Restriction map for
pFN21AF0107
(Circular) (Six-base) MAPSORT of: pFN21AF0107.seq Check: 6158 from: 1 to: 8638
pFN21AF0107 8638 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2899 3892 4149 2899
Size: 993 257 7388
Fragments arranged by size:
7388 993 257
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6448 278
Size: 53 83 186 936 4912 2468
Fragments arranged by size:
4912 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 3432 5981 1937
Size: 919 576 2549 4594
Fragments arranged by size:
4594 2549 919 576
Acc65I G'GTAC_C
Cuts at: 5959 5959
Size: 8638
AclI AA'CG_TT
Cuts at: 1563 5436 6765 7138 1563
Size: 3873 1329 373 3063
Fragments arranged by size:
3873 3063 1329 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 3464 4064 4115 5432 6701 8085 8353
Size: 462 1370 600 51 1317 1269 1384 268
Cuts at: 8353 1632
Size: 1917
Fragments arranged by size:
1917 1384 1370 1317 1269 600 462 268
51
AflII C'TTAA_G
Cuts at: 820 1017 3371 3814 3854 820
Size: 197 2354 443 40 5604
Fragments arranged by size:
5604 2354 443 197 40
AflIII A'CryG_T
Cuts at: 1203 2592 4803 4881 7537 8514 1203
Size: 1389 2211 78 2656 977 1327
Fragments arranged by size:
2656 2211 1389 1327 977 78
AgeI A'CCGG_T
Cuts at: 7469 7469
Size: 8638
AhdI GACnn_n'nnGTC
Cuts at: 7367 7367
Size: 8638
AleI CACnn'nnGTG
Cuts at: 1166 2148 2532 4118 4388 5934 1166
Size: 982 384 1586 270 1546 3870
Fragments arranged by size:
3870 1586 1546 982 384 270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5097 5129 5097
Size: 32 8606
AlwNI CAG_nnn'CTG
Cuts at: 6381 7953 6381
Size: 1572 7066
ApaLI G'TGCA_C
Cuts at: 2141 3879 4264 6695 7851 2141
Size: 1738 385 2431 1156 2928
Fragments arranged by size:
2928 2431 1738 1156 385
ApoI r'AATT_y
Cuts at: 1493 3703 4019 5047 5222 5947 6173 6325 8434
Size: 2210 316 1028 175 725 226 152 2109
Cuts at: 8434 1493
Size: 1697
Fragments arranged by size:
2210 2109 1697 1028 725 316 226 175
152
AseI AT'TA_AT
Cuts at: 160 1032 2205 7192 160
Size: 872 1173 4987 1606
Fragments arranged by size:
4987 1606 1173 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
AvaI C'yCGr_G
Cuts at: 1870 1943 3193 5963 1870
Size: 73 1250 2770 4545
Fragments arranged by size:
4545 2770 1250 73
AvrII C'CTAG_G
Cuts at: 4861 4861
Size: 8638
BamHI G'GATC_C
Cuts at: 5968 6335 5968
Size: 367 8271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4382 5765 5959 7414 618
Size: 325 217 627 2595 1383 194 1455 1842
Fragments arranged by size:
2595 1842 1455 1383 627 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2871 4779 5496 5957 1391
Size: 1480 1908 717 461 4072
Fragments arranged by size:
4072 1908 1480 717 461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2118 2136 2787 4156 4641 5420
Size: 971 171 48 18 651 1369 485 779
Cuts at: 5420 5588 5856 928
Size: 168 268 3710
Fragments arranged by size:
3710 1369 971 779 651 485 268 171
168 48 18
BbvCI CC'TCA_GC
Cuts at: 2393 2393
Size: 8638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6829 6863 1903
Size: 34 4892 34 3678
Fragments arranged by size:
4892 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6530 7740 6530
Size: 1210 7428
BclI T'GATC_A
Cuts at: 1548 2563 4068 1548
Size: 1015 1505 6118
Fragments arranged by size:
6118 1505 1015
BfrBI ATG'CAT
Cuts at: 3539 5595 3539
Size: 2056 6582
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7249 136
Size: 107 122 71 631 605 5577 1525
Fragments arranged by size:
5577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5239 5856 8633 5239
Size: 617 2777 5244
Fragments arranged by size:
5244 2777 617
BlpI GC'TnA_GC
Cuts at: 2180 4773 6055 2180
Size: 2593 1282 4763
Fragments arranged by size:
4763 2593 1282
Bme1580I G_kGCm'C
Cuts at: 1790 2145 3883 4268 4889 6699 7855 1790
Size: 355 1738 385 621 1810 1156 2573
Fragments arranged by size:
2573 1810 1738 1156 621 385 355
BmgBI CAC'GTC
Cuts at: 1318 2595 3890 5185 1318
Size: 1277 1295 1295 4771
Fragments arranged by size:
4771 1295 1295 1277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4288 5560 6369
Size: 416 53 83 399 24 2871 1272 809
Cuts at: 6369 7327 442
Size: 958 1753
Fragments arranged by size:
2871 1753 1272 958 809 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2902 3180 2861
Size: 41 278 8319
Fragments arranged by size:
8319 278 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2831 3063 3627 3918 4764 4979 5447
Size: 908 684 232 564 291 846 215 468
Cuts at: 5447 5708 7298 1239
Size: 261 1590 2579
Fragments arranged by size:
2579 1590 908 846 684 564 468 291
261 232 215
Bpu10I CC'TnA_GC
Cuts at: 2393 3390 2393
Size: 997 7641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5062 6763 7628 7926 5062
Size: 1701 865 298 5774
Fragments arranged by size:
5774 1701 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2876 3645 4719 7301 882
Size: 617 1377 769 1074 2582 2219
Fragments arranged by size:
2582 2219 1377 1074 769 617
BsaAI yAC'GTr
Cuts at: 493 1204 6429 493
Size: 711 5225 2702
Fragments arranged by size:
5225 2702 711
BsaBI GATnn'nnATC
Cuts at: 8632 8632
Size: 8638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6445 6827 7462 275
Size: 53 83 186 936 4912 382 635 1451
Fragments arranged by size:
4912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2702 3007 7071 7469 7743 7890 8301 2702
Size: 305 4064 398 274 147 411 3039
Fragments arranged by size:
4064 3039 411 398 305 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 1178
Size: 30 165 30 817 30 7566
Fragments arranged by size:
7566 817 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2894 5021 1186
Size: 949 759 2127 4803
Fragments arranged by size:
4803 2127 949 759
BseYI C'CCAG_C
Cuts at: 7841 8337 7841
Size: 496 8142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2767 3870 4159 4577 1163
Size: 573 1031 1103 289 418 5224
Fragments arranged by size:
5224 1103 1031 573 418 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6088 6849 7877 1540
Size: 275 186 4087 761 1028 2301
Fragments arranged by size:
4087 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2871 3883 4268 4779 4811 5496 5745 6699
Size: 726 1012 385 511 32 685 249 954
Cuts at: 6699 6784 7855 2145
Size: 85 1071 2928
Fragments arranged by size:
2928 1071 1012 954 726 685 511 385
249 85 32
BsmI GAATG_Cn'
Cuts at: 2822 3539 5687 6149 6242 2822
Size: 717 2148 462 93 5218
Fragments arranged by size:
5218 2148 717 462 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2871 3883 4268 4779 4811 4889
Size: 399 355 726 1012 385 511 32 78
Cuts at: 4889 5496 5745 5957 6699 6784 7855 1391
Size: 607 249 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 1012 742 726 607 511 399
385 355 249 212 85 78 32
BspEI T'CCGG_A
Cuts at: 8301 8301
Size: 8638
BspHI T'CATG_A
Cuts at: 2674 2892 6527 8257 2674
Size: 218 3635 1730 3055
Fragments arranged by size:
3635 3055 1730 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2659 2899 3892 4149 5993 844
Size: 1815 240 993 257 1844 3489
Fragments arranged by size:
3489 1844 1815 993 257 240
BsrBI CCG'CTC
Cuts at: 1394 1823 2840 3164 4903 6525 1394
Size: 429 1017 324 1739 1622 3507
Fragments arranged by size:
3507 1739 1622 1017 429 324
BsrDI GCAATG_nn'
Cuts at: 65 1248 2796 3553 4406 5020 7133 7307 65
Size: 1183 1548 757 853 614 2113 174 1396
Fragments arranged by size:
2113 1548 1396 1183 853 757 614 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5285 7282 7469 1700
Size: 110 3475 1997 187 2869
Fragments arranged by size:
3475 2869 1997 187 110
BsrGI T'GTAC_A
Cuts at: 96 2979 3698 5867 96
Size: 2883 719 2169 2867
Fragments arranged by size:
2883 2867 2169 719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8638
BssSI C'ACGA_G
Cuts at: 2864 5495 6698 7710 2864
Size: 2631 1203 1012 3792
Fragments arranged by size:
3792 2631 1203 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2454 3518 4182 4272 4365 4585 4806 5691
Size: 1660 1064 664 90 93 220 221 885
Cuts at: 5691 794
Size: 3741
Fragments arranged by size:
3741 1660 1064 885 664 221 220 93
90
BstBI TT'CG_AA
Cuts at: 4509 5751 7442 4509
Size: 1242 1691 5705
Fragments arranged by size:
5705 1691 1242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8638
BstYI r'GATC_y
Cuts at: 1972 2033 2574 5239 5856 5968 6335 6720 6737
Size: 61 541 2665 617 112 367 385 17
Cuts at: 6737 8189 8275 8287 8633 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2665 1977 1452 617 541 385 367 346
112 86 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2305 2629 3111 5116 5839 513
Size: 552 1240 324 482 2005 723 3312
Fragments arranged by size:
3312 2005 1240 723 552 482 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7899
Fragments arranged by size:
7899 683 56
BtsI GCAGTG_nn'
Cuts at: 2527 2539 5941 6154 6948 6968 2527
Size: 12 3402 213 794 20 4197
Fragments arranged by size:
4197 3402 794 213 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3096 3131 529
Size: 35 2532 35 6036
Fragments arranged by size:
6036 2532 35 35
DraI TTT'AAA
Cuts at: 5942 5942
Size: 8638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2593 4205 7645 809
Size: 507 1277 1612 3440 1802
Fragments arranged by size:
3440 1802 1612 1277 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2261 3834 6085
Size: 54 1425 50 275 18 431 1573 2251
Cuts at: 6085 6974 8603 8
Size: 889 1629 43
Fragments arranged by size:
2251 1629 1573 1425 889 431 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6085 1537
Size: 275 4273 4090
Fragments arranged by size:
4273 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2876 3149 3483 3585 3586 4998 6568
Size: 743 780 273 334 102 1 1412 1570
Cuts at: 6568 1353
Size: 3423
Fragments arranged by size:
3423 1570 1412 780 743 334 273 102
1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7225 7599 7745 387
Size: 1064 138 5636 374 146 1280
Fragments arranged by size:
5636 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 4777 5494 2869
Size: 1908 717 6013
Fragments arranged by size:
6013 1908 717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2147 2831 3063 3464 3627 3918
Size: 393 462 53 684 232 401 163 291
Cuts at: 3918 4064 4115 4764 4979 5432 5447 5708 6701
Size: 146 51 649 215 453 15 261 993
Cuts at: 6701 7298 8085 8353 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 993 787 684 649 597 462 453
401 393 291 268 261 232 215 163
146 53 51 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4184 4184
Size: 8638
EcoO109I rG'GnC_Cy
Cuts at: 3337 3394 3810 4865 3337
Size: 57 416 1055 7110
Fragments arranged by size:
7110 1055 416 57
EcoRI G'AATT_C
Cuts at: 4019 5047 5222 5947 6325 4019
Size: 1028 175 725 378 6332
Fragments arranged by size:
6332 1028 725 378 175
EcoRV GAT'ATC
Cuts at: 2188 5257 2188
Size: 3069 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 5908 2596
Size: 3312 5326
FspI TGC'GCA
Cuts at: 5206 7144 5206
Size: 1938 6700
FspAI rTGC'GCAy
Cuts at: 5206 5206
Size: 8638
HaeII r_GCGC'y
Cuts at: 1678 5313 7785 1678
Size: 3635 2472 2531
Fragments arranged by size:
3635 2531 2472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3833 3865 3891 3923 3988
Size: 32 135 32 2261 32 26 32 65
Cuts at: 3988 4020 7279 7311 7353 7385 1373
Size: 32 3259 32 42 32 2626
Fragments arranged by size:
3259 2626 2261 135 65 42 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 2216 3463 4642 5982 6228 669
Size: 988 281 278 1247 1179 1340 246 3079
Fragments arranged by size:
3079 1340 1247 1179 988 281 278 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8638
HpaI GTT'AAC
Cuts at: 6228 6228
Size: 8638
KpnI G_GTAC'C
Cuts at: 5963 5963
Size: 8638
MfeI C'AATT_G
Cuts at: 6237 6237
Size: 8638
MluI A'CGCG_T
Cuts at: 8514 8514
Size: 8638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2273 2622 3111 5550 7752 7936 1305
Size: 801 167 349 489 2439 2202 184 2007
Fragments arranged by size:
2439 2202 2007 801 489 349 184 167
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2263 3836 10
Size: 54 1425 343 431 1573 4812
Fragments arranged by size:
4812 1573 1425 431 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2359 2532 2611 4118 4388 5934
Size: 648 982 211 173 79 1507 270 1546
Cuts at: 5934 6596 6955 7114 518
Size: 662 359 159 2042
Fragments arranged by size:
2042 1546 1507 982 662 648 359 270
211 173 159 79
MspA1I CmG'CkG
Cuts at: 1606 2148 2157 3222 6054 6731 7879 8124 1606
Size: 542 9 1065 2832 677 1148 245 2120
Fragments arranged by size:
2832 2120 1148 1065 677 542 245 9
NaeI GCC'GGC
Cuts at: 5287 5287
Size: 8638
NcoI C'CATG_G
Cuts at: 513 1065 2305 3111 5116 5839 513
Size: 552 1240 806 2005 723 3312
Fragments arranged by size:
3312 2005 1240 806 723 552
NdeI CA'TA_TG
Cuts at: 387 4848 5272 387
Size: 4461 424 3753
Fragments arranged by size:
4461 3753 424
NgoMIV G'CCGG_C
Cuts at: 5285 5285
Size: 8638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8638
NotI GC'GGCC_GC
Cuts at: 6085 6085
Size: 8638
NsiI A_TGCA'T
Cuts at: 3541 5597 3541
Size: 2056 6582
NspI r_CATG'y
Cuts at: 1132 1229 2668 4052 4153 4885 5083 5134 5996
Size: 97 1439 1384 101 732 198 51 862
Cuts at: 5996 7541 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1439 1384 862 732 198 101
97 51
PasI CC'CwG_GG
Cuts at: 1348 2345 1348
Size: 997 7641
PciI A'CATG_T
Cuts at: 4881 7537 4881
Size: 2656 5982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3650 1108
Size: 2542 6096
PfoI T'CCnGG_A
Cuts at: 2708 2775 4839 2708
Size: 67 2064 6507
Fragments arranged by size:
6507 2064 67
PmeI GTTT'AAAC
Cuts at: 5942 5942
Size: 8638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6707 6739 8220 8252 6707
Size: 32 1481 32 7093
Fragments arranged by size:
7093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3337 3394 3337
Size: 57 8581
PshAI GACnn'nnGTC
Cuts at: 1613 3399 1613
Size: 1786 6852
PsiI TTA'TAA
Cuts at: 6208 6208
Size: 8638
PspXI vC'TCGA_Gb
Cuts at: 3193 3193
Size: 8638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3433 3465 3433
Size: 32 8606
PstI C_TGCA'G
Cuts at: 830 2459 2534 3270 3523 4581 4710 5158 5696
Size: 1629 75 736 253 1058 129 448 538
Cuts at: 5696 5910 5990 830
Size: 214 80 3478
Fragments arranged by size:
3478 1629 1058 736 538 448 253 214
129 80 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8638
PvuII CAG'CTG
Cuts at: 2148 2157 3222 2148
Size: 9 1065 7564
Fragments arranged by size:
7564 1065 9
SacI G_AGCT'C
Cuts at: 2871 4779 5496 2871
Size: 1908 717 6013
Fragments arranged by size:
6013 1908 717
SalI G'TCGA_C
Cuts at: 1936 5980 1936
Size: 4044 4594
SapI GCTCTTCn'nnn_
Cuts at: 2876 3585 2876
Size: 709 7929
SbfI CC_TGCA'GG
Cuts at: 5990 5990
Size: 8638
ScaI AGT'ACT
Cuts at: 6886 8598 6886
Size: 1712 6926
SfcI C'TryA_G
Cuts at: 826 1045 2455 2530 3217 3266 3519 4258 4444
Size: 219 1410 75 687 49 253 739 186
Cuts at: 4444 4577 4706 5154 5692 5906 5986 7121 7802
Size: 133 129 448 538 214 80 1135 681
Cuts at: 7802 7993 826
Size: 191 1471
Fragments arranged by size:
1471 1410 1135 739 687 681 538 448
253 219 214 191 186 133 129 80
75 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8638
SmaI CCC'GGG
Cuts at: 1872 5965 1872
Size: 4093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3193 3371 3814 3854 5041 6742
Size: 197 926 1250 178 443 40 1187 1701
Cuts at: 6742 7643 7905 820
Size: 901 262 1553
Fragments arranged by size:
1701 1553 1250 1187 926 901 443 262
197 178 40
SnaBI TAC'GTA
Cuts at: 493 6429 493
Size: 5936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8638
SphI G_CATG'C
Cuts at: 1132 5083 5996 1132
Size: 3951 913 3774
Fragments arranged by size:
3951 3774 913
SspI AAT'ATT
Cuts at: 5 52 3714 6562 5
Size: 47 3662 2848 2081
Fragments arranged by size:
3662 2848 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8638
StyI C'CwwG_G
Cuts at: 513 1065 2305 3111 3771 4339 4861 5100 5116
Size: 552 1240 806 660 568 522 239 16
Cuts at: 5116 5839 6077 513
Size: 723 238 3074
Fragments arranged by size:
3074 1240 806 723 660 568 552 522
239 238 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2850 4537 6665 6682 6835 7020 7465
Size: 1255 1270 1687 2128 17 153 185 445
Cuts at: 7465 325
Size: 1498
Fragments arranged by size:
2128 1687 1498 1270 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2979 3698 3930
Size: 274 80 33 51 1443 1002 719 232
Cuts at: 3930 4428 5867 6884 8596 96
Size: 498 1439 1017 1712 138
Fragments arranged by size:
1712 1443 1439 1017 1002 719 498 274
232 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3970 4002 8598 8630 3970
Size: 32 4596 32 3978
Fragments arranged by size:
4596 3978 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8638
XbaI T'CTAG_A
Cuts at: 5974 5974
Size: 8638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3094 5239 3094
Size: 2145 6493
XhoI C'TCGA_G
Cuts at: 1943 3193 1943
Size: 1250 7388
XmaI C'CCGG_G
Cuts at: 1870 5963 1870
Size: 4093 4545
XmnI GAAnn'nnTTC
Cuts at: 5888 6767 5888
Size: 879 7759
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6446 276
Size: 53 83 186 936 4912 2468
Fragments arranged by size:
4912 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII FseI KasI NarI NruI PacI
PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI