5568 3996
Size: 1572 4681
ApaLI G'TGCA_C
Cuts at: 4310 5466 4310
Size: 1156 5097
ApoI r'AATT_y
Cuts at: 1493 2264 3522 3562 3788 3940 6049 1493
Size: 771 1258 40 226 152 2109 1697
Fragments arranged by size:
2109 1697 1258 771 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 3578 1870
Size: 73 1635 4545
Fragments arranged by size:
4545 1635 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3015 3048 3015
Size: 33 6220
BamHI G'GATC_C
Cuts at: 3583 3950 3583
Size: 367 5886
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2693 3398 3574 5029 618
Size: 325 217 627 906 705 176 1455 1842
Fragments arranged by size:
1842 1455 906 705 627 325 217 176
BanII G_rGCy'C
Cuts at: 1391 2427 3048 3572 1391
Size: 1036 621 524 4072
Fragments arranged by size:
4072 1036 621 524
BbeI G_GCGC'C
Cuts at: 2697 2697
Size: 6253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2771 928
Size: 971 872 4410
Fragments arranged by size:
4410 971 872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4444 4478 1903
Size: 34 2507 34 3678
Fragments arranged by size:
3678 2507 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2702 3197 4864
Size: 107 122 71 631 605 1030 495 1667
Cuts at: 4864 136
Size: 1525
Fragments arranged by size:
1667 1525 1030 631 605 495 122 107
71
BglII A'GATC_T
Cuts at: 6248 6248
Size: 6253
BlpI GC'TnA_GC
Cuts at: 3670 3670
Size: 6253
Bme1580I G_kGCm'C
Cuts at: 1790 4314 5470 1790
Size: 2524 1156 2573
Fragments arranged by size:
2573 2524 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3984 4942 442
Size: 416 53 83 399 24 2567 958 1753
Fragments arranged by size:
2567 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3048 3180 3407 4913 1239
Size: 1809 132 227 1506 2579
Fragments arranged by size:
2579 1809 1506 227 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4378 5243 5541 4378
Size: 865 298 5090
Fragments arranged by size:
5090 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4916 882
Size: 617 3417 2219
Fragments arranged by size:
3417 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2326 3550 4044 493
Size: 711 1122 1224 494 2702
Fragments arranged by size:
2702 1224 1122 711 494
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 4060 4442 5077
Size: 53 83 186 936 1161 1366 382 635
Cuts at: 5077 275
Size: 1451
Fragments arranged by size:
1451 1366 1161 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4686 5084 5358 5505 5916 4686
Size: 398 274 147 411 5023
Fragments arranged by size:
5023 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3030 3060 3310 3340 1178
Size: 30 165 30 1627 30 250 30 4091
Fragments arranged by size:
4091 1627 250 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3410 1186
Size: 2224 4029
BseYI C'CCAG_C
Cuts at: 5456 5952 5456
Size: 496 5757
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2940 3447 1163
Size: 573 1204 507 3969
Fragments arranged by size:
3969 1204 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3048 3545 4314 4399 5470 2427
Size: 621 497 769 85 1071 3210
Fragments arranged by size:
3210 1071 769 621 497 85
BsmI GAATG_Cn'
Cuts at: 3187 3764 3857 3187
Size: 577 93 5583
Fragments arranged by size:
5583 577 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3156 3156
Size: 6253
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 3048 3545 3572 4314 4399 5470
Size: 399 637 621 497 27 742 85 1071
Cuts at: 5470 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 637 621 497 399 85
27
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 4142 5872 4142
Size: 1730 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3090 3608 844
Size: 2246 518 3489
Fragments arranged by size:
3489 2246 518
BsrBI CCG'CTC
Cuts at: 1394 1823 4140 1394
Size: 429 2317 3507
Fragments arranged by size:
3507 2317 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2885 3243 4748 4922 65
Size: 1183 1637 358 1505 174 1396
Fragments arranged by size:
1637 1505 1396 1183 358 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4897 5084 1700
Size: 110 3087 187 2869
Fragments arranged by size:
3087 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 2142 4313 5325 2142
Size: 2171 1012 3070
Fragments arranged by size:
3070 2171 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6253
BstBI TT'CG_AA
Cuts at: 2007 5057 2007
Size: 3050 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6253
BstYI r'GATC_y
Cuts at: 1972 2950 3149 3583 3950 4335 4352 5804 5890
Size: 978 199 434 367 385 17 1452 86
Cuts at: 5890 5902 6248 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 978 434 385 367 346 199
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3172 487
Size: 683 56 1946 3568
Fragments arranged by size:
3568 1946 683 56
BtsI GCAGTG_nn'
Cuts at: 2710 3769 4563 4583 2710
Size: 1059 794 20 4380
Fragments arranged by size:
4380 1059 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 2317 3557 2317
Size: 1240 5013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5260 809
Size: 507 3944 1802
Fragments arranged by size:
3944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 2791 3700
Size: 54 1425 50 275 18 873 88 909
Cuts at: 3700 4589 6218 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 909 889 873 275 88 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 4183 1353
Size: 1062 1768 3423
Fragments arranged by size:
3423 1768 1062
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2425 3046 2425
Size: 621 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3048 3180 3296 3392 3407 4316 4913
Size: 393 1416 132 116 96 15 909 597
Cuts at: 4913 5700 5968 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1416 909 787 597 393 268 132
116 96 15
EcoO109I rG'GnC_Cy
Cuts at: 2923 2923
Size: 6253
EcoRI G'AATT_C
Cuts at: 3562 3940 3562
Size: 378 5875
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 2697 5400 1678
Size: 1019 2703 2531
Fragments arranged by size:
2703 2531 1019
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2527 2559 3030 3062 4894
Size: 32 135 32 955 32 471 32 1832
Cuts at: 4894 4926 4968 5000 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1832 955 471 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3597 3843 669
Size: 988 281 1659 246 3079
Fragments arranged by size:
3079 1659 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2846 3472 748
Size: 2098 626 3529
Fragments arranged by size:
3529 2098 626
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KasI G'GCGC_C
Cuts at: 2693 2693
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5367 5551 1305
Size: 4062 184 2007
Fragments arranged by size:
4062 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 2793 10
Size: 54 1425 343 873 88 3470
Fragments arranged by size:
3470 1425 873 343 88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 4211 4570 4729 518
Size: 648 2053 992 359 159 2042
Fragments arranged by size:
2053 2042 992 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3669 4346 5494 5739 1606
Size: 2063 677 1148 245 2120
Fragments arranged by size:
2120 2063 1148 677 245
NarI GG'CG_CC
Cuts at: 2694 2694
Size: 6253
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5701
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2348 2413 3356 3611 5156 1132
Size: 97 1119 65 943 255 1545 2229
Fragments arranged by size:
2229 1545 1119 943 255 97 65
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6253
PciI A'CATG_T
Cuts at: 5152 5152
Size: 6253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3103 1108
Size: 1995 4258
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PmlI CAC'GTG
Cuts at: 2326 2326
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2923 2923
Size: 6253
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PstI C_TGCA'G
Cuts at: 830 3262 3605 830
Size: 2432 343 3478
Fragments arranged by size:
3478 2432 343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
SacI G_AGCT'C
Cuts at: 2427 3048 2427
Size: 621 5632
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SapI GCTCTTCn'nnn_
Cuts at: 2415 2415
Size: 6253
SbfI CC_TGCA'GG
Cuts at: 3262 3605 3262
Size: 343 5910
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 3258 3601 4736 5417 5608 826
Size: 219 2213 343 1135 681 191 1471
Fragments arranged by size:
2213 1471 1135 681 343 219 191
SfoI GGC'GCC
Cuts at: 2695 2695
Size: 6253
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4357 5258 5520 820
Size: 197 926 2414 901 262 1553
Fragments arranged by size:
2414 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3550 4044 493
Size: 3057 494 2702
Fragments arranged by size:
3057 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 2348 3611 1132
Size: 1216 1263 3774
Fragments arranged by size:
3774 1263 1216
SspI AAT'ATT
Cuts at: 5 52 2243 2254 4177 5
Size: 47 2191 11 1923 2081
Fragments arranged by size:
2191 2081 1923 47 11
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6253
StyI C'CwwG_G
Cuts at: 513 1065 2733 3692 513
Size: 552 1668 959 3074
Fragments arranged by size:
3074 1668 959 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4280 4297 4450 4635 5080 325
Size: 1255 2700 17 153 185 445 1498
Fragments arranged by size:
2700 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 4499 6211
Size: 274 80 33 51 1443 954 1568 1712
Cuts at: 6211 96
Size: 138
Fragments arranged by size:
1712 1568 1443 954 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6213 6245 6213
Size: 32 6221
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6253
XbaI T'CTAG_A
Cuts at: 3589 3589
Size: 6253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3193 3193
Size: 6253
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 4382 4382
Size: 6253
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4061 276
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI