Restriction map for
pFN21AF0052
(Circular) (Six-base) MAPSORT of: pFN21AF0052.seq Check: 2986 from: 1 to: 5689
pFN21AF0052 5689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 2808 3010 2808
Size: 202 5487
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3752 5136 5404 1632
Size: 1301 819 1384 268 1917
Fragments arranged by size:
1917 1384 1301 819 268
AflII C'TTAA_G
Cuts at: 820 1017 2681 820
Size: 197 1664 3828
Fragments arranged by size:
3828 1664 197
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2745 1184
Size: 1561 4128
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 2337
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 2857 2987 3432 5004 2857
Size: 130 445 1572 3542
Fragments arranged by size:
3542 1572 445 130
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2417 2998 3224 3376 5485 1493
Size: 924 581 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 581 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2075 3014 1870
Size: 73 120 12 939 4545
Fragments arranged by size:
4545 939 120 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2204 2237 2204
Size: 33 5656
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2808 3010 4465 618
Size: 325 217 627 1021 202 1455 1842
Fragments arranged by size:
1842 1455 1021 627 325 217 202
BanII G_rGCy'C
Cuts at: 1391 2961 3008 1391
Size: 1570 47 4072
Fragments arranged by size:
4072 1570 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 2199 2430 2912 928
Size: 971 252 48 231 482 3705
Fragments arranged by size:
3705 971 482 252 231 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2325 3581 4791 2325
Size: 1256 1210 3223
Fragments arranged by size:
3223 1256 1210
BclI T'GATC_A
Cuts at: 1548 2868 1548
Size: 1320 4369
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2892 5684 2892
Size: 2792 2897
BlpI GC'TnA_GC
Cuts at: 2460 2980 3106 2460
Size: 520 126 5043
Fragments arranged by size:
5043 520 126
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2114 2339 2538 2598 2733 2855 4349
Size: 828 47 225 199 60 135 122 1494
Cuts at: 4349 1239
Size: 2579
Fragments arranged by size:
2579 1494 828 225 199 135 122 60
47
Bpu10I CC'TnA_GC
Cuts at: 2718 2718
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 3814 4679 4977 2228
Size: 1586 865 298 2940
Fragments arranged by size:
2940 1586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2689 4352 882
Size: 617 1190 1663 2219
Fragments arranged by size:
2219 1663 1190 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 2930 5683 2930
Size: 2753 2936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 1178
Size: 30 165 30 934 30 4500
Fragments arranged by size:
4500 934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2614 1186
Size: 907 521 4261
Fragments arranged by size:
4261 907 521
BseYI C'CCAG_C
Cuts at: 2619 4892 5388 2619
Size: 2273 496 2920
Fragments arranged by size:
2920 2273 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3750 3835 4906 2724
Size: 1026 85 1071 3507
Fragments arranged by size:
3507 1071 1026 85
BsmI GAATG_Cn'
Cuts at: 2344 3200 3293 2344
Size: 856 93 4740
Fragments arranged by size:
4740 856 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2724 2961 3008 3750 3835 4906 1391
Size: 399 934 237 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 934 742 399 237 85 47
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2544 3578 5308 2544
Size: 1034 1730 2925
Fragments arranged by size:
2925 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 3044 844
Size: 1773 427 3489
Fragments arranged by size:
3489 1773 427
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2973 4333 4520 1700
Size: 110 246 917 1360 187 2869
Fragments arranged by size:
2869 1360 917 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 794
Size: 1469 4220
BstBI TT'CG_AA
Cuts at: 2763 4493 2763
Size: 1730 3959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2659 2892 2931 3019 3386 3771 3788 5240
Size: 687 233 39 88 367 385 17 1452
Cuts at: 5240 5326 5338 5684 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 687 385 367 346 233 88
86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2013 2739 513
Size: 552 948 726 3463
Fragments arranged by size:
3463 948 726 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2415 3205 3999 4019 2415
Size: 790 794 20 4085
Fragments arranged by size:
4085 794 790 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2288 2354 2966 3619 1353
Size: 729 206 66 612 653 3423
Fragments arranged by size:
3423 729 653 612 206 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2960 4276 4650 4796 387
Size: 1064 138 1371 1316 374 146 1280
Fragments arranged by size:
1371 1316 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2114 2339 2538 2598 2733 2855
Size: 393 435 47 225 199 60 135 122
Cuts at: 2855 2933 3752 4349 5136 5404 1239
Size: 78 819 597 787 268 1524
Fragments arranged by size:
1524 819 787 597 435 393 268 225
199 135 122 78 60 47
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2054 2103 2980 4836 1678
Size: 376 49 877 1856 2531
Fragments arranged by size:
2531 1856 877 376 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373
Size: 32 135 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 3033 3279 669
Size: 988 281 699 396 246 3079
Fragments arranged by size:
3079 988 699 396 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 748
Size: 1930 3759
HpaI GTT'AAC
Cuts at: 2637 3279 2637
Size: 642 5047
KpnI G_GTAC'C
Cuts at: 2812 3014 2812
Size: 202 5487
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 4803 4987 1305
Size: 813 2685 184 2007
Fragments arranged by size:
2685 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 3647 4006 4165 518
Size: 648 1758 723 359 159 2042
Fragments arranged by size:
2042 1758 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2121 2359 2741 2984 3105 3782 4930
Size: 409 106 238 382 243 121 677 1148
Cuts at: 4930 5175 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 409 382 245 243 238
121 106
NaeI GCC'GGC
Cuts at: 2058 2975 2058
Size: 917 4772
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2056 2973 2056
Size: 917 4772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2337 2369 3758 3790 5271 5303 2337
Size: 32 1389 32 1481 32 2723
Fragments arranged by size:
2723 1481 1389 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2121 2359 2984 2121
Size: 238 625 4826
Fragments arranged by size:
4826 625 238
SacII CC_GC'GG
Cuts at: 2016 2742 2016
Size: 726 4963
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2082 2966 2082
Size: 884 4805
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2069 3937 5649 2069
Size: 1868 1712 2109
Fragments arranged by size:
2109 1868 1712
SfcI C'TryA_G
Cuts at: 826 1045 2877 3037 4172 4853 5044 826
Size: 219 1832 160 1135 681 191 1471
Fragments arranged by size:
1832 1471 1135 681 219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 2973 1700
Size: 1273 4416
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2681 3793 4694 4956 820
Size: 197 926 300 438 1112 901 262 1553
Fragments arranged by size:
1553 1112 926 901 438 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2704 2887 3716 3733 3886 4071 4516
Size: 1255 1124 183 829 17 153 185 445
Cuts at: 4516 325
Size: 1498
Fragments arranged by size:
1498 1255 1124 829 445 185 183 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2319 2790
Size: 274 80 33 51 1443 90 252 471
Cuts at: 2790 3935 5647 96
Size: 1145 1712 138
Fragments arranged by size:
1712 1443 1145 471 274 252 138 90
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2896 2928 5649 5681 2896
Size: 32 2721 32 2904
Fragments arranged by size:
2904 2721 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI
XcmI