Restriction map for
pFN21AF0017
(Circular) (Six-base) MAPSORT of: pFN21AF0017.seq Check: 6124 from: 1 to: 5887
pFN21AF0017 5887 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2564 2564
Size: 5887
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2283 3697 278
Size: 53 83 186 936 747 1414 2468
Fragments arranged by size:
2468 1414 936 747 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 2339 3208 2339
Size: 869 5018
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2132 3950 5334 5602 1632
Size: 500 1818 1384 268 1917
Fragments arranged by size:
1917 1818 1384 500 268
AfeI AGC'GCT
Cuts at: 2639 2639
Size: 5887
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2562 4786 5763 1203
Size: 1359 2224 977 1327
Fragments arranged by size:
2224 1359 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 2092 2110 2446 3630 5202 2092
Size: 18 336 1184 1572 2777
Fragments arranged by size:
2777 1572 1184 336 18
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 3196 3422 3574 5683 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 3212 1870
Size: 73 741 528 4545
Fragments arranged by size:
4545 741 528 73
BamHI G'GATC_C
Cuts at: 2359 3217 3584 2359
Size: 858 367 4662
Fragments arranged by size:
4662 858 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 2339 3208 4663 618
Size: 325 217 627 289 263 869 1455 1842
Fragments arranged by size:
1842 1455 869 627 325 289 263 217
BanII G_rGCy'C
Cuts at: 1391 2028 2976 3206 1391
Size: 637 948 230 4072
Fragments arranged by size:
4072 948 637 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 2831 928
Size: 971 631 301 3984
Fragments arranged by size:
3984 971 631 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2252 3779 4989 2252
Size: 1527 1210 3150
Fragments arranged by size:
3150 1527 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 2036 3304 2036
Size: 1268 4619
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 2829 1318
Size: 1511 4376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4576 442
Size: 416 53 83 399 24 2201 958 1753
Fragments arranged by size:
2201 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2944 2749
Size: 195 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2366 2990 4547 1239
Size: 875 252 624 1557 2579
Fragments arranged by size:
2579 1557 875 624 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2939 4012 4877 5175 2789
Size: 150 1073 865 298 3501
Fragments arranged by size:
3501 1073 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3157 4550 882
Size: 617 1658 1393 2219
Fragments arranged by size:
2219 1658 1393 617
BsaAI yAC'GTr
Cuts at: 493 1204 2563 3678 493
Size: 711 1359 1115 2702
Fragments arranged by size:
2702 1359 1115 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 2535 3694 4076
Size: 53 83 186 936 747 255 1159 382
Cuts at: 4076 4711 275
Size: 635 1451
Fragments arranged by size:
1451 1159 936 747 635 382 255 186
83 53
BsaWI w'CCGG_w
Cuts at: 2487 4320 4718 4992 5139 5550 2487
Size: 1833 398 274 147 411 2824
Fragments arranged by size:
2824 1833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 1186
Size: 994 4893
BseYI C'CCAG_C
Cuts at: 5090 5586 5090
Size: 496 5391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 1163
Size: 573 1218 4096
Fragments arranged by size:
4096 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2976 3948 4033 5104 2262
Size: 714 972 85 1071 3045
Fragments arranged by size:
3045 1071 972 714 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
BsmBI CGTCTCn'nnnn_
Cuts at: 2042 2042
Size: 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2262 2976 3206 3948 4033 5104
Size: 399 238 234 714 230 742 85 1071
Cuts at: 5104 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 714 399 238 234 230
85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 2379 2564 2688 3175 3242 844
Size: 1521 14 185 124 487 67 3489
Fragments arranged by size:
3489 1521 487 185 124 67 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2761 4382 4556 65
Size: 1183 1041 472 1621 174 1396
Fragments arranged by size:
1621 1396 1183 1041 472 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 2402 3947 4959 2402
Size: 1545 1012 3330
Fragments arranged by size:
3330 1545 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 4415
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2359 3217 3584 3969 3986 5438 5524 5536
Size: 387 858 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 858 387 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 2742 2435
Size: 307 5580
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2656 2900 513
Size: 552 939 24 628 244 3500
Fragments arranged by size:
3500 939 628 552 244 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2711 3817 1353
Size: 696 662 1106 3423
Fragments arranged by size:
3423 1106 696 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2974 2974
Size: 5887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2132 2366 2990 3950 4547 5334
Size: 393 482 18 234 624 960 597 787
Cuts at: 5334 5602 1239
Size: 268 1524
Fragments arranged by size:
1524 960 787 624 597 482 393 268
234 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 2684 2426
Size: 258 5629
EcoO109I rG'GnC_Cy
Cuts at: 2317 2377 2431 2317
Size: 60 54 5773
Fragments arranged by size:
5773 60 54
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2641 2842 5034 1678
Size: 963 201 2192 2531
Fragments arranged by size:
2531 2192 963 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2505 2537 3108 3137 3140
Size: 32 135 32 933 32 571 29 3
Cuts at: 3140 3169 4528 4560 4602 4634 1373
Size: 29 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 933 571 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 2343 3212 2343
Size: 869 5018
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3845 4204 4363 518
Size: 648 1416 1263 359 159 2042
Fragments arranged by size:
2042 1416 1263 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2043 2658 2695 3303 3980 5128 5373
Size: 424 13 615 37 608 677 1148 245
Cuts at: 5373 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 615 608 424 245 37
13
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PfoI T'CCnGG_A
Cuts at: 2091 2283 2616 2091
Size: 192 333 5362
Fragments arranged by size:
5362 333 192
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2377
Size: 5887
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2376 3239 830
Size: 1546 863 3478
Fragments arranged by size:
3478 1546 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2695 2695
Size: 5887
SacI G_AGCT'C
Cuts at: 2976 2976
Size: 5887
SacII CC_GC'GG
Cuts at: 2031 2659 2031
Size: 628 5259
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 2376 3239 2376
Size: 863 5024
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SexAI A'CCwGG_T
Cuts at: 3147 3147
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 2372 3235 4370 5051 5242 826
Size: 219 1327 863 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 863 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 2918 3991 4892 5154 820
Size: 197 926 861 114 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 861 262 197 114
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 2501 3011 1517
Size: 870 114 510 4393
Fragments arranged by size:
4393 870 510 114
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 3326 513
Size: 552 939 231 1091 3074
Fragments arranged by size:
3074 1091 939 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2034 2870 3035 3914 3931 4084 4269
Size: 1255 454 836 165 879 17 153 185
Cuts at: 4269 4714 325
Size: 445 1498
Fragments arranged by size:
1498 1255 879 836 454 445 185 165
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 3149 1316
Size: 1833 4054
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2705 2960 4016 2705
Size: 255 1056 4576
Fragments arranged by size:
4576 1056 255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2281 3695 276
Size: 53 83 186 936 747 1414 2468
Fragments arranged by size:
2468 1414 936 747 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SfoI SrfI SwaI XcmI