Restriction map for
pFN21AF0190
(Circular) (Six-base) MAPSORT of: pFN21AF0190.seq Check: 7452 from: 1 to: 6265
pFN21AF0190 6265 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2660 2660
Size: 6265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2200 3608 1937
Size: 263 1408 4594
Fragments arranged by size:
4594 1408 263
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 2285 2492 3144 3395 4328 5712 5980
Size: 466 187 207 652 251 933 1384 268
Cuts at: 5980 1632
Size: 1917
Fragments arranged by size:
1917 1384 933 652 466 268 251 207
187
AfeI AGC'GCT
Cuts at: 2021 2021
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2299 2610 5164 6141 1203
Size: 1096 311 2554 977 1327
Fragments arranged by size:
2554 1327 1096 977 311
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2278 3171 3418 4008 5580 2278
Size: 893 247 590 1572 2963
Fragments arranged by size:
2963 1572 893 590 247
ApaI G_GGCC'C
Cuts at: 2016 2030 2803 2935 2016
Size: 14 773 132 5346
Fragments arranged by size:
5346 773 132 14
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3290 3574 3800 3952 6061 1493
Size: 1797 284 226 152 2109 1697
Fragments arranged by size:
2109 1797 1697 284 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2922 3304 3590 1870
Size: 73 72 907 382 286 4545
Fragments arranged by size:
4545 907 382 286 73 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2705 2738 2705
Size: 33 6232
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3264 3359 3586 5041 618
Size: 325 217 627 1477 95 227 1455 1842
Fragments arranged by size:
1842 1477 1455 627 325 227 217 95
BanII G_rGCy'C
Cuts at: 1391 2016 2030 2189 2803 2935 3121 3324 3584
Size: 625 14 159 614 132 186 203 260
Cuts at: 3584 1391
Size: 4072
Fragments arranged by size:
4072 625 614 260 203 186 159 132
14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2759 928
Size: 971 860 4434
Fragments arranged by size:
4434 971 860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2684 4876 136
Size: 107 122 71 631 605 1012 2192 1525
Fragments arranged by size:
2192 1525 1012 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2030 2803 2935 3081 3267 3362 4326
Size: 226 14 773 132 146 186 95 964
Cuts at: 4326 5482 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 964 773 226 186 146 132
95 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2692 3395 3996
Size: 416 53 83 399 24 1275 703 601
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1275 958 703 601 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2546 2684 3057 4925 1239
Size: 804 503 138 373 1868 2579
Fragments arranged by size:
2579 1868 804 503 373 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4390 5255 5553 4390
Size: 865 298 5102
Fragments arranged by size:
5102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2436 4056 493
Size: 711 1232 1620 2702
Fragments arranged by size:
2702 1620 1232 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2918 3015 4072 4454
Size: 53 83 186 936 1385 97 1057 382
Cuts at: 4454 5089 275
Size: 635 1451
Fragments arranged by size:
1451 1385 1057 936 635 382 186 97
83 53
BsaWI w'CCGG_w
Cuts at: 3370 4698 5096 5370 5517 5928 3370
Size: 1328 398 274 147 411 3707
Fragments arranged by size:
3707 1328 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2056 2086 2299 2329 3326
Size: 30 165 30 653 30 213 30 997
Cuts at: 3326 3356 1178
Size: 30 4087
Fragments arranged by size:
4087 997 653 213 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2999 3002 3011 3111 3133 3181 3331
Size: 1240 573 3 9 100 22 48 150
Cuts at: 3331 3461 3470 1186
Size: 130 9 3981
Fragments arranged by size:
3981 1240 573 150 130 100 48 22
9 9 3
BseYI C'CCAG_C
Cuts at: 2022 2318 2846 2935 3171 3206 3429 3498 5468
Size: 296 528 89 236 35 223 69 1970
Cuts at: 5468 5964 2022
Size: 496 2323
Fragments arranged by size:
2323 1970 528 496 296 236 223 89
69 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2189 2400 3066 3324 3515 4326 4411 5482 2189
Size: 211 666 258 191 811 85 1071 2972
Fragments arranged by size:
2972 1071 811 666 258 211 191 85
BsmI GAATG_Cn'
Cuts at: 2486 3776 3869 2486
Size: 1290 93 4882
Fragments arranged by size:
4882 1290 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2030 2189 2400 2803 2935 3066
Size: 399 226 14 159 211 403 132 131
Cuts at: 3066 3081 3121 3267 3324 3362 3515 3584 4326
Size: 15 40 146 57 38 153 69 742
Cuts at: 4326 4411 5482 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 226 211 159
153 146 132 131 85 69 57 40
38 15 14
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2660 3620 844
Size: 1816 960 3489
Fragments arranged by size:
3489 1816 960
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 4152 1394
Size: 429 234 2095 3507
Fragments arranged by size:
3507 2095 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2377 4909 5096 1700
Size: 110 567 2532 187 2869
Fragments arranged by size:
2869 2532 567 187 110
BsrGI T'GTAC_A
Cuts at: 96 2280 96
Size: 2184 4081
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 3184 3184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2415 2467 3595 3962 4347 4364 5816 5902
Size: 443 52 1128 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1128 443 385 367 346 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 3444 3444
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 2004 2536 2795 513
Size: 552 939 532 259 3983
Fragments arranged by size:
3983 939 552 532 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2688 3501 487
Size: 683 56 1462 813 3251
Fragments arranged by size:
3251 1462 813 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3781 4575 4595 2584
Size: 1197 794 20 4254
Fragments arranged by size:
4254 1197 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DraIII CAC_nnn'GTG
Cuts at: 2830 2830
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 3558 3712
Size: 54 1425 50 275 18 709 1019 154
Cuts at: 3712 4601 6230 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1019 889 709 275 154 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2976 3312 3414 3447 4195 1353
Size: 861 762 336 102 33 748 3423
Fragments arranged by size:
3423 861 762 748 336 102 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2187 3322 2187
Size: 1135 5130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2098 2285 2492 2546 2684 3057
Size: 393 411 55 187 207 54 138 373
Cuts at: 3057 3144 3395 4328 4925 5712 5980 1239
Size: 87 251 933 597 787 268 1524
Fragments arranged by size:
1524 933 787 597 411 393 373 268
251 207 187 138 87 55 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2972 3199 2048
Size: 924 227 5114
Fragments arranged by size:
5114 924 227
EcoO109I rG'GnC_Cy
Cuts at: 2012 2026 2027 2067 2275 2668 2800 2931 2932
Size: 14 1 40 208 393 132 131 1
Cuts at: 2932 2965 3210 3395 2012
Size: 33 245 185 4882
Fragments arranged by size:
4882 393 245 208 185 132 131 40
33 14 1 1
EcoRI G'AATT_C
Cuts at: 3290 3574 3952 3290
Size: 284 378 5603
Fragments arranged by size:
5603 378 284
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2023 5412 1678
Size: 345 3389 2531
Fragments arranged by size:
3389 2531 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 2299 2331 3323
Size: 32 135 32 484 32 211 32 992
Cuts at: 3323 3355 4906 4938 4980 5012 1373
Size: 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 992 484 211 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 2299 6141 2299
Size: 3842 2423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2541 3560 10
Size: 54 1425 343 709 1019 2715
Fragments arranged by size:
2715 1425 1019 709 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4223 4582 4741 518
Size: 648 3057 359 159 2042
Fragments arranged by size:
3057 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2443 2570 2782 3498 3681 4358 5506 5751
Size: 837 127 212 716 183 677 1148 245
Cuts at: 5751 1606
Size: 2120
Fragments arranged by size:
2120 1148 837 716 677 245 212 183
127
NaeI GCC'GGC
Cuts at: 2379 2379
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2004 2536 513
Size: 552 939 532 4242
Fragments arranged by size:
4242 939 552 532
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2377 2377
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2458 3623 5168 1132
Size: 97 1229 1165 1545 2229
Fragments arranged by size:
2229 1545 1229 1165 97
PasI CC'CwG_GG
Cuts at: 1348 2138 2334 3114 3389 1348
Size: 790 196 780 275 4224
Fragments arranged by size:
4224 790 780 275 196
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2144 1108
Size: 1036 5229
PfoI T'CCnGG_A
Cuts at: 3312 3312
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2668 2965 3210 3395 2275
Size: 393 297 245 185 5145
Fragments arranged by size:
5145 393 297 245 185
PshAI GACnn'nnGTC
Cuts at: 1613 2857 1613
Size: 1244 5021
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2012 2026 2799 2931 2012
Size: 14 773 132 5346
Fragments arranged by size:
5346 773 132 14
PspXI vC'TCGA_Gb
Cuts at: 2922 2922
Size: 6265
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 6233
PstI C_TGCA'G
Cuts at: 830 2052 3617 830
Size: 1222 1565 3478
Fragments arranged by size:
3478 1565 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2443 2570 3498 2443
Size: 127 928 5210
Fragments arranged by size:
5210 928 127
SacI G_AGCT'C
Cuts at: 2189 3324 2189
Size: 1135 5130
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SanDI GG'GwC_CC
Cuts at: 3395 3395
Size: 6265
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2048 3613 4748 5429 5620 826
Size: 219 1003 1565 1135 681 191 1471
Fragments arranged by size:
1565 1471 1135 1003 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2922 4369 5270 5532 820
Size: 197 926 979 1447 901 262 1553
Fragments arranged by size:
1553 1447 979 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 2458 3623 1132
Size: 1326 1165 3774
Fragments arranged by size:
3774 1326 1165
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 2536 3244 3297 3704 513
Size: 552 939 231 301 708 53 407 3074
Fragments arranged by size:
3074 939 708 552 407 301 231 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 2417 2957 3530 3539 4292 4309
Size: 1255 746 91 540 573 9 753 17
Cuts at: 4309 4462 4647 5092 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 753 746 573 540 445 185
153 91 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 4511 6223
Size: 274 80 33 51 1443 303 2231 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
2231 1712 1443 303 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3033 3065 6225 6257 3033
Size: 32 3160 32 3041
Fragments arranged by size:
3160 3041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2679 3121 3251 2679
Size: 442 130 5693
Fragments arranged by size:
5693 442 130
XhoI C'TCGA_G
Cuts at: 1943 2922 1943
Size: 979 5286
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI RsrII SacII SexAI
SfiI SfoI SrfI SwaI