Restriction map for
pFN21ASDA0007
(Circular) (Six-base) MAPSORT of: pFN21ASDA0007.seq Check: 8853 from: 1 to: 6760
pFN21ASDA0007 6760 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4570 278
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 4103 1937
Size: 685 1481 4594
Fragments arranged by size:
4594 1481 685
Acc65I G'GTAC_C
Cuts at: 2201 3655 4081 2201
Size: 1454 426 4880
Fragments arranged by size:
4880 1454 426
AclI AA'CG_TT
Cuts at: 1563 4887 5260 1563
Size: 3324 373 3063
Fragments arranged by size:
3324 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3240 3855 3894 4823 6207 6475 1632
Size: 1608 615 39 929 1384 268 1917
Fragments arranged by size:
1917 1608 1384 929 615 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6563
AflIII A'CryG_T
Cuts at: 1203 5659 6636 1203
Size: 4456 977 1327
Fragments arranged by size:
4456 1327 977
AgeI A'CCGG_T
Cuts at: 2751 5591 2751
Size: 2840 3920
AhdI GACnn_n'nnGTC
Cuts at: 3055 5489 3055
Size: 2434 4326
AleI CACnn'nnGTG
Cuts at: 1166 2196 1166
Size: 1030 5730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6760
AlwNI CAG_nnn'CTG
Cuts at: 2282 2439 4503 6075 2282
Size: 157 2064 1572 2967
Fragments arranged by size:
2967 2064 1572 157
ApaI G_GGCC'C
Cuts at: 3169 3169
Size: 6760
ApaLI G'TGCA_C
Cuts at: 4817 5973 4817
Size: 1156 5604
ApoI r'AATT_y
Cuts at: 1493 3214 4069 4295 4447 6556 1493
Size: 1721 855 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 855 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2381 5314 160
Size: 872 1349 2933 1606
Fragments arranged by size:
2933 1606 1349 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
AvaI C'yCGr_G
Cuts at: 1870 1943 4085 1870
Size: 73 2142 4545
Fragments arranged by size:
4545 2142 73
BamHI G'GATC_C
Cuts at: 4090 4457 4090
Size: 367 6393
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2224 3235 3655 4081
Size: 325 217 627 414 23 1011 420 426
Cuts at: 4081 5536 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1011 627 426 420 414 325
217 23
BanII G_rGCy'C
Cuts at: 1391 3169 4079 1391
Size: 1778 910 4072
Fragments arranged by size:
4072 1778 910
BbeI G_GCGC'C
Cuts at: 2228 2228
Size: 6760
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3393 3503 3779 3822 928
Size: 971 1494 110 276 43 3866
Fragments arranged by size:
3866 1494 971 276 110 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4951 4985 1903
Size: 34 3014 34 3678
Fragments arranged by size:
3678 3014 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4652 5862 4652
Size: 1210 5550
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6760
BfrBI ATG'CAT
Cuts at: 3626 3626
Size: 6760
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2233 5371 136
Size: 107 122 71 631 605 561 3138 1525
Fragments arranged by size:
3138 1525 631 605 561 122 107 71
BglII A'GATC_T
Cuts at: 6755 6755
Size: 6760
BlpI GC'TnA_GC
Cuts at: 4177 4177
Size: 6760
Bme1580I G_kGCm'C
Cuts at: 1790 3169 4821 5977 1790
Size: 1379 1652 1156 2573
Fragments arranged by size:
2573 1652 1379 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6760
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4491 5449 442
Size: 416 53 83 399 24 3074 958 1753
Fragments arranged by size:
3074 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2757 3103 3377 5420 1239
Size: 1518 346 274 2043 2579
Fragments arranged by size:
2579 2043 1518 346 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4885 5750 6048 4885
Size: 865 298 5597
Fragments arranged by size:
5597 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2603 5423 882
Size: 617 1104 2820 2219
Fragments arranged by size:
2820 2219 1104 617
BsaAI yAC'GTr
Cuts at: 493 1204 2179 4057 4551 493
Size: 711 975 1878 494 2702
Fragments arranged by size:
2702 1878 975 711 494
BsaBI GATnn'nnATC
Cuts at: 6754 6754
Size: 6760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 4567 4949 5584
Size: 53 83 186 936 692 2342 382 635
Cuts at: 5584 275
Size: 1451
Fragments arranged by size:
2342 1451 936 692 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2751 5193 5591 5865 6012 6423 2751
Size: 2442 398 274 147 411 3088
Fragments arranged by size:
3088 2442 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6535
Fragments arranged by size:
6535 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3267 3854 3911 3914 1186
Size: 2081 587 57 3 4032
Fragments arranged by size:
4032 2081 587 57 3
BseYI C'CCAG_C
Cuts at: 2066 3162 3660 3664 5963 6459 2066
Size: 1096 498 4 2299 496 2367
Fragments arranged by size:
2367 2299 1096 498 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2191 2820 2950 1163
Size: 573 414 41 629 130 4973
Fragments arranged by size:
4973 629 573 414 130 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4210 4971 5999 1540
Size: 275 186 2209 761 1028 2301
Fragments arranged by size:
2301 2209 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4821 4906 5977 4821
Size: 85 1071 5604
Fragments arranged by size:
5604 1071 85
BsmI GAATG_Cn'
Cuts at: 4271 4364 4271
Size: 93 6667
BsmBI CGTCTCn'nnnn_
Cuts at: 2141 2141
Size: 6760
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3169 4079 4821 4906 5977 1391
Size: 399 1379 910 742 85 1071 2174
Fragments arranged by size:
2174 1379 1071 910 742 399 85
BspEI T'CCGG_A
Cuts at: 6423 6423
Size: 6760
BspHI T'CATG_A
Cuts at: 4649 6379 4649
Size: 1730 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2213 2761 4115 844
Size: 1369 548 1354 3489
Fragments arranged by size:
3489 1369 1354 548
BsrBI CCG'CTC
Cuts at: 1394 1823 3275 4647 1394
Size: 429 1452 1372 3507
Fragments arranged by size:
3507 1452 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3360 5255 5429 65
Size: 1183 2112 1895 174 1396
Fragments arranged by size:
2112 1895 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 2751 5404 5591 1700
Size: 110 350 591 2653 187 2869
Fragments arranged by size:
2869 2653 591 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6760
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6760
BssSI C'ACGA_G
Cuts at: 4820 5832 4820
Size: 1012 5748
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6760
BstBI TT'CG_AA
Cuts at: 2007 5564 2007
Size: 3557 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6760
BstYI r'GATC_y
Cuts at: 1972 4027 4090 4457 4842 4859 6311 6397 6409
Size: 2055 63 367 385 17 1452 86 12
Cuts at: 6409 6755 1972
Size: 346 1977
Fragments arranged by size:
2055 1977 1452 385 367 346 86 63
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5251
Fragments arranged by size:
5251 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6021
Fragments arranged by size:
6021 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2808 4276 5070 5090 2536
Size: 272 1468 794 20 4206
Fragments arranged by size:
4206 1468 794 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6725
DraI TTT'AAA
Cuts at: 3114 4064 3114
Size: 950 5810
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 5767 809
Size: 507 1089 3362 1802
Fragments arranged by size:
3362 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 4207 5096
Size: 54 1425 50 275 18 321 2056 889
Cuts at: 5096 6725 8
Size: 1629 43
Fragments arranged by size:
2056 1629 1425 889 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4207 1537
Size: 275 2395 4090
Fragments arranged by size:
4090 2395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2862 3819 3831 3927 4690 1353
Size: 1509 957 12 96 763 3423
Fragments arranged by size:
3423 1509 957 763 96 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5347 5721 5867 387
Size: 1064 138 3758 374 146 1280
Fragments arranged by size:
3758 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2757 3103 3240 3377 3855 3894 4823
Size: 393 1125 346 137 137 478 39 929
Cuts at: 4823 5420 6207 6475 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1125 929 787 597 478 393 346
268 137 137 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2991 2991
Size: 6760
EcoO109I rG'GnC_Cy
Cuts at: 2071 3166 2071
Size: 1095 5665
EcoRI G'AATT_C
Cuts at: 3214 4069 4447 3214
Size: 855 378 5527
Fragments arranged by size:
5527 855 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2930 2930
Size: 6760
FspI TGC'GCA
Cuts at: 5266 5266
Size: 6760
HaeII r_GCGC'y
Cuts at: 1678 2228 5907 1678
Size: 550 3679 2531
Fragments arranged by size:
3679 2531 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 3235 3267 5401
Size: 32 135 32 1469 32 162 32 2134
Cuts at: 5401 5433 5475 5507 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2134 1469 162 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 3006 3615 4104 4350 669
Size: 988 281 472 596 609 489 246 3079
Fragments arranged by size:
3079 988 609 596 489 472 281 246
HindIII A'AGCT_T
