Restriction map for
pFN21AF0162
(Circular) (Six-base) MAPSORT of: pFN21AF0162.seq Check: 9623 from: 1 to: 6820
pFN21AF0162 6820 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3267 4163 1937
Size: 1330 896 4594
Fragments arranged by size:
4594 1330 896
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2025 2579 3863 4883 6267 6535 1632
Size: 393 554 1284 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1284 1020 554 393 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 3111 3151 5719 6696 1203
Size: 1908 40 2568 977 1327
Fragments arranged by size:
2568 1908 1327 977 40
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 3754 5549 3754
Size: 1795 5025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2457 1184
Size: 1273 5547
AlwNI CAG_nnn'CTG
Cuts at: 4563 6135 4563
Size: 1572 5248
ApaI G_GGCC'C
Cuts at: 3405 3405
Size: 6820
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 4129 4355 4507 6616 1493
Size: 2636 226 152 2109 1697
Fragments arranged by size:
2636 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 4145 1870
Size: 73 2202 4545
Fragments arranged by size:
4545 2202 73
BamHI G'GATC_C
Cuts at: 4150 4517 4150
Size: 367 6453
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3105 3971 4141 5596 618
Size: 325 217 627 1318 866 170 1455 1842
Fragments arranged by size:
1842 1455 1318 866 627 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2915 3122 3405 3495 3654 3792 4139 1391
Size: 1524 207 283 90 159 138 347 4072
Fragments arranged by size:
4072 1524 347 283 207 159 138 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3557 928
Size: 971 1658 4191
Fragments arranged by size:
4191 1658 971
BbvCI CC'TCA_GC
Cuts at: 3701 3701
Size: 6820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3995 4029 5011 5045 1903
Size: 34 2058 34 982 34 3678
Fragments arranged by size:
3678 2058 982 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4712 5922 3368
Size: 1344 1210 4266
Fragments arranged by size:
4266 1344 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6820
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3854 5431 136
Size: 107 122 71 631 605 2182 1577 1525
Fragments arranged by size:
2182 1577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3219 3981 6815 3219
Size: 762 2834 3224
Fragments arranged by size:
3224 2834 762
BlpI GC'TnA_GC
Cuts at: 2219 4237 2219
Size: 2018 4802
Bme1580I G_kGCm'C
Cuts at: 1790 2850 3405 4881 6037 1790
Size: 1060 555 1476 1156 2573
Fragments arranged by size:
2573 1476 1156 1060 555
BmgBI CAC'GTC
Cuts at: 1318 2605 3152 4045 1318
Size: 1287 547 893 4093
Fragments arranged by size:
4093 1287 893 547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 3128 3541
Size: 416 53 83 399 24 1137 574 413
Cuts at: 3541 4551 5509 442
Size: 1010 958 1753
Fragments arranged by size:
1753 1137 1010 958 574 416 413 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2486 2155
Size: 331 6489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2332 2899 3326 3650 3791 3974 5480
Size: 929 164 567 427 324 141 183 1506
Cuts at: 5480 1239
Size: 2579
Fragments arranged by size:
2579 1506 929 567 427 324 183 164
141
Bpu10I CC'TnA_GC
Cuts at: 3701 3701
Size: 6820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2463 4945 5810 6108 2463
Size: 2482 865 298 3175
Fragments arranged by size:
3175 2482 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 5483 882
Size: 617 525 3459 2219
Fragments arranged by size:
3459 2219 617 525
BsaAI yAC'GTr
Cuts at: 493 1204 4611 493
Size: 711 3407 2702
Fragments arranged by size:
3407 2702 711
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2763 4627 5009 5644
Size: 53 83 186 936 1230 1864 382 635
Cuts at: 5644 275
Size: 1451
Fragments arranged by size:
1864 1451 1230 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2103 3213 5253 5651 5925 6072 6483 2103
Size: 1110 2040 398 274 147 411 2440
Fragments arranged by size:
2440 2040 1110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6595
Fragments arranged by size:
6595 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2165 2223 2350 3059 3689 1186
Size: 856 123 58 127 709 630 4317
Fragments arranged by size:
4317 856 709 630 127 123 58
BseYI C'CCAG_C
Cuts at: 2681 3720 6023 6519 2681
Size: 1039 2303 496 2982
Fragments arranged by size:
2982 2303 1039 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 5566
Fragments arranged by size:
5566 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4270 5031 6059 1540
Size: 275 186 2269 761 1028 2301
Fragments arranged by size:
2301 2269 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2661 2679 3495 3792 4881 4966 6037 2661
Size: 18 816 297 1089 85 1071 3444
Fragments arranged by size:
3444 1089 1071 816 297 85 18
BsmI GAATG_Cn'
Cuts at: 4331 4424 4331
Size: 93 6727
BsmBI CGTCTCn'nnnn_
Cuts at: 2344 2344
Size: 6820
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2661 2679 2850 2915 3122 3405 3495
Size: 399 871 18 171 65 207 283 90
Cuts at: 3495 3654 3792 4139 4881 4966 6037 1391
Size: 159 138 347 742 85 1071 2174
Fragments arranged by size:
2174 1071 871 742 399 347 283 207
171 159 138 90 85 65 18
BspEI T'CCGG_A
Cuts at: 2103 3213 6483 2103
Size: 1110 3270 2440
Fragments arranged by size:
3270 2440 1110
BspHI T'CATG_A
Cuts at: 2895 4709 6439 2895
Size: 1814 1730 3276
Fragments arranged by size:
3276 1814 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4175 844
Size: 3331 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3569 4707 1394
Size: 429 1746 1138 3507
Fragments arranged by size:
3507 1746 1138 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4011 5315 5489 65
Size: 1183 2763 1304 174 1396
Fragments arranged by size:
2763 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3794 3829 5464 5651 1700
Size: 110 1984 35 1635 187 2869
Fragments arranged by size:
2869 1984 1635 187 110 35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2983 3339 794
Size: 2189 356 4275
Fragments arranged by size:
4275 2189 356
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstXI CCAn_nnnn'nTGG
Cuts at: 2386 2813 3141 2386
Size: 427 328 6065
Fragments arranged by size:
6065 427 328
BstYI r'GATC_y
Cuts at: 1972 2838 3219 3981 4150 4517 4902 4919 6371
Size: 866 381 762 169 367 385 17 1452
Cuts at: 6371 6457 6469 6815 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 866 762 385 381 367 346
169 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3966 3966
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2004 2852 2928 3160 3604 3636 3672
Size: 552 939 848 76 232 444 32 36
Cuts at: 3672 513
Size: 3661
Fragments arranged by size:
3661 939 848 552 444 232 76 36
32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6081
Fragments arranged by size:
6081 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 2134 2257 4336 5130 5150 2125
Size: 9 123 2079 794 20 3795
Fragments arranged by size:
3795 2079 794 123 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DraIII CAC_nnn'GTG
Cuts at: 3136 3152 3136
Size: 16 6804
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2528 5827 809
Size: 507 1212 3299 1802
Fragments arranged by size:
3299 1802 1212 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3462 3669 3819
Size: 54 1425 50 275 18 1632 207 150
Cuts at: 3819 3831 4267 5156 6785 8
Size: 12 436 889 1629 43
Fragments arranged by size:
1632 1629 1425 889 436 275 207 150
54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4267 1537
Size: 275 2455 4090
Fragments arranged by size:
4090 2455 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 3251 3448 4026 4750 1353
Size: 699 1199 197 578 724 3423
Fragments arranged by size:
3423 1199 724 699 578 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5407 5781 5927 387
Size: 1064 138 3818 374 146 1280
Fragments arranged by size:
3818 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3493 3790 3493
Size: 297 6523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2168 2332 2579 2899 3326 3650
Size: 393 393 143 164 247 320 427 324
Cuts at: 3650 3791 3863 3974 4883 5480 6267 6535 1239
Size: 141 72 111 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 597 427 393 393 324
320 268 247 164 143 141 111 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2435 3074 3929 3953 2347
Size: 88 639 855 24 5214
Fragments arranged by size:
5214 855 639 88 24
EcoO109I rG'GnC_Cy
Cuts at: 3401 3402 3401
Size: 1 6819
EcoRI G'AATT_C
Cuts at: 4129 4507 4129
Size: 378 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 6820
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 5967 1678
Size: 4289 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 3740 3772 4034
Size: 32 135 32 449 32 1687 32 262
Cuts at: 4034 4066 5461 5493 5535 5567 1373
Size: 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1687 1395 449 262 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4164 4410 669
Size: 988 281 2226 246 3079
Fragments arranged by size:
3079 2226 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 4410 4410
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 3969 5934 6118 1305
Size: 1083 1581 1965 184 2007
Fragments arranged by size:
2007 1965 1581 1083 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3671 10
Size: 54 1425 343 1839 3159
Fragments arranged by size:
3159 1839 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3026 3032 3170 3341 3665 4016
Size: 648 1779 81 6 138 171 324 351
Cuts at: 4016 4778 5137 5296 518
Size: 762 359 159 2042
Fragments arranged by size:
2042 1779 762 648 359 351 324 171
159 138 81 6
MspA1I CmG'CkG
Cuts at: 1606 2209 2267 2494 2592 3841 4236 4913 6061
Size: 603 58 227 98 1249 395 677 1148
Cuts at: 6061 6306 1606
Size: 245 2120
Fragments arranged by size:
2120 1249 1148 677 603 395 245 227
98 58
NcoI C'CATG_G
Cuts at: 513 1065 2004 3604 513
Size: 552 939 1600 3729
Fragments arranged by size:
3729 1600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 2986 3115 4178 5723 1132
Size: 97 1757 129 1063 1545 2229
Fragments arranged by size:
2229 1757 1545 1063 129 97
PasI CC'CwG_GG
Cuts at: 1348 2225 1348
Size: 877 5943
PciI A'CATG_T
Cuts at: 3111 5719 3111
Size: 2608 4212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2817 3136 3605 3745 1108
Size: 1709 319 469 140 4183
Fragments arranged by size:
4183 1709 469 319 140
PfoI T'CCnGG_A
Cuts at: 2348 2915 3366 2348
Size: 567 451 5802
Fragments arranged by size:
5802 567 451
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3934 1613
Size: 2321 4499
PsiI TTA'TAA
Cuts at: 2112 4390 2112
Size: 2278 4542
PspOMI G'GGCC_C
Cuts at: 3401 3401
Size: 6820
PstI C_TGCA'G
Cuts at: 830 2336 4172 830
Size: 1506 1836 3478
Fragments arranged by size:
3478 1836 1506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 2267 2494 2592 2267
Size: 227 98 6495
Fragments arranged by size:
6495 227 98
SacI G_AGCT'C
Cuts at: 3495 3792 3495
Size: 297 6523
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SapI GCTCTTCn'nnn_
Cuts at: 4026 4026
Size: 6820
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 5068 6780 5068
Size: 1712 5108
SfcI C'TryA_G
Cuts at: 826 1045 2332 4168 5303 5984 6175 826
Size: 219 1287 1836 1135 681 191 1471
Fragments arranged by size:
1836 1471 1287 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3854 3854
Size: 6820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 4147 1872
Size: 2275 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2478 4924 5825 6087 820
Size: 197 926 535 2446 901 262 1553
Fragments arranged by size:
2446 1553 926 901 535 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 2986 4178 1132
Size: 1854 1192 3774
Fragments arranged by size:
3774 1854 1192
SspI AAT'ATT
Cuts at: 5 52 4744 5
Size: 47 4692 2081
Fragments arranged by size:
4692 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6820
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 3604 4259 513
Size: 552 939 237 1363 655 3074
Fragments arranged by size:
3074 1363 939 655 552 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3147 3733 4847 4864 5017 5202 5647
Size: 1255 1567 586 1114 17 153 185 445
Cuts at: 5647 325
Size: 1498
Fragments arranged by size:
1567 1498 1255 1114 586 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2691 5066 6778
Size: 274 80 33 51 1443 714 2375 1712
Cuts at: 6778 96
Size: 138
Fragments arranged by size:
2375 1712 1443 714 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 6780 6812 2844
Size: 32 3904 32 2852
Fragments arranged by size:
3904 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3556 1316
Size: 2240 4580
XbaI T'CTAG_A
Cuts at: 4039 4156 4039
Size: 117 6703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3415 3415
Size: 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 4145 1870
Size: 2275 4545
XmnI GAAnn'nnTTC
Cuts at: 4949 4949
Size: 6820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI