Restriction map for
pFN21AF0293
(Circular) (Six-base) MAPSORT of: pFN21AF0293.seq Check: 7699 from: 1 to: 7759
pFN21AF0293 7759 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 3999 4373 4374 2300
Size: 1699 374 1 5685
Fragments arranged by size:
5685 1699 374 1
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3576 5569 278
Size: 53 83 186 936 2040 1993 2468
Fragments arranged by size:
2468 2040 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 5102 1937
Size: 748 2417 4594
Fragments arranged by size:
4594 2417 748
Acc65I G'GTAC_C
Cuts at: 2199 5080 2199
Size: 2881 4878
AclI AA'CG_TT
Cuts at: 1563 5886 6259 1563
Size: 4323 373 3063
Fragments arranged by size:
4323 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2779 2823 3308 4407 5822 7206 7474 1632
Size: 1147 44 485 1099 1415 1384 268 1917
Fragments arranged by size:
1917 1415 1384 1147 1099 485 268 44
AfeI AGC'GCT
Cuts at: 4021 4021
Size: 7759
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7562
AflIII A'CryG_T
Cuts at: 1203 6658 7635 1203
Size: 5455 977 1327
Fragments arranged by size:
5455 1327 977
AgeI A'CCGG_T
Cuts at: 2277 6590 2277
Size: 4313 3446
AhdI GACnn_n'nnGTC
Cuts at: 4522 6488 4522
Size: 1966 5793
AleI CACnn'nnGTG
Cuts at: 1166 2432 2592 3362 3707 1166
Size: 1266 160 770 345 5218
Fragments arranged by size:
5218 1266 770 345 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4396 1184
Size: 3212 4547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 7727
AlwNI CAG_nnn'CTG
Cuts at: 2167 3091 3463 5502 7074 2167
Size: 924 372 2039 1572 2852
Fragments arranged by size:
2852 2039 1572 924 372
ApaI G_GGCC'C
Cuts at: 4396 4468 4513 4669 4396
Size: 72 45 156 7486
Fragments arranged by size:
7486 156 72 45
ApaLI G'TGCA_C
Cuts at: 2726 5816 6972 2726
Size: 3090 1156 3513
Fragments arranged by size:
3513 3090 1156
ApoI r'AATT_y
Cuts at: 1493 5068 5294 5446 7555 1493
Size: 3575 226 152 2109 1697
Fragments arranged by size:
3575 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6313 160
Size: 872 5281 1606
Fragments arranged by size:
5281 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7759
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 2958 3001 3057 3135 4512 5084
Size: 73 628 387 43 56 78 1377 572
Cuts at: 5084 1870
Size: 4545
Fragments arranged by size:
4545 1377 628 572 387 78 73 56
43
BamHI G'GATC_C
Cuts at: 3145 5089 5456 3145
Size: 1944 367 5448
Fragments arranged by size:
5448 1944 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2199 3431 4172 4968 5080
Size: 325 217 627 412 1232 741 796 112
Cuts at: 5080 6535 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1232 796 741 627 412 325
217 112
BanII G_rGCy'C
Cuts at: 1391 2217 3792 4001 4126 4255 4396 4468 4513
Size: 826 1575 209 125 129 141 72 45
Cuts at: 4513 4669 4989 5010 5078 1391
Size: 156 320 21 68 4072
Fragments arranged by size:
4072 1575 826 320 209 156 141 129
125 72 68 45 21
BbeI G_GCGC'C
Cuts at: 4972 4972
Size: 7759
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2333 928
Size: 971 434 6354
Fragments arranged by size:
6354 971 434
BbvCI CC'TCA_GC
Cuts at: 2391 3042 4202 2391
Size: 651 1160 5948
Fragments arranged by size:
5948 1160 651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4460 4494 5950 5984 1903
Size: 34 2523 34 1456 34 3678
Fragments arranged by size:
3678 2523 1456 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5651 6861 5651
Size: 1210 6549
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7759
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2490 2918
Size: 107 122 71 631 605 592 226 428
Cuts at: 2918 3688 4451 4463 6370 136
Size: 770 763 12 1907 1525
Fragments arranged by size:
1907 1525 770 763 631 605 592 428
226 122 107 71 12
BglII A'GATC_T
Cuts at: 7754 7754
Size: 7759
BlpI GC'TnA_GC
Cuts at: 2940 4263 4566 4760 5176 2940
Size: 1323 303 194 416 5523
Fragments arranged by size:
5523 1323 416 303 194
Bme1580I G_kGCm'C
Cuts at: 1790 2730 4175 4396 4468 4513 4669 4740 5820
Size: 940 1445 221 72 45 156 71 1080
Cuts at: 5820 6976 1790
Size: 1156 2573
Fragments arranged by size:
2573 1445 1156 1080 940 221 156 72
71 45
BmgBI CAC'GTC
Cuts at: 1318 2413 4054 1318
Size: 1095 1641 5023
Fragments arranged by size:
5023 1641 1095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4498 5490 6448
Size: 416 53 83 399 24 3081 992 958
Cuts at: 6448 442
Size: 1753
Fragments arranged by size:
3081 1753 992 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7759
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 3635 4145 2729
Size: 906 510 6343
Fragments arranged by size:
6343 906 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 2464 2582 3908 4049 4130 4940 6419
Size: 1220 5 118 1326 141 81 810 1479
Cuts at: 6419 1239
Size: 2579
Fragments arranged by size:
2579 1479 1326 1220 810 141 118 81
5
Bpu10I CC'TnA_GC
Cuts at: 2096 2391 3042 3950 4202 2096
Size: 295 651 908 252 5653
Fragments arranged by size:
5653 908 651 295 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2564 4763 5884 6749 7047 2405
Size: 159 2199 1121 865 298 3117
Fragments arranged by size:
3117 2199 1121 865 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4773 6422 882
Size: 617 3274 1649 2219
Fragments arranged by size:
3274 2219 1649 617
BsaAI yAC'GTr
Cuts at: 493 1204 2590 3579 5550 493
Size: 711 1386 989 1971 2702
Fragments arranged by size:
2702 1971 1386 989 711
BsaBI GATnn'nnATC
Cuts at: 7753 7753
Size: 7759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3573 4969 5566 5948
Size: 53 83 186 936 2040 1396 597 382
Cuts at: 5948 6583 275
Size: 635 1451
Fragments arranged by size:
2040 1451 1396 936 635 597 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2277 3549 4233 4656 6192 6590 6864 7011 7422
Size: 1272 684 423 1536 398 274 147 411
Cuts at: 7422 2277
Size: 2614
Fragments arranged by size:
2614 1536 1272 684 423 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3764 3794 1178
Size: 30 165 30 2361 30 5143
Fragments arranged by size:
5143 2361 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 2930 4791 1186
Size: 1705 39 1861 4154
Fragments arranged by size:
4154 1861 1705 39
BseYI C'CCAG_C
Cuts at: 3833 3994 5051 6962 7458 3833
Size: 161 1057 1911 496 4134
Fragments arranged by size:
4134 1911 1057 496 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 4781 4817 1163
Size: 573 909 2136 36 4105
Fragments arranged by size:
4105 2136 909 573 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2535 4847 5209 5970 6998 1540
Size: 275 186 534 2312 362 761 1028 2301
Fragments arranged by size:
2312 2301 1028 761 534 362 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2730 2973 4614 5820 5905 6976 2217
Size: 513 243 1641 1206 85 1071 3000
Fragments arranged by size:
3000 1641 1206 1071 513 243 85
BsmI GAATG_Cn'
Cuts at: 5270 5363 5270
Size: 93 7666
BsmBI CGTCTCn'nnnn_
Cuts at: 2864 4936 2864
Size: 2072 5687
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2730 2973 3792 4001 4126 4175
Size: 399 427 513 243 819 209 125 49
Cuts at: 4175 4255 4396 4468 4513 4614 4669 4740 4989
Size: 80 141 72 45 101 55 71 249
Cuts at: 4989 5010 5078 5820 5905 6976 1391
Size: 21 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 819 742 513 427 399 249
243 209 141 125 101 85 80 72
71 68 55 49 45 21
BspEI T'CCGG_A
Cuts at: 3549 4233 4656 7422 3549
Size: 684 423 2766 3886
Fragments arranged by size:
3886 2766 684 423
BspHI T'CATG_A
Cuts at: 2079 5648 7378 2079
Size: 3569 1730 2460
Fragments arranged by size:
3569 2460 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2300 3999 4373 4374 5114 844
Size: 1253 203 1699 374 1 740 3489
Fragments arranged by size:
3489 1699 1253 740 374 203 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 2976 3379 4086 4839 5646 1394
Size: 429 401 752 403 707 753 807 3507
Fragments arranged by size:
3507 807 753 752 707 429 403 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 2458 6254 6428 65
Size: 1183 1026 184 3796 174 1396
Fragments arranged by size:
3796 1396 1183 1026 184 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2277 4710 6403 6590 1700
Size: 110 467 2433 1693 187 2869
Fragments arranged by size:
2869 2433 1693 467 187 110
BsrGI T'GTAC_A
Cuts at: 96 3645 96
Size: 3549 4210
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7759
BssSI C'ACGA_G
Cuts at: 2966 4820 5819 6831 2966
Size: 1854 999 1012 3894
Fragments arranged by size:
3894 1854 1012 999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2350 2659 4042 794
Size: 1556 309 1383 4511
Fragments arranged by size:
4511 1556 1383 309
BstBI TT'CG_AA
Cuts at: 6563 6563
Size: 7759
BstEII G'GTnAC_C
Cuts at: 1184 3709 1184
Size: 2525 5234
BstXI CCAn_nnnn'nTGG
Cuts at: 3364 3364
Size: 7759
BstYI r'GATC_y
Cuts at: 1972 3145 3367 4653 5089 5456 5841 5858 7310
Size: 1173 222 1286 436 367 385 17 1452
Cuts at: 7310 7396 7408 7754 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1286 1173 436 385 367 346
222 86 17 12
BstZ17I GTA'TAC
Cuts at: 2686 2686
Size: 7759
Bsu36I CC'TnA_GG
Cuts at: 2184 2184
Size: 7759
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 3388 3528 3585 3753 4458
Size: 552 939 63 1321 140 57 168 705
Cuts at: 4458 4804 4980 513
Size: 346 176 3292
Fragments arranged by size:
3292 1321 939 705 552 346 176 168
140 63 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 2344 2807 4940 487
Size: 683 56 950 168 463 2133 3306
Fragments arranged by size:
3306 2133 950 683 463 168 56
BtsI GCAGTG_nn'
Cuts at: 2641 2772 2913 3033 3084 4894 5275 6069 6089
Size: 131 141 120 51 1810 381 794 20
Cuts at: 6089 2641
Size: 4311
Fragments arranged by size:
4311 1810 794 381 141 131 120 51
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7724
DraI TTT'AAA
Cuts at: 5063 5063
Size: 7759
DraIII CAC_nnn'GTG
Cuts at: 4246 4246
Size: 7759
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6766 809
Size: 507 5450 1802
Fragments arranged by size:
5450 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2225 2247 2910
Size: 54 1425 50 275 18 395 22 663
Cuts at: 2910 2949 3399 3538 3680 4063 4325 4452 4807
Size: 39 450 139 142 383 262 127 355
Cuts at: 4807 4844 4887 5206 6095 7724 8
Size: 37 43 319 889 1629 43
Fragments arranged by size:
1629 1425 889 663 450 395 383 355
319 275 262 142 139 127 54 50
43 43 39 37 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 4844 5206 1537
Size: 275 3032 362 4090
Fragments arranged by size:
4090 3032 362 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 3297 3626 3746 4382 5689 1353
Size: 1446 498 329 120 636 1307 3423
Fragments arranged by size:
3423 1446 1307 636 498 329 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6346 6720 6866 387
Size: 1064 138 4757 374 146 1280
Fragments arranged by size:
4757 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 7759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 2464 2582 2779 2823 3308 3908
Size: 393 827 5 118 197 44 485 600
Cuts at: 3908 4049 4130 4407 4940 5822 6419 7206 7474
Size: 141 81 277 533 882 597 787 268
Cuts at: 7474 1239
Size: 1524
Fragments arranged by size:
1524 882 827 787 600 597 533 485
393 277 268 197 141 118 81 44
5
EcoNI CCTnn'n_nnAGG
Cuts at: 3290 3623 4180 3290
Size: 333 557 6869
Fragments arranged by size:
6869 557 333
EcoO109I rG'GnC_Cy
Cuts at: 2021 2527 2575 2899 2962 4464 4465 4666 2021
Size: 506 48 324 63 1502 1 201 5114
Fragments arranged by size:
5114 1502 506 324 201 63 48 1
EcoRI G'AATT_C
Cuts at: 5068 5446 5068
Size: 378 7381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3808 3808
Size: 7759
FspI TGC'GCA
Cuts at: 6265 6265
Size: 7759
HaeII r_GCGC'y
Cuts at: 1678 3721 4023 4192 4972 6906 1678
Size: 2043 302 169 780 1934 2531
Fragments arranged by size:
2531 2043 1934 780 302 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2529 2561 2798 2830 3933
Size: 32 135 32 957 32 237 32 1103
Cuts at: 3933 3965 4508 4540 6400 6432 6474 6506 1373
Size: 32 543 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1860 1103 957 543 237 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3217 5103 5349 669
Size: 988 281 1279 1886 246 3079
Fragments arranged by size:
3079 1886 1279 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7759
HpaI GTT'AAC
Cuts at: 5349 5349
Size: 7759
KasI G'GCGC_C
Cuts at: 4968 4968
Size: 7759
KpnI G_GTAC'C
Cuts at: 2203 5084 2203
Size: 2881 4878
MfeI C'AATT_G
Cuts at: 2621 5358 2621
Size: 2737 5022
MluI A'CGCG_T
Cuts at: 7635 7635
Size: 7759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4026 4959 6873 7057 1305
Size: 2721 933 1914 184 2007
Fragments arranged by size:
2721 2007 1914 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 4065 10
Size: 54 1425 343 417 1816 3704
Fragments arranged by size:
3704 1816 1425 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2432 2592 2723 3179 3362 3707
Size: 648 1072 194 160 131 456 183 345
Cuts at: 3707 4142 4220 4979 5717 6076 6235 518
Size: 435 78 759 738 359 159 2042
Fragments arranged by size:
2042 1072 759 738 648 456 435 359
345 194 183 160 159 131 78
MspA1I CmG'CkG
Cuts at: 1606 2650 3041 3088 3804 4273 4330 4402 4773
Size: 1044 391 47 716 469 57 72 371
Cuts at: 4773 4786 4806 5019 5175 5852 7000 7245 1606
Size: 13 20 213 156 677 1148 245 2120
Fragments arranged by size:
2120 1148 1044 716 677 469 391 371
245 213 156 72 57 47 20 13
NarI GG'CG_CC
Cuts at: 4969 4969
Size: 7759
NcoI C'CATG_G
Cuts at: 513 1065 2004 2067 3528 3753 4458 513
Size: 552 939 63 1461 225 705 3814
Fragments arranged by size:
3814 1461 939 705 552 225 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7759
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7759
NotI GC'GGCC_GC
Cuts at: 5206 5206
Size: 7759
NspI r_CATG'y
Cuts at: 1132 1229 4432 5117 6662 1132
Size: 97 3203 685 1545 2229
Fragments arranged by size:
3203 2229 1545 685 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7759
PciI A'CATG_T
Cuts at: 6658 6658
Size: 7759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3994 4789 1108
Size: 2886 795 4078
Fragments arranged by size:
4078 2886 795
PfoI T'CCnGG_A
Cuts at: 3624 4026 4356 3624
Size: 402 330 7027
Fragments arranged by size:
7027 402 330
PmeI GTTT'AAAC
Cuts at: 5063 5063
Size: 7759
PmlI CAC'GTG
Cuts at: 2590 2590
Size: 7759
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5828 5860 7341 7373 3764
Size: 32 2032 32 1481 32 4150
Fragments arranged by size:
4150 2032 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2575 2962 2527
Size: 48 387 7324
Fragments arranged by size:
7324 387 48
PshAI GACnn'nnGTC
Cuts at: 1613 4100 1613
Size: 2487 5272
PsiI TTA'TAA
Cuts at: 5329 5329
Size: 7759
PspOMI G'GGCC_C
Cuts at: 4392 4464 4509 4665 4392
Size: 72 45 156 7486
Fragments arranged by size:
7486 156 72 45
PstI C_TGCA'G
Cuts at: 830 2094 2892 3040 3264 3422 3459 4047 4272
Size: 1264 798 148 224 158 37 588 225
Cuts at: 4272 4350 4385 5111 830
Size: 78 35 726 3478
Fragments arranged by size:
3478 1264 798 726 588 225 224 158
148 78 37 35
PvuI CG_AT'CG
Cuts at: 2001 2535 2001
Size: 534 7225
PvuII CAG'CTG
Cuts at: 2650 3041 4402 4786 2650
Size: 391 1361 384 5623
Fragments arranged by size:
5623 1361 391 384
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 7759
SacII CC_GC'GG
Cuts at: 4807 4807
Size: 7759
SalI G'TCGA_C
Cuts at: 1936 5101 1936
Size: 3165 4594
SanDI GG'GwC_CC
Cuts at: 2527 2527
Size: 7759
SbfI CC_TGCA'GG
Cuts at: 5111 5111
Size: 7759
ScaI AGT'ACT
Cuts at: 6007 7719 6007
Size: 1712 6047
SexAI A'CCwGG_T
Cuts at: 3864 3864
Size: 7759
SfcI C'TryA_G
Cuts at: 826 1045 2090 2888 3036 3260 3418 3455 4043
Size: 219 1045 798 148 224 158 37 588
Cuts at: 4043 4268 4346 4381 5107 6242 6923 7114 826
Size: 225 78 35 726 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 798 726 681 588 225
224 219 191 158 148 78 37 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 4451 4451
Size: 7759
SfoI GGC'GCC
Cuts at: 4970 4970
Size: 7759
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7759
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7759
SmaI CCC'GGG
Cuts at: 1872 2960 3059 3137 5086 1872
Size: 1088 99 78 1949 4545
Fragments arranged by size:
4545 1949 1088 99 78
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 2579 4742 5863 6764 7026
Size: 197 926 477 159 2163 1121 901 262
Cuts at: 7026 820
Size: 1553
Fragments arranged by size:
2163 1553 1121 926 901 477 262 197
159
SnaBI TAC'GTA
Cuts at: 493 5550 493
Size: 5057 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7759
SphI G_CATG'C
Cuts at: 1132 5117 1132
Size: 3985 3774
SspI AAT'ATT
Cuts at: 5 52 5683 5
Size: 47 5631 2081
Fragments arranged by size:
5631 2081 47
StuI AGG'CCT
Cuts at: 1517 2444 3416 1517
Size: 927 972 5860
Fragments arranged by size:
5860 972 927
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2736 3072 3528 3753 3783
Size: 552 939 63 669 336 456 225 30
Cuts at: 3783 4176 4446 4458 5198 513
Size: 393 270 12 740 3074
Fragments arranged by size:
3074 939 740 669 552 456 393 336
270 225 63 30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 2786 3091 3459 5786 5803 5956
Size: 1255 1002 204 305 368 2327 17 153
Cuts at: 5956 6141 6586 325
Size: 185 445 1498
Fragments arranged by size:
2327 1498 1255 1002 445 368 305 204
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 2496 3645
Size: 274 80 33 51 1443 378 141 1149
Cuts at: 3645 6005 7717 96
Size: 2360 1712 138
Fragments arranged by size:
2360 1712 1443 1149 378 274 141 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3106 3138 4525 4557 7719 7751 3106
Size: 32 1387 32 3162 32 3114
Fragments arranged by size:
3162 3114 1387 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3170 1316
Size: 1854 5905
XbaI T'CTAG_A
Cuts at: 5095 5095
Size: 7759
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2845 3367 3760 3991 2434
Size: 411 522 393 231 6202
Fragments arranged by size:
6202 522 411 393 231
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7759
XmaI C'CCGG_G
Cuts at: 1870 2958 3057 3135 5084 1870
Size: 1088 99 78 1949 4545
Fragments arranged by size:
4545 1949 1088 99 78
XmnI GAAnn'nnTTC
Cuts at: 4643 5888 4643
Size: 1245 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3574 5567 276
Size: 53 83 186 936 2040 1993 2468
Fragments arranged by size:
2468 2040 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SapI SrfI SwaI