Cuts at: 748 3734 748
Size: 2986 3774
HpaI GTT'AAC
Cuts at: 3615 4350 3615
Size: 735 6025
KasI G'GCGC_C
Cuts at: 2224 2224
Size: 6760
KpnI G_GTAC'C
Cuts at: 2205 3659 4085 2205
Size: 1454 426 4880
Fragments arranged by size:
4880 1454 426
MfeI C'AATT_G
Cuts at: 4359 4359
Size: 6760
MluI A'CGCG_T
Cuts at: 6636 6636
Size: 6760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5874 6058 1305
Size: 4569 184 2007
Fragments arranged by size:
4569 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4938
Fragments arranged by size:
4938 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 3726 4718 5077 5236 518
Size: 648 1030 1530 992 359 159 2042
Fragments arranged by size:
2042 1530 1030 992 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2579 3664 4176 4853 6001 6246 1606
Size: 973 1085 512 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 973 677 512 245
NarI GG'CG_CC
Cuts at: 2225 2225
Size: 6760
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5251
Fragments arranged by size:
5251 957 552
NdeI CA'TA_TG
Cuts at: 387 3132 387
Size: 2745 4015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6760
NotI GC'GGCC_GC
Cuts at: 4207 4207
Size: 6760
NsiI A_TGCA'T
Cuts at: 3628 3628
Size: 6760
NspI r_CATG'y
Cuts at: 1132 1229 2496 2645 4118 5663 1132
Size: 97 1267 149 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 1267 149 97
PasI CC'CwG_GG
Cuts at: 1348 3424 1348
Size: 2076 4684
PciI A'CATG_T
Cuts at: 5659 5659
Size: 6760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3685 1108
Size: 2577 4183
PmeI GTTT'AAAC
Cuts at: 4064 4064
Size: 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 6342 6374 4829
Size: 32 1481 32 5215
Fragments arranged by size:
5215 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 6760
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6760
PsiI TTA'TAA
Cuts at: 3491 4330 3491
Size: 839 5921
PspOMI G'GGCC_C
Cuts at: 3165 3165
Size: 6760
PstI C_TGCA'G
Cuts at: 830 2241 4112 830
Size: 1411 1871 3478
Fragments arranged by size:
3478 1871 1411
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6760
PvuII CAG'CTG
Cuts at: 2579 3664 2579
Size: 1085 5675
SalI G'TCGA_C
Cuts at: 1936 4102 1936
Size: 2166 4594
SanDI GG'GwC_CC
Cuts at: 2071 2071
Size: 6760
SapI GCTCTTCn'nnn_
Cuts at: 2862 2862
Size: 6760
SbfI CC_TGCA'GG
Cuts at: 4112 4112
Size: 6760
ScaI AGT'ACT
Cuts at: 3465 5008 6720 3465
Size: 1543 1712 3505
Fragments arranged by size:
3505 1712 1543
SexAI A'CCwGG_T
Cuts at: 3651 3651
Size: 6760
SfcI C'TryA_G
Cuts at: 826 1045 2237 2283 2623 4108 5243 5924 6115
Size: 219 1192 46 340 1485 1135 681 191
Cuts at: 6115 826
Size: 1471
Fragments arranged by size:
1485 1471 1192 1135 681 340 219 191
46
SfoI GGC'GCC
Cuts at: 2226 2226
Size: 6760
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6760
SmaI CCC'GGG
Cuts at: 1872 4087 1872
Size: 2215 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4864 5765 6027 820
Size: 197 926 2921 901 262 1553
Fragments arranged by size:
2921 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4057 4551 493
Size: 3564 494 2702
Fragments arranged by size:
3564 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6760
SphI G_CATG'C
Cuts at: 1132 4118 1132
Size: 2986 3774
SspI AAT'ATT
Cuts at: 5 52 4684 5
Size: 47 4632 2081
Fragments arranged by size:
4632 2081 47
StuI AGG'CCT
Cuts at: 1517 2102 1517
Size: 585 6175
StyI C'CwwG_G
Cuts at: 513 1065 2022 2244 4199 513
Size: 552 957 222 1955 3074
Fragments arranged by size:
3074 1955 957 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4787 4804 4957 5142 5587 325
Size: 1255 3207 17 153 185 445 1498
Fragments arranged by size:
3207 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2743 3463 5006
Size: 274 80 33 51 1443 766 720 1543
Cuts at: 5006 6718 96
Size: 1712 138
Fragments arranged by size:
1712 1543 1443 766 720 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6720 6752 6720
Size: 32 6728
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6760
XbaI T'CTAG_A
Cuts at: 4096 4096
Size: 6760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2705
Size: 6760
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6760
XmaI C'CCGG_G
Cuts at: 1870 4085 1870
Size: 2215 4545
XmnI GAAnn'nnTTC
Cuts at: 4889 4889
Size: 6760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4568 276
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI