Restriction map for
pFN21AF0137
(Circular) (Six-base) MAPSORT of: pFN21AF0137.seq Check: 9593 from: 1 to: 9229
pFN21AF0137 9229 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5599 5599
Size: 9229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7039 278
Size: 53 83 186 936 5503 2468
Fragments arranged by size:
5503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4054 5344 6572 1937
Size: 2117 1290 1228 4594
Fragments arranged by size:
4594 2117 1290 1228
Acc65I G'GTAC_C
Cuts at: 6550 6550
Size: 9229
AclI AA'CG_TT
Cuts at: 1563 7356 7729 1563
Size: 5793 373 3063
Fragments arranged by size:
5793 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2646 2834 2959 3866 5090 5491 5994 6003
Size: 1014 188 125 907 1224 401 503 9
Cuts at: 6003 6150 6458 7292 8676 8944 1632
Size: 147 308 834 1384 268 1917
Fragments arranged by size:
1917 1384 1224 1014 907 834 503 401
308 268 188 147 125 9
AfeI AGC'GCT
Cuts at: 3297 3297
Size: 9229
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9032
AflIII A'CryG_T
Cuts at: 1203 8128 9105 1203
Size: 6925 977 1327
Fragments arranged by size:
6925 1327 977
AgeI A'CCGG_T
Cuts at: 8060 8060
Size: 9229
AhdI GACnn_n'nnGTC
Cuts at: 7958 7958
Size: 9229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 2307
Size: 32 9197
AlwNI CAG_nnn'CTG
Cuts at: 4319 4871 5551 5605 5907 6972 8544 4319
Size: 552 680 54 302 1065 1572 5004
Fragments arranged by size:
5004 1572 1065 680 552 302 54
ApaI G_GGCC'C
Cuts at: 2132 2132
Size: 9229
ApaLI G'TGCA_C
Cuts at: 3836 4124 7286 8442 3836
Size: 288 3162 1156 4623
Fragments arranged by size:
4623 3162 1156 288
ApoI r'AATT_y
Cuts at: 1493 3262 5864 5896 6344 6538 6764 6916 9025
Size: 1769 2602 32 448 194 226 152 2109
Cuts at: 9025 1493
Size: 1697
Fragments arranged by size:
2602 2109 1769 1697 448 226 194 152
32
AseI AT'TA_AT
Cuts at: 160 1032 4464 7783 160
Size: 872 3432 3319 1606
Fragments arranged by size:
3432 3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9229
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2131 2361 6554 1870
Size: 73 132 56 230 4193 4545
Fragments arranged by size:
4545 4193 230 132 73 56
BamHI G'GATC_C
Cuts at: 4084 6559 6926 4084
Size: 2475 367 6387
Fragments arranged by size:
6387 2475 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3306 4861 5456 6550 8005
Size: 325 217 627 1519 1555 595 1094 1455
Cuts at: 8005 618
Size: 1842
Fragments arranged by size:
1842 1555 1519 1455 1094 627 595 325
217
BanII G_rGCy'C
Cuts at: 1391 2132 3897 3950 4026 5317 6081 6471 6548
Size: 741 1765 53 76 1291 764 390 77
Cuts at: 6548 1391
Size: 4072
Fragments arranged by size:
4072 1765 1291 764 741 390 77 76
53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 3422 3854 6511 928
Size: 971 205 1318 432 2657 3646
Fragments arranged by size:
3646 2657 1318 971 432 205
BbvCI CC'TCA_GC
Cuts at: 5084 5084
Size: 9229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7420 7454 1903
Size: 34 5483 34 3678
Fragments arranged by size:
5483 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7121 8331 7121
Size: 1210 8019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 6013 7840
Size: 107 122 71 631 605 416 3925 1827
Cuts at: 7840 136
Size: 1525
Fragments arranged by size:
3925 1827 1525 631 605 416 122 107
71
BglII A'GATC_T
Cuts at: 4795 9224 4795
Size: 4429 4800
BlpI GC'TnA_GC
Cuts at: 3328 5642 6646 3328
Size: 2314 1004 5911
Fragments arranged by size:
5911 2314 1004
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2132 3840 4128 5281 5459 7290 8446
Size: 286 56 1708 288 1153 178 1831 1156
Cuts at: 8446 1790
Size: 2573
Fragments arranged by size:
2573 1831 1708 1156 1153 288 286 178
56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5057 5314 6960
Size: 416 53 83 399 24 3640 257 1646
Cuts at: 6960 7918 442
Size: 958 1753
Fragments arranged by size:
3640 1753 1646 958 416 399 257 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4554 4554
Size: 9229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2691 2785 3861 4377 4410 4615 4796 5396
Size: 1452 94 1076 516 33 205 181 600
Cuts at: 5396 5417 5866 6407 7889 1239
Size: 21 449 541 1482 2579
Fragments arranged by size:
2579 1482 1452 1076 600 541 516 449
205 181 94 33 21
Bpu10I CC'TnA_GC
Cuts at: 5084 5523 5084
Size: 439 8790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4152 4270 4982 6052 7354 8219 8517 4152
Size: 118 712 1070 1302 865 298 4864
Fragments arranged by size:
4864 1302 1070 865 712 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 2361 4080 5880 6134 7892 882
Size: 617 710 152 1719 1800 254 1758 2219
Fragments arranged by size:
2219 1800 1758 1719 710 617 254 152
BsaAI yAC'GTr
Cuts at: 493 1204 3835 7020 493
Size: 711 2631 3185 2702
Fragments arranged by size:
3185 2702 2631 711
BsaBI GATnn'nnATC
Cuts at: 9223 9223
Size: 9229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5854 7036 7418 8053
Size: 53 83 186 936 4321 1182 382 635
Cuts at: 8053 275
Size: 1451
Fragments arranged by size:
4321 1451 1182 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3900 5700 6220 7662 8060 8334 8481 8892 3900
Size: 1800 520 1442 398 274 147 411 4237
Fragments arranged by size:
4237 1800 1442 520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 2307 2337 3359
Size: 30 165 30 766 30 108 30 1022
Cuts at: 3359 3389 5369 5399 1178
Size: 30 1980 30 5008
Fragments arranged by size:
5008 1980 1022 766 165 108 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2349 2838 3483 3525 4155 4367 4601 5419
Size: 1163 489 645 42 630 212 234 818
Cuts at: 5419 5422 1186
Size: 3 4993
Fragments arranged by size:
4993 1163 818 645 630 489 234 212
42 3
BseYI C'CCAG_C
Cuts at: 2879 5088 8432 8928 2879
Size: 2209 3344 496 3180
Fragments arranged by size:
3344 3180 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3136 4405 5696 1163
Size: 573 1400 1269 1291 4696
Fragments arranged by size:
4696 1400 1291 1269 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6679 7440 8468 1540
Size: 275 186 4678 761 1028 2301
Fragments arranged by size:
4678 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3840 4128 6081 6471 7290 7375 8446 3840
Size: 288 1953 390 819 85 1071 4623
Fragments arranged by size:
4623 1953 1071 819 390 288 85
BsmI GAATG_Cn'
Cuts at: 2695 6063 6740 6833 2695
Size: 3368 677 93 5091
Fragments arranged by size:
5091 3368 677 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2950 2950
Size: 9229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2132 3840 3897 3950 4026 4128
Size: 399 286 56 1708 57 53 76 102
Cuts at: 4128 5281 5317 5459 6081 6471 6548 7290 7375
Size: 1153 36 142 622 390 77 742 85
Cuts at: 7375 8446 1391
Size: 1071 2174
Fragments arranged by size:
2174 1708 1153 1071 742 622 399 390
286 142 102 85 77 76 57 56
53 36
BspEI T'CCGG_A
Cuts at: 5700 8892 5700
Size: 3192 6037
BspHI T'CATG_A
Cuts at: 2491 3766 3955 7118 8848 2491
Size: 1275 189 3163 1730 2872
Fragments arranged by size:
3163 2872 1730 1275 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5599 6584 844
Size: 4755 985 3489
Fragments arranged by size:
4755 3489 985
BsrBI CCG'CTC
Cuts at: 1394 1823 7116 1394
Size: 429 5293 3507
Fragments arranged by size:
5293 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2939 3673 7724 7898 65
Size: 1183 1691 734 4051 174 1396
Fragments arranged by size:
4051 1691 1396 1183 734 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7873 8060 1700
Size: 110 6063 187 2869
Fragments arranged by size:
6063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3616 96
Size: 3520 5709
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9229
BssSI C'ACGA_G
Cuts at: 7289 8301 7289
Size: 1012 8217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9229
BstBI TT'CG_AA
Cuts at: 2758 8033 2758
Size: 5275 3954
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9229
BstXI CCAn_nnnn'nTGG
Cuts at: 5179 5395 6428 5179
Size: 216 1033 7980
Fragments arranged by size:
7980 1033 216
BstYI r'GATC_y
Cuts at: 1972 2938 4084 4577 4795 6559 6926 7311 7328
Size: 966 1146 493 218 1764 367 385 17
Cuts at: 7328 8780 8866 8878 9224 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1764 1452 1146 966 493 385 367
346 218 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4215 4435 4215
Size: 220 9009
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 513
Size: 552 939 14 7724
Fragments arranged by size:
7724 939 552 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2079 487
Size: 683 56 853 7637
Fragments arranged by size:
7637 853 683 56
BtsI GCAGTG_nn'
Cuts at: 5496 5643 5961 6508 6745 7539 7559 5496
Size: 147 318 547 237 794 20 7166
Fragments arranged by size:
7166 794 547 318 237 147 20
ClaI AT'CG_AT
Cuts at: 1554 4274 1554
Size: 2720 6509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9194
DraI TTT'AAA
Cuts at: 6533 6533
Size: 9229
DraIII CAC_nnn'GTG
Cuts at: 4975 4975
Size: 9229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4050 8236 809
Size: 507 2734 4186 1802
Fragments arranged by size:
4186 2734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3189 3921 4753
Size: 54 1425 50 275 18 1359 732 832
Cuts at: 4753 5113 5181 5295 6676 7565 9194 8
Size: 360 68 114 1381 889 1629 43
Fragments arranged by size:
1629 1425 1381 1359 889 832 732 360
275 114 68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6676 1537
Size: 275 4864 4090
Fragments arranged by size:
4864 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 2815 2978 3172 4014 4523 5492 7159
Size: 1457 5 163 194 842 509 969 1667
Cuts at: 7159 1353
Size: 3423
Fragments arranged by size:
3423 1667 1457 969 842 509 194 163
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7816 8190 8336 387
Size: 1064 138 6227 374 146 1280
Fragments arranged by size:
6227 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6079 6469 6079
Size: 390 8839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 2691 2785 2834 2959 3861 3866
Size: 393 1014 45 94 49 125 902 5
Cuts at: 3866 4377 4410 4615 4796 5090 5396 5417 5491
Size: 511 33 205 181 294 306 21 74
Cuts at: 5491 5866 5994 6003 6150 6407 6458 7292 7889
Size: 375 128 9 147 257 51 834 597
Cuts at: 7889 8676 8944 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1014 902 834 787 597 511 393
375 306 294 268 257 205 181 147
128 125 94 74 51 49 45 33
21 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 4167 4167
Size: 9229
EcoO109I rG'GnC_Cy
Cuts at: 2080 4316 5059 5465 6417 2080
Size: 2236 743 406 952 4892
Fragments arranged by size:
4892 2236 952 743 406
EcoRI G'AATT_C
Cuts at: 5864 6538 6916 5864
Size: 674 378 8177
Fragments arranged by size:
8177 674 378
EcoRV GAT'ATC
Cuts at: 2340 2340
Size: 9229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2873 4264 4607 5306 5521 6097 6488 2873
Size: 1391 343 699 215 576 391 5614
Fragments arranged by size:
5614 1391 699 576 391 343 215
FspI TGC'GCA
Cuts at: 7735 7735
Size: 9229
HaeII r_GCGC'y
Cuts at: 1678 2857 3299 5684 8376 1678
Size: 1179 442 2385 2692 2531
Fragments arranged by size:
2692 2531 2385 1179 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 3167 3199 3359
Size: 32 135 32 1052 32 511 32 160
Cuts at: 3359 3391 3937 3969 4562 4594 5186 5218 5237
Size: 32 546 32 593 32 592 32 19
Cuts at: 5237 5269 5369 5401 5786 5818 7870 7902 7944
Size: 32 100 32 385 32 2052 32 42
Cuts at: 7944 7976 1373
Size: 32 2626
Fragments arranged by size:
2626 2052 1052 593 592 546 511 385
160 135 100 42 32 32 32 32
32 32 32 32 32 32 32 32
32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 4055 6179 6306 6573 6819
Size: 988 281 927 1190 2124 127 267 246
Cuts at: 6819 669
Size: 3079
Fragments arranged by size:
3079 2124 1190 988 927 281 267 246
127
HindIII A'AGCT_T
Cuts at: 748 3545 3740 4902 748
Size: 2797 195 1162 5075
Fragments arranged by size:
5075 2797 1162 195
HpaI GTT'AAC
Cuts at: 6306 6819 6306
Size: 513 8716
KpnI G_GTAC'C
Cuts at: 6554 6554
Size: 9229
MfeI C'AATT_G
Cuts at: 6828 6828
Size: 9229
MluI A'CGCG_T
Cuts at: 9105 9105
Size: 9229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 3402 3614 3751 3840 6184 8343 8527
Size: 981 1116 212 137 89 2344 2159 184
Cuts at: 8527 1305
Size: 2007
Fragments arranged by size:
2344 2159 2007 1116 981 212 184 137
89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3191 4755 5115 5183 5297
Size: 54 1425 343 1359 1564 360 68 114
Cuts at: 5297 10
Size: 3942
Fragments arranged by size:
3942 1564 1425 1359 360 343 114 68
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2589 3771 3833 3957 4142 5729
Size: 648 1365 58 1182 62 124 185 1587
Cuts at: 5729 7187 7546 7705 518
Size: 1458 359 159 2042
Fragments arranged by size:
2042 1587 1458 1365 1182 648 359 185
159 124 62 58
MspA1I CmG'CkG
Cuts at: 1606 2020 2058 2899 3055 3120 3504 4397 4871
Size: 414 38 841 156 65 384 893 474
Cuts at: 4871 4918 5088 5332 6645 7322 8470 8715 1606
Size: 47 170 244 1313 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 893 841 677 474 414
384 245 244 170 156 65 47 38
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7738
Fragments arranged by size:
7738 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9229
NotI GC'GGCC_GC
Cuts at: 6676 6676
Size: 9229
NspI r_CATG'y
Cuts at: 1132 1229 2321 6098 6587 8132 1132
Size: 97 1092 3777 489 1545 2229
Fragments arranged by size:
3777 2229 1545 1092 489 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9229
PciI A'CATG_T
Cuts at: 8128 8128
Size: 9229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2610 3759 4871 5304 5653 5907 1108
Size: 1502 1149 1112 433 349 254 4430
Fragments arranged by size:
4430 1502 1149 1112 433 349 254
PfoI T'CCnGG_A
Cuts at: 4014 4014
Size: 9229
PmeI GTTT'AAAC
Cuts at: 6533 6533
Size: 9229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2307 2339 7298 7330 8811 8843 2307
Size: 32 4959 32 1481 32 2693
Fragments arranged by size:
4959 2693 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4316 5059 6417 4316
Size: 743 1358 7128
Fragments arranged by size:
7128 1358 743
PshAI GACnn'nnGTC
Cuts at: 1613 2108 1613
Size: 495 8734
PsiI TTA'TAA
Cuts at: 6799 6799
Size: 9229
PspOMI G'GGCC_C
Cuts at: 2128 2128
Size: 9229
PspXI vC'TCGA_Gb
Cuts at: 2361 2361
Size: 9229
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5549 5581 5549
Size: 32 9197
PstI C_TGCA'G
Cuts at: 830 2063 4822 5221 5331 5491 5556 6251 6581
Size: 1233 2759 399 110 160 65 695 330
Cuts at: 6581 830
Size: 3478
Fragments arranged by size:
3478 2759 1233 695 399 330 160 110
65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9229
PvuII CAG'CTG
Cuts at: 2899 3055 3120 3504 4871 4918 5088 5332 2899
Size: 156 65 384 1367 47 170 244 6796
Fragments arranged by size:
6796 1367 384 244 170 156 65 47
SacI G_AGCT'C
Cuts at: 6081 6471 6081
Size: 390 8839
SacII CC_GC'GG
Cuts at: 2021 2021
Size: 9229
SalI G'TCGA_C
Cuts at: 1936 4053 6571 1936
Size: 2117 2518 4594
Fragments arranged by size:
4594 2518 2117
SapI GCTCTTCn'nnn_
Cuts at: 4014 5492 4014
Size: 1478 7751
SbfI CC_TGCA'GG
Cuts at: 6581 6581
Size: 9229
ScaI AGT'ACT
Cuts at: 6067 7477 9189 6067
Size: 1410 1712 6107
Fragments arranged by size:
6107 1712 1410
SfcI C'TryA_G
Cuts at: 826 1045 2059 2694 4818 5124 5217 5327 5345
Size: 219 1014 635 2124 306 93 110 18
Cuts at: 5345 5487 5552 6247 6577 7712 8393 8584 826
Size: 142 65 695 330 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 1014 695 681 635 330
306 219 191 142 110 93 65 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9229
SmaI CCC'GGG
Cuts at: 1872 2077 2133 6556 1872
Size: 205 56 4423 4545
Fragments arranged by size:
4545 4423 205 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2361 4167 4249 4997 6031 7333
Size: 197 926 418 1806 82 748 1034 1302
Cuts at: 7333 8234 8496 820
Size: 901 262 1553
Fragments arranged by size:
1806 1553 1302 1034 926 901 748 418
262 197 82
SnaBI TAC'GTA
Cuts at: 493 7020 493
Size: 6527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9229
SphI G_CATG'C
Cuts at: 1132 6098 6587 1132
Size: 4966 489 3774
Fragments arranged by size:
4966 3774 489
SrfI GCCC'GGGC
Cuts at: 2133 2133
Size: 9229
SspI AAT'ATT
Cuts at: 5 52 3690 7153 5
Size: 47 3638 3463 2081
Fragments arranged by size:
3638 3463 2081 47
StuI AGG'CCT
Cuts at: 1517 5837 6016 6365 1517
Size: 4320 179 349 4381
Fragments arranged by size:
4381 4320 349 179
StyI C'CwwG_G
Cuts at: 513 1065 2004 3108 3136 3285 3315 5037 6144
Size: 552 939 1104 28 149 30 1722 1107
Cuts at: 6144 6668 513
Size: 524 3074
Fragments arranged by size:
3074 1722 1107 1104 939 552 524 149
30 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3324 5296 7256 7273 7426 7611 8056
Size: 1255 1744 1972 1960 17 153 185 445
Cuts at: 8056 325
Size: 1498
Fragments arranged by size:
1972 1960 1744 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 3616 3719
Size: 274 80 33 51 1443 261 1378 103
Cuts at: 3719 4559 5742 6065 7475 9187 96
Size: 840 1183 323 1410 1712 138
Fragments arranged by size:
1712 1443 1410 1378 1183 840 323 274
261 138 103 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2169 2201 3535 3567 4946 4978 9189 9221 2169
Size: 32 1334 32 1379 32 4211 32 2177
Fragments arranged by size:
4211 2177 1379 1334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5063 1316
Size: 3747 5482
XbaI T'CTAG_A
Cuts at: 2774 4403 6565 2774
Size: 1629 2162 5438
Fragments arranged by size:
5438 2162 1629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5305 5305
Size: 9229
XhoI C'TCGA_G
Cuts at: 1943 2361 1943
Size: 418 8811
XmaI C'CCGG_G
Cuts at: 1870 2075 2131 6554 1870
Size: 205 56 4423 4545
Fragments arranged by size:
4545 4423 205 56
XmnI GAAnn'nnTTC
Cuts at: 2565 3690 4674 5541 7358 2565
Size: 1125 984 867 1817 4436
Fragments arranged by size:
4436 1817 1125 984 867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7037 276
Size: 53 83 186 936 5503 2468
Fragments arranged by size:
5503 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI RsrII SanDI SexAI SfiI SfoI SwaI