Restriction map for
pFN21AF0068
(Circular) (Six-base) MAPSORT of: pFN21AF0068.seq Check: 6880 from: 1 to: 8278
pFN21AF0068 8278 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3142 3406 4012 5085 3142
Size: 264 606 1073 6335
Fragments arranged by size:
6335 1073 606 264
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6088 278
Size: 53 83 186 936 4552 2468
Fragments arranged by size:
4552 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5621 1937
Size: 3684 4594
Acc65I G'GTAC_C
Cuts at: 5599 5599
Size: 8278
AclI AA'CG_TT
Cuts at: 1563 6405 6778 1563
Size: 4842 373 3063
Fragments arranged by size:
4842 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 2891 3236 3673 3965 4852 5234 6341
Size: 1013 246 345 437 292 887 382 1107
Cuts at: 6341 7725 7993 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1107 1013 887 437 382 345
292 268 246
AfeI AGC'GCT
Cuts at: 4919 4919
Size: 8278
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8081
AflIII A'CryG_T
Cuts at: 1203 7177 8154 1203
Size: 5974 977 1327
Fragments arranged by size:
5974 1327 977
AgeI A'CCGG_T
Cuts at: 3955 7109 3955
Size: 3154 5124
AhdI GACnn_n'nnGTC
Cuts at: 7007 7007
Size: 8278
AleI CACnn'nnGTG
Cuts at: 1166 5018 1166
Size: 3852 4426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 4660 1184
Size: 1245 2231 4802
Fragments arranged by size:
4802 2231 1245
AlwNI CAG_nnn'CTG
Cuts at: 2740 2848 2920 3496 3550 3658 3775 4124 4633
Size: 108 72 576 54 108 117 349 509
Cuts at: 4633 5526 5545 6021 7593 2740
Size: 893 19 476 1572 3425
Fragments arranged by size:
3425 1572 893 576 509 476 349 117
108 108 72 54 19
ApaI G_GGCC'C
Cuts at: 4655 4890 4655
Size: 235 8043
ApaLI G'TGCA_C
Cuts at: 4697 6335 7491 4697
Size: 1638 1156 5484
Fragments arranged by size:
5484 1638 1156
ApoI r'AATT_y
Cuts at: 1493 2210 2769 3623 5587 5813 5965 8074 1493
Size: 717 559 854 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 854 717 559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6832 160
Size: 872 5800 1606
Fragments arranged by size:
5800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8278
AvaI C'yCGr_G
Cuts at: 1870 1943 2604 3375 3754 4283 5235 5603 1870
Size: 73 661 771 379 529 952 368 4545
Fragments arranged by size:
4545 952 771 661 529 379 368 73
BamHI G'GATC_C
Cuts at: 2513 5608 5975 2513
Size: 3095 367 4816
Fragments arranged by size:
4816 3095 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3648 3795 3897 4133 4410
Size: 325 217 627 1861 147 102 236 277
Cuts at: 4410 4818 5599 7054 618
Size: 408 781 1455 1842
Fragments arranged by size:
1861 1842 1455 781 627 408 325 277
236 217 147 102
BanII G_rGCy'C
Cuts at: 1391 2432 2805 3248 3453 3613 3838 3857 4255
Size: 1041 373 443 205 160 225 19 398
Cuts at: 4255 4655 4668 4890 5597 1391
Size: 400 13 222 707 4072
Fragments arranged by size:
4072 1041 707 443 400 398 373 225
222 205 160 19 13
BbeI G_GCGC'C
Cuts at: 4822 4822
Size: 8278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7307
BbvCI CC'TCA_GC
Cuts at: 2342 3711 5427 5463 2342
Size: 1369 1716 36 5157
Fragments arranged by size:
5157 1716 1369 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2220 2254 6469 6503 1903
Size: 34 283 34 4215 34 3678
Fragments arranged by size:
4215 3678 283 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5403 6170 7380 5403
Size: 767 1210 6301
Fragments arranged by size:
6301 1210 767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 2887 3195
Size: 107 122 71 631 605 1159 56 308
Cuts at: 3195 6889 136
Size: 3694 1525
Fragments arranged by size:
3694 1525 1159 631 605 308 122 107
71 56
BglII A'GATC_T
Cuts at: 4983 5475 8273 4983
Size: 492 2798 4988
Fragments arranged by size:
4988 2798 492
BlpI GC'TnA_GC
Cuts at: 2804 5695 2804
Size: 2891 5387
Bme1580I G_kGCm'C
Cuts at: 1790 2261 2644 3052 3090 3592 4138 4308 4333
Size: 471 383 408 38 502 546 170 25
Cuts at: 4333 4524 4655 4701 4890 6339 7495 1790
Size: 191 131 46 189 1449 1156 2573
Fragments arranged by size:
2573 1449 1156 546 502 471 408 383
191 189 170 131 46 38 25
BmgBI CAC'GTC
Cuts at: 1318 3705 1318
Size: 2387 5891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2502 2750 4562
Size: 416 53 83 399 24 1085 248 1812
Cuts at: 4562 4824 6009 6967 442
Size: 262 1185 958 1753
Fragments arranged by size:
1812 1753 1185 1085 958 416 399 262
248 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3975 1056
Size: 2919 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2086 2325 2516 2918 3041 3389 3455
Size: 800 47 239 191 402 123 348 66
Cuts at: 3455 3761 4146 4370 4415 5017 5325 5441 5491
Size: 306 385 224 45 602 308 116 50
Cuts at: 5491 5579 6938 1239
Size: 88 1359 2579
Fragments arranged by size:
2579 1359 800 602 402 385 348 308
306 239 224 191 123 116 88 66
50 47 45
Bpu10I CC'TnA_GC
Cuts at: 2342 2526 3242 3711 5427 5463 2342
Size: 184 716 469 1716 36 5157
Fragments arranged by size:
5157 1716 716 469 184 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3953 4751 6403 7268 7566 2446
Size: 1507 798 1652 865 298 3158
Fragments arranged by size:
3158 1652 1507 865 798 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2460 4949 5301 5352 5381 6941 882
Size: 617 961 2489 352 51 29 1560 2219
Fragments arranged by size:
2489 2219 1560 961 617 352 51 29
BsaAI yAC'GTr
Cuts at: 493 1204 6069 493
Size: 711 4865 2702
Fragments arranged by size:
4865 2702 711
BsaBI GATnn'nnATC
Cuts at: 4044 8272 4044
Size: 4228 4050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4740 4819 5240 6085
Size: 53 83 186 936 3207 79 421 845
Cuts at: 6085 6467 7102 275
Size: 382 635 1451
Fragments arranged by size:
3207 1451 936 845 635 421 382 186
83 79 53
BsaWI w'CCGG_w
Cuts at: 3955 6711 7109 7383 7530 7941 3955
Size: 2756 398 274 147 411 4292
Fragments arranged by size:
4292 2756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4147 4177 4226 4256 4559
Size: 30 165 30 2744 30 49 30 303
Cuts at: 4559 4589 4904 4934 1178
Size: 30 315 30 4522
Fragments arranged by size:
4522 2744 315 303 165 49 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2195 2263 2979 3452 3728 4143 4868
Size: 934 75 68 716 473 276 415 725
Cuts at: 4868 1186
Size: 4596
Fragments arranged by size:
4596 934 725 716 473 415 276 75
68
BseYI C'CCAG_C
Cuts at: 2224 2650 3090 3443 3558 3612 3642 3850 3882
Size: 426 440 353 115 54 30 208 32
Cuts at: 3882 5171 5362 5396 5437 7481 7977 2224
Size: 1289 191 34 41 2044 496 2525
Fragments arranged by size:
2525 2044 1289 496 440 426 353 208
191 115 54 41 34 32 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2692 3293 3374 3767 3984 4472
Size: 573 365 591 601 81 393 217 488
Cuts at: 4472 5330 1163
Size: 858 4111
Fragments arranged by size:
4111 858 601 591 573 488 393 365
217 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 5194 5728 6489 7517 1540
Size: 275 186 2041 1152 534 761 1028 2301
Fragments arranged by size:
2301 2041 1152 1028 761 534 275 186
BsiHKAI G_wGCw'C
Cuts at: 2779 4668 4701 6339 6424 7495 2779
Size: 1889 33 1638 85 1071 3562
Fragments arranged by size:
3562 1889 1638 1071 85 33
BsmI GAATG_Cn'
Cuts at: 5789 5882 5789
Size: 93 8185
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2432 2644 2779 2805 3052 3090
Size: 399 471 171 212 135 26 247 38
Cuts at: 3090 3248 3453 3592 3613 3838 3857 4138 4255
Size: 158 205 139 21 225 19 281 117
Cuts at: 4255 4308 4333 4524 4655 4668 4701 4890 5597
Size: 53 25 191 131 13 33 189 707
Cuts at: 5597 6339 6424 7495 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 471 399 281 247
225 212 205 191 189 171 158 139
135 131 117 85 53 38 33 26
25 21 19 13
BspEI T'CCGG_A
Cuts at: 7941 7941
Size: 8278
BspHI T'CATG_A
Cuts at: 6167 7897 6167
Size: 1730 6548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2715 3142 3406 3508 4012 4078 4590
Size: 1278 593 427 264 102 504 66 512
Cuts at: 4590 4734 4980 5085 5352 5633 844
Size: 144 246 105 267 281 3489
Fragments arranged by size:
3489 1278 593 512 504 427 281 267
264 246 144 105 102 66
BsrBI CCG'CTC
Cuts at: 1394 1823 4237 6165 1394
Size: 429 2414 1928 3507
Fragments arranged by size:
3507 2414 1928 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3270 5163 5244 6773 6947 65
Size: 1183 2022 1893 81 1529 174 1396
Fragments arranged by size:
2022 1893 1529 1396 1183 174 81
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 6922 7109 1700
Size: 110 2145 2967 187 2869
Fragments arranged by size:
2967 2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 5352 96
Size: 5256 3022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8278
BssSI C'ACGA_G
Cuts at: 4419 6338 7350 4419
Size: 1919 1012 5347
Fragments arranged by size:
5347 1919 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3047 3154 3775 4786 794
Size: 2253 107 621 1011 4286
Fragments arranged by size:
4286 2253 1011 621 107
BstBI TT'CG_AA
Cuts at: 7082 7082
Size: 8278
BstEII G'GTnAC_C
Cuts at: 1184 3235 1184
Size: 2051 6227
BstXI CCAn_nnnn'nTGG
Cuts at: 2584 3850 4340 5140 2584
Size: 1266 490 800 5722
Fragments arranged by size:
5722 1266 800 490
BstYI r'GATC_y
Cuts at: 1972 2513 4983 5475 5608 5975 6360 6377 7829
Size: 541 2470 492 133 367 385 17 1452
Cuts at: 7829 7915 7927 8273 1972
Size: 86 12 346 1977
Fragments arranged by size:
2470 1977 1452 541 492 385 367 346
133 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4019 4019
Size: 8278
BtgI C'CryG_G
Cuts at: 513 1065 2004 3096 3167 3843 4230 4318 4734
Size: 552 939 1092 71 676 387 88 416
Cuts at: 4734 4882 5133 513
Size: 148 251 3658
Fragments arranged by size:
3658 1092 939 676 552 416 387 251
148 88 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2426 487
Size: 683 56 1200 6339
Fragments arranged by size:
6339 1200 683 56
BtsI GCAGTG_nn'
Cuts at: 2631 4078 4369 4616 5794 6588 6608 2631
Size: 1447 291 247 1178 794 20 4301
Fragments arranged by size:
4301 1447 1178 794 291 247 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4879 4914 5063 5098 529
Size: 35 4315 35 149 35 3709
Fragments arranged by size:
4315 3709 149 35 35 35
DraI TTT'AAA
Cuts at: 5582 5582
Size: 8278
DraIII CAC_nnn'GTG
Cuts at: 2357 4953 2357
Size: 2596 5682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5198 7285 809
Size: 507 3882 2087 1802
Fragments arranged by size:
3882 2087 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 2586 3425
Size: 54 1425 50 275 18 744 12 839
Cuts at: 3425 3840 4092 4227 4731 5443 5725 6614 8243
Size: 415 252 135 504 712 282 889 1629
Cuts at: 8243 8
Size: 43
Fragments arranged by size:
1629 1425 889 839 744 712 504 415
282 275 252 135 54 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 5725 1537
Size: 275 3913 4090
Fragments arranged by size:
4090 3913 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2798 3692 4871 5373 6208 1353
Size: 744 701 894 1179 502 835 3423
Fragments arranged by size:
3423 1179 894 835 744 701 502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 3677 3857 4982 6865 7239
Size: 1064 138 1092 996 180 1125 1883 374
Cuts at: 7239 7385 387
Size: 146 1280
Fragments arranged by size:
1883 1280 1125 1092 1064 996 374 180
146 138
EcoICRI GAG'CTC
Cuts at: 4666 4666
Size: 8278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2086 2325 2516 2645 2891 2918
Size: 393 407 47 239 191 129 246 27
Cuts at: 2918 3041 3236 3389 3455 3673 3761 3965 4146
Size: 123 195 153 66 218 88 204 181
Cuts at: 4146 4370 4415 4852 5017 5234 5325 5441 5491
Size: 224 45 437 165 217 91 116 50
Cuts at: 5491 5579 6341 6938 7725 7993 1239
Size: 88 762 597 787 268 1524
Fragments arranged by size:
1524 787 762 597 437 407 393 268
246 239 224 218 217 204 195 191
181 165 153 129 123 116 91 88
88 66 50 47 45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2203 3056 4022 4142 4167 4850 2203
Size: 853 966 120 25 683 5631
Fragments arranged by size:
5631 966 853 683 120 25
EcoO109I rG'GnC_Cy
Cuts at: 2013 2137 2144 2297 2392 3139 3226 3346 3991
Size: 124 7 153 95 747 87 120 645
Cuts at: 3991 4312 4651 4652 5286 5334 5542 5554 2013
Size: 321 339 1 634 48 208 12 4737
Fragments arranged by size:
4737 747 645 634 339 321 208 153
124 120 95 87 48 12 7 1
EcoRI G'AATT_C
Cuts at: 3623 5587 5965 3623
Size: 1964 378 5936
Fragments arranged by size:
5936 1964 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4939 4939
Size: 8278
FspI TGC'GCA
Cuts at: 4811 5114 6784 4811
Size: 303 1670 6305
Fragments arranged by size:
6305 1670 303
FspAI rTGC'GCAy
Cuts at: 4811 5114 4811
Size: 303 7975
HaeII r_GCGC'y
Cuts at: 1678 4822 4921 7425 1678
Size: 3144 99 2504 2531
Fragments arranged by size:
3144 2531 2504 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2505 2537 3820
Size: 32 135 32 887 32 14 32 1283
Cuts at: 3820 3852 4559 4591 5114 5146 5300 5332 5465
Size: 32 707 32 523 32 154 32 133
Cuts at: 5465 5497 6919 6951 6993 7025 1373
Size: 32 1422 32 42 32 2626
Fragments arranged by size:
2626 1422 1283 887 707 523 154 135
133 42 32 32 32 32 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4762 4900 5203 5622 5868 669
Size: 988 281 2824 138 303 419 246 3079
Fragments arranged by size:
3079 2824 988 419 303 281 246 138
HindIII A'AGCT_T
Cuts at: 748 3929 748
Size: 3181 5097
HpaI GTT'AAC
Cuts at: 4762 5868 4762
Size: 1106 7172
KasI G'GCGC_C
Cuts at: 4818 4818
Size: 8278
KpnI G_GTAC'C
Cuts at: 5603 5603
Size: 8278
MfeI C'AATT_G
Cuts at: 5877 5877
Size: 8278
MluI A'CGCG_T
Cuts at: 8154 8154
Size: 8278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7392 7576 1305
Size: 6087 184 2007
Fragments arranged by size:
6087 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 2588 3427 3842 4229
Size: 54 1425 343 744 12 839 415 387
Cuts at: 4229 4733 10
Size: 504 3555
Fragments arranged by size:
3555 1425 839 744 504 415 387 343
54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 5018 5138 6236 6595 6754 518
Size: 648 3852 120 1098 359 159 2042
Fragments arranged by size:
3852 2042 1098 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2228 2896 3121 3550 3616 3646 3655 3775
Size: 622 668 225 429 66 30 9 120
Cuts at: 3775 3781 3882 3998 4207 4320 4708 4783 5063
Size: 6 101 116 209 113 388 75 280
Cuts at: 5063 5382 5526 5694 6371 7519 7764 1606
Size: 319 144 168 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 622 429 388 319
280 245 225 209 168 144 120 116
113 101 75 66 30 9 6
NarI GG'CG_CC
Cuts at: 4819 4819
Size: 8278
NcoI C'CATG_G
Cuts at: 513 1065 2004 3096 3167 4734 4882 5133 513
Size: 552 939 1092 71 1567 148 251 3658
Fragments arranged by size:
3658 1567 1092 939 552 251 148 71
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8278
NheI G'CTAG_C
Cuts at: 1052 3971 1052
Size: 2919 5359
NotI GC'GGCC_GC
Cuts at: 5725 5725
Size: 8278
NspI r_CATG'y
Cuts at: 1132 1229 3466 5636 7181 1132
Size: 97 2237 2170 1545 2229
Fragments arranged by size:
2237 2229 2170 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2372 2432 2828 3584 4309 5545 1348
Size: 1024 60 396 756 725 1236 4081
Fragments arranged by size:
4081 1236 1024 756 725 396 60
PciI A'CATG_T
Cuts at: 7177 7177
Size: 8278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 3097 3367 3658 4124 4603 1108
Size: 956 1033 270 291 466 479 4783
Fragments arranged by size:
4783 1033 956 479 466 291 270
PfoI T'CCnGG_A
Cuts at: 2204 5302 2204
Size: 3098 5180
PmeI GTTT'AAAC
Cuts at: 5582 5582
Size: 8278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4973 5005 6347 6379 7860 7892 4973
Size: 32 1342 32 1481 32 5359
Fragments arranged by size:
5359 1481 1342 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2144 2392 3139 3226 3991 5334 2137
Size: 7 248 747 87 765 1343 5081
Fragments arranged by size:
5081 1343 765 747 248 87 7
PshAI GACnn'nnGTC
Cuts at: 1613 2142 2397 3137 4941 1613
Size: 529 255 740 1804 4950
Fragments arranged by size:
4950 1804 740 529 255
PsiI TTA'TAA
Cuts at: 5848 5848
Size: 8278
PspOMI G'GGCC_C
Cuts at: 4651 4886 4651
Size: 235 8043
PstI C_TGCA'G
Cuts at: 830 2233 2895 3327 3414 3546 3780 4629 5028
Size: 1403 662 432 87 132 234 849 399
Cuts at: 5028 5630 830
Size: 602 3478
Fragments arranged by size:
3478 1403 849 662 602 432 399 234
132 87
PvuI CG_AT'CG
Cuts at: 2001 4042 2001
Size: 2041 6237
PvuII CAG'CTG
Cuts at: 2228 2896 3121 3616 3775 3781 4207 4708 4783
Size: 668 225 495 159 6 426 501 75
Cuts at: 4783 5063 5526 2228
Size: 280 463 4980
Fragments arranged by size:
4980 668 501 495 463 426 280 225
159 75 6
SacI G_AGCT'C
Cuts at: 4668 4668
Size: 8278
SacII CC_GC'GG
Cuts at: 4321 4321
Size: 8278
SalI G'TCGA_C
Cuts at: 1936 5620 1936
Size: 3684 4594
SanDI GG'GwC_CC
Cuts at: 2392 5334 2392
Size: 2942 5336
SapI GCTCTTCn'nnn_
Cuts at: 3692 4871 5373 3692
Size: 1179 502 6597
Fragments arranged by size:
6597 1179 502
SbfI CC_TGCA'GG
Cuts at: 5630 5630
Size: 8278
ScaI AGT'ACT
Cuts at: 6526 8238 6526
Size: 1712 6566
SexAI A'CCwGG_T
Cuts at: 2841 3501 2841
Size: 660 7618
SfcI C'TryA_G
Cuts at: 826 1045 2186 2229 2891 3026 3125 3253 3323
Size: 219 1141 43 662 135 99 128 70
Cuts at: 3323 3410 3542 3776 4030 4202 4474 4625 5024
Size: 87 132 234 254 172 272 151 399
Cuts at: 5024 5626 6761 7442 7633 826
Size: 602 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 681 662 602 399 272
254 234 219 191 172 151 135 132
128 99 87 70 43
SfoI GGC'GCC
Cuts at: 4820 4820
Size: 8278
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8278
SgrAI Cr'CCGG_yG
Cuts at: 1700 3955 1700
Size: 2255 6023
SmaI CCC'GGG
Cuts at: 1872 3377 3756 5605 1872
Size: 1505 379 1849 4545
Fragments arranged by size:
4545 1849 1505 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 3932 4766 6382 7283 7545
Size: 197 926 482 1507 834 1616 901 262
Cuts at: 7545 820
Size: 1553
Fragments arranged by size:
1616 1553 1507 926 901 834 482 262
197
SnaBI TAC'GTA
Cuts at: 493 6069 493
Size: 5576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8278
SphI G_CATG'C
Cuts at: 1132 5636 1132
Size: 4504 3774
SspI AAT'ATT
Cuts at: 5 52 6202 5
Size: 47 6150 2081
Fragments arranged by size:
6150 2081 47
StuI AGG'CCT
Cuts at: 1517 2970 2990 3698 4028 5030 5054 5153 1517
Size: 1453 20 708 330 1002 24 99 4642
Fragments arranged by size:
4642 1453 1002 708 330 99 24 20
StyI C'CwwG_G
Cuts at: 513 1065 2004 2461 2614 3096 3167 3478 4734
Size: 552 939 457 153 482 71 311 1256
Cuts at: 4734 4882 4939 5133 5717 513
Size: 148 57 194 584 3074
Fragments arranged by size:
3074 1256 939 584 552 482 457 311
194 153 148 71 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 3104 4428 5126 6305 6322 6475
Size: 1255 606 918 1324 698 1179 17 153
Cuts at: 6475 6660 7105 325
Size: 185 445 1498
Fragments arranged by size:
1498 1324 1255 1179 918 698 606 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2731 4260 5352
Size: 274 80 33 51 1443 754 1529 1092
Cuts at: 5352 6524 8236 96
Size: 1172 1712 138
Fragments arranged by size:
1712 1529 1443 1172 1092 754 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2736 2768 8238 8270 2736
Size: 32 5470 32 2744
Fragments arranged by size:
5470 2744 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8278
XbaI T'CTAG_A
Cuts at: 4255 5614 4255
Size: 1359 6919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3174 4600 3174
Size: 1426 6852
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8278
XmaI C'CCGG_G
Cuts at: 1870 3375 3754 5603 1870
Size: 1505 379 1849 4545
Fragments arranged by size:
4545 1849 1505 379
XmnI GAAnn'nnTTC
Cuts at: 4277 6407 4277
Size: 2130 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6086 276
Size: 53 83 186 936 4552 2468
Fragments arranged by size:
4552 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FseI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SfiI SrfI SwaI