Restriction map for
pFN21AF0229
(Circular) (Six-base) MAPSORT of: pFN21AF0229.seq Check: 7902 from: 1 to: 6529
pFN21AF0229 6529 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2685 2755 2685
Size: 70 6459
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4339 278
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3669 3872 1937
Size: 1732 203 4594
Fragments arranged by size:
4594 1732 203
Acc65I G'GTAC_C
Cuts at: 3850 3850
Size: 6529
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2953 3191 4592 5976 6244 1632
Size: 1321 238 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1321 268 238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6332
AflIII A'CryG_T
Cuts at: 1203 2717 5428 6405 1203
Size: 1514 2711 977 1327
Fragments arranged by size:
2711 1514 1327 977
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 5258 5258
Size: 6529
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6529
AlwNI CAG_nnn'CTG
Cuts at: 2927 4272 5844 2927
Size: 1345 1572 3612
Fragments arranged by size:
3612 1572 1345
ApaI G_GGCC'C
Cuts at: 2021 2037 2021
Size: 16 6513
ApaLI G'TGCA_C
Cuts at: 4586 5742 4586
Size: 1156 5373
ApoI r'AATT_y
Cuts at: 1493 2317 3008 3141 3446 3541 3838 4064 4216
Size: 824 691 133 305 95 297 226 152
Cuts at: 4216 6325 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 824 691 305 297 226 152
133 95
AscI GG'CGCG_CC
Cuts at: 2115 2115
Size: 6529
AseI AT'TA_AT
Cuts at: 160 1032 5083 160
Size: 872 4051 1606
Fragments arranged by size:
4051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 3854 1870
Size: 73 1911 4545
Fragments arranged by size:
4545 1911 73
BamHI G'GATC_C
Cuts at: 2440 3639 3859 4226 2440
Size: 1199 220 367 4743
Fragments arranged by size:
4743 1199 367 220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2899 3850 5305 618
Size: 325 217 627 302 810 951 1455 1842
Fragments arranged by size:
1842 1455 951 810 627 325 302 217
BanII G_rGCy'C
Cuts at: 1391 2021 2037 2886 3848 1391
Size: 630 16 849 962 4072
Fragments arranged by size:
4072 962 849 630 16
BbeI G_GCGC'C
Cuts at: 2093 2903 2093
Size: 810 5719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 928
Size: 971 459 5099
Fragments arranged by size:
5099 971 459
BbvCI CC'TCA_GC
Cuts at: 2253 3477 2253
Size: 1224 5305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3692 3726 4720 4754 1903
Size: 34 1755 34 994 34 3678
Fragments arranged by size:
3678 1755 994 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 2529 1548
Size: 981 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2102 5140
Size: 107 122 71 631 605 353 77 3038
Cuts at: 5140 136
Size: 1525
Fragments arranged by size:
3038 1525 631 605 353 122 107 77
71
BglII A'GATC_T
Cuts at: 6524 6524
Size: 6529
BlpI GC'TnA_GC
Cuts at: 3946 3946
Size: 6529
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2037 4590 5746 1790
Size: 231 16 2553 1156 2573
Fragments arranged by size:
2573 2553 1156 231 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 2648 2828
Size: 416 53 83 399 24 918 313 180
Cuts at: 2828 4260 5218 442
Size: 1432 958 1753
Fragments arranged by size:
1753 1432 958 918 416 399 313 180
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3435 3435
Size: 6529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3074 3222 5189 1239
Size: 1001 834 148 1967 2579
Fragments arranged by size:
2579 1967 1001 834 148
Bpu10I CC'TnA_GC
Cuts at: 2253 3147 3477 2253
Size: 894 330 5305
Fragments arranged by size:
5305 894 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3482 4654 5519 5817 3482
Size: 1172 865 298 4194
Fragments arranged by size:
4194 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3114 5192 882
Size: 617 1615 2078 2219
Fragments arranged by size:
2219 2078 1615 617
BsaAI yAC'GTr
Cuts at: 493 1204 4320 493
Size: 711 3116 2702
Fragments arranged by size:
3116 2702 711
BsaBI GATnn'nnATC
Cuts at: 2637 6523 2637
Size: 3886 2643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 2900 4336 4718
Size: 53 83 186 936 557 810 1436 382
Cuts at: 4718 5353 275
Size: 635 1451
Fragments arranged by size:
1451 1436 936 810 635 557 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4962 5360 5634 5781 6192 4962
Size: 398 274 147 411 5299
Fragments arranged by size:
5299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 3022 3052 1178
Size: 30 165 30 759 30 830 30 4655
Fragments arranged by size:
4655 830 759 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2389 1186
Size: 1203 5326
BseYI C'CCAG_C
Cuts at: 2166 2224 5732 6228 2166
Size: 58 3508 496 2467
Fragments arranged by size:
3508 2467 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2771 1163
Size: 573 438 597 4921
Fragments arranged by size:
4921 597 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3979 4740 5768 1540
Size: 275 186 1978 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 3720 4590 4675 5746 2571
Size: 1149 870 85 1071 3354
Fragments arranged by size:
3354 1149 1071 870 85
BsmI GAATG_Cn'
Cuts at: 2257 4040 4133 2257
Size: 1783 93 4653
Fragments arranged by size:
4653 1783 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2037 2571 2886 3720 3848 4590
Size: 399 231 16 534 315 834 128 742
Cuts at: 4590 4675 5746 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 834 742 534 399 315 231
128 85 16
BspEI T'CCGG_A
Cuts at: 6192 6192
Size: 6529
BspHI T'CATG_A
Cuts at: 2722 2803 2998 4418 6148 2722
Size: 81 195 1420 1730 3103
Fragments arranged by size:
3103 1730 1420 195 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 2755 3142 3884 844
Size: 1841 70 387 742 3489
Fragments arranged by size:
3489 1841 742 387 70
BsrBI CCG'CTC
Cuts at: 1394 1823 4416 1394
Size: 429 2593 3507
Fragments arranged by size:
3507 2593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3782 5024 5198 65
Size: 1183 2534 1242 174 1396
Fragments arranged by size:
2534 1396 1242 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 3519 5173 5360 1700
Size: 110 282 1427 1654 187 2869
Fragments arranged by size:
2869 1654 1427 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 3271 96
Size: 3175 3354
BssHII G'CGCG_C
Cuts at: 1925 2115 1925
Size: 190 6339
BssSI C'ACGA_G
Cuts at: 4589 5601 4589
Size: 1012 5517
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6529
BstBI TT'CG_AA
Cuts at: 3112 5333 3112
Size: 2221 4308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6529
BstYI r'GATC_y
Cuts at: 1972 2440 3639 3859 4226 4611 4628 6080 6166
Size: 468 1199 220 367 385 17 1452 86
Cuts at: 6166 6178 6524 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1199 468 385 367 346 220
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 2235 3404 513
Size: 552 939 142 89 1169 3638
Fragments arranged by size:
3638 1169 939 552 142 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5790
Fragments arranged by size:
5790 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 4045 4839 4859 2191
Size: 1854 794 20 3861
Fragments arranged by size:
3861 1854 794 20
ClaI AT'CG_AT
Cuts at: 1554 2641 1554
Size: 1087 5442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6494
DraI TTT'AAA
Cuts at: 3231 3833 3231
Size: 602 5927
DraIII CAC_nnn'GTG
Cuts at: 2197 2566 2197
Size: 369 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 809
Size: 507 4220 1802
Fragments arranged by size:
4220 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2149 3098
Size: 54 1425 50 275 18 313 6 949
Cuts at: 3098 3976 4865 6494 8
Size: 878 889 1629 43
Fragments arranged by size:
1629 1425 949 889 878 313 275 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3976 1537
Size: 275 2164 4090
Fragments arranged by size:
4090 2164 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3237 3404 3615 4459 1353
Size: 774 1110 167 211 844 3423
Fragments arranged by size:
3423 1110 844 774 211 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2170 3030 5116 5490 5636 387
Size: 1064 138 581 860 2086 374 146 1280
Fragments arranged by size:
2086 1280 1064 860 581 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2953 3074 3191 3222 4592 5189
Size: 393 608 713 121 117 31 1370 597
Cuts at: 5189 5976 6244 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1370 787 713 608 597 393 268
121 117 31
EcoO109I rG'GnC_Cy
Cuts at: 2017 2018 2033 2155 3473 2017
Size: 1 15 122 1318 5073
Fragments arranged by size:
5073 1318 122 15 1
EcoRI G'AATT_C
Cuts at: 3008 3838 4216 3008
Size: 830 378 5321
Fragments arranged by size:
5321 830 378
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 2093 2903 5676 1678
Size: 415 810 2773 2531
Fragments arranged by size:
2773 2531 810 415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 5170 5202 5244
Size: 32 135 32 590 32 2976 32 42
Cuts at: 5244 5276 1373
Size: 32 2626
Fragments arranged by size:
2976 2626 590 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 2944 3873 4119 669
Size: 988 281 819 187 929 246 3079
Fragments arranged by size:
3079 988 929 819 281 246 187
HindIII A'AGCT_T
Cuts at: 748 3373 748
Size: 2625 3904
HpaI GTT'AAC
Cuts at: 2757 2944 4119 2757
Size: 187 1175 5167
Fragments arranged by size:
5167 1175 187
KasI G'GCGC_C
Cuts at: 2089 2899 2089
Size: 810 5719
KpnI G_GTAC'C
Cuts at: 3854 3854
Size: 6529
MfeI C'AATT_G
Cuts at: 4128 4128
Size: 6529
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2497 3004 5643 5827 1305
Size: 729 463 507 2639 184 2007
Fragments arranged by size:
2639 2007 729 507 463 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3100 10
Size: 54 1425 343 1268 3439
Fragments arranged by size:
3439 1425 1268 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3003 3765 4487 4846 5005 518
Size: 648 1524 313 762 722 359 159 2042
Fragments arranged by size:
2042 1524 762 722 648 359 313 159
MspA1I CmG'CkG
Cuts at: 1606 3945 4622 5770 6015 1606
Size: 2339 677 1148 245 2120
Fragments arranged by size:
2339 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2094 2094
Size: 6529
NarI GG'CG_CC
Cuts at: 2090 2900 2090
Size: 810 5719
NcoI C'CATG_G
Cuts at: 513 1065 2004 3404 513
Size: 552 939 1400 3638
Fragments arranged by size:
3638 1400 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6529
NgoMIV G'CCGG_C
Cuts at: 2092 2092
Size: 6529
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NspI r_CATG'y
Cuts at: 1132 1229 2721 3887 5432 1132
Size: 97 1492 1166 1545 2229
Fragments arranged by size:
2229 1545 1492 1166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6529
PciI A'CATG_T
Cuts at: 2717 5428 2717
Size: 2711 3818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 3589 1108
Size: 1128 1353 4048
Fragments arranged by size:
4048 1353 1128
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 6529
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3473 3473
Size: 6529
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 4099 4099
Size: 6529
PspOMI G'GGCC_C
Cuts at: 2017 2033 2017
Size: 16 6513
PstI C_TGCA'G
Cuts at: 830 3881 830
Size: 3051 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SbfI CC_TGCA'GG
Cuts at: 3881 3881
Size: 6529
ScaI AGT'ACT
Cuts at: 2966 4777 6489 2966
Size: 1811 1712 3006
Fragments arranged by size:
3006 1811 1712
SfcI C'TryA_G
Cuts at: 826 1045 3132 3877 5012 5693 5884 826
Size: 219 2087 745 1135 681 191 1471
Fragments arranged by size:
2087 1471 1135 745 681 219 191
SfoI GGC'GCC
Cuts at: 2091 2901 2091
Size: 810 5719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6529
SmaI CCC'GGG
Cuts at: 1872 3856 1872
Size: 1984 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3461 4633 5534 5796 820
Size: 197 926 1518 1172 901 262 1553
Fragments arranged by size:
1553 1518 1172 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4320 493
Size: 3827 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6529
SphI G_CATG'C
Cuts at: 1132 3887 1132
Size: 2755 3774
SspI AAT'ATT
Cuts at: 5 52 2290 4453 5
Size: 47 2238 2163 2081
Fragments arranged by size:
2238 2163 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 3404 3968 513
Size: 552 939 516 884 564 3074
Fragments arranged by size:
3074 939 884 564 552 516
SwaI ATTT'AAAT
Cuts at: 3231 3231
Size: 6529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4556 4573 4726 4911 5356 325
Size: 1255 2976 17 153 185 445 1498
Fragments arranged by size:
2976 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2964 3271 4775
Size: 274 80 33 51 1443 987 307 1504
Cuts at: 4775 6487 96
Size: 1712 138
Fragments arranged by size:
1712 1504 1443 987 307 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6489 6521 6489
Size: 32 6497
Tth111I GACn'n_nGTC
Cuts at: 1316 3674 1316
Size: 2358 4171
XbaI T'CTAG_A
Cuts at: 3488 3604 3865 3488
Size: 116 261 6152
Fragments arranged by size:
6152 261 116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6529
XmaI C'CCGG_G
Cuts at: 1870 3854 1870
Size: 1984 4545
XmnI GAAnn'nnTTC
Cuts at: 4658 4658
Size: 6529
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4337 276
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI XcmI