Restriction map for
pFN21AF0386
(Circular) (Six-base) MAPSORT of: pFN21AF0386.seq Check: 6408 from: 1 to: 6727
pFN21AF0386 6727 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2251 2974 2251
Size: 723 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4537 278
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 4070 1937
Size: 864 1269 4594
Fragments arranged by size:
4594 1269 864
Acc65I G'GTAC_C
Cuts at: 4048 4048
Size: 6727
AclI AA'CG_TT
Cuts at: 1563 4854 5227 1563
Size: 3291 373 3063
Fragments arranged by size:
3291 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 3148 3290 3428 4790 6174 6442 1632
Size: 533 983 142 138 1362 1384 268 1917
Fragments arranged by size:
1917 1384 1362 983 533 268 142 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6530
AflIII A'CryG_T
Cuts at: 1203 5626 6603 1203
Size: 4423 977 1327
Fragments arranged by size:
4423 1327 977
AgeI A'CCGG_T
Cuts at: 5558 5558
Size: 6727
AhdI GACnn_n'nnGTC
Cuts at: 5456 5456
Size: 6727
AleI CACnn'nnGTG
Cuts at: 1166 3197 1166
Size: 2031 4696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6727
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 6695
AlwNI CAG_nnn'CTG
Cuts at: 2059 2494 2638 2650 3259 4470 6042 2059
Size: 435 144 12 609 1211 1572 2744
Fragments arranged by size:
2744 1572 1211 609 435 144 12
ApaI G_GGCC'C
Cuts at: 3994 3994
Size: 6727
ApaLI G'TGCA_C
Cuts at: 3640 3917 4784 5940 3640
Size: 277 867 1156 4427
Fragments arranged by size:
4427 1156 867 277
ApoI r'AATT_y
Cuts at: 1493 2819 4036 4262 4414 6523 1493
Size: 1326 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5281 160
Size: 872 4249 1606
Fragments arranged by size:
4249 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 4052 1870
Size: 73 214 1895 4545
Fragments arranged by size:
4545 1895 214 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3491 3524 3491
Size: 33 6694
BamHI G'GATC_C
Cuts at: 4057 4424 4057
Size: 367 6360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3463 3487 4048 5503 618
Size: 325 217 627 1676 24 561 1455 1842
Fragments arranged by size:
1842 1676 1455 627 561 325 217 24
BanII G_rGCy'C
Cuts at: 1391 2833 3137 3224 3297 3831 3994 4046 1391
Size: 1442 304 87 73 534 163 52 4072
Fragments arranged by size:
4072 1442 534 304 163 87 73 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3339 3416 3553 928
Size: 971 1440 77 137 4102
Fragments arranged by size:
4102 1440 971 137 77
BbvCI CC'TCA_GC
Cuts at: 3243 3243
Size: 6727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 4918 4952 1903
Size: 34 887 34 2060 34 3678
Fragments arranged by size:
3678 2060 887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4619 5829 4619
Size: 1210 5517
BclI T'GATC_A
Cuts at: 1548 3309 1548
Size: 1761 4966
BfrBI ATG'CAT
Cuts at: 2662 2662
Size: 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3228 3539 5338
Size: 107 122 71 631 605 1556 311 1799
Cuts at: 5338 136
Size: 1525
Fragments arranged by size:
1799 1556 1525 631 605 311 122 107
71
BglII A'GATC_T
Cuts at: 6722 6722
Size: 6727
BlpI GC'TnA_GC
Cuts at: 2358 4144 2358
Size: 1786 4941
Bme1580I G_kGCm'C
Cuts at: 1790 2245 3468 3644 3840 3921 3994 4788 5944
Size: 455 1223 176 196 81 73 794 1156
Cuts at: 5944 1790
Size: 2573
Fragments arranged by size:
2573 1223 1156 794 455 196 176 81
73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6727
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2849 3087 3131
Size: 416 53 83 399 24 1432 238 44
Cuts at: 3131 3626 3681 4458 5416 442
Size: 495 55 777 958 1753
Fragments arranged by size:
1753 1432 958 777 495 416 399 238
83 55 53 44 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2399 2691 3217 3458 3680 3845 3893
Size: 1001 159 292 526 241 222 165 48
Cuts at: 3893 3919 5387 1239
Size: 26 1468 2579
Fragments arranged by size:
2579 1468 1001 526 292 241 222 165
159 48 26
Bpu10I CC'TnA_GC
Cuts at: 2901 3243 2901
Size: 342 6385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 3431 3680 4852 5717 6015 2211
Size: 1220 249 1172 865 298 2923
Fragments arranged by size:
2923 1220 1172 865 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 5390 882
Size: 617 519 3372 2219
Fragments arranged by size:
3372 2219 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 4518 493
Size: 711 3314 2702
Fragments arranged by size:
3314 2702 711
BsaBI GATnn'nnATC
Cuts at: 6721 6721
Size: 6727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3894 4534 4916 5551
Size: 53 83 186 936 2361 640 382 635
Cuts at: 5551 275
Size: 1451
Fragments arranged by size:
2361 1451 936 640 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5160 5558 5832 5979 6390 5160
Size: 398 274 147 411 5497
Fragments arranged by size:
5497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2919 2949 3239 3269 1178
Size: 30 165 30 1516 30 290 30 4636
Fragments arranged by size:
4636 1516 290 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3260 1186
Size: 2074 4653
BseYI C'CCAG_C
Cuts at: 2244 2580 2643 2697 2754 3018 3044 3107 3206
Size: 336 63 54 57 264 26 63 99
Cuts at: 3206 3223 5930 6426 2244
Size: 17 2707 496 2545
Fragments arranged by size:
2707 2545 496 336 264 99 63 63
57 54 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 2864 2879 2891 3008 1163
Size: 573 681 447 15 12 117 4882
Fragments arranged by size:
4882 681 573 447 117 15 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4177 4938 5966 1540
Size: 275 186 2176 761 1028 2301
Fragments arranged by size:
2301 2176 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2833 3644 3696 3831 3921 4788 4873 5944
Size: 791 811 52 135 90 867 85 1071
Cuts at: 5944 2042
Size: 2825
Fragments arranged by size:
2825 1071 867 811 791 135 90 85
52
BsmI GAATG_Cn'
Cuts at: 4238 4331 4238
Size: 93 6634
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2245 2833 3137 3224 3297 3468
Size: 399 252 203 588 304 87 73 171
Cuts at: 3468 3644 3696 3831 3840 3921 3994 4046 4788
Size: 176 52 135 9 81 73 52 742
Cuts at: 4788 4873 5944 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 304 252 203
176 171 135 87 85 81 73 73
52 52 9
BspEI T'CCGG_A
Cuts at: 6390 6390
Size: 6727
BspHI T'CATG_A
Cuts at: 3216 4616 6346 3216
Size: 1400 1730 3597
Fragments arranged by size:
3597 1730 1400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2251 2974 4082 844
Size: 1407 723 1108 3489
Fragments arranged by size:
3489 1407 1108 723
BsrBI CCG'CTC
Cuts at: 1394 1823 3523 3552 3673 4614 1394
Size: 429 1700 29 121 941 3507
Fragments arranged by size:
3507 1700 941 429 121 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 5222 5396 65
Size: 1183 3974 174 1396
Fragments arranged by size:
3974 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3876 5371 5558 1700
Size: 110 2066 1495 187 2869
Fragments arranged by size:
2869 2066 1495 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6727
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6727
BssSI C'ACGA_G
Cuts at: 2177 4787 5799 2177
Size: 2610 1012 3105
Fragments arranged by size:
3105 2610 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2572 2659 3004 794
Size: 1778 87 345 4517
Fragments arranged by size:
4517 1778 345 87
BstBI TT'CG_AA
Cuts at: 5531 5531
Size: 6727
BstEII G'GTnAC_C
Cuts at: 1184 3967 1184
Size: 2783 3944
BstXI CCAn_nnnn'nTGG
Cuts at: 3730 3730
Size: 6727
BstYI r'GATC_y
Cuts at: 1972 4057 4424 4809 4826 6278 6364 6376 6722
Size: 2085 367 385 17 1452 86 12 346
Cuts at: 6722 1972
Size: 1977
Fragments arranged by size:
2085 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2802 2802
Size: 6727
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5236
Fragments arranged by size:
5236 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5988
Fragments arranged by size:
5988 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 3887 4243 5037 5057 3751
Size: 136 356 794 20 5421
Fragments arranged by size:
5421 794 356 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6692
DraI TTT'AAA
Cuts at: 3189 4031 3189
Size: 842 5885
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2998 5734 809
Size: 507 1682 2736 1802
Fragments arranged by size:
2736 1802 1682 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 3037 3540
Size: 54 1425 50 275 18 351 856 503
Cuts at: 3540 3708 4174 5063 6692 8
Size: 168 466 889 1629 43
Fragments arranged by size:
1629 1425 889 856 503 466 351 275
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4174 1537
Size: 275 2362 4090
Fragments arranged by size:
4090 2362 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3356 4657 1353
Size: 2003 1301 3423
Fragments arranged by size:
3423 2003 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2050 2613 5314 5688 5834 387
Size: 1064 138 461 563 2701 374 146 1280
Fragments arranged by size:
2701 1280 1064 563 461 374 146 138
EcoICRI GAG'CTC
Cuts at: 2831 3829 2831
Size: 998 5729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2240 2399 2691 3148 3217 3290
Size: 393 533 75 159 292 457 69 73
Cuts at: 3290 3428 3458 3680 3845 3893 3919 4790 5387
Size: 138 30 222 165 48 26 871 597
Cuts at: 5387 6174 6442 1239
Size: 787 268 1524
Fragments arranged by size:
1524 871 787 597 533 457 393 292
268 222 165 159 138 75 73 69
48 30 26
EcoO109I rG'GnC_Cy
Cuts at: 3346 3802 3346
Size: 456 6271
EcoRI G'AATT_C
Cuts at: 4036 4414 4036
Size: 378 6349
FspI TGC'GCA
Cuts at: 5233 5233
Size: 6727
HaeII r_GCGC'y
Cuts at: 1678 2270 2321 5874 1678
Size: 592 51 3553 2531
Fragments arranged by size:
3553 2531 592 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2905 2919 2937 2951 2995
Size: 32 135 32 1333 14 18 14 44
Cuts at: 2995 3027 3947 3979 5368 5400 5442 5474 1373
Size: 32 920 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1389 1333 920 135 44 42 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4071 4317 669
Size: 988 281 2133 246 3079
Fragments arranged by size:
3079 2133 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2229 748
Size: 1481 5246
HpaI GTT'AAC
Cuts at: 4317 4317
Size: 6727
KpnI G_GTAC'C
Cuts at: 4052 4052
Size: 6727
MfeI C'AATT_G
Cuts at: 4326 4326
Size: 6727
MluI A'CGCG_T
Cuts at: 6603 6603
Size: 6727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2545 3087 5841 6025 1305
Size: 1240 542 2754 184 2007
Fragments arranged by size:
2754 2007 1240 542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 3542 3710 10
Size: 54 1425 343 351 1359 168 3027
Fragments arranged by size:
3027 1425 1359 351 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3197 3461 4685 5044 5203 518
Size: 648 2031 264 1224 359 159 2042
Fragments arranged by size:
2042 2031 1224 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2494 2512 2650 3227 3277 3842 3865
Size: 642 246 18 138 577 50 565 23
Cuts at: 3865 3924 4143 4820 5968 6213 1606
Size: 59 219 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 577 565 246 245
219 138 59 50 23 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5236
Fragments arranged by size:
5236 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6727
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6727
NotI GC'GGCC_GC
Cuts at: 4174 4174
Size: 6727
NsiI A_TGCA'T
Cuts at: 2664 2664
Size: 6727
NspI r_CATG'y
Cuts at: 1132 1229 4085 5630 1132
Size: 97 2856 1545 2229
Fragments arranged by size:
2856 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6727
PciI A'CATG_T
Cuts at: 5626 5626
Size: 6727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 2761 2917 1108
Size: 1155 498 156 4918
Fragments arranged by size:
4918 1155 498 156
PfoI T'CCnGG_A
Cuts at: 2923 3525 3951 2923
Size: 602 426 5699
Fragments arranged by size:
5699 602 426
PmeI GTTT'AAAC
Cuts at: 4031 4031
Size: 6727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 4796 4828 6309 6341 3194
Size: 32 1570 32 1481 32 3580
Fragments arranged by size:
3580 1570 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3346 3802 3346
Size: 456 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6727
PsiI TTA'TAA
Cuts at: 4297 4297
Size: 6727
PspOMI G'GGCC_C
Cuts at: 3990 3990
Size: 6727
PstI C_TGCA'G
Cuts at: 830 2253 2274 2499 2517 2655 3758 3948 4079
Size: 1423 21 225 18 138 1103 190 131
Cuts at: 4079 830
Size: 3478
Fragments arranged by size:
3478 1423 1103 225 190 138 131 21
18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6727
PvuII CAG'CTG
Cuts at: 2248 2494 2512 2650 3227 3277 2248
Size: 246 18 138 577 50 5698
Fragments arranged by size:
5698 577 246 138 50 18
SacI G_AGCT'C
Cuts at: 2833 3831 2833
Size: 998 5729
SalI G'TCGA_C
Cuts at: 1936 4069 1936
Size: 2133 4594
SapI GCTCTTCn'nnn_
Cuts at: 3356 3356
Size: 6727
SbfI CC_TGCA'GG
Cuts at: 4079 4079
Size: 6727
ScaI AGT'ACT
Cuts at: 4975 6687 4975
Size: 1712 5015
SfcI C'TryA_G
Cuts at: 826 1045 2249 2270 2321 2495 2513 2651 3754
Size: 219 1204 21 51 174 18 138 1103
Cuts at: 3754 3944 4075 5210 5891 6082 826
Size: 190 131 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 1103 681 219 191 190
174 138 131 51 21 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6727
SmaI CCC'GGG
Cuts at: 1872 2159 4054 1872
Size: 287 1895 4545
Fragments arranged by size:
4545 1895 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 3446 3659 4831 5732 5994
Size: 197 926 283 1220 213 1172 901 262
Cuts at: 5994 820
Size: 1553
Fragments arranged by size:
1553 1220 1172 926 901 283 262 213
197
SnaBI TAC'GTA
Cuts at: 493 4518 493
Size: 4025 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6727
SphI G_CATG'C
Cuts at: 1132 4085 1132
Size: 2953 3774
SspI AAT'ATT
Cuts at: 5 52 4651 5
Size: 47 4599 2081
Fragments arranged by size:
4599 2081 47
StuI AGG'CCT
Cuts at: 1517 4010 1517
Size: 2493 4234
StyI C'CwwG_G
Cuts at: 513 1065 2004 4166 513
Size: 552 939 2162 3074
Fragments arranged by size:
3074 2162 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3634 3955 4754 4771 4924 5109
Size: 1255 462 1592 321 799 17 153 185
Cuts at: 5109 5554 325
Size: 445 1498
Fragments arranged by size:
1592 1498 1255 799 462 445 321 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2890 3285 4973
Size: 274 80 33 51 1443 913 395 1688
Cuts at: 4973 6685 96
Size: 1712 138
Fragments arranged by size:
1712 1688 1443 913 395 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2096 2112 2128 3574 3606 6687 6719 2080
Size: 16 16 16 1446 32 3081 32 2088
Fragments arranged by size:
3081 2088 1446 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6727
XbaI T'CTAG_A
Cuts at: 4063 4063
Size: 6727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 2122
Size: 6727
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6727
XmaI C'CCGG_G
Cuts at: 1870 2157 4052 1870
Size: 287 1895 4545
Fragments arranged by size:
4545 1895 287
XmnI GAAnn'nnTTC
Cuts at: 4856 4856
Size: 6727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4535 276
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BplI BsiWI BsmBI Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